LOCUS pCK013_AvrRPS4N_CDS1 6389 bp DNA circular SYN 10-JAN-2005 DEFINITION Cloning vector pBBR1MCS-5 REP, LacZ alpha peptide (lacZ alpha), and gentamicin-3-acetyltransferase genes, complete cds. ACCESSION U25061 VERSION U25061.1 GI:833825 KEYWORDS . SOURCE Cloning vector pBBR1MCS-5 (unknown) ORGANISM Cloning vector pBBR1MCS-5 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 4768) AUTHORS Kovach,M.E., Phillips,R.W., Elzer,P.H., Roop,R.M. II and Peterson,K.M. TITLE pBBR1MCS: a broad-host-range cloning vector JOURNAL BioTechniques 16 (5), 800-802 (1994) PUBMED 8068328 REFERENCE 2 (bases 1 to 4768) AUTHORS Kovach,M.E., Elzer,P.H., Hill,D.S., Robertson,G.T., Farris,M.A., Roop,R.M. II and Peterson,K.M. TITLE Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes JOURNAL Gene 166 (1), 175-176 (1995) PUBMED 8529885 REFERENCE 3 (bases 1 to 4768) AUTHORS Kovach,M.E. TITLE Direct Submission JOURNAL Submitted (13-APR-1995) Michael E. Kovach, Microbiology, Louisiana State University, 1501 Kings Highway, Shreveport, LA 71130, USA FEATURES Location/Qualifiers CDS complement(158..691) /function="resistance to gentamicin" /codon_start=1 /transl_table=11 /product="gentamicin-3-acetyltransferase" /protein_id="AAB06696.1" /db_xref="GI:833828" /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAAL DLFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQP RSEIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKL GIREEVMHFDIDPSTAT" primer 1077..1094 /label="pBR1180F" promoter 1123..1280 /label="plac" CDS 1286..>1687 /gene="avrRps4" /codon_start=1 /transl_table=11 /product="avirulence protein" /protein_id="AAB51082.1" /db_xref="GI:1008894" /translation="MTRISTSSVNSSFSYSAPAEEAQNRVSSAPTNSSPPGTTTAVAQ ASEGQKKPGAVLSMHAQRLRQLCESSSHQYRQNALLAKSFDAQRLDINTQAGPSNSPH LNALNKLQQRDFKPAAGGLEIPFTPKSLLGGGKRVYQIGSSSRDVQVCPRGAGAALRQ EIEDKQLMVNNLTDELQDAIDEANPAEIANTSQQLRHARADLADLQRRFAVLRNEDRR IN" primer 1541..1561 /label="JS770-sequencing primer" Site 1682..1687 /site_type="cleavage site" /label="predicted cleavage between Gyl133-Gly134" CDS 1812..2471 /invitrogen="1080000" /label="Cm(R)" CDS 2813..3118 /invitrogen="1060000" /label="ccdB" CDS 3155..3265 /label="3xmyc" primer complement(3334..3356) /label="pBRMenlaczR" CDS complement(3832..4494) /note="required for plasmid replication" /codon_start=1 /transl_table=11 /product="REP" /protein_id="AAB06694.1" /db_xref="GI:833826" /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP RR" ORIGIN 1 GCTGACGGAA CAGCGGGAAG TCCAGCGCCA