LOCUS       Exported                9114 bp ds-DNA     circular SYN 02-JUN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    SnapGene DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 2, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     GenBank       9114 bp    DNA     circular SYN 29-MAY-2017
            ACCESSION   pLN351
            KEYWORDS:   .
FEATURES             Location/Qualifiers
     source          1..9114
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(121..708)
                     /gene="WPRE"
                     /product="The WPRE seems to increase the expression of the 
                     marker gene when introduced in its 3' untranslated region. 
                     This element is believed to exert some posttranslational 
                     regulation. "
                     /label=WPRE
                     /note="The WPRE seems to increase the expression of the 
                     marker gene when introduced in its 3' untranslated region. 
                     This element is believed to exert some posttranslational 
                     regulation."
     CDS             751..763
                     /gene="YBX1 "
                     /product="Gencards"
                     /label=YBX1
                     /note="Gencards"
     CDS             766..778
                     /gene="YBX1 "
                     /product="Gencards"
                     /label=YBX1
                     /note="Gencards"
     CDS             781..793
                     /gene="YBX1 "
                     /product="Gencards"
                     /label=YBX1
                     /note="Gencards"
     misc_signal     796..808
                     /label=YBX1
                     /label=YBX1
                     /note="Gencards"
     misc_signal     811..823
                     /label=YBX1
                     /label=YBX1
                     /note="Gencards"
     misc_signal     827..838
                     /label=YBX1
                     /label=YBX1
                     /note="Gencards"
     misc_signal     843..855
                     /label=YBX1
                     /label=YBX1
                     /note="Gencards"
     misc_signal     877..896
                     /label=20BP LINKER
     misc_signal     900..912
                     /label=YBX1
                     /label=YBX1
                     /note="Gencards"
     misc_signal     915..927
                     /label=YBX1
                     /label=YBX1
                     /note="Gencards"
     misc_signal     930..942
                     /label=YBX1
                     /label=YBX1
                     /note="Gencards"
     misc_signal     945..957
                     /label=YBX1
                     /label=YBX1
                     /note="Gencards"
     misc_signal     960..972
                     /label=YBX1
                     /label=YBX1
                     /note="Gencards"
     misc_signal     975..987
                     /label=YBX1
                     /label=YBX1
                     /note="Gencards"
     misc_signal     990..1002
                     /label=YBX1
                     /label=YBX1
                     /note="Gencards"
     misc_signal     1005..1017
                     /label=YBX1
                     /label=YBX1
                     /note="Gencards"
     CDS             1036..1086
                     /gene="lateADEp"
                     /product="major late promoter of adenovirus"
                     /label=lateADEp
                     /note="major late promoter of adenovirus"
     misc_feature    1087..1132
                     /gene="5' UTR"
                     /label=5' UTR
     misc_feature    1141..1149
                     /label=KOZAK
     CDS             1146..1856
                     /codon_start=1
                     /gene="mKATE"
                     /label=mKate2
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             complement(1920..3681)
                     /gene="5' Viral freatures'"
                     /label=5' Viral freatures'
     CDS             complement(2057..2072)
                     /gene="cPPT"
                     /label=cPPT
     CDS             complement(2603..2836)
                     /gene="RRE"
                     /label=RRE
     CDS             complement(3346..3390)
                     /gene="Psi"
                     /label=Psi
     CDS             complement(3501..3681)
                     /gene="5'LTR"
                     /product="HIV-1_5_LTR"
