LOCUS       Exported                9112 bp ds-DNA     circular SYN 02-JUN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    SnapGene DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 2, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     GenBank                 9112 bp    DNA     circular SYN 29-MAY-2017
            ACCESSION   pLN352
            KEYWORDS:   .
FEATURES             Location/Qualifiers
     source          1..9112
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(121..708)
                     /gene="WPRE"
                     /product="The WPRE seems to increase the expression of the 
                     marker gene when introduced in its 3' untranslated region. 
                     This element is believed to exert some posttranslational 
                     regulation. "
                     /label=WPRE
                     /note="The WPRE seems to increase the expression of the 
                     marker gene when introduced in its 3' untranslated region. 
                     This element is believed to exert some posttranslational 
                     regulation."
     misc_signal     751..765
                     /label=Elk-1 (1)
                     /note="http://motifmap.ics.uci.edu/"
     misc_signal     769..780
                     /label=Elk-1 (2)
                     /note="http://motifmap.ics.uci.edu/"
     misc_signal     771..781
                     /label=Elk-1 (3)
                     /note="http://motifmap.ics.uci.edu/"
     misc_signal     784..794
                     /label=Elk-1 (3)
                     /note="http://motifmap.ics.uci.edu/"
     misc_signal     798..808
                     /label=Elk-1 (3.1)
                     /note="http://motifmap.ics.uci.edu/"
     misc_signal     812..822
                     /label=Elk-1 (3.1)
                     /note="http://motifmap.ics.uci.edu/"
     misc_signal     826..836
                     /label=Elk-1 (3.2)
                     /note="http://motifmap.ics.uci.edu/"
     misc_signal     840..854
                     /label=Elk-1 (1)
                     /note="http://motifmap.ics.uci.edu/"
     misc_signal     858..869
                     /label=Elk-1 (2)
                     /note="http://motifmap.ics.uci.edu/"
     misc_signal     878..897
                     /label=20BP LINKER
     misc_signal     901..911
                     /label=Elk-1 (3.1)
                     /note="http://motifmap.ics.uci.edu/"
     misc_signal     915..925
                     /label=Elk-1 (3)
                     /note="http://motifmap.ics.uci.edu/"
     misc_signal     929..939
                     /label=Elk-1 (3.2)
                     /note="http://motifmap.ics.uci.edu/"
     misc_signal     943..957
                     /label=Elk-1 (1)
                     /note="http://motifmap.ics.uci.edu/"
     misc_signal     961..972
                     /label=Elk-1 (2)
                     /note="http://motifmap.ics.uci.edu/"
     misc_signal     976..986
                     /label=Elk-1 (3)
                     /note="http://motifmap.ics.uci.edu/"
     misc_signal     990..1000
                     /label=Elk-1 (3.1)
                     /note="http://motifmap.ics.uci.edu/"
     misc_signal     1004..1014
                     /label=Elk-1 (3.2)
                     /note="http://motifmap.ics.uci.edu/"
     misc_signal     1016..1025
                     /label=10bp spacer
     CDS             1034..1084
                     /gene="lateADEp"
                     /product="major late promoter of adenovirus"
                     /label=lateADEp
                     /note="major late promoter of adenovirus"
     misc_feature    1085..1130
                     /gene="5' UTR"
                     /label=5' UTR
     misc_feature    1139..1147
                     /label=KOZAK
     CDS             1144..1854
                     /codon_start=1
                     /gene="mKATE"
                     /label=mKate2
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             complement(1918..3679)
                     /gene="5' Viral freatures'"
                     /label=5' Viral freatures'
     CDS             complement(2055..2070)
                     /gene="cPPT"
                     /label=cPPT
     CDS             complement(2601..2834)
                     /gene="RRE"
                     /label=RRE
     CDS             complement(3344..3388)
                     /gene="Psi"
                     /label=Psi
     CDS             complement(3499..3679)
