LOCUS Exported 6179 bp ds-DNA circular SYN 01-DEC-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS FP1236_pCAG cyto-RCaMP1h SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6179) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Dec 1, 2017 from SnapGene 3.1.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6179 /organism="synthetic DNA construct" /mol_type="other DNA" source 3544..4872 /organism="pRSET-RCaMP1h" /mol_type="other DNA" /label=source:pRSET-RCaMP1h /note="source:pRSET-RCaMP1h" CDS complement(803..1462) /label=AmpR /note="AmpR" misc_feature 1810..3421 /label=CAG promoter /note="CAG promoter" CDS 3544..4872 /codon_start=1 /label=ORF frame 1 /note="ORF frame 1" /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK TGHAVRAIGRLSSAINTEMMYPADGGLRGYTHMALKVDGGGHLSCSFVTTYRSKKTVGN IKMPAIHYVSHRLERLEESDNEMFVVQREHAVAKFVGLGGGGGTGGSMNSLIKENMRMK VVLEGSVNGHQFKCTGEGEGNPYMGTQTMRIKVIEGGPLPFAFDILATSFMYGSRTFIK YPKGIPDFFKQSFPEGFTWERVTRYEDGGVITVMQDTSLEDGCLVYHVQVRGVNFPSNG AVMQKKTKGWEPTDSQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA ELQDMINEVDADGDGTIDFPEFLIMMARKMKYTDSEEEIREAFGVFDKDGNGYISAAEL RHVMTNLGEKLTDEEVDEMIREADSDGDGQVNYEEFVQMMTAK" misc_feature 3553..3570 /label=6xHIS /note="6xHIS" misc_feature 3573..3591 /label=Xpress_fwd_primer /note="Xpress_fwd_primer" misc_feature 3574..3606 /label=T7_leader /note="T7_leader" misc_feature 3574..3606 /label=T7 Tag /note="T7 Tag" misc_feature 3610..3633 /label=Xpress_EK /note="Xpress_EK" primer_bind complement(5470..5490) /label=M13-rev /note="M13-rev" misc_binding complement(5496..5518) /label=LacO /note="LacO" ORIGIN 1 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 61 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 121 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 181 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 241 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 301 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 361 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 421 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 481 agacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 541 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 601 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 661 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 721 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 781 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 841 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 901 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 961 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 1021 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 1081 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 1141 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 1201 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 1261 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 1321 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 1381 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 1441 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 1501 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 1561 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 1621 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 1681 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 1741 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcgacat 1801 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat 1861 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 1921 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 1981 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg 2041 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 2101 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 2161 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc 2221 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg 2281 gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg 2341 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt 2401 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc 2461 gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc gccccggctc 2521 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt 2581 aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga aagccttaaa 2641 gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt gcgtgtgtgt 2701 gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg ctgcgggcgc 2761 ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg ggcggtgccc 2821 cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg 2881 gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac ccccctcccc 2941 gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc 3001 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc 3061 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag 3121 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc 3181 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg 3241 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg 3301 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg 3361 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct 3421 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg 3481 tgctggttat tgtgctgtct catcattttg gcaaagaatt gctcgagctc aagcttcgaa 3541 ttcatgggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa 3601 atgggtcggg atctgtacga cgatgacgat aaggatctcg ccaccatggt cgactcatca 3661 cgtcgtaagt ggaataagac aggtcacgca gtcagagcta taggtcggct gagctcagcg 3721 atcaacaccg aaatgatgta cccagcggat ggtggtctgc gtggttacac tcacatggcg 3781 ctgaaagttg atggcggcgg tcacctgtcc tgttctttcg tgaccaccta ccgctccaaa 3841 aagactgtcg gcaacattaa gatgcctgcc attcattacg tcagccaccg tctggagcgc 3901 ctggaggaga gcgataacga aatgtttgtc gtacagcgtg aacacgcagt tgccaagttt 3961 gtgggcctgg gtggtggcgg cggtaccgga gggagcatga actccctgat caaggagaac 4021 atgcgtatga aagtggttct ggaaggctcc gtaaacggcc accagttcaa atgcactggt 4081 gaaggcgaag gcaacccgta tatgggcacc cagactatgc gtatcaaagt gatcgagggt 4141 ggtccgctgc cgtttgcgtt cgacatcctg gcgacgtcct ttatgtatgg ctcccgtacc 4201 ttcatcaaat atccgaaagg catcccggat ttctttaagc agtccttccc ggaaggtttt 4261 acctgggaac gtgtgacccg ttacgaagac ggcggcgtaa ttaccgttat gcaagacacg 4321 tctctggagg atggctgcct ggtgtatcac gtgcaggttc gcggtgttaa cttcccgagc 4381 aatggtgctg taatgcaaaa gaaaaccaaa ggttgggagc ctacggactc ccaactgact 4441 gaagagcaga tcgcagaatt taaagaggct ttctccctat ttgacaagga cggggatggg 4501 acaataacaa ccaaggagct ggggacggtg atgcggtctc tggggcagaa ccccacagaa 4561 gcagagctgc aggacatgat caatgaagta gatgccgacg gtgacggcac aatcgacttc 4621 cctgagttcc tgattatgat ggcaagaaaa atgaaataca cagacagtga agaagaaatt 4681 agagaagcgt tcggcgtgtt tgataaggat ggcaatggct acatcagtgc agcagagctt 4741 cgccacgtga tgacaaacct tggagagaag ttaacagatg aagaggttga tgaaatgatc 4801 agggaagcag acagcgatgg ggatggtcag gtaaactacg aagagtttgt acaaatgatg 4861 acagcgaagt aagcggccgc aattcactcc tcaggtgcag gctgcctatc agaaggtggt 4921 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa 4981 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt 5041 tattttcatt gcaatagtgt gttggaattt tttgtgtcta tcactcggaa ggacatatgg 5101 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc 5161 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca 5221 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt 5281 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt 5341 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt 5401 atggagatcc ctcgacctgc accgtcgacc agctggtcga cggtgcaccg tcgaccagct 5461 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 5521 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 5581 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 5641 ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 5701 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 5761 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 5821 tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa 5881 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 5941 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg 6001 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 6061 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 6121 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccag //