LOCUS       pJOG130-pEGG_AATG-GCTT[CDS1]_corrected      4807 bp    DNA     circular UNA 06-Aug-2013
DEFINITION  pENTR/D (Invitrogen) modified to contain CACC-BsaI-ccdB-Cm-AAGG between attL sites. Product after cloning with BsaI is identical TOPO reaction product
FEATURES             Location/Qualifiers
     primer          517..533
                     /label=M13f
     misc_recomb     569..668
                     /note="Match:
                     CAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGC
                     AATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCT"
                     /note="Length of match: 100"
                     /note="Accuracy (%): 100"
                     /note=Description:
                     /note="Found at strand: Positive"
                     /label=attL1/2
     CDS             790..1449
                     /gene="cat"
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="BAF47416.1"
                     /db_xref="GI:126148711"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            790..1449
                     /gene="cat"
     CDS             1791..2096
                     /gene="ccdB"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="BAF47417.1"
                     /db_xref="GI:126148712"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            1791..2096
                     /gene="ccdB"
     misc_recomb     complement(2157..2256)
                     /note="Match:
                     CAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATAAGC
                     AATGCTTTCTTATAATGCCAACTTTGTACAAGAAAGCTGGGT"
                     /note="Length of match: 100"
                     /note="Accuracy (%): 95"
                     /note=Description:
                     /note="Found at strand: Negative"
                     /label=attL1/2
     primer          complement(2297..2313)
                     /label=M13r
     misc_feature    2343..3129
                     /feature_type=Insertion
                     /note="accidental insertion of Transposon [?], which
                     occured during vector preparation"
     Region          4055..4747
                     /note="Match:
                     GACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTACGCGTCG
                     TTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTT
                     TTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTG
                     TTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCG
                     CAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACT
                     CTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAG
                     TGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCG
                     CAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCT
                     ACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGG
                     GAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGG
                     GAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCT
                     GACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAA"
                     /note="Length of match: 693"
                     /note="Accuracy (%): 92"
                     /note="Description: origin of replication"
                     /note="Found at strand: Positive"
                     /label="ColE1 ori"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTCC GCGGCCGCaa tgTgagaccA TTAGGCACCC CAGGCTTTAC ACTTTATGCT
      721 TCCGGCTCGT ATAATGTGTG GATTTTGAGT TAGGATCCGG CGAGATTTTC AGGAGCTAAG
      781 GAAGCTAAAA TGGAGAAAAA AATCACTGGA TATACCACCG TTGATATATC CCAATGGCAT
      841 CGTAAAGAAC ATTTTGAGGC ATTTCAGTCA GTTGCTCAAT GTACCTATAA CCAGACCGTT
      901 CAGCTGGATA TTACGGCCTT TTTAAAGACC GTAAAGAAAA ATAAGCACAA GTTTTATCCG
      961 GCCTTTATTC ACATTCTTGC CCGCCTGATG AATGCTCATC CGGAATTCCG TATGGCAATG
     1021 AAAGACGGTG AGCTGGTGAT ATGGGATAGT GTTCACCCTT GTTACACCGT TTTCCATGAG
     1081 CAAACTGAAA CGTTTTCATC GCTCTGGAGT GAATACCACG ACGATTTCCG GCAGTTTCTA
     1141 CACATATATT CGCAAGATGT GGCGTGTTAC GGTGAAAACC TGGCCTATTT CCCTAAAGGG
     1201 TTTATTGAGA ATATGTTTTT CGTCTCAGCC AATCCCTGGG TGAGTTTCAC CAGTTTTGAT
     1261 TTAAACGTGG CCAATATGGA CAACTTCTTC GCCCCCGTTT TCACCATGGG CAAATATTAT
