LOCUS       Exported                8668 bp ds-DNA     circular SYN 31-MAY-2017
DEFINITION  FuGW-G8p-huCCL21-1xBS(Pe).
ACCESSION   .
VERSION     .
KEYWORDS    SnapGene DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 2, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     GenBank                 8668 bp    DNA     circular SYN 29-MAY-2017
            ACCESSION   pLN491
            KEYWORDS:   .
FEATURES             Location/Qualifiers
     source          1..8668
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             245..260
                     /gene="cPPT"
                     /label=cPPT
     CDS             431..446
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             450..465
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             474..489
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             493..508
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             517..532
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             545..560
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             564..579
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             588..603
                     /gene="GAL4BS"
                     /label=GAL4BS
     CDS             610..650
                     /gene="lateADEp"
                     /product="major late promoter of adenovirus"
                     /label=lateADEp
                     /note="major late promoter of adenovirus"
     CDS             694..703
                     /gene="KOZAK"
                     /label=KOZAK
     CDS             700..768
                     /codon_start=1
                     /gene="signal peptide"
                     /product="hu CCL21 signal peptide"
                     /label=signal peptide
                     /note="hu CCL21 signal peptide"
                     /translation="MAQSLALSLLILVLAFGIPRTQG"
     CDS             769..1101
                     /codon_start=1
                     /gene="hu CCL21"
                     /product="hu CCL21 protein"
                     /label=hu CCL21
                     /note="hu CCL21 protein"
                     /translation="SDGGAQDCCLKYSQRKIPAKVVRSYRKQEPSLGCSIPAILFLPRK
                     RSQAELCADPKELWVQQLMQHLDKTPSPQKPAQGCRKDRGASKTGKKGKGSKGCKRTER
                     SQTPKGP"
     CDS             1110..1137
                     /gene="BS(Pe)"
                     /label=BS(Pe)
     CDS             1163..1750
                     /gene="WPRE"
                     /product="The WPRE seems to increase the expression of the 
                     marker gene when introduced in its 3' untranslated region. 
                     This element is believed to exert some posttranslational 
                     regulation. "
                     /label=WPRE
                     /note="The WPRE seems to increase the expression of the 
                     marker gene when introduced in its 3' untranslated region. 
                     This element is believed to exert some posttranslational 
                     regulation."
     CDS             1937..1958
                     /gene="U3PPT"
                     /product="reverse transcriptase-associated RNase H cleavage
                     of the RNA at the polypurine tract (PPT), a short (ca. 11 
                     to 19 nucleotides [nt]) string of purines located at the 
                     upstream border of the 3' long terminal repeat "
                     /label=U3PPT
                     /note="reverse transcriptase-associated RNase H cleavage of
                     the RNA at the polypurine tract (PPT), a short (ca. 11 to 
                     19 nucleotides [nt]) string of purines located at the 
                     upstream border of the 3' long terminal repeat"
     CDS             1937..1952
                     /gene="cPPT"
                     /label=cPPT
     CDS             2274..2454
                     /gene="del3'LTR"
                     /product="truncHIV-1_3_LTR"
                     /label=del3'LTR
                     /note="truncHIV-1_3_LTR"
     CDS             2483..2710
                     /gene="bGH-Ter"
                     /product="bGH_PA_terminator"
                     /label=bGH-Ter
                     /note="bGH_PA_terminator"
     CDS             complement(3199..3414)
                     /gene="SV40_enhancer"
                     /label=SV40_enhancer
     CDS             3211..3479
                     /gene="SV40p"
                     /label=SV40p
     CDS             3378..3455
                     /gene="SV40-Ori"
                     /label=SV40-Ori
     CDS             3573..3640
                     /gene="EM7p"
                     /label=EM7p
     CDS             3641..4012
                     /codon_start=1
                     /gene="Bleo"
                     /label=Bleo
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     CDS             4148..4267
                     /gene="SV40-pA"
                     /label=SV40-pA
     CDS             complement(5476..6336)
                     /codon_start=1
                     /gene="Amp"
                     /label=Amp
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(6378..6406)
                     /gene="Ampp"
                     /label=Ampp
     CDS             6708..7284
                     /gene="CMVp"
                     /product="CMV_immearly_promoter"
                     /label=CMVp
                     /note="CMV_immearly_promoter"
     CDS             6787..7074
                     /gene="CAG_enhancer"
                     /label=CAG_enhancer
     CDS             7304..397
                     /gene="5' Viral freatures'"
                     /label=5' Viral freatures'
     CDS             7304..7484
                     /gene="5'LTR"
                     /product="HIV-1_5_LTR"
                     /label=5'LTR
                     /note="HIV-1_5_LTR"
     CDS             7595..7639
                     /gene="Psi"
                     /label=Psi
     CDS             8149..8382
                     /gene="RRE"
                     /label=RRE
ORIGIN
        1 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
       61 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
      121 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
      181 gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa
      241 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
      301 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      361 ggtttattac agggacagca gagatccagt ttggttaatc ctgcaggcga taggtaccga
      421 gtttctagac ggagtactgt cctccgagcg gagtactgtc ctccgactcg agcggagtac
      481 tgtcctccga tcggagtact gtcctccgcg aattccggag tactgtcctc cgaagacgct
      541 agacggagta ctgtcctccg agcggagtac tgtcctccga ctcgagcgga gtactgtcct
      601 ccggctagcg gggggctata aaagggggtg ggggcgttcg tcctcactct agatctgcga
      661 tctaagtaag cttggcattc cggtactggg cgcgccacca tggctcaatc cttggcgctg
      721 tccttgctga tcttggtgct ggcatttggc attccccgca cacaaggaag cgatggtggc
      781 gcacaggact gttgcctgaa gtattcacag cggaaaatcc cagctaaagt ggttcggagc
      841 tatcgcaagc aggaaccttc cttggggtgt tcaatcccag ctatcctctt tcttccaaga
      901 aagcgctctc aggccgagct gtgtgctgac ccaaaagagc tgtgggtcca gcagttgatg
      961 cagcacttgg acaagacgcc gtctccccag aaacccgccc agggctgcag aaaagatagg
     1021 ggcgcctcaa aaacaggcaa aaaagggaaa ggtagcaaag gttgcaaacg caccgaacga
     1081 agccagacgc caaaagggcc ataaccggtg caccgcttga agtctttaat taaataccgg
