LOCUS Exported 7859 bp ds-DNA circular SYN 05-DEC-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Tol2-lyzC-miR-23a-2-3p-dendra SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7859) AUTHORS . TITLE Direct Submission JOURNAL Exported Tuesday, Dec 5, 2017 from SnapGene 3.1.3 http://www.snapgene.com COMMENT [DEFINITION]: [LOCUS]: Construct_1 [ACCESSION]: pVec1 FEATURES Location/Qualifiers source 1..7859 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 3536..3565 /note="intron primer" misc_feature 3680..3714 /note="miR-23a+" misc_feature 3958..3995 /note="miR-23a-" misc_feature 4373..5071 /note="dendra2" misc_feature 5045..5062 /note="dendra-primer" primer_bind complement(5673..5692) /note="T3" primer_bind complement(5710..5730) /note="M13-rev" misc_binding complement(5736..5758) /note="LacO" promoter complement(5763..5792) /note="lac" rep_origin complement(6098..6726) /direction=LEFT /note="ColE1 origin" CDS complement(6878..7537) /note="AmpR" promoter complement(7777..7805) /note="Amp prom" ORIGIN 1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga 121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 301 ggagcccccg atttagagct tgacggggaa agccggccac aatgacggct actacatggt 361 gccattcctt cctctttata ggaatggaga ctacctcctg tccaacaagg ctcttggata 421 cgagtacgcc tacctgttgg acccaggtca ttgcacaaca ccagaaatgc cctctgatct 481 gcaaaagacg tgaatatctg ttcagacacc catatccact ctgttccaca caggtcagag 541 gtttgtccag gagttcttga cagaggtgta aaaagtactc aaaaatttta ctcaagtgaa 601 agtacaagta cttagggaaa attttactca attaaaagta aaagtatctg gctagaatct 661 tacttgagta aaagtaaaaa agtactccat taaaattgta cttgagtatt aaggaagtaa 721 aaggctagca agatctgctc gagagtttga cttgggttga ttatagcaaa tagtggaaca 781 caaacagatt ttgaaatatt cttgcaaatt tatttattaa atccattcaa atattgtcat 841 tttttgtact gtgtaattta tattgatttg ctcatacaat atttacattt cgcttaaaca 901 atacactatt atttatattt aaaaatagct atatataaat caaatgtata gctctttttt 961 acacaattaa tcaaagctaa aatatttgtc acacaaatac agtaagccac ttcacaatga 1021 tatcttcaaa atctgtgctt gactgaatca tttcatttat tgttttgtaa ttcacattat 1081 accttacgtt ctccaggcac agtttgaatt attgataaat gaagataata catctgattt 1141 aattagtctc ctttgtttat aataacttta atctaatttg atagtaataa ttccagcaat 1201 acatccatta caataaaaat cgctttttaa ttttgcacat tagtttgccg gtatttgttt 1261 aaagtttcac catactttta aatcatgtgc tatttgaaaa gggctgttat gactggaatt 1321 aaatgttttt ttttttatat cagtgtttta gattgaagta caatatttat aggtaaaatt 1381 caccagcata tgcttaaaga tagccatgct ccaggtcctt gtgtccttct aaggtctgtt 1441 tgtggtttaa gctgtttgtt gacacacgct tgtcatctcc atttttctgt gcagttattg 1501 tcagtttatt gcacactggg ttgcattaag ctgcagtttt ttcacaacag ttttccacat 1561 tgtaacagtc agtctccaaa ttattccatc agtcctcaaa tttagtcatt tgaggccatg 1621 aaaacactta aaactacata aaggtagttt ggcattaggt tttagacttg ttcttgtttg 1681 gcaatattgc aacctgtgta aaataatttg attctttttt tttttttttt ttttttaatt 1741 ttcagaaaga aatggtccaa aaggagctat gtgaacattt atccatgtgt ttaatgtttt 1801 tttttttatt attaaaccac ttctgactta acttttttta gttattctat tttgtgacga 1861 gtctgaatgg ccaaagtact tttgaatcca aaatcttact agtaaaatgt taaatcttac 1921 tagcagtgtt gggtataatt tgtaacaaag ttaaattcca tatctgctaa gtgaaaaagt 1981 aaagtaagag attgctgtaa atatttaggt aatttgtttt attaatacaa tatagaaagt 2041 aacaaaacac aatgtaaaaa aatcaataaa gaaagtaaat aataaaagta atgataaact 2101 aatgatctaa acaaaattag