LOCUS       Exported                8974 bp ds-DNA     circular SYN 02-JUN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    SnapGene DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 2, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     GenBank                 8974 bp    DNA     circular SYN 29-MAY-2017
            ACCESSION   pLN431
            KEYWORDS:   .
FEATURES             Location/Qualifiers
     source          1..8974
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             493..508
                     /gene="cPPT"
                     /label=cPPT
     CDS             655..943
                     /gene="G8p"
                     /label=G8p
     CDS             678..693
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             697..712
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             721..736
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             740..755
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             764..779
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             792..807
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             811..826
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             835..850
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             857..897
                     /gene="lateADEp"
                     /product="major late promoter of adenovirus"
                     /label=lateADEp
                     /note="major late promoter of adenovirus"
     CDS             944..952
                     /gene="KOZAK"
                     /label=KOZAK
     CDS             950..1660
                     /codon_start=1
                     /gene="mKate2"
                     /label=mKate2
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             1664..1691
                     /gene="BS(Pe)"
                     /label=BS(Pe)
     CDS             1717..2304
                     /gene="WPRE"
                     /product="The WPRE seems to increase the expression of the 
                     marker gene when introduced in its 3' untranslated region. 
                     This element is believed to exert some posttranslational 
                     regulation. "
                     /label=WPRE
                     /note="The WPRE seems to increase the expression of the 
                     marker gene when introduced in its 3' untranslated region. 
                     This element is believed to exert some posttranslational 
                     regulation."
     CDS             2491..2512
                     /gene="U3PPT"
                     /product="reverse transcriptase-associated RNase H cleavage
                     of the RNA at the polypurine tract (PPT), a short (ca. 11 
                     to 19 nucleotides [nt]) string of purines located at the 
                     upstream border of the 3' long terminal repeat "
                     /label=U3PPT
                     /note="reverse transcriptase-associated RNase H cleavage of
                     the RNA at the polypurine tract (PPT), a short (ca. 11 to 
                     19 nucleotides [nt]) string of purines located at the 
                     upstream border of the 3' long terminal repeat"
     CDS             2491..2506
                     /gene="cPPT"
                     /label=cPPT
     CDS             2828..3008
                     /gene="del3'LTR"
                     /product="truncHIV-1_3_LTR"
                     /label=del3'LTR
                     /note="truncHIV-1_3_LTR"
     CDS             3037..3264
                     /gene="bGH-Ter"
                     /product="bGH_PA_terminator"
                     /label=bGH-Ter
                     /note="bGH_PA_terminator"
     CDS             complement(3753..3968)
                     /gene="SV40_enhancer"
                     /label=SV40_enhancer
     CDS             3765..4033
                     /gene="SV40p"
                     /label=SV40p
     CDS             3932..4009
                     /gene="SV40-Ori"
                     /label=SV40-Ori
     CDS             4127..4194
                     /gene="EM7p"
                     /label=EM7p
     CDS             4195..4566
                     /codon_start=1
                     /gene="Bleo"
                     /label=Bleo
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     CDS             4702..4821
                     /gene="SV40-pA"
                     /label=SV40-pA
     CDS             complement(6030..6890)
                     /codon_start=1
                     /gene="Amp"
                     /label=Amp
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(6932..6960)
                     /gene="Ampp"
                     /label=Ampp
     CDS             7262..7838
                     /gene="CMVp"
                     /product="CMV_immearly_promoter"
                     /label=CMVp
                     /note="CMV_immearly_promoter"
     CDS             7341..7628
                     /gene="CAG_enhancer"
                     /label=CAG_enhancer
     CDS             7858..645
                     /gene="5' Viral freatures'"
                     /label=5' Viral freatures'
     CDS             7858..8038
                     /gene="5'LTR"
                     /product="HIV-1_5_LTR"
                     /label=5'LTR
                     /note="HIV-1_5_LTR"
     CDS             8149..8193
                     /gene="Psi"
                     /label=Psi
     CDS             8703..8936
                     /gene="RRE"
                     /label=RRE
ORIGIN
        1 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
       61 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
      121 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
      181 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
      241 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
      301 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
      361 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
      421 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
      481 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      541 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      601 aattttcggg tttattacag ggacagcaga gatccagttt ggttaatgga tcctcgatag
      661 gtaccgagtt tctagacgga gtactgtcct ccgagcggag tactgtcctc cgactcgagc
      721 ggagtactgt cctccgatcg gagtactgtc ctccgcgaat tccggagtac tgtcctccga
      781 agacgctaga cggagtactg tcctccgagc ggagtactgt cctccgactc gagcggagta
      841 ctgtcctccg gctagcgggg ggctataaaa gggggtgggg gcgttcgtcc tcactctaga
      901 tctgcgatct aagtaagctt ggcattccgg tactgttggt aaagccacca tggtgtctaa
      961 gggcgaagag ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgaa
     1021 caaccaccac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac
     1081 catgagaatc aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac
     1141 cagcttcatg tacggcagca aaaccttcat caaccacacc cagggcatcc ccgacttctt
     1201 taagcagtcc ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg
     1261 cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa
     1321 gatcagaggg gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cactcggctg
     1381 ggaggcctcc accgagatgc tgtaccccgc tgacggcggc ctggaaggca gaagcgacat
     1441 ggccctgaag ctcgtgggcg ggggccacct gatctgcaac ttgaagacca catacagatc
     1501 caagaaaccc gctaagaacc tcaagatgcc cggcgtctac tatgtggaca gaagactgga
     1561 aagaatcaag gaggccgaca aagagaccta cgtcgagcag cacgaggtgg ctgtggccag
     1621 atactgcgac ctccctagca aactggggca caaacttaat taggcaccgc ttgaagtctt
     1681 taattaaata ccggtcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     1741 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     1801 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     1861 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     1921 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     1981 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     2041 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     2101 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     2161 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     2221 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     2281 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg
     2341 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     2401 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     2461 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     2521 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     2581 ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga cctttggatg
     2641 gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa
     2701 cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt
     2761 agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga
     2821 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     2881 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     2941 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     3001 ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     3061 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     3121 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     3181 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     3241 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     3301 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     3361 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     3421 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     3481 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     3541 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     3601 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     3661 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     3721 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     3781 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     3841 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3901 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3961 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     4021 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     4081 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     4141 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
     4201 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     4261 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     4321 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     4381 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     4441 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     4501 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     4561 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     4621 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     4681 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     4741 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     4801 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     4861 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     4921 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     4981 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     5041 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     5101 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     5161 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     5221 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5281 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5341 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5401 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5461 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5521 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5581 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5641 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5701 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     5761 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     5821 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     5881 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     5941 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     6001 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6061 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6121 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     6181 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     6241 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     6301 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     6361 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     6421 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6481 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     6541 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6601 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     6661 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     6721 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     6781 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     6841 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     6901 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     6961 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7021 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     7081 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     7141 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     7201 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     7261 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     7321 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     7381 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     7441 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     7501 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     7561 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     7621 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     7681 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     7741 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     7801 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
     7861 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7921 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7981 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     8041 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     8101 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     8161 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     8221 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     8281 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     8341 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     8401 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     8461 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     8521 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     8581 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     8641 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     8701 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     8761 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     8821 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     8881 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     8941 atttggggtt gctctggaaa actcatttgc acca
//