     1141 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     1201 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     1261 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     1321 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     1381 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     1441 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     1501 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     1561 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     1621 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     1681 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     1741 cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta
     1801 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     1861 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     1921 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     1981 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     2041 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     2101 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     2161 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     2221 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     2281 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     2341 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     2401 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     2461 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     2521 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     2581 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     2641 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     2701 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     2761 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     2821 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     2881 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     2941 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     3001 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     3061 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     3121 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     3181 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     3241 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     3301 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     3361 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     3421 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     3481 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     3541 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     3601 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     3661 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     3721 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     3781 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     3841 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     3901 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     3961 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     4021 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     4081 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     4141 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     4201 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     4261 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     4321 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     4381 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     4441 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     4501 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     4561 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     4621 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     4681 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     4741 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     4801 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     4861 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     4921 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4981 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     5041 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     5101 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     5161 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     5221 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     5281 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     5341 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     5401 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     5461 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     5521 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     5581 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     5641 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     5701 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     5761 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     5821 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     5881 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     5941 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     6001 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     6061 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     6121 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     6181 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     6241 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     6301 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     6361 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     6421 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     6481 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     6541 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     6601 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     6661 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
     6721 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     6781 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     6841 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     6901 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     6961 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     7021 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     7081 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     7141 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     7201 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     7261 gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga
     7321 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7381 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7441 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     7501 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     7561 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     7621 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     7681 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     7741 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     7801 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     7861 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     7921 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     7981 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     8041 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     8101 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     8161 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     8221 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     8281 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     8341 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     8401 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     8461 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     8521 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     8581 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     8641 gtggtatata aaattattca taatgata
//
