LOCUS       pJOG131-pEGG_AATG-TTCG[CDS1ns]_corrected      4808 bp    DNA     circular UNA 06-Aug-2013
DEFINITION  pENTR/D (Invitrogen) modified to contain CACC-BsaI-ccdB-Cm-AAGG between attL sites. Product after cloning with BsaI is identical TOPO reaction product
FEATURES             Location/Qualifiers
     primer          517..533
                     /label=M13f
     misc_recomb     569..668
                     /note="Match:
                     CAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGC
                     AATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCT"
                     /note="Length of match: 100"
                     /note="Accuracy (%): 100"
                     /note=Description:
                     /note="Found at strand: Positive"
                     /label=attL1/2
     CDS             790..1449
                     /gene="cat"
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="BAF47416.1"
                     /db_xref="GI:126148711"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            790..1449
                     /gene="cat"
     CDS             1791..2096
                     /gene="ccdB"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="BAF47417.1"
                     /db_xref="GI:126148712"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            1791..2096
                     /gene="ccdB"
     misc_recomb     complement(2158..2257)
                     /note="Match:
                     CAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATAAGC
                     AATGCTTTCTTATAATGCCAACTTTGTACAAGAAAGCTGGGT"
                     /note="Length of match: 100"
                     /note="Accuracy (%): 95"
                     /note=Description:
                     /note="Found at strand: Negative"
                     /label=attL1/2
     primer          complement(2298..2314)
                     /label=M13r
     misc_feature    2344..3130
                     /feature_type=Insertion
                     /note="accidental insertion of Transposon [?], which
                     occured during vector preparation"
     CDS             3202..4011
                     /label="nptII (Kan-R)"
     rep_origin      4056..4748
                     /note="Match:
                     GACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTACGCGTCG
                     TTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTT
                     TTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTG
                     TTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCG
                     CAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACT
                     CTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAG
                     TGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCG
                     CAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCT
                     ACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGG
                     GAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGG
                     GAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCT
                     GACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAA"
                     /note="Length of match: 693"
                     /note="Accuracy (%): 92"
                     /note="Description: origin of replication"
                     /note="Found at strand: Positive"
                     /label="ColE1 ori"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTCC GCGGCCGCaa tgTgagaccA TTAGGCACCC CAGGCTTTAC ACTTTATGCT
      721 TCCGGCTCGT ATAATGTGTG GATTTTGAGT TAGGATCCGG CGAGATTTTC AGGAGCTAAG
      781 GAAGCTAAAA TGGAGAAAAA AATCACTGGA TATACCACCG TTGATATATC CCAATGGCAT
      841 CGTAAAGAAC ATTTTGAGGC ATTTCAGTCA GTTGCTCAAT GTACCTATAA CCAGACCGTT
      901 CAGCTGGATA TTACGGCCTT TTTAAAGACC GTAAAGAAAA ATAAGCACAA GTTTTATCCG
      961 GCCTTTATTC ACATTCTTGC CCGCCTGATG AATGCTCATC CGGAATTCCG TATGGCAATG
     1021 AAAGACGGTG AGCTGGTGAT ATGGGATAGT GTTCACCCTT GTTACACCGT TTTCCATGAG
     1081 CAAACTGAAA CGTTTTCATC GCTCTGGAGT GAATACCACG ACGATTTCCG GCAGTTTCTA
     1141 CACATATATT CGCAAGATGT GGCGTGTTAC GGTGAAAACC TGGCCTATTT CCCTAAAGGG
     1201 TTTATTGAGA ATATGTTTTT CGTCTCAGCC AATCCCTGGG TGAGTTTCAC CAGTTTTGAT
     1261 TTAAACGTGG CCAATATGGA CAACTTCTTC GCCCCCGTTT TCACCATGGG CAAATATTAT
     1321 ACGCAAGGCG ACAAGGTGCT GATGCCGCTG GCGATTCAGG TTCATCATGC CGTCTGTGAT
     1381 GGCTTCCATG TCGGCAGAAT GCTTAATGAA TTACAACAGT ACTGCGATGA GTGGCAGGGC
     1441 GGGGCGTAAA GATCTGGATC CGGCTTACTA AAAGCCAGAT AACAGTATGC GTATTTGCGC
     1501 GCTGATTTTT GCGGTATAAG AATATATACT GATATGTATA CCCGAAGTAT GTCAAAAAGA
     1561 GGTGTGCTAT GAAGCAGCGT ATTACAGTGA CAGTTGACAG CGACAGCTAT CAGTTGCTCA
     1621 AGGCATATAT GATGTCAATA TCTCCGGTCT GGTAAGCACA ACCATGCAGA ATGAAGCCCG
     1681 TCGTCTGCGT GCCGAACGCT GGAAAGCGGA AAATCAGGAA GGGATGGCTG AGGTCGCCCG
     1741 GTTTATTGAA ATGAACGGCT CTTTTGCTGA CGAGAACAGG GACTGGTGAA ATGCAGTTTA
     1801 AGGTTTACAC CTATAAAAGA GAGAGCCGTT ATCGTCTGTT TGTGGATGTA CAGAGTGATA
     1861 TTATTGACAC GCCCGGGCGA CGGATGGTGA TCCCCCTGGC CAGTGCACGT CTGCTGTCAG
     1921 ATAAAGTCTC CCGTGAACTT TACCCGGTGG TGCATATCGG GGATGAAAGC TGGCGCATGA
     1981 TGACCACCGA TATGGCCAGT GTGCCGGTtT CCGTTATCGG GGAAGAAGTG GCTGATCTCA
     2041 GCCACCGCGA AAATGACATC AAAAACGCCA TTAACCTGAT GTTCTGGGGA ATATAAATGT
     2101 CAGGCTCCCT TATACACAGC CAGTCTGCAG GTCGACGGTC TCAttcgggg CGCGCCGACC
     2161 CAGCTTTCTT GTACAAAGTT GGCATTATAA GAAAGCATTG CTTATCAATT TGTTGCAACG
     2221 AACAGGTCAC TATCAGTCAA AATAAAATCA TTATTTGCCA TCCAGCTGAT ATCCCCTATA
     2281 GTGAGTCGTA TTACATGGTC ATAGCTGTTT CCTGGCAGCT CTGGCCCGTG TCTCAAAATC
     2341 TCTGATGTTA CATTGGGTAA TGACTCCAAC TTATTGATAG TGTTTTATGT TCAGATAATG
     2401 CCCGATGACT TTGTCATGCA GCTCCACCGA TTTTGAGAAC GACAGCGACT TCCGTCCCAG
     2461 CCGTGCCAGG TGCTGCCTCA GATTCAGGTT ATGCCGCTCA ATTCGCTGCG TATATCGCTT
     2521 GCTGATTACG TGCAGCTTTC CCTTCAGGCG GGATTCATAC AGCGGCCAGC CATCCGTCAT
     2581 CCATATCACC ACGTCAAAGG GTGACAGCAG GCTCATAAGA CGCCCCAGCG TCGCCATAGT
     2641 GCGTTCACCG AATACGTGCG CAACAACCGT CTTCCGGAGA CTGTCATACG CGTAAAACAG
     2701 CCAGCGCTGG CGCGATTTAG CCCCGACATA GCCCCACTGT TCGTCCATTT CCGCGCAGAC
     2761 GATGACGTCA CTGCCCGGCT GTATGCGCGA GGTTACCGAC TGCGGCCTGA GTTTTTTAAG
     2821 TGACGTAAAA TCGTGTTGAG GCCAACGCCC ATAATGCGGG CTGTTGCCCG GCATCCAACG
     2881 CCATTCATGG CCATATCAAT GATTTTCTGG TGCGTACCGG GTTGAGAAGC GGTGTAAGTG
     2941 AACTGCAGTT GCCATGTTTT ACGGCAGTGA GAGCAGAGAT AGCGCTGATG TCCGGCGGTG
     3001 CTTTTGCCGT TACGCACCAC CCCGTCAGTA GCTGACAGGA GGGACAGCTG ATAGAAACAG
     3061 AAGCCACTGG AGCACCTCAA AAACACCATC ATACACTAAA TCAGTAAGTT GGCAGCATCA
     3121 CCTTACATTG CACAAGATAA AAATATATCA TCATGAACAA TAAAACTGTC TGCTTACATA
     3181 AACAGTAATA CAAGGGGTGT TATGAGCCAT ATTCAACGGG AAACGTCGAG GCCGCGATTA
     3241 AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG CTCGCGATAA TGTCGGGCAA
     3301 TCAGGTGCGA CAATCTATCG CTTGTATGGG AAGCCCGATG CGCCAGAGTT GTTTCTGAAA
     3361 CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA TGGTCAGACT AAACTGGCTG
     3421 ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC GTACTCCTGA TGATGCATGG
     3481 TTACTCACCA CTGCGATCCC CGGAAAAACA GCATTCCAGG TATTAGAAGA ATATCCTGAT
     3541 TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA TTCGATTCCT
     3601 GTTTGTAATT GTCCTTTTAA CAGCGATCGC GTATTTCGTC TCGCTCAGGC GCAATCACGA
     3661 ATGAATAACG GTTTGGTTGA TGCGAGTGAT TTTGATGACG AGCGTAATGG CTGGCCTGTT
     3721 GAACAAGTCT GGAAAGAAAT GCATAAACTT TTGCCATTCT CACCGGATTC AGTCGTCACT
     3781 CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG GGAAATTAAT AGGTTGTATT
     3841 GATGTTGGAC GAGTCGGAAT CGCAGACCGA TACCAGGATC TTGCCATCCT ATGGAACTGC
     3901 CTCGGTGAGT TTTCTCCTTC ATTACAGAAA CGGCTTTTTC AAAAATATGG TATTGATAAT
     3961 CCTGATATGA ATAAATTGCA GTTTCATTTG ATGCTCGATG AGTTTTTCTA ATCAGAATTG
     4021 GTTAATTGGT TGTAACACTG GCAGAGCATT ACGCTGACTT GACGGGACGG CGCAAGCTCA
     4081 TGACCAAAAT CCCTTAACGT GAGTTACGCG TCGTTCCACT GAGCGTCAGA CCCCGTAGAA
     4141 AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA
     4201 AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT
     4261 CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG
     4321 TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC
     4381 CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA
     4441 CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC
     4501 AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCA TTGAGAAAGC
     4561 GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA
     4621 GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG
     4681 TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA
     4741 TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT
     4801 CACATGTT
//