                     /label=5'LTR
                     /note="HIV-1_5_LTR"
     CDS             complement(3701..4277)
                     /gene="CMVp"
                     /product="CMV_immearly_promoter"
                     /label=CMVp
                     /note="CMV_immearly_promoter"
     CDS             complement(3911..4198)
                     /gene="CAG_enhancer"
                     /label=CAG_enhancer
     CDS             4579..4607
                     /gene="Ampp"
                     /label=Ampp
     CDS             4649..5509
                     /codon_start=1
                     /gene="Amp"
                     /label=Amp
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(6718..6837)
                     /gene="SV40-pA"
                     /label=SV40-pA
     CDS             complement(6973..7344)
                     /codon_start=1
                     /gene="Bleo"
                     /label=Bleo
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     CDS             complement(7345..7412)
                     /gene="EM7p"
                     /label=EM7p
     CDS             complement(7506..7774)
                     /gene="SV40p"
                     /label=SV40p
     CDS             complement(7530..7607)
                     /gene="SV40-Ori"
                     /label=SV40-Ori
     CDS             7571..7786
                     /gene="SV40_enhancer"
                     /label=SV40_enhancer
     CDS             complement(8275..8502)
                     /gene="bGH-Ter"
                     /product="bGH_PA_terminator"
                     /label=bGH-Ter
                     /note="bGH_PA_terminator"
     CDS             complement(8531..8711)
                     /gene="del3'LTR"
                     /product="truncHIV-1_3_LTR"
                     /label=del3'LTR
                     /note="truncHIV-1_3_LTR"
     CDS             complement(9027..9048)
                     /gene="U3PPT"
                     /product="reverse transcriptase-associated RNase H cleavage
                     of the RNA at the polypurine tract (PPT), a short (ca. 11 
                     to 19 nucleotides [nt]) string of purines located at the 
                     upstream border of the 3' long terminal repeat "
                     /label=U3PPT
                     /note="reverse transcriptase-associated RNase H cleavage of
                     the RNA at the polypurine tract (PPT), a short (ca. 11 to 
                     19 nucleotides [nt]) string of purines located at the 
                     upstream border of the 3' long terminal repeat"
     CDS             complement(9033..9048)
                     /gene="cPPT"
                     /label=cPPT
ORIGIN
        1 ggaaaaccca cctcctcctc ctcttgtgct tctagccagg cacaatcagc attggtagct
       61 gctgtattgc tacttgtgat tgctccatgt ttttctaggt ctcgaggtcg acggtatcga
      121 tgcggggagg cggcccaaag ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga
      181 agaggccgca gagccggcag caggccgcgg gaaggaaggt ccgctggatt gagggccgaa
      241 gggacgtagc agaaggacgt cccgcgcaga atccaggtgg caacacaggc gagcagccaa
      301 ggaaaggacg atgatttccc cgacaacacc acggaattgt cagtgcccaa cagccgagcc
      361 cctgtccagc agcgggcaag gcaggcggcg atgagttccg ccgtggcaat agggaggggg
      421 aaagcgaaag tcccggaaag gagctgacag gtggtggcaa tgccccaacc agtgggggtt
      481 gcgtcagcaa acacagtgca caccacgcca cgttgcctga caacgggcca caactcctca
      541 taaagagaca gcaaccagga tttatacaag gaggagaaaa tgaaagccat acgggaagca
      601 atagcatgat acaaaggcat taaagcagcg tatccacata gcgtaaaagg agcaacatag
      661 ttaagaatac cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc
      721 ttgatatcgc ggccgccctg cacctgcagg ctgattggcc aaagcctgat tggccaaatc
      781 ctgattggcc aagacctgat tggccaaact ctgattggcc aagtctctga ttggccaagg
      841 agctgattgg ccaatccctg atggccaacg aattcctgcc ctactgacac tgcctgccgc
      901 tgattggcca aagcctgatt ggccaaatcc tgattggcca agacctgatt ggccaaactc
      961 tgattggcca agtcctgatt ggccaagagc tgattggcca atccctgatt ggccaacgaa
     1021 tatccgcggc gcgccagacg ctagcggggg gctataaaag ggggtggggg cgttcgtcct
     1081 cactctagat ctgcgatcta agtaagcttg gcattccggt actgttggta aaccagctcg
     1141 ccaccatggt gtctaagggc gaagagctga ttaaggagaa catgcacatg aagctgtaca
     1201 tggagggcac cgtgaacaac caccacttca agtgcacatc cgagggcgaa ggcaagccct
     1261 acgagggcac ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct
     1321 tcgacatcct ggctaccagc ttcatgtacg gcagcaaaac cttcatcaac cacacccagg
     1381 gcatccccga cttctttaag cagtccttcc ctgagggctt cacatgggag agagtcacca