     1321 ACGCAAGGCG ACAAGGTGCT GATGCCGCTG GCGATTCAGG TTCATCATGC CGTCTGTGAT
     1381 GGCTTCCATG TCGGCAGAAT GCTTAATGAA TTACAACAGT ACTGCGATGA GTGGCAGGGC
     1441 GGGGCGTAAA GATCTGGATC CGGCTTACTA AAAGCCAGAT AACAGTATGC GTATTTGCGC
     1501 GCTGATTTTT GCGGTATAAG AATATATACT GATATGTATA CCCGAAGTAT GTCAAAAAGA
     1561 GGTGTGCTAT GAAGCAGCGT ATTACAGTGA CAGTTGACAG CGACAGCTAT CAGTTGCTCA
     1621 AGGCATATAT GATGTCAATA TCTCCGGTCT GGTAAGCACA ACCATGCAGA ATGAAGCCCG
     1681 TCGTCTGCGT GCCGAACGCT GGAAAGCGGA AAATCAGGAA GGGATGGCTG AGGTCGCCCG
     1741 GTTTATTGAA ATGAACGGCT CTTTTGCTGA CGAGAACAGG GACTGGTGAA ATGCAGTTTA
     1801 AGGTTTACAC CTATAAAAGA GAGAGCCGTT ATCGTCTGTT TGTGGATGTA CAGAGTGATA
     1861 TTATTGACAC GCCCGGGCGA CGGATGGTGA TCCCCCTGGC CAGTGCACGT CTGCTGTCAG
     1921 ATAAAGTCTC CCGTGAACTT TACCCGGTGG TGCATATCGG GGATGAAAGC TGGCGCATGA
     1981 TGACCACCGA TATGGCCAGT GTGCCGGTtT CCGTTATCGG GGAAGAAGTG GCTGATCTCA
     2041 GCCACCGCGA AAATGACATC AAAAACGCCA TTAACCTGAT GTTCTGGGGA ATATAAATGT
     2101 CAGGCTCCCT TATACACAGC CAGTCTGCAG GTCGACGGTC TCAgcttggC GCGCCGACCC
     2161 AGCTTTCTTG TACAAAGTTG GCATTATAAG AAAGCATTGC TTATCAATTT GTTGCAACGA
     2221 ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTTGCCAT CCAGCTGATA TCCCCTATAG
     2281 TGAGTCGTAT TACATGGTCA TAGCTGTTTC CTGGCAGCTC TGGCCCGTGT CTCAAAATCT
     2341 CTGATGTTAC ATTGGGTAAT GACTCCAACT TATTGATAGT GTTTTATGTT CAGATAATGC
     2401 CCGATGACTT TGTCATGCAG CTCCACCGAT TTTGAGAACG ACAGCGACTT CCGTCCCAGC
     2461 CGTGCCAGGT GCTGCCTCAG ATTCAGGTTA TGCCGCTCAA TTCGCTGCGT ATATCGCTTG
     2521 CTGATTACGT GCAGCTTTCC CTTCAGGCGG GATTCATACA GCGGCCAGCC ATCCGTCATC
     2581 CATATCACCA CGTCAAAGGG TGACAGCAGG CTCATAAGAC GCCCCAGCGT CGCCATAGTG
     2641 CGTTCACCGA ATACGTGCGC AACAACCGTC TTCCGGAGAC TGTCATACGC GTAAAACAGC
     2701 CAGCGCTGGC GCGATTTAGC CCCGACATAG CCCCACTGTT CGTCCATTTC CGCGCAGACG
     2761 ATGACGTCAC TGCCCGGCTG TATGCGCGAG GTTACCGACT GCGGCCTGAG TTTTTTAAGT
     2821 GACGTAAAAT CGTGTTGAGG CCAACGCCCA TAATGCGGGC TGTTGCCCGG CATCCAACGC
     2881 CATTCATGGC CATATCAATG ATTTTCTGGT GCGTACCGGG TTGAGAAGCG GTGTAAGTGA
     2941 ACTGCAGTTG CCATGTTTTA CGGCAGTGAG AGCAGAGATA GCGCTGATGT CCGGCGGTGC
     3001 TTTTGCCGTT ACGCACCACC CCGTCAGTAG CTGACAGGAG GGACAGCTGA TAGAAACAGA
     3061 AGCCACTGGA GCACCTCAAA AACACCATCA TACACTAAAT CAGTAAGTTG GCAGCATCAC
     3121 CTTACATTGC ACAAGATAAA AATATATCAT CATGAACAAT AAAACTGTCT GCTTACATAA
     3181 ACAGTAATAC AAGGGGTGTT ATGAGCCATA TTCAACGGGA AACGTCGAGG CCGCGATTAA
     3241 ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT GTCGGGCAAT
     3301 CAGGTGCGAC AATCTATCGC TTGTATGGGA AGCCCGATGC GCCAGAGTTG TTTCTGAAAC
     3361 ATGGCAAAGG TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA
     3421 CGGAATTTAT GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT
     3481 TACTCACCAC TGCGATCCCC GGAAAAACAG CATTCCAGGT ATTAGAAGAA TATCCTGATT
     3541 CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT TCGATTCCTG
     3601 TTTGTAATTG TCCTTTTAAC AGCGATCGCG TATTTCGTCT CGCTCAGGCG CAATCACGAA
     3661 TGAATAACGG TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG
     3721 AACAAGTCTG GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC
     3781 ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA GGTTGTATTG
     3841 ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA TGGAACTGCC
     3901 TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT ATTGATAATC
     3961 CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA TCAGAATTGG
     4021 TTAATTGGTT GTAACACTGG CAGAGCATTA CGCTGACTTG ACGGGACGGC GCAAGCTCAT
     4081 GACCAAAATC CCTTAACGTG AGTTACGCGT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA
     4141 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
     4201 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
     4261 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT
     4321 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC
     4381 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
     4441 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
     4501 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG
     4561 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG
     4621 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT
     4681 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
     4741 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC
     4801 ACATGTT
//
