LOCUS Exported 5063 bp ds-DNA circular SYN 04-DEC-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS VAMP2-pHuji SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5063) AUTHORS Justin Taraska TITLE Direct Submission JOURNAL Exported Tuesday, Mar 20, 2018 from SnapGene 3.3.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5063 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 212..415 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" CDS 481..828 /codon_start=1 /label=VAMP2 /translation="MSATAATVPPAAPAGEGGPPAPPPNLTSNRRLQQTQAQVDEVVDI MRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKRKYWWKNLKMMIILGVICA IILIIIIVYFST" CDS 865..1572 /codon_start=1 /product="photostable monomeric derivative of DsRed (Shaner et al., 2008) mapple K163Y" /label=pHuji /note="mammalian codon-optimized" /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALY SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA EGRHSTGGMDELYK" polyA_signal 1698..1819 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(1826..2281) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2308..2412 /gene="bla" /label=AmpR promoter promoter 2414..2771 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 2622..2757 /label=SV40 ori /note="SV40 origin of replication" CDS 2806..3600 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 3832..3879 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4208..4796 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" enhancer 4971..211 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt 61 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa 121 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta 181 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg 241 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg 301 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc 361 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt 421 agtgaaccgt cagatccgct agcgctaccg gactcagatc tcgagctcaa gcttgccacc 481 atgtcggcta ccgctgccac cgtcccgcct gccgccccgg ccggcgaggg tggcccccct 541 gcacctcctc caaatcttac cagtaacagg agactgcagc agacccaggc ccaggtggat 601 gaggtggtgg acatcatgag ggtgaatgtg gacaaggtcc tggagcggga ccagaagcta 661 tcggaactgg atgatcgcgc agatgccctc caggcagggg cctcccagtt tgaaacaagt 721 gcagccaagc tcaagcgcaa atactggtgg aaaaacctca agatgatgat catcttggga 781 gtgatttgcg ccatcatcct catcatcatc atcgtttact tcagcactgg ggtaccgcgg 841 gcccgggatc caccggtcgc caccatggtg agcaagggcg aggagaataa catggccatc 901 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc 961 gagatcgagg gcgagggcga gggccgcccc tacgaggcct ttcagaccgc taagctgaag 1021 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac 1081 ggctccaagg tctacattaa gcacccagcc gacatccccg actacttcaa gctgtccttc 1141 cccgagggct tcaggtggga gcgcgtgatg aacttcgagg acggcggcat tattcacgtt 1201 aaccaggact cctccctgca ggacggcgtg ttcatctaca aggtgaagct gcgcggcacc 1261 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccgag 1321 gagcggatgt accccgagga cggcgccctg tacagcgaga tcaagaagag gctgaagctg 1381 aaggacggcg gccactacgc cgccgaggtc aagaccacct acaaggccaa gaagcccgtg 1441 cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcgtgtccca caacgaggac 1501 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac 1561 gagctgtaca agtaaagcgg ccgcgactct agatcataat cagccatacc acatttgtag 1621 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga 1681 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata 1741 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 1801 aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg 1861 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct 1921 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt 1981 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat 2041 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca 2101 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac 2161 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta 2221 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg 2281 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 2341 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 2401 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg 2461 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 2521 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 2581 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 2641 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 2701 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 2761 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat 2821 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca 2881 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg 2941 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg 3001 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact 3061 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct 3121 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg 3181 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt 3241 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc 3301 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc 3361 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga 3421 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc 3481 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt 3541 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga 3601 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt 3661 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg 3721 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga 3781 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa 3841 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc 3901 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt 3961 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg 4021 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa 4081 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 4141 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 4201 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 4261 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 4321 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 4381 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 4441 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 4501 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 4561 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 4621 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 4681 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 4741 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 4801 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 4861 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat tagttattaa 4921 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa 4981 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata 5041 atgacgtatg ttcccatagt aac //