LOCUS       Exported                9077 bp ds-DNA     circular SYN 02-JUN-2017
DEFINITION  FuGW-G14p-mKate2.
ACCESSION   .
VERSION     .
KEYWORDS    SnapGene DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 2, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     GenBank                 9077 bp    DNA     circular SYN 29-MAY-2017
            ACCESSION   pLN432
            KEYWORDS:   .
FEATURES             Location/Qualifiers
     source          1..9077
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             499..514
                     /gene="cPPT"
                     /label=cPPT
     CDS             683..785
                     /gene="5xGAL4-BS"
                     /label=5xGAL4-BS
     CDS             684..699
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             703..718
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             727..742
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             746..761
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             770..785
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             798..813
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             817..832
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             841..856
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             859..874
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             878..893
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             902..917
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             925..940
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             944..959
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             968..983
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             983..1029
                     /codon_start=2
                     /gene="lateADEp"
                     /product="major late promoter of adenovirus"
                     /label=lateADEp
                     /note="major late promoter of adenovirus"
                     /translation="LAGGYKRGWGRSSSL"
     misc_feature    1077..1085
                     /label=KOZAK
     CDS             1082..1792
                     /codon_start=1
                     /gene="mKATE"
                     /label=mKate2
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             1826..2413
                     /gene="WPRE"
                     /product="The WPRE seems to increase the expression of the 
                     marker gene when introduced in its 3' untranslated region. 
                     This element is believed to exert some posttranslational 
                     regulation. "
                     /label=WPRE
                     /note="The WPRE seems to increase the expression of the 
                     marker gene when introduced in its 3' untranslated region. 
                     This element is believed to exert some posttranslational 
                     regulation."
     CDS             2600..2621
                     /gene="U3PPT"
                     /product="reverse transcriptase-associated RNase H cleavage
                     of the RNA at the polypurine tract (PPT), a short (ca. 11 
                     to 19 nucleotides [nt]) string of purines located at the 
                     upstream border of the 3' long terminal repeat "
                     /label=U3PPT
                     /note="reverse transcriptase-associated RNase H cleavage of
                     the RNA at the polypurine tract (PPT), a short (ca. 11 to 
                     19 nucleotides [nt]) string of purines located at the 
                     upstream border of the 3' long terminal repeat"
     CDS             2600..2615
                     /gene="cPPT"
                     /label=cPPT
     CDS             2937..3117
                     /gene="del3'LTR"
                     /product="truncHIV-1_3_LTR"
                     /label=del3'LTR
                     /note="truncHIV-1_3_LTR"
     CDS             3146..3373
                     /gene="bGH-Ter"
                     /product="bGH_PA_terminator"
                     /label=bGH-Ter
                     /note="bGH_PA_terminator"
     CDS             complement(3862..4077)
                     /gene="SV40_enhancer"
                     /label=SV40_enhancer
     CDS             3874..4142
                     /gene="SV40p"
                     /label=SV40p
     CDS             4041..4118
                     /gene="SV40-Ori"
                     /label=SV40-Ori
     CDS             4236..4303
                     /gene="EM7p"
                     /label=EM7p
     CDS             4304..4675
                     /codon_start=1
                     /gene="Bleo"
                     /label=Bleo
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     CDS             4811..4930
                     /gene="SV40-pA"
                     /label=SV40-pA
     CDS             complement(6139..6999)
                     /codon_start=1
                     /gene="Amp"
                     /label=Amp
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(7041..7069)
                     /gene="Ampp"
                     /label=Ampp
     CDS             7371..7947
                     /gene="CMVp"
                     /product="CMV_immearly_promoter"
                     /label=CMVp
                     /note="CMV_immearly_promoter"
     CDS             7450..7737
                     /gene="CAG_enhancer"
                     /label=CAG_enhancer
     CDS             7967..651
                     /gene="5' Viral freatures'"
                     /label=5' Viral freatures'
     CDS             7967..8147
                     /gene="5'LTR"
                     /product="HIV-1_5_LTR"
                     /label=5'LTR
                     /note="HIV-1_5_LTR"
     CDS             8258..8302
                     /gene="Psi"
                     /label=Psi
     CDS             8812..9045
                     /gene="RRE"
                     /label=RRE
ORIGIN
        1 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
       61 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
      121 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
      181 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
      241 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
      301 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
      361 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
      421 gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca
      481 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
      541 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
      601 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt aatggatcct
      661 cgataggtac cgagtttcta gacggagtac tgtcctccga gcggagtact gtcctccgac
      721 tcgagcggag tactgtcctc cgatcggagt actgtcctcc gcgaattccg gagtactgtc
      781 ctccgaagac gctagacgga gtactgtcct ccgagcggag tactgtcctc cgactcgagc
      841 ggagtactgt cctccggcgg agtactgtcc tccgagcgga gtactgtcct ccgactcgag
      901 cggagtactg tcctccggct agacggagta ctgtcctccg agcggagtac tgtcctccga
      961 ctcgagcgga gtactgtcct ccgctagcgg ggggctataa aagggggtgg gggcgttcgt
     1021 cctcactcta gatctgcgat ctaagtaagc ttggcattcc ggtactgttg gtaaagccac
     1081 catggtgtct aagggcgaag agctgattaa ggagaacatg cacatgaagc tgtacatgga
     1141 gggcaccgtg aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga
     1201 gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga
     1261 catcctggct accagcttca tgtacggcag caaaaccttc atcaaccaca cccagggcat
     1321 ccccgacttc tttaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata
     1381 cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat
     1441 ctacaacgtc aagatcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa
     1501 aacactcggc tgggaggcct ccaccgagat gctgtacccc gctgacggcg gcctggaagg
     1561 cagaagcgac atggccctga agctcgtggg cgggggccac ctgatctgca acttgaagac
     1621 cacatacaga tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actatgtgga
     1681 cagaagactg gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgaggt
     1741 ggctgtggcc agatactgcg acctccctag caaactgggg cacaaactta attgataaac
     1801 cggtcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     1861 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     1921 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     1981 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     2041 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     2101 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     2161 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     2221 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     2281 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     2341 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     2401 ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa
     2461 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     2521 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     2581 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     2641 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
     2701 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc
     2761 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
     2821 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
     2881 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
     2941 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     3001 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     3061 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
     3121 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     3181 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     3241 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     3301 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     3361 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     3421 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3481 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     3541 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     3601 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     3661 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     3721 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     3781 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     3841 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     3901 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     3961 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     4021 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4081 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     4141 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     4201 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     4261 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
     4321 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     4381 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     4441 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     4501 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     4561 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     4621 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
     4681 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     4741 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     4801 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4861 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4921 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     4981 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     5041 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     5101 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     5161 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     5221 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     5281 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     5341 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     5401 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     5461 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     5521 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     5581 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     5641 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     5701 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     5761 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     5821 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     5881 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     5941 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6001 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     6061 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     6121 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     6181 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     6241 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     6301 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     6361 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6421 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     6481 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     6541 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     6601 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     6661 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     6721 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     6781 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     6841 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6901 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6961 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     7021 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     7081 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     7141 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
     7201 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
     7261 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
     7321 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
     7381 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     7441 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     7501 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     7561 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     7621 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     7681 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     7741 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     7801 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     7861 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     7921 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt
     7981 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8041 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8101 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     8161 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     8221 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     8281 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     8341 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     8401 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     8461 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     8521 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     8581 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     8641 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     8701 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     8761 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     8821 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     8881 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     8941 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     9001 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     9061 ctctggaaaa ctcattt
//