                     /gene="5'LTR"
                     /product="HIV-1_5_LTR"
                     /label=5'LTR
                     /note="HIV-1_5_LTR"
     CDS             complement(3699..4275)
                     /gene="CMVp"
                     /product="CMV_immearly_promoter"
                     /label=CMVp
                     /note="CMV_immearly_promoter"
     CDS             complement(3909..4196)
                     /gene="CAG_enhancer"
                     /label=CAG_enhancer
     CDS             4577..4605
                     /gene="Ampp"
                     /label=Ampp
     CDS             4647..5507
                     /codon_start=1
                     /gene="Amp"
                     /label=Amp
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(6716..6835)
                     /gene="SV40-pA"
                     /label=SV40-pA
     CDS             complement(6971..7342)
                     /codon_start=1
                     /gene="Bleo"
                     /label=Bleo
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     CDS             complement(7343..7410)
                     /gene="EM7p"
                     /label=EM7p
     CDS             complement(7504..7772)
                     /gene="SV40p"
                     /label=SV40p
     CDS             complement(7528..7605)
                     /gene="SV40-Ori"
                     /label=SV40-Ori
     CDS             7569..7784
                     /gene="SV40_enhancer"
                     /label=SV40_enhancer
     CDS             complement(8273..8500)
                     /gene="bGH-Ter"
                     /product="bGH_PA_terminator"
                     /label=bGH-Ter
                     /note="bGH_PA_terminator"
     CDS             complement(8529..8709)
                     /gene="del3'LTR"
                     /product="truncHIV-1_3_LTR"
                     /label=del3'LTR
                     /note="truncHIV-1_3_LTR"
     CDS             complement(9025..9046)
                     /gene="U3PPT"
                     /product="reverse transcriptase-associated RNase H cleavage
                     of the RNA at the polypurine tract (PPT), a short (ca. 11 
                     to 19 nucleotides [nt]) string of purines located at the 
                     upstream border of the 3' long terminal repeat "
                     /label=U3PPT
                     /note="reverse transcriptase-associated RNase H cleavage of
                     the RNA at the polypurine tract (PPT), a short (ca. 11 to 
                     19 nucleotides [nt]) string of purines located at the 
                     upstream border of the 3' long terminal repeat"
     CDS             complement(9031..9046)
                     /gene="cPPT"
                     /label=cPPT
ORIGIN
        1 ggaaaaccca cctcctcctc ctcttgtgct tctagccagg cacaatcagc attggtagct
       61 gctgtattgc tacttgtgat tgctccatgt ttttctaggt ctcgaggtcg acggtatcga
      121 tgcggggagg cggcccaaag ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga
      181 agaggccgca gagccggcag caggccgcgg gaaggaaggt ccgctggatt gagggccgaa
      241 gggacgtagc agaaggacgt cccgcgcaga atccaggtgg caacacaggc gagcagccaa
      301 ggaaaggacg atgatttccc cgacaacacc acggaattgt cagtgcccaa cagccgagcc
      361 cctgtccagc agcgggcaag gcaggcggcg atgagttccg ccgtggcaat agggaggggg
      421 aaagcgaaag tcccggaaag gagctgacag gtggtggcaa tgccccaacc agtgggggtt
      481 gcgtcagcaa acacagtgca caccacgcca cgttgcctga caacgggcca caactcctca
      541 taaagagaca gcaaccagga tttatacaag gaggagaaaa tgaaagccat acgggaagca
      601 atagcatgat acaaaggcat taaagcagcg tatccacata gcgtaaaagg agcaacatag
      661 ttaagaatac cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc
      721 ttgatatcgc ggccgccctg cacctgcagg aaacaggaag ttcgtagccc aaccggaagt
      781 atcaaccgga agtagacgac cggaagtaac tgaccggaag tagtcgaccg gaagtggaga
      841 aacaggaagt tcgttcccca accggaagtc gaattcctgc cctactgaca ctgcctgccg
      901 gaccggaagt aagcaaccgg aagtaatcga ccggaagtgg acaaacagga agttcgtact
      961 ccaaccggaa gtgtcaaccg gaagtagagg accggaagta tccgaccgga agtgcgaata
     1021 tccgcggcgc gccagacgct agcggggggc tataaaaggg ggtgggggcg ttcgtcctca
     1081 ctctagatct gcgatctaag taagcttggc attccggtac tgttggtaaa ccagctcgcc
     1141 accatggtgt ctaagggcga agagctgatt aaggagaaca tgcacatgaa gctgtacatg
     1201 gagggcaccg tgaacaacca ccacttcaag tgcacatccg agggcgaagg caagccctac
     1261 gagggcaccc agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc
