LOCUS       Exported                4693 bp ds-DNA     circular SYN 04-DEC-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    VAMP2-SNAP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 20, 2018 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    27..549
                     /label=Pcmv
     intron          672..768
                     /label=SV40 SD/SA
     misc_feature    816..832
                     /label=MCS
     CDS             838..1185
                     /codon_start=1
                     /label=VAMP2
                     /translation="MSATAATVPPAAPAGEGGPPAPPPNLTSNRRLQQTQAQVDEVVDI
                     MRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKRKYWWKNLKMMIILGVICA
                     IILIIIIVYFST"
     CDS             1186..1203
                     /codon_start=1
                     /label=Linker
                     /translation="GGSGGS"
     CDS             1204..1776
                     /codon_start=1
                     /label=Snap tag
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYQQLAALAGNPAATAAVKTALSGNPVPILIPCHRVVSSSGAVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLGPAGIGAPGS"
     misc_feature    1794..1820
                     /label=FLAG
     polyA_signal    1955..1974
                     /label=SV40 poly A
     rep_origin      2447..3090
                     /label=pUC ORI
     CDS             complement(3238..4098)
                     /codon_start=1
                     /label=ampR
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaggcc cgaattcatg
      841 tcggctaccg ctgccaccgt cccgcctgcc gccccggccg gcgagggtgg cccccctgca
      901 cctcctccaa atcttaccag taacaggaga ctgcagcaga cccaggccca ggtggatgag
      961 gtggtggaca tcatgagggt gaatgtggac aaggtcctgg agcgggacca gaagctatcg
     1021 gaactggatg atcgcgcaga tgccctccag gcaggggcct cccagtttga aacaagtgca
     1081 gccaagctca agcgcaaata ctggtggaaa aacctcaaga tgatgatcat cttgggagtg
     1141 atttgcgcca tcatcctcat catcatcatc gtttacttca gcactggcgg atccggcgga
     1201 tccatggaca aagattgcga aatgaaacgt accaccctgg atagcccgct gggcaaactg
     1261 gaactgagcg gctgcgaaca gggcctgcat gaaattaaac tgctgggtaa aggcaccagc
     1321 gcggccgatg cggttgaagt tccggccccg gccgccgtgc tgggtggtcc ggaaccgctg
     1381 atgcaggcga ccgcgtggct gaacgcgtat tttcatcagc cggaagcgat tgaagaattt
     1441 ccggttccgg cgctgcatca tccggtgttt cagcaggaga gctttacccg tcaggtgctg
     1501 tggaaactgc tgaaagtggt taaatttggc gaagtgatta gctatcagca gctggcggcc
     1561 ctggcgggta atccggcggc caccgccgcc gttaaaaccg cgctgagcgg taacccggtg
     1621 ccgattctga ttccgtgcca tcgtgtggtt agctctagcg gtgcggttgg cggttatgaa
     1681 ggtggtctgg cggtgaaaga gtggctgctg gcccatgaag gtcatcgtct gggtaaaccg
     1741 ggtctgggac ctgcaggtat aggcgcgcca ggatcctaac tcgaggtacc ggcgactaca
     1801 aggacgacga tgacaagtaa gcggccgcgg ggatccagac atgataagat acattgatga
     1861 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     1921 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     1981 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttcgg atcctctaga
     2041 gtcgatctgc aggcatgcta gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     2101 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     2161 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     2221 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     2281 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     2341 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2401 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2461 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2521 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2581 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2641 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2701 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2761 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2821 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2881 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     2941 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3001 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3061 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3121 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     3181 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     3241 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     3301 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     3361 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3421 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3481 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3541 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3601 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3661 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3721 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3781 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3841 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3901 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3961 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     4021 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     4081 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     4141 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     4201 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     4261 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     4321 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     4381 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     4441 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     4501 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
     4561 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     4621 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     4681 aacgacggcc agt
//