     1441 catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc
     1501 tcatctacaa cgtcaagatc agaggggtga acttcccatc caacggccct gtgatgcaga
     1561 agaaaacact cggctgggag gcctccaccg agatgctgta ccccgctgac ggcggcctgg
     1621 aaggcagaag cgacatggcc ctgaagctcg tgggcggggg ccacctgatc tgcaacttga
     1681 agaccacata cagatccaag aaacccgcta agaacctcaa gatgcccggc gtctactatg
     1741 tggacagaag actggaaaga atcaaggagg ccgacaaaga gacctacgtc gagcagcacg
     1801 aggtggctgt ggccagatac tgcgacctcc ctagcaaact ggggcacaaa cttaattgat
     1861 aaaccggttg cagtcccggg taagcgggac tctggggttc gaaatgaccg accaagcatt
     1921 aaccaaactg gatctctgct gtccctgtaa taaacccgaa aattttgaat ttttgtaatt
     1981 tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct
     2041 gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac tgccatttgt
     2101 ctgcagaatt ggcgcacgca gtgccgatcc gttcactaat cgaatggatc tgtctctgtc
     2161 tctctctcca ccttcttctt ctattccttc gggcctgtcg ggtcccctcg gggttgggag
     2221 gtgggtctga aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac
     2281 agcaaaaact attcttaaac ctaccaagcc tcctactatc attatgaata attttatata
     2341 ccacagccaa tttgttatgt taaaccaatt ccacaaactt gcccatttat ctaattccaa
     2401 taattcttgt tcattctttt cttgctggtt ttgcgattct tcaattaagg agtgtattaa
     2461 gcttgtgtaa ttgttaattt ctctgtccca ctccatccag gtcgtgtgat tccaaatctg
     2521 ttccagagat ttattactcc aactagcatt ccaaggcaca gcagtggtgc aaatgagttt
     2581 tccagagcaa ccccaaatcc ccaggagctg ttgatccttt aggtatcttt ccacagccag
     2641 gattcttgcc tggagctgct tgatgcccca gactgtgagt tgcaacagat gctgttgcgc
     2701 ctcaatagcc ctcagcaaat tgttctgctg ctgcactata ccagacaata attgtctggc
     2761 ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt cctgctgctc ccaagaaccc
     2821 aaggaacaaa gctcctattc ccactgctct tttttctctc tgcaccactc ttctctttgc
     2881 cttggtgggt gctactccta atggttcaat ttttactact ttatatttat ataattcact
     2941 tctccaattg tccctcatat ctcctcctcc aggtctgaag atcagcggcc gcttgctgtg
     3001 cggtggtctt acttttgttt tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt
     3061 ttatctctat cctttgatgc acacaataga gggttgctac tgtattatat aatgatctaa
     3121 gttcttctga tcctgtctga agggatggtt gtagctgtcc cagtatttgt ctacagcctt
     3181 ctgatgtttc taacaggcca ggattaactg cgaatcgttc tagctccctg cttgcccata
     3241 ctatatgttt taatttatat tttttctttc cccctggcct taaccgaatt ttttcccatc
     3301 gcgatctaat tctcccccgc ttaatactga cgctctcgca cccatctctc tccttctagc
     3361 ctccgctagt caaaattttt ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc
     3421 gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc tggtttccct ttcgctttca
     3481 agtccctgtt cgggcgccac tgctagagat tttccacact gactaaaagg gtctgaggga
     3541 tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa
     3601 gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat
     3661 ctggtctaac cagagagacc cagtacaggc aaaacgcgct gcttatatag acctcccacc
     3721 gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt
     3781 cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa
     3841 atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca
     3901 tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca
     3961 tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac
     4021 ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc
     4081 attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg
     4141 tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa
     4201 cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac tattaataac
     4261 tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac atcgcgaagc agcgcaaaac
     4321 gcctaaccct aagcagattc ttcatgcaat tgtcggtcaa gccttgcctt gttgtagctt
     4381 aaattttgct cgcgcactac tcagcgacct ccaacacaca agcagggagc agatactggc
     4441 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatagggga tcgggagatc
     4501 tcccgatccg tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4561 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4621 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     4681 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     4741 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     4801 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     4861 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     4921 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     4981 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     5041 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     5101 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     5161 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     5221 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     5281 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     5341 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     5401 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     5461 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     5521 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     5581 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     5641 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5701 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5761 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5821 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5881 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5941 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     6001 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6061 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6121 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     6181 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     6241 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     6301 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     6361 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     6421 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     6481 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     6541 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     6601 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     6661 cagctatgac catgattacg ccaagctcta gctagaggtc gacggtatac agacatgata
     6721 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     6781 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     6841 ggggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc
     6901 ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg
     6961 tagcacgtgt cagtcctgct cctcggccac gaagtgcacg cagttgccgg ccgggtcgcg
     7021 cagggcgaac tcccgccccc acggctgctc gccgatctcg gtcatggccg gcccggaggc
     7081 gtcccggaag ttcgtggaca cgacctccga ccactcggcg tacagctcgt ccaggccgcg
     7141 cacccacacc caggccaggg tgttgtccgg caccacctgg tcctggaccg cgctgatgaa
     7201 cagggtcacg tcgtcccgga ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa
     7261 cccgagccgg tcggtccaga actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg
     7321 aacggcactg gtcaacttgg ccatggttta gttcctcacc ttgtcgtatt atactatgcc
     7381 gatatactat gccgatgatt aattgtcaac acgtgctgat cagatccgaa aatggatata
     7441 caagctcccg ggagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc
     7501 tggaatagct cagaggcaga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag
     7561 ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg
     7621 ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     7681 gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt
     7741 ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt ccacagaatt
     7801 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     7861 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     7921 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     7981 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     8041 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     8101 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     8161 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     8221 agggcgcgtg gggatacccc ctagagcccc agctggttct ttccgcctca gaagccatag
     8281 agcccaccgc atccccagca tgcctgctat tgtcttccca atcctccccc ttgctgtcct
     8341 gccccacccc accccccaga atagaatgac acctactcag acaatgcgat gcaatttcct
     8401 cattttatta ggaaaggaca gtgggagtgg caccttccag ggtcaaggaa ggcacggggg
     8461 aggggcaaac aacagatggc tggcaactag aaggcacagt cgaggctgat cagcgggttt
     8521 aaacgggccc tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta
     8581 ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga
     8641 ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac
     8701 cagagagacc cagtacagtc cggatgcagc tctcgggcca tgtgatgaaa tgctaggcgg
     8761 ctgtcaaacc tccactctaa tacttctctc tccgggtcat ccatcccatg caggctcaca
     8821 gggtgtaaca agcgggtgtt ctctccttca ttggcttctt ctaccttctc ttgctcaact
     8881 ggtactagct tgtagcacca tccaaaggtc agtggatatc tgatccctgg ccctggtgtg
     8941 tagttctgcc aatcagggaa gtagccttgt gtgtggtaga tccacagatc aaggatatct
     9001 tgtcttcgtt gggagtgaat tagcccttcc agtcccccct tttcttttaa aaagtggcta
     9061 agatctacag ctgccttgta agtcattggt cttaaaggta cctgaggtgt gact
//