GAAACAGGCC CAGCGCCAGC AGGAACGCGG 61 GCGCGCACAT TTCCCCGAAA AGTGCCACCT GGCGGCGTTG TGACAATTTA CCGAACAACT 121 CCGCGGCCGG GAAGCCGATC TCGGCTTGAA CGAATTGTTA GGTGGCGGTA CTTGGGTCGA 181 TATCAAAGTG CATCACTTCT TCCCGTATGC CCAACTTTGT ATAGAGAGCC ACTGCGGGAT 241 CGTCACCGTA ATCTGCTTGC ACGTAGATCA CATAAGCACC AAGCGCGTTG GCCTCATGCT 301 TGAGGAGATT GATGAGCGCG GTGGCAATGC CCTGCCTCCG GTGCTCGCCG GAGACTGCGA 361 GATCATAGAT ATAGATCTCA CTACGCGGCT GCTCAAACCT GGGCAGAACG TAAGCCGCGA 421 GAGCGCCAAC AACCGCTTCT TGGTCGAAGG CAGCAAGCGC GATGAATGTC TTACTACGGA 481 GCAAGTTCCC GAGGTAATCG GAGTCCGGCT GATGTTGGGA GTAGGTGGCT ACGTCTCCGA 541 ACTCACGACC GAAAAGATCA AGAGCAGCCC GCATGGATTT GACTTGGTCA GGGCCGAGCC 601 TACATGTGCG AATGATGCCC ATACTTGAGC CACCTAACTT TGTTTTAGGG CGACTGCCCT 661 GCTGCGTAAC ATCGTTGCTG CTGCGTAACA TCGTTGCTGC TCCATAACAT CAAACATCGA 721 CCCACGGCGT AACGCGCTTG CTGCTTGGAT GCCCGAGGCA TAGACTGTAC AAAAAAACAG 781 TCATAACAAG CCATGAAAAC CGCCACTGCG CCGTTACCAC CGCTGCGTTC GGTCAAGGTT 841 CTGGACCAGT TGCGTGAGCG CATACGCTAC TTGCATTACA GTTTACGAAC CGAACAGGCT 901 TATGTCAACT GGGTTCGTGC CTTCATCCGT TTCCACGGTG TGCGTCCATG GGCAAATATT 961 ATACGCAAGG CGACAAGGTG CTGATGCCGC TGGCGATTCA GGTTCATCAT GCCGTTTGTG 1021 ATGGCTTCCA TGTCGGCAGA ATGCTTAATG AATTACAACA GTTTTTATGC ATGCGCCCAA 1081 TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT 1141 TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT 1201 AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG 1261 GATAACAATT TCACACAGGA GAATTATGAC TCGAATTTCA ACCAGTTCAG TAAATTCCAG 1321 CTTCAGTTAC TCGGCCCCTG CAGAGGAAGC GCAAAACCGC GTTTCCTCAG CCCCCACCAA 1381 CTCCTCTCCA CCTGGCACCA CAACCGCTGT CGCCCAGGCG TCAGAGGGGC AAAAAAAGCC 1441 GGGGGCGGTA TTAAGCATGC ATGCACAGCG ACTGCGCCAA TTGTGTGAGA GTTCATCTCA 1501 CCAGTACCGG CAGAACGCGC TATTAGCTAA ATCTTTTGAT GCTCAACGCC TAGACATTAA 1561 CACTCAAGCG GGCCCTTCCA ATAGCCCTCA CCTGAACGCT CTCAACAAGC TCCAACAACG 1621 AGATTTCAAA CCTGCGGCTG GCGGGCTAGA AATCCCATTT ACACCCAAAT CCCTATTGGG 1681 TGGAGGTgca ATGagagacc TGAGACGTTG ATCGGCACGT AAGAGGTTCC AACTTTCACC 1741 ATAATGAAAT AAGATCACTA CCGGGCGTAT TTTTTGAGTT ATCGAGATTT TCAGGAGCTA 1801 AGGAAGCTAA AATGGAGAAA AAAATCACTG GATATACCAC CGTTGATATA TCCCAATGGC 1861 ATCGTAAAGA ACATTTTGAG GCATTTCAGT CAGTTGCTCA ATGTACCTAT AACCAGACCG 1921 TTCAGCTGGA TATTACGGCC TTTTTAAAGA CCGTAAAGAA AAATAAGCAC AAGTTTTATC 1981 CGGCCTTTAT TCACATTCTT GCCCGCCTGA TGAATGCTCA TCCGGAATTC CGTATGGCAA 2041 TGAAAGACGG TGAGCTGGTG ATATGGGATA GTGTTCACCC TTGTTACACC GTTTTCCATG 2101 AGCAAACTGA AACGTTTTCA TCGCTCTGGA GTGAATACCA CGACGATTTC CGGCAGTTTC 2161 TACACATATA TTCGCAAGAT