LOCUS       pNX32-DEST         8496 bp    DNA     circular     14-JAN-2009
SOURCE      
  ORGANISM  
REFERENCE   
  AUTHORS   Christopher Grefen
FEATURES             Location/Qualifiers
     rep_origin      2691..3860
                     /label=pUC\ORI
     CDS             6738..7412
                     /label=TRP1
     rep_origin      7463..8127
                     /label=f1\ORI
     promoter        1..357
                     /label=ADH
     CDS             363..473
                     /label=NubG
     misc_feature    483..607
                     /label=attR1
     CDS             880..1539
                     /label=CmR
     CDS             1881..2186
                     /label=ccdB
     misc_feature    complement(2227..2351)
                     /label=attR2
     CDS             2365..2454
                     /label=3HA
     CDS             complement(3862..4722)
                     /label=AmpR
     rep_origin      4853..6200
                     /label=2µ\ORI
BASE COUNT     2350 a      1827 c      1957 g      2362 t 
ORIGIN
        1 tgttgtctca ccatatccgc aatgacaaaa aaatgatgga agacactaaa ggaaaaaatt 
       61 aacgacaaag acagcaccaa cagatgtcgt tgttccagag ctgatgaggg gtatctcgaa 
      121 gcacacgaaa ctttttcctt ccttcattca cgcacactac tctctaatga gcaacggtat 
      181 acggccttcc ttccagttac ttgaatttga aataaaaaaa agtttgctgt cttgctatca 
      241 agtataaata gacctgcaat tattaatctt ttgtttcctc gtcattgttc tcgttccctt 
      301 tcttccttgt ttctttttct gcacaatatt tcaagctata ccaagcatac aatcaactcg 
      361 agatgcagat tttcgtcaag actttgaccg gtaaaaccgg aacattggaa gttgaatctt 
      421 ccgataccat cgacaacgtt aagtcgaaaa ttcaagacaa ggaaggaatc cctggtgggt 
      481 cgacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat 
      541 taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt 
      601 cactatggcg gccgcgggtg atgctgccaa cttagcggcc gtaagttgcg atcacccgac 
      661 gcactttgcg ccgaataaat acctgtgacg gaagatcact tcgcagaata aataaatcct 
      721 ggtgtccctg ttgataccgg gaagccctgg gccaactttt ggcgaaaatg agacgttgat 
      781 cggcacgcaa gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt 
      841 tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga 
      901 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca 
      961 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc 
     1021 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg 
     1081 aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt 
     1141 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt 
     1201 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac 
     1261 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc 
     1321 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc 
     1381 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg 
     1441 gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa 
     1501 ttacaacagt actgcgatga gtggcagggc ggggcgtaaa cgcgtggatc cggcttacta 
     1561 aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact 
     1621 gatatgtata cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt attacagtga 
     1681 cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct 
     1741 ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga 
     1801 aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga 
     1861 cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt 
     1921 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga 
     1981 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg 
     2041 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct 
     2101 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca 
     2161 ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag 
     2221 gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc 
     2281 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg 
     2341 tacaaagtgg ttggtggtgg cggataccca tacgatgttc ctgactatgc gggctatccc 
     2401 tatgacgtcc cggactatgc aggatcctat ccatatgacg ttccagatta cgctgctcag 
     2461 tgctgaggcg cgccacttct aaataagcga atttcttatg atttatgatt tttattatta 
     2521 aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac 
     2581 gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagt 
     2641 atgaggtcgc tcttattgac cacacctcta ccggcagcat gcaagcttgg cgtaatcatg 
     2701 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 
     2761 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 
     2821 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 
     2881 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 
     2941 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 
     3001 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 
     3061 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 
     3121 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 
     3181 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 
     3241 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 
     3301 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 
     3361 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 
     3421 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 
     3481 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 
     3541 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 
     3601 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 
     3661 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 
     3721 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 
     3781 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 
     3841 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 
     3901 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 
     3961 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 
     4021 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 
     4081 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 
     4141 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 
     4201 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 
     4261 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 
     4321 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 
     4381 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 
     4441 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 
     4501 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 
     4561 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 
     4621 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 
     4681 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 
     4741 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 
     4801 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgaacgaag 
     4861 catctgtgct tcattttgta gaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa 
     4921 agaatctgag ctgcattttt acagaacaga aatgcaacgc gaaagcgcta ttttaccaac 
     4981 gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc taatttttca 
     5041 aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag cgctatttta 
     5101 ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag agcgctattt 
     5161 ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata atgcagtctc 
     5221 ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt tggtgtctat 
     5281 tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta ctagcgaagc 
     5341 tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc gatgtggatt 
     5401 gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt cagaaaatta 
     5461 tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt acattttcgt 
     5521 attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct aaagagtaat 
     5581 actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca aggagcgaaa 
     5641 ggtggatggg taggttatat agggatatag cacagagata tatagcaaag agatactttt 
     5701 gagcaatgtt tgtggaagcg gtattcgcaa tattttagta gctcgttaca gtccggtgcg 
     5761 tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag cgctctgaag 
     5821 ttcctatact ttctagagaa taggaacttc ggaataggaa cttcaaagcg tttccgaaaa 
     5881 cgagcgcttc cgaaaatgca acgcgagctg cgcacataca gctcactgtt cacgtcgcac 
     5941 ctatatctgc gtgttgcctg tatatatata tacatgagaa gaacggccat agtgcgtgtt 
     6001 tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg atgaaaggta gtctagtacc 
     6061 tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc ttccttcagc actacccttt 
     6121 agctgttcta tatgctgcca ctcctcaatt ggattagtct catccttcaa tgctatcatt 
     6181 tcctttgata ttggatcata ctaagaaacc attattatca tgacattaac ctataaaaat 
     6241 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga 
     6301 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa 
     6361 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca 
     6421 tcagagcaga ttgtactgag agtgcaccat agatcaacga cattactata tatataatat 
     6481 aggaagcatt taatagaaca gcatcgtaat atatgtgtac tttgcagtta tgacgccaga 
     6541 tggcagtagt ggaagatatt ctttattgaa aaatagcttg tcaccttacg tacaatcttg 
     6601 atccggagct tttctttttt tgccgattaa gaattaattc ggtcgaaaaa agaaaaggag 
     6661 agggccaaga gggagggcat tggtgactat tgagcacgtg agtatacgtg attaagcaca 
     6721 caaaggcagc ttggagtatg tctgttatta atttcacagg tagttctggt ccattggtga 
     6781 aagtttgcgg cttgcagagc acagaggccg cagaatgtgc tctagattcc gatgctgact 
     6841 tgctgggtat tatatgtgtg cccaatagaa agagaacaat tgacccggtt attgcaagga 
     6901 aaatttcaag tcttgtaaaa gcatataaaa atagttcagg cactccgaaa tacttggttg 
     6961 gcgtgtttcg taatcaacct aaggaggatg ttttggctct ggtcaatgat tacggcattg 
     7021 atatcgtcca actgcatgga gatgagtcgt ggcaagaata ccaagagttc ctcggtttgc 
     7081 cagttattaa aagactcgta tttccaaaag actgcaacat actactcagt gcagcttcac 
     7141 agaaacctca ttcgtttatt cccttgtttg attcagaagc aggtgggaca ggtgaacttt 
     7201 tggattggaa ctcgatttct gactgggttg gaaggcaaga gagccccgaa agcttacatt 
     7261 ttatgttagc tggtggactg acgccagaaa atgttggtga tgcgcttaga ttaaatggcg 
     7321 ttattggtgt tgatgtaagc ggaggtgtgg agacaaatgg tgtaaaagac tctaacaaaa 
     7381 tagcaaattt cgtcaaaaat gctaagaaat aggttattac tgagtagtat ttatttaagt 
     7441 attgtttgtg cacttgccga tctatgcggt gtgaaatacc gcacagatgc gtaaggagaa 
     7501 aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg 
     7561 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa 
     7621 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa 
     7681 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg 
     7741 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa 
     7801 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa 
     7861 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct 
     7921 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca 
     7981 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag 
     8041 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag 
     8101 tcacgacgtt gtaaaacgac ggccagtgcc aagctttcgc gagctcgata tccttttgtt 
     8161 gtttccgggt gtacaatatg gacttcctct tttctggcaa ccaaacccat acatcgggat 
     8221 tcctataata ccttcgttgg tctccctaac atgtaggtgg cggaggggag atatacaata 
     8281 gaacagatac cagacaagac ataatgggct aaacaagact acaccaatta cactgcctca 
     8341 ttgatggtgg tacataacga actaatactg tagccctaga cttgatagcc atcatcatat 
     8401 cgaagtttca ctaccctttt tccatttgcc atctattgaa gtaataatag gcgcatgcaa 
     8461 cttcttttct ttttttttct tttctctctc ccccgt 
//