taataaaatt tatacaattc agggagggta aagaatttca 2161 cctaatagtc aaactctgta ttaaataata taaaatattt gttcttagtc tgaagaggag 2221 actgtgagag aaagcttact ggttattaaa ttagaaaatt tgtaacttgc agattaaagc 2281 tgtgtgcctt gcagattaaa ataatttaaa taatttaata aaggccatgt ttgagacaaa 2341 tgtaaaaaca taataaatgt taagaatttc agatattgag attttacaat ttaaaaaaag 2401 caataaagta atagcagaga cctacactct taaagtaaat tactcaacac tgcttactag 2461 taacttttgg aatagttact agtatccatt gaacaaatac tagaatctaa ttaaattgtg 2521 tgagtgtcca atgaaaagat tcttgtattt aaaacagtac cagtatctat atatgtacaa 2581 aatttagtgc tagcaaaatg caaataaata ctactgcata taatggattg cattgtaaat 2641 cgacttgcca taatacattt atttcctatt tttactttta acaaaaaata agttcactga 2701 gtatttcatg tcattaaatc gcattaaacc tcttgcatgc tttatgtaca ctagtgcagt 2761 tcgctaaatg accaccggtg tcactaataa cacgatcgtt tctgaattct gcattaaacg 2821 ccatttatac gttgctctta ttagtaaaca atttctccat catgagctcg accagtattg 2881 catatttcat cctcttaaat ccagagttgt ttcttttaat cactttctct tcatgataaa 2941 agcagttttt ggttttcaga agtttacaac aaacccagtc agttattgcc tcagcaaaca 3001 ctggagcaac tgcacacttg tgaaaagctt cttgcgacac aaacctttct ctggacttat 3061 aaataaaggc attcagcagc tccaccatac acctccactg gtcttgataa agcagatatc 3121 agcagtgata caggtaccgc caccatggat gaggaaatcg ctgccctggt cgttgacaac 3181 ggctccggta tgtgcaaagc cggttttgct ggagatgatg cccctcgtgc tgttttcccc 3241 tccattgttg gacgacccag acatcaggta catttaataa accatcctaa taacatgtta 3301 atcatattga accacattaa actctcattt gtgcatttta catcttttaa aaccgattta 3361 tattcgtttt tagataatga agtttattaa agtgttaatt cacagcttta ttgcctgttt 3421 tgagaatctg cttgcataag tgatttttaa acttgatctg attctcgttt gtttcgaagt 3481 tcttcagctt tgaaagtcag atcttcattc acagccatgc catagtttta ggatacagta 3541 taagtagacg aattgggatg catcgtagat tttatgctgt gtttgttatg gtttttcagt 3601 ggtagagatt ttcagagtat tattagggtt atagtcagtg tcaggtttat ttatcgtcag 3661 cgtgatgggc aaaaaacagc tggcagtacg ggctatatag ccgtgtcggt ccctcagaca 3721 cagcgactta aatccagacc ctcctcttct gccggccagg ggaattcctg gcagagtgat 3781 ttttaaacct aatgactgaa tcacattgcc agggatttcc aatggctcgt gtgactcaga 3841 gacacgccag atataaatac tcggcaaaca cacacacacc tgaacacatg agaagctaat 3901 cctaaaggaa tgccgactgt ctcgcacact agttagcggt gtcaggaatt catgtgcact 3961 tctatgcggg ttgtgtggtg agccaactgc tgttactctc gcccagaaat ctgcgccaac 4021 atggacacac ccagtggagc aagccatctg attttcatgg tcagattgta aagaagcaat 4081 tactcttgga cagtttaagc agaaaagcca ccaagtcaag tcactggtca tatgatgaac 4141 tcgaagatct aataaaagtt aatttaagcg ttcattacaa taaattcaag tgaatctgtt 4201 ttaagctagc ctttaacgtt tttaaattcc ttcaaacaaa ttggatcctc tttgcagttc 4261 ctcatttctc ggtgagctgg tgctttaatc cctttacttt ttcttcctct agggagtgat 4321 ggttggcatg ggacgaatta attcctgcag cccgggggta ccggtcgcca ccatgaacac 4381 cccgggaatt aacctgatca aggaggacat gcgcgtgaag gtgcacatgg agggcaacgt 4441 gaacggccac gccttcgtga tcgagggcga gggcaagggc aagccctacg agggcaccca 4501 gaccgccaac ctgaccgtga aggagggcgc ccccctgccc ttcagctacg acatcctgac 4561 caccgccgtg cactacggca accgggtgtt caccaagtac cccgaggaca tccccgacta 4621 cttcaagcag agcttccccg agggctacag ctgggagcgc accatgacct tcgaggacaa 4681 gggcatctgc accatccgca gcgacatcag cctggagggc gactgcttct tccagaacgt 4741 gcgcttcaag ggcaccaact tcccccccaa cggccccgtg atgcagaaga aaaccctgaa 4801 gtgggagccc agcaccgaga agctgcacgt gcgcgacggc ctgctggtgg gcaacatcaa 4861 catggccctg ctgctggagg gcggcggcca