GTGGCGTGTT ACGGTGAAAA CCTGGCCTAT TTCCCTAAAG 2221 GGTTTATTGA GAATATGTTT TTCGTCTCAG CCAATCCCTG GGTGAGTTTC ACCAGTTTTG 2281 ATTTAAACGT GGCCAATATG GACAACTTCT TCGCCCCCGT TTTCACCATG GGCAAATATT 2341 ATACGCAAGG CGACAAGGTG CTGATGCCGC TGGCGATTCA GGTTCATCAT GCCGTCTGTG 2401 ATGGCTTCCA TGTCGGCAGA ATGCTTAATG AATTACAACA GTACTGCGAT GAGTGGCAGG 2461 GCGGGGCGTA ATCGCGTGGA TCCGGCTTAC TAAAAGCCAG ATAACAGTAT GCGTATTTGC 2521 GCGCTGATTT TTGCGGTATA AGAATATATA CTGATATGTA TACCCGAAGT ATGTCAAAAA 2581 GAGGTaTGCT ATGAAGCAGC GTATTACAGT GACAGTTGAC AGCGACAGCT ATCAGTTGCT 2641 CAAGGCATAT ATGATGTCAA TATCTCCGGT CTGGTAAGCA CAACCATGCA GAATGAAGCC 2701 CGTCGTCTGC GTGCCGAACG CTGGAAAGCG GAAAATCAGG AAGGGATGGC TGAGGTCGCC 2761 CGGTTTATTG AAATGAACGG CTCTTTTGCT GACGAGAACA GGGgCTGGTG AAATGCAGTT 2821 TAAGGTTTAC ACCTATAAAA GAGAGAGCCG TTATCGTCTG TTTGTGGATG TACAGAGTGA 2881 TATTATTGAC ACGCCCGGGC GACGGATGGT GATCCCCCTG GCCAGTGCAC GTCTGCTGTC 2941 AGATAAAGTC TCCCGTGAAC TTTACCCGGT GGTGCATATC GGGGATGAAA GCTGGCGCAT 3001 GATGACCACC GATATGGCCA GTGTGCCGGT tTCCGTTATC GGGGAAGAAG TGGCTGATCT 3061 CAGCCACCGC GAAAATGACA TCAAAAACGC CATTAACCTG ATGTTCTGGG GAATATAAAT 3121 GTCAGGCTCC CTTATACACA GCCAGTCggt ctcagcttaa caaaagttga tttctgaaga 3181 agatttgaac ggtgaacaaa agctaatctc cgaggaagac ttgaacggtg aacaaaaatt 3241 aatctcagaa gaagacttga actgaAAGCT TTAAGCGTTA ATATTTTGTT AAAATTCGCG 3301 TTAAATTTTT GTTAAATCAG CTCATTTTTT AACCAATAGG CCGACTGCGA TGAGTGGCAG 3361 GGCGGGGCGT AATTTTTTTA AGGCAGTTAT TGGTGCCCTT AAACGCCTGG TGCTACGCCT 3421 GAATAAGTGA TAATAAGCGG ATGAATGGCA GAAATTCGAA AGCAAATTCG ACCCGGTCGT 3481 CGGTTCAGGG CAGGGTCGTT AAATAGCCGC TTATGTCTAT TGCTGGTTTA CCGGTTTATT 3541 GACTACCGGA AGCAGTGTGA CCGTGTGCTT CTCAAATGCC TGAGGCCAGT TTGCTCAGGC 3601 TCTCCCCGTG GAGGTAATAA TTGACGATAT GATCATTTAT TCTGCCTCCC AGAGCCTGAT 3661 AAAAACGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG 3721 CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GAGGCGGCTA 3781 CAGCCGATAG TCTGGAACAG CGCACTTACG GGTTGCTGCG CAACCCAAGT GCTACCGGCG 3841 CGGCAGCGTG ACCCGTGTCG GCGGCTCCAA CGGCTCGCCA TCGTCCAGAA AACACGGCTC 3901 ATCGGGCATC GGCAGGCGCT GCTGCCCGCG CCGTTCCCAT TCCTCCGTTT CGGTCAAGGC 3961 TGGCAGGTCT GGTTCCATGC CCGGAATGCC GGGCTGGCTG GGCGGCTCCT CGCCGGGGCC 4021 GGTCGGTAGT TGCTGCTCGC CCGGATACAG GGTCGGGATG CGGCGCAGGT CGCCATGCCC 4081 CAACAGCGAT TCGTCCTGGT CGTCGTGATC AACCACCACG GCGGCACTGA ACACCGACAG 4141 GCGCAACTGG TCGCGGGGCT GGCCCCACGC CACGCGGTCA TTGACCACGT AGGCCGACAC 4201 GGTGCCGGGG CCGTTGAGCT TCACGACGGA GATCCAGCGC TCGGCCACCA AGTCCTTGAC 4261 TGCGTATTGG ACCGTCCGCA AAGAACGTCC