     1321 gacatcctgg ctaccagctt catgtacggc agcaaaacct tcatcaacca cacccagggc
     1381 atccccgact tctttaagca gtccttccct gagggcttca catgggagag agtcaccaca
     1441 tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc
     1501 atctacaacg tcaagatcag aggggtgaac ttcccatcca acggccctgt gatgcagaag
     1561 aaaacactcg gctgggaggc ctccaccgag atgctgtacc ccgctgacgg cggcctggaa
     1621 ggcagaagcg acatggccct gaagctcgtg ggcgggggcc acctgatctg caacttgaag
     1681 accacataca gatccaagaa acccgctaag aacctcaaga tgcccggcgt ctactatgtg
     1741 gacagaagac tggaaagaat caaggaggcc gacaaagaga cctacgtcga gcagcacgag
     1801 gtggctgtgg ccagatactg cgacctccct agcaaactgg ggcacaaact taattgataa
     1861 accggttgca gtcccgggta agcgggactc tggggttcga aatgaccgac caagcattaa
     1921 ccaaactgga tctctgctgt ccctgtaata aacccgaaaa ttttgaattt ttgtaatttg
     1981 tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc tttcccctgc
     2041 actgtacccc ccaatccccc cttttctttt aaaattgtgg atgaatactg ccatttgtct
     2101 gcagaattgg cgcacgcagt gccgatccgt tcactaatcg aatggatctg tctctgtctc
     2161 tctctccacc ttcttcttct attccttcgg gcctgtcggg tcccctcggg gttgggaggt
     2221 gggtctgaaa cgataatggt gaatatccct gcctaactct attcactata gaaagtacag
     2281 caaaaactat tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc
     2341 acagccaatt tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata
     2401 attcttgttc attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc
     2461 ttgtgtaatt gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt
     2521 ccagagattt attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc
     2581 cagagcaacc ccaaatcccc aggagctgtt gatcctttag gtatctttcc acagccagga
     2641 ttcttgcctg gagctgcttg atgccccaga ctgtgagttg caacagatgc tgttgcgcct
     2701 caatagccct cagcaaattg ttctgctgct gcactatacc agacaataat tgtctggcct
     2761 gtaccgtcag cgtcattgac gctgcgccca tagtgcttcc tgctgctccc aagaacccaa
     2821 ggaacaaagc tcctattccc actgctcttt tttctctctg caccactctt ctctttgcct
     2881 tggtgggtgc tactcctaat ggttcaattt ttactacttt atatttatat aattcacttc
     2941 tccaattgtc cctcatatct cctcctccag gtctgaagat cagcggccgc ttgctgtgcg
     3001 gtggtcttac ttttgttttg ctcttcctct atcttgtcta aagcttcctt ggtgtctttt
     3061 atctctatcc tttgatgcac acaatagagg gttgctactg tattatataa tgatctaagt
     3121 tcttctgatc ctgtctgaag ggatggttgt agctgtccca gtatttgtct acagccttct
     3181 gatgtttcta acaggccagg attaactgcg aatcgttcta gctccctgct tgcccatact
     3241 atatgtttta atttatattt tttctttccc cctggcctta accgaatttt ttcccatcgc
     3301 gatctaattc tcccccgctt aatactgacg ctctcgcacc catctctctc cttctagcct
     3361 ccgctagtca aaatttttgg cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc
     3421 gcttcagcaa gccgagtcct gcgtcgagag agctcctctg gtttcccttt cgctttcaag
     3481 tccctgttcg ggcgccactg ctagagattt tccacactga ctaaaagggt ctgagggatc
     3541 tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc
     3601 tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct
     3661 ggtctaacca gagagaccca gtacaggcaa aacgcgctgc ttatatagac ctcccaccgt
     3721 acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc
     3781 cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat
     3841 ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg
     3901 gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg
     3961 tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt
     4021 ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat
     4081 tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc
     4141 aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg
     4201 cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta
     4261 gtcaataatc aatgtcaacg cgtatatctg gcccgtacat cgcgaagcag cgcaaaacgc
     4321 ctaaccctaa gcagattctt catgcaattg tcggtcaagc cttgccttgt tgtagcttaa
     4381 attttgctcg cgcactactc agcgacctcc aacacacaag cagggagcag atactggctt
     4441 aactatgcgg catcagagca gattgtactg agagtgcacc ataggggatc gggagatctc
