LOCUS       Exported                5063 bp ds-DNA     circular SYN 04-DEC-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    VAMP2-pHuji
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Justin Taraska
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 20, 2018 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        212..415
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             481..828
                     /codon_start=1
                     /label=VAMP2
                     /translation="MSATAATVPPAAPAGEGGPPAPPPNLTSNRRLQQTQAQVDEVVDI
                     MRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKRKYWWKNLKMMIILGVICA
                     IILIIIIVYFST"
     CDS             865..1572
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)
                     
                     mapple K163Y"
                     /label=pHuji
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALY
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    1698..1819
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1826..2281)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2308..2412
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2414..2771
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2622..2757
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2806..3600
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3832..3879
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4208..4796
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     enhancer        4971..211
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
       61 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      121 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      181 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      241 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      301 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      361 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt
      421 agtgaaccgt cagatccgct agcgctaccg gactcagatc tcgagctcaa gcttgccacc
      481 atgtcggcta ccgctgccac cgtcccgcct gccgccccgg ccggcgaggg tggcccccct
      541 gcacctcctc caaatcttac cagtaacagg agactgcagc agacccaggc ccaggtggat
      601 gaggtggtgg acatcatgag ggtgaatgtg gacaaggtcc tggagcggga ccagaagcta
      661 tcggaactgg atgatcgcgc agatgccctc caggcagggg cctcccagtt tgaaacaagt
      721 gcagccaagc tcaagcgcaa atactggtgg aaaaacctca agatgatgat catcttggga
      781 gtgatttgcg ccatcatcct catcatcatc atcgtttact tcagcactgg ggtaccgcgg
      841 gcccgggatc caccggtcgc caccatggtg agcaagggcg aggagaataa catggccatc
      901 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
      961 gagatcgagg gcgagggcga gggccgcccc tacgaggcct ttcagaccgc taagctgaag
     1021 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     1081 ggctccaagg tctacattaa gcacccagcc gacatccccg actacttcaa gctgtccttc
     1141 cccgagggct tcaggtggga gcgcgtgatg aacttcgagg acggcggcat tattcacgtt
     1201 aaccaggact cctccctgca ggacggcgtg ttcatctaca aggtgaagct gcgcggcacc
     1261 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccgag
     1321 gagcggatgt accccgagga cggcgccctg tacagcgaga tcaagaagag gctgaagctg
     1381 aaggacggcg gccactacgc cgccgaggtc aagaccacct acaaggccaa gaagcccgtg
     1441 cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcgtgtccca caacgaggac
     1501 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     1561 gagctgtaca agtaaagcgg ccgcgactct agatcataat cagccatacc acatttgtag
     1621 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     1681 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     1741 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     1801 aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg
     1861 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     1921 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     1981 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     2041 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     2101 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     2161 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     2221 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     2281 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     2341 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     2401 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     2461 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     2521 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     2581 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     2641 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     2701 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     2761 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     2821 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     2881 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     2941 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
     3001 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     3061 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     3121 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     3181 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     3241 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     3301 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
     3361 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     3421 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     3481 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     3541 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     3601 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     3661 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     3721 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga
     3781 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
     3841 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     3901 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     3961 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     4021 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa
     4081 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     4141 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4201 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4261 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4321 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     4381 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4441 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4501 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4561 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4621 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4681 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4741 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4801 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4861 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat tagttattaa
     4921 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     4981 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     5041 atgacgtatg ttcccatagt aac
//
