LOCUS Exported 7921 bp ds-DNA circular SYN 05-DEC-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Tol2-lyzC-miR-16a-dendra SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7921) AUTHORS . TITLE Direct Submission JOURNAL Exported Tuesday, Dec 5, 2017 from SnapGene 3.1.3 http://www.snapgene.com COMMENT [DEFINITION]: [LOCUS]: Construct_1 [ACCESSION]: pVec1 FEATURES Location/Qualifiers source 1..7921 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..15 /note="aptemer-" misc_feature 7544..7558 /note="aptemer+" misc_feature 7559..7921 /note="miR16" misc_feature 7876 /note="deletion" ORIGIN 1 agccaactgc tgttactctc gcccagaaat ctgcgccaac atggacacac ccagtggagc 61 aagccatctg attttcatgg tcagattgta aagaagcaat tactcttgga cagtttaagc 121 agaaaagcca ccaagtcaag tcactggtca tatgatgaac tcgaagatct aataaaagtt 181 aatttaagcg ttcattacaa taaattcaag tgaatctgtt ttaagctagc ctttaacgtt 241 tttaaattcc ttcaaacaaa ttggatcctc tttgcagttc ctcatttctc ggtgagctgg 301 tgctttaatc cctttacttt ttcttcctct agggagtgat ggttggcatg ggacgaatta 361 attcctgcag cccgggggta ccggtcgcca ccatgaacac cccgggaatt aacctgatca 421 aggaggacat gcgcgtgaag gtgcacatgg agggcaacgt gaacggccac gccttcgtga 481 tcgagggcga gggcaagggc aagccctacg agggcaccca gaccgccaac ctgaccgtga 541 aggagggcgc ccccctgccc ttcagctacg acatcctgac caccgccgtg cactacggca 601 accgggtgtt caccaagtac cccgaggaca tccccgacta cttcaagcag agcttccccg 661 agggctacag ctgggagcgc accatgacct tcgaggacaa gggcatctgc accatccgca 721 gcgacatcag cctggagggc gactgcttct tccagaacgt gcgcttcaag ggcaccaact 781 tcccccccaa cggccccgtg atgcagaaga aaaccctgaa gtgggagccc agcaccgaga 841 agctgcacgt gcgcgacggc ctgctggtgg gcaacatcaa catggccctg ctgctggagg 901 gcggcggcca ctacctgtgc gacttcaaga ccacctacaa ggccaagaag gtggtgcagc 961 tgcccgacgc ccacttcgtg gaccaccgca tcgagatcct gggcaacgac agcgactaca 1021 acaaggtgaa gctgtacgag cacgccgtgg cccgctacag ccccctgccc agccaggtgt 1081 ggtaatctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa 1141 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt 1201 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 1261 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg 1321 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 1381 cattttttaa ccaataggcc gaaatcggcg gatcctgcgg ccgcaaggga aaatagaatg 1441 aagtgatctc caaaaaataa gtactttttg actgtaaata aaattgtaag gagtaaaaag 1501 tacttttttt tctaaaaaaa tgtaattaag taaaagtaaa agtattgatt tttaattgta 1561 ctcaagtaaa gtaaaaatcc ccaaaaataa tacttaagta cagtaatcaa gtaaaattac 1621 tcaagtactt tacacctctg gttcttgacc ccctaccttc agcaagccca gcagatccac 1681 tagatctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag 1741 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 1801 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 1861 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 1921 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 1981 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 2041 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 2101 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 2161 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 2221 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 2281 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 2341 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 2401 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 2461 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 2521 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 2581 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 2641 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 2701 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 2761 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 2821 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 2881 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 2941 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 3001 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 3061 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 3121 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 3181 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 3241 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 3301 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 3361 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 3421 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 3481 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 3541 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 3601 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 3661 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 3721 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 3781 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 3841 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta 3901 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg 3961 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg 4021 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa 4081 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg 4141 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt 4201 gacggggaaa gccggccaca atgacggcta ctacatggtg ccattccttc ctctttatag 4261 gaatggagac tacctcctgt ccaacaaggc tcttggatac gagtacgcct acctgttgga 4321 cccaggtcat tgcacaacac cagaaatgcc ctctgatctg caaaagacgt gaatatctgt 4381 tcagacaccc atatccactc tgttccacac aggtcagagg tttgtccagg agttcttgac 4441 agaggtgtaa aaagtactca aaaattttac tcaagtgaaa gtacaagtac ttagggaaaa 4501 ttttactcaa ttaaaagtaa aagtatctgg ctagaatctt acttgagtaa aagtaaaaaa 4561 gtactccatt aaaattgtac ttgagtatta aggaagtaaa aggctagcaa gatctgctcg 4621 agagtttgac ttgggttgat tatagcaaat agtggaacac aaacagattt tgaaatattc 4681 ttgcaaattt atttattaaa tccattcaaa tattgtcatt ttttgtactg tgtaatttat 4741 attgatttgc tcatacaata tttacatttc gcttaaacaa tacactatta tttatattta 4801 aaaatagcta tatataaatc aaatgtatag ctctttttta cacaattaat caaagctaaa 4861 atatttgtca cacaaataca gtaagccact tcacaatgat atcttcaaaa tctgtgcttg 4921 actgaatcat ttcatttatt gttttgtaat tcacattata ccttacgttc tccaggcaca 4981 gtttgaatta ttgataaatg aagataatac atctgattta attagtctcc tttgtttata 5041 ataactttaa tctaatttga tagtaataat tccagcaata catccattac aataaaaatc 5101 gctttttaat tttgcacatt agtttgccgg tatttgttta aagtttcacc atacttttaa 5161 atcatgtgct atttgaaaag ggctgttatg actggaatta aatgtttttt tttttatatc 5221 agtgttttag attgaagtac aatatttata ggtaaaattc accagcatat gcttaaagat 5281 agccatgctc caggtccttg tgtccttcta aggtctgttt gtggtttaag ctgtttgttg 5341 acacacgctt gtcatctcca tttttctgtg cagttattgt cagtttattg cacactgggt 5401 tgcattaagc tgcagttttt tcacaacagt tttccacatt gtaacagtca gtctccaaat 5461 tattccatca gtcctcaaat ttagtcattt gaggccatga aaacacttaa aactacataa 5521 aggtagtttg gcattaggtt ttagacttgt tcttgtttgg caatattgca acctgtgtaa 5581 aataatttga ttcttttttt tttttttttt tttttaattt tcagaaagaa atggtccaaa 5641 aggagctatg tgaacattta tccatgtgtt taatgttttt ttttttatta ttaaaccact 5701 tctgacttaa ctttttttag ttattctatt ttgtgacgag tctgaatggc caaagtactt 5761 ttgaatccaa aatcttacta gtaaaatgtt aaatcttact agcagtgttg ggtataattt 5821 gtaacaaagt taaattccat atctgctaag tgaaaaagta aagtaagaga ttgctgtaaa 5881 tatttaggta atttgtttta ttaatacaat atagaaagta acaaaacaca atgtaaaaaa 5941 atcaataaag aaagtaaata ataaaagtaa tgataaacta atgatctaaa caaaattagt 6001 aataaaattt atacaattca gggagggtaa agaatttcac ctaatagtca aactctgtat 6061 taaataatat aaaatatttg ttcttagtct gaagaggaga ctgtgagaga aagcttactg 6121 gttattaaat tagaaaattt gtaacttgca gattaaagct gtgtgccttg cagattaaaa 6181 taatttaaat aatttaataa aggccatgtt tgagacaaat gtaaaaacat aataaatgtt 6241 aagaatttca gatattgaga ttttacaatt taaaaaaagc aataaagtaa tagcagagac 6301 ctacactctt aaagtaaatt actcaacact gcttactagt aacttttgga atagttacta 6361 gtatccattg aacaaatact agaatctaat taaattgtgt gagtgtccaa tgaaaagatt 6421 cttgtattta aaacagtacc agtatctata tatgtacaaa atttagtgct agcaaaatgc 6481 aaataaatac tactgcatat aatggattgc attgtaaatc gacttgccat aatacattta 6541 tttcctattt ttacttttaa caaaaaataa gttcactgag tatttcatgt cattaaatcg 6601 cattaaacct cttgcatgct ttatgtacac tagtgcagtt cgctaaatga ccaccggtgt 6661 cactaataac acgatcgttt ctgaattctg cattaaacgc catttatacg ttgctcttat 6721 tagtaaacaa tttctccatc atgagctcga ccagtattgc atatttcatc ctcttaaatc 6781 cagagttgtt tcttttaatc actttctctt catgataaaa gcagtttttg gttttcagaa 6841 gtttacaaca aacccagtca gttattgcct cagcaaacac tggagcaact gcacacttgt 6901 gaaaagcttc ttgcgacaca aacctttctc tggacttata aataaaggca ttcagcagct 6961 ccaccataca cctccactgg tcttgataaa gcagatatca gcagtgatac aggtaccgcc 7021 accatggatg aggaaatcgc tgccctggtc gttgacaacg gctccggtat gtgcaaagcc 7081 ggttttgctg gagatgatgc ccctcgtgct gttttcccct ccattgttgg acgacccaga 7141 catcaggtac atttaataaa ccatcctaat aacatgttaa tcatattgaa ccacattaaa 7201 ctctcatttg tgcattttac atcttttaaa accgatttat attcgttttt agataatgaa 7261 gtttattaaa gtgttaattc acagctttat tgcctgtttt gagaatctgc ttgcataagt 7321 gatttttaaa cttgatctga ttctcgtttg tttcgaagtt cttcagcttt gaaagtcaga 7381 tcttcattca cagccatgcc atagttttag gatacagtat aagtagacga attgggatgc 7441 atcgtagatt ttatgctgtg tttgttatgg tttttcagtg gtagagattt tcagagtatt 7501 attagggtta tagtcagtgt caggtttatt tatcgtcagc gtgatgggca aaaaacagtg 7561 caagccatca tttgctgctc tagagtttta aggcattctc ctacactaaa cctgacactt 7621 gcttgaggtt tgtaaacatg ggctgtccga aaaggtcaga ggtcaacatc tgttcattct 7681 accttcctcg ctttagcagc acgtaaatat tggtgtgtta tagtcaaggc caaccccaat 7741 attatgtgtg ctgcttcagt aaggcaggag cgagcctcct ttcacttcct gtacatcatt 7801 attcactttg gtcaatcaca tttcctgtgt gctggtgcac aaataaaaat aaatctgttt 7861 tgttcttgtt tgttttgaac tgtgcataca atttaatgct acatcggttt cattctgcac 7921 t //