     4501 ccgatccgtc gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4561 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4621 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4681 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     4741 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     4801 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     4861 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     4921 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     4981 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5041 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5101 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     5161 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     5221 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5281 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     5341 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     5401 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     5461 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     5521 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     5581 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     5641 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5701 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5761 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5821 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5881 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5941 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6001 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6061 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6121 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6181 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6241 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6301 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6361 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     6421 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     6481 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     6541 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     6601 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     6661 gctatgacca tgattacgcc aagctctagc tagaggtcga cggtatacag acatgataag
     6721 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     6781 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttgg
     6841 ggtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc
     6901 ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgta
     6961 gcacgtgtca gtcctgctcc tcggccacga agtgcacgca gttgccggcc gggtcgcgca
     7021 gggcgaactc ccgcccccac ggctgctcgc cgatctcggt catggccggc ccggaggcgt
     7081 cccggaagtt cgtggacacg acctccgacc actcggcgta cagctcgtcc aggccgcgca
     7141 cccacaccca ggccagggtg ttgtccggca ccacctggtc ctggaccgcg ctgatgaaca
     7201 gggtcacgtc gtcccggacc acaccggcga agtcgtcctc cacgaagtcc cgggagaacc
     7261 cgagccggtc ggtccagaac tcgaccgctc cggcgacgtc gcgcgcggtg agcaccggaa
     7321 cggcactggt caacttggcc atggtttagt tcctcacctt gtcgtattat actatgccga
     7381 tatactatgc cgatgattaa ttgtcaacac gtgctgatca gatccgaaaa tggatataca
     7441 agctcccggg agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
     7501 gaatagctca gaggcagagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     7561 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     7621 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     7681 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
     7741 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagaattaa
     7801 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     7861 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     7921 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     7981 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     8041 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     8101 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     8161 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     8221 ggcgcgtggg gataccccct agagccccag ctggttcttt ccgcctcaga agccatagag
     8281 cccaccgcat ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc
     8341 cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca
     8401 ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag
     8461 gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca gcgggtttaa
     8521 acgggccctg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc
     8581 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg
     8641 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
     8701 gagagaccca gtacagtccg gatgcagctc tcgggccatg tgatgaaatg ctaggcggct
     8761 gtcaaacctc cactctaata cttctctctc cgggtcatcc atcccatgca ggctcacagg
     8821 gtgtaacaag cgggtgttct ctccttcatt ggcttcttct accttctctt gctcaactgg
     8881 tactagcttg tagcaccatc caaaggtcag tggatatctg atccctggcc ctggtgtgta
     8941 gttctgccaa tcagggaagt agccttgtgt gtggtagatc cacagatcaa ggatatcttg
     9001 tcttcgttgg gagtgaatta gcccttccag tccccccttt tcttttaaaa agtggctaag
     9061 atctacagct gccttgtaag tcattggtct taaaggtacc tgaggtgtga ct
//