GATGAGCTTG GAAAGTGTCT TCTGGCTGAC 4321 CACCACGGCG TTCTGGTGGC CCATCTGCGC CACGAGGTGA TGCAGCAGCA TTGCCGCCGT 4381 GGGTTTCCTC GCAATAAGCC CGGCCCACGC CTCATGCGCT TTGCGTTCCG TTTGCACCCA 4441 GTGACCGGGC TTGTTCTTGG CTTGAATGCC GATTTCTCTG GACTGCGTGG CCATGCTTAT 4501 CTCCATGCGG TAGGGTGCCG CACGGTTGCG GCACCATGCG CAATCAGCTG CAACTTTTCG 4561 GCAGCGCGAC AACAATTATG CGTTGCGTAA AAGTGGCAGT CAATTACAGA TTTTCTTTAA 4621 CCTACGCAAT GAGCTATTGC GGGGGGTGCC GCAATGAGCT GTTGCGTACC CCCCTTTTTT 4681 AAGTTGTTGA TTTTTAAGTC TTTCGCATTT CGCCCTATAT CTAGTTCTTT GGTGCCCAAA 4741 GAAGGGCACC CCTGCGGGGT TCCCCCACGC CTTCGGCGCG GCTCCCCCTC CGGCAAAAAG 4801 TGGCCCCTCC GGGGCTTGTT GATCGACTGC GCGGCCTTCG GCCTTGCCCA AGGTGGCGCT 4861 GCCCCCTTGG AACCCCCGCA CTCGCCGCCG TGAGGCTCGG GGGGCAGGCG GGCGGGCTTC 4921 GCCTTCGACT GCCCCCACTC GCATAGGCTT GGGTCGTTCC AGGCGCGTCA AGGCCAAGCC 4981 GCTGCGCGGT CGCTGCGCGA GCCTTGACCC GCCTTCCACT TGGTGTCCAA CCGGCAAGCG 5041 AAGCGCGCAG GCCGCAGGCC GGAGGCTTTT CCCCAGAGAA AATTAAAAAA ATTGATGGGG 5101 CAAGGCCGCA GGCCGCGCAG TTGGAGCCGG TGGGTATGTG GTCGAAGGCT GGGTAGCCGG 5161 TGGGCAATCC CTGTGGTCAA GCTCGTGGGC AGGCGCAGCC TGTCCATCAG CTTGTCCAGC 5221 AGGGTTGTCC ACGGGCCGAG CGAAGCGAGC CAGCCGGTGG CCGCTCGCGG CCATCGTCCA 5281 CATATCCACG GGCTGGCAAG GGAGCGCAGC GACCGCGCAG GGCGAAGCCC GGAGAGCAAG 5341 CCCGTAGGGC GCCGCAGCCG CCGTAGGCGG TCACGACTTT GCGAAGCAAA GTCTAGTGAG 5401 TATACTCAAG CATTGAGTGG CCCGCCGGAG GCACCGCCTT GCGCTGCCCC CGTCGAGCCG 5461 GTTGGACACC AAAAGGGAGG GGCAGGCATG GCGGCATACG CGATCATGCG ATGCAAGAAG 5521 CTGGCGAAAA TGGGCAACGT GGCGGCCAGT CTCAAGCACG CCTACCGCGA GCGCGAGACG 5581 CCCAACGCTG ACGCCAGCAG GACGCCAGAG AACGAGCACT GGGCGGCCAG CAGCACCGAT 5641 GAAGCGATGG GCCGACTGCG CGAGTTGCTG CCAGAGAAGC GGCGCAAGGA CGCTGTGTTG 5701 GCGGTCGAGT ACGTCATGAC GGCCAGCCCG GAATGGTGGA AGTCGGCCAG CCAAGAACAG 5761 CAGGCGGCGT TCTTCGAGAA GGCGCACAAG TGGCTGGCGG ACAAGTACGG GGCGGATCGC 5821 ATCGTGACGG CCAGCATCCA CCGTGACGAA ACCAGCCCGC ACATGACCGC GTTCGTGGTG 5881 CCGCTGACGC AGGACGGCAG GCTGTCGGCC AAGGAGTTCA TCGGCAACAA AGCGCAGATG 5941 ACCCGCGACC AGACCACGTT TGCGGCCGCT GTGGCCGATC TAGGGCTGCA ACGGGGCATC 6001 GAGGGCAGCA AGGCACGTCA CACGCGCATT CAGGCGTTCT ACGAGGCCCT GGAGCGGCCA 6061 CCAGTGGGCC ACGTCACCAT CAGCCCGCAA GCGGTCGAGC CACGCGCCTA TGCACCGCAG 6121 GGATTGGCCG AAAAGCTGGG AATCTCAAAG CGCGTTGAGA CGCCGGAAGC CGTGGCCGAC 6181 CGGCTGACAA AAGCGGTTCG GCAGGGGTAT GAGCCTGCCC TACAGGCCGC CGCAGGAGCG 6241 CGTGAGATGC GCAAGAAGGC CGATCAAGCC CAAGAGACGG CCCGAgATCT TCGGGAGCGC 6301 CTGAAGCCCG TTCTGGACGC CCTGGGGCCG TTGAATCGGG ATATGCAGGC CAAGGCCGCC 6361 GCGATCATCA AGGCCGTGGG CGAAAAGCT //