LOCUS pJOG317_[CT]_4XMyc-TwinStrep 2469 bp DNA circular UNA 23-OCT-2012 COMMENT This file is created by Vector NTI http://www.invitrogen.com/ FEATURES Location/Qualifiers misc_feature join(1973..2469,1..118) /vntifkey="21" /label="rep (pMB1)" rep_origin complement(103) /vntifkey="33" /label=ORI /note="RNaseH cleavage point" primer 211..230 /label=JS836 MIsc._Feature <351..354 /label="TTCG4bp overhang" CDS 355..474 /label="C terminal 4xMyc tag" CDS 481..>572 /label="Twin Strep-tag" misc_feature 573..576 /vntifkey="21" /label=GCTT primer complement(690..707) /label=JS837 CDS 803..1813 /vntifkey="4" /label="Sm/Sp no DraIII" mutation 1780 /vntifkey="62" /label="G to A" ORIGIN 1 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG 61 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC 121 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC 181 CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG 241 CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA 301 ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATCACTC TGTGGTCTCA TTCGGAACAA 361 AAGTTGATCT CTGAAGAGGA CCTTGAGCAG AAATTGATCT CTGAGGAAGA TCTTGAGCAG 421 AAGCTTATCT CAGAGGAGGA TTTGGAGCAA AAACTTATTT CTGAAGAGGA CCTTgcCGCT 481 TCTGCTTGGT CACACCCACA GTTCGAGAAG GGAGGTGGAT CTGGTGGAGG TTCAGGAGGA 541 TCAGCTTGGT CACATCCTCA ATTTGAAAAA TAGCTTTGAG ACCACGAAGT GGCTCTTCAG 601 TGGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT 661 TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT 721 TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA 781 ATAATATTGA AAAAGGAAGA GTATGCGCTC ACGCAACTGG TCCAGAACCT TGACCGAACG 841 CAGCGGTGGT AACGGCGCAG TGGCGGTTTT CATGGCTTGT TATGACTGTT TTTTTGGGGT 901 ACAGTCTATG CCTCGGGCAT CCAAGCAGCA AGCGCGTTAC GCCGTGGGTC GATGTTTGAT 961 GTTATGGAGC AGCAACGATG TTACGCAGCA GGGCAGTCGC CCTAAAACAA AGTTAAACAT 1021 CATGAGGGAA GCGGTGATCG CCGAAGTATC GACTCAACTA TCAGAGGTAG TTGGCGTCAT 1081 CGAGCGCCAT CTCGAACCGA CGTTGCTGGC CGTACATTTG TACGGCTCCG CAGTGGATGG 1141 CGGCCTGAAG CCACACAGCG ATATTGATTT GCTGGTTACG GTGACCGTAA GGCTTGATGA 1201 AACAACGCGG CGAGCTTTGA TCAACGACCT TTTGGAAACT TCGGCTTCCC CTGGAGAGAG 1261 CGAGATTCTC CGCGCTGTAG AAGTCACCAT TGTTGTGCAC GACGACATCA TTCCGTGGCG 1321 TTATCCAGCT AAGCGCGAAC TGCAATTTGG AGAATGGCAG CGCAATGACA TTCTTGCAGG 1381 TATCTTCGAG CCAGCCACGA TCGACATTGA TCTGGCTATC TTGCTGACAA AAGCAAGAGA 1441 ACATAGCGTT GCCTTGGTAG GTCCAGCGGC GGAGGAACTC TTTGATCCGG TTCCTGAACA 1501 GGATCTATTT GAGGCGCTAA ATGAAACCTT AACGCTATGG AACTCGCCGC CCGACTGGGC 1561 TGGCGATGAG CGAAATGTAG TGCTTACGTT GTCCCGCATT TGGTACAGCG CAGTAACCGG 1621 CAAAATCGCG CCGAAGGATG TCGCTGCCGA CTGGGCAATG GAGCGCCTGC CGGCCCAGTA 1681 TCAGCCCGTC ATACTTGAAG CTAGACAGGC TTATCTTGGA CAAGAAGAAG ATCGCTTGGC 1741 CTCGCGCGCA GATCAGTTGG AAGAATTTGT CCATTACGTA AAAGGCGAGA TCACCAAGGT 1801 AGTCGGCAAA TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT 1861 TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT 1921 CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC 1981 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT 2041 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG 2101 CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA 2161 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC 2221 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA 2281 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC 2341 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA 2401 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG 2461 GGAGCTTCC //