LOCUS       pUC-R3R2         3289 bp    DNA     circular     25-SEP-2011
DEFINITION  2in1 vector conversion kit.
KEYWORDS    2in1.
SOURCE      
  ORGANISM  
REFERENCE   
  AUTHORS   Grefen,C., and Blatt,M.R.
  TITLE     A 2in1 cloning system enables ratiometric bimolecular fluorescence complementation (rBiFC).
  JOURNAL   Biotechniques. 2012 Oct 2;53(5):311-14.
   PUBMED   23066669
FEATURES             Location/Qualifiers
     rep_origin      complement(1455..2069)
                     /label=pMDB1
     CDS             complement(2229..3089)
                     /label=AmpR
     misc_recomb     414..538
                     /label=attR3
     CDS             647..865
                     /label=lacZ
     promoter        549..646
                     /label=PlacZ
     misc_recomb     complement(912..1036)
                     /label=attR2
BASE COUNT      836 a       808 c       805 g       840 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cactagtcaa ttggcaactt 
      421 tgtataataa agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga 
      481 ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag tcactatggc 
      541 ggccgcgggc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 
      601 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac 
      661 cccgtccctg cacgcctgtc gttcaaccct ggaagatccg cgtgtcccga gttctaacag 
      721 tctggccgtc gttctgcagc gtcgcgattg ggaaaacccg ggcgtgaccc aactgaatcg 
      781 tctggcggcc catccgccgt ttgcgtcgtg gcgtaactca gaagaagccc gtaccgaccg 
      841 tccgtctcaa caactgcgct cctaacacta taaaaagctg atgcagacgg ggcttttttc 
      901 tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt 
      961 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt 
     1021 tcttgtacaa agtggtgtct aggttataaa agcttggcgt aatcatggtc atagctgttt 
     1081 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 
     1141 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 
     1201 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 
     1261 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 
     1321 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 
     1381 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 
     1441 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 
     1501 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 
     1561 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 
     1621 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 
     1681 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 
     1741 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 
     1801 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 
     1861 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 
     1921 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 
     1981 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 
     2041 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 
     2101 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 
     2161 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 
     2221 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 
     2281 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 
     2341 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 
     2401 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 
     2461 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 
     2521 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 
     2581 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 
     2641 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 
     2701 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 
     2761 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 
     2821 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 
     2881 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 
     2941 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 
     3001 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 
     3061 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 
     3121 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 
     3181 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt 
     3241 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc 
//
