LOCUS       Exported                9100 bp ds-DNA     circular SYN 02-JUN-2017
DEFINITION  FuGW-G14p-mKate2-1xBS(Pe).
ACCESSION   .
VERSION     .
KEYWORDS    SnapGene DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 2, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     GenBank                 9100 bp    DNA     circular SYN 29-MAY-2017
            ACCESSION   pLN434
            KEYWORDS:   .
FEATURES             Location/Qualifiers
     source          1..9100
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             239..815
                     /gene="CMVp"
                     /product="CMV_immearly_promoter"
                     /label=CMVp
                     /note="CMV_immearly_promoter"
     CDS             318..605
                     /gene="CAG_enhancer"
                     /label=CAG_enhancer
     CDS             835..2596
                     /gene="5' Viral freatures'"
                     /label=5' Viral freatures'
     CDS             835..1015
                     /gene="5'LTR"
                     /product="HIV-1_5_LTR"
                     /label=5'LTR
                     /note="HIV-1_5_LTR"
     CDS             1126..1170
                     /gene="Psi"
                     /label=Psi
     CDS             1680..1913
                     /gene="RRE"
                     /label=RRE
     CDS             2444..2459
                     /gene="cPPT"
                     /label=cPPT
     CDS             2629..2644
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             2648..2663
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             2672..2687
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             2691..2706
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             2715..2730
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             2743..2758
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             2762..2777
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             2786..2801
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             2804..2819
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             2823..2838
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             2847..2862
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             2870..2885
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             2889..2904
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             2913..2928
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             2928..2974
                     /codon_start=2
                     /gene="lateADEp'"
                     /product="major late promoter of adenovirus"
                     /label=lateADEp'
                     /note="major late promoter of adenovirus"
                     /translation="LAGGYKRGWGRSSSL"
     misc_feature    3022..3030
                     /label=KOZAK
     CDS             3027..3737
                     /codon_start=1
                     /gene="mKate2"
                     /label=mKate2
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             3741..3768
                     /gene="BS(Pe)"
                     /label=BS(Pe)
     CDS             3794..4381
                     /gene="WPRE"
                     /product="The WPRE seems to increase the expression of the 
                     marker gene when introduced in its 3' untranslated region. 
                     This element is believed to exert some posttranslational 
                     regulation. "
                     /label=WPRE
                     /note="The WPRE seems to increase the expression of the 
                     marker gene when introduced in its 3' untranslated region. 
                     This element is believed to exert some posttranslational 
                     regulation."
     CDS             4568..4589
                     /gene="U3PPT"
                     /product="reverse transcriptase-associated RNase H cleavage
                     of the RNA at the polypurine tract (PPT), a short (ca. 11 
                     to 19 nucleotides [nt]) string of purines located at the 
                     upstream border of the 3' long terminal repeat "
                     /label=U3PPT
                     /note="reverse transcriptase-associated RNase H cleavage of
                     the RNA at the polypurine tract (PPT), a short (ca. 11 to 
                     19 nucleotides [nt]) string of purines located at the 
                     upstream border of the 3' long terminal repeat"
     CDS             4568..4583
                     /gene="cPPT"
                     /label=cPPT
     CDS             4905..5085
                     /gene="del3'LTR"
                     /product="truncHIV-1_3_LTR"
                     /label=del3'LTR
                     /note="truncHIV-1_3_LTR"
     CDS             5114..5341
                     /gene="bGH-Ter"
                     /product="bGH_PA_terminator"
                     /label=bGH-Ter
                     /note="bGH_PA_terminator"
     CDS             complement(5830..6045)
                     /gene="SV40_enhancer"
                     /label=SV40_enhancer
     CDS             5842..6110
                     /gene="SV40p"
                     /label=SV40p
     CDS             6009..6086
                     /gene="SV40-Ori"
                     /label=SV40-Ori
     CDS             6204..6271
                     /gene="EM7p"
                     /label=EM7p
     CDS             6272..6643
                     /codon_start=1
                     /gene="Bleo"
                     /label=Bleo
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     CDS             6779..6898
                     /gene="SV40-pA"
                     /label=SV40-pA
     CDS             complement(8107..8967)
                     /codon_start=1
                     /gene="Amp"
                     /label=Amp
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(9009..9037)
                     /gene="Ampp"
                     /label=Ampp
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatgg atcctcgata ggtaccgagt ttctagacgg agtactgtcc
     2641 tccgagcgga gtactgtcct ccgactcgag cggagtactg tcctccgatc ggagtactgt
     2701 cctccgcgaa ttccggagta ctgtcctccg aagacgctag acggagtact gtcctccgag
     2761 cggagtactg tcctccgact cgagcggagt actgtcctcc ggcggagtac tgtcctccga
     2821 gcggagtact gtcctccgac tcgagcggag tactgtcctc cggctagacg gagtactgtc
     2881 ctccgagcgg agtactgtcc tccgactcga gcggagtact gtcctccgct agcggggggc
     2941 tataaaaggg ggtgggggcg ttcgtcctca ctctagatct gcgatctaag taagcttggc
     3001 attccggtac tgttggtaaa gccaccatgg tgtctaaggg cgaagagctg attaaggaga
     3061 acatgcacat gaagctgtac atggagggca ccgtgaacaa ccaccacttc aagtgcacat
     3121 ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg
     3181 gcggccctct ccccttcgcc ttcgacatcc tggctaccag cttcatgtac ggcagcaaaa
     3241 ccttcatcaa ccacacccag ggcatccccg acttctttaa gcagtccttc cctgagggct
     3301 tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca
     3361 ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcccat
     3421 ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggcctccacc gagatgctgt
     3481 accccgctga cggcggcctg gaaggcagaa gcgacatggc cctgaagctc gtgggcgggg
     3541 gccacctgat ctgcaacttg aagaccacat acagatccaa gaaacccgct aagaacctca
     3601 agatgcccgg cgtctactat gtggacagaa gactggaaag aatcaaggag gccgacaaag
     3661 agacctacgt cgagcagcac gaggtggctg tggccagata ctgcgacctc cctagcaaac
     3721 tggggcacaa acttaattag gcaccgcttg aagtctttaa ttaaataccg gtcgatatca
     3781 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     3841 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     3901 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     3961 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     4021 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     4081 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     4141 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     4201 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     4261 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     4321 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     4381 atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag
     4441 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
     4501 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     4561 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata
     4621 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
     4681 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     4741 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga
     4801 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc
     4861 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag
     4921 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     4981 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     5041 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc
     5101 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     5161 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     5221 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     5281 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     5341 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     5401 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     5461 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     5521 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     5581 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     5641 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     5701 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     5761 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     5821 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     5881 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     5941 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     6001 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6061 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     6121 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     6181 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
     6241 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt
     6301 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
     6361 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
     6421 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
     6481 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
     6541 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
     6601 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga
     6661 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     6721 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     6781 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6841 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6901 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     6961 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7021 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7081 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7141 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7201 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7261 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7321 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7381 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7441 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7501 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7561 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7621 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7681 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7741 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7801 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7861 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7921 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7981 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8041 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8101 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8161 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8221 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8281 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8341 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8401 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8461 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8521 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8581 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8641 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8701 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8761 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8821 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8881 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8941 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9001 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9061 aggggttccg cgcacatttc cccgaaaagt gccacctgac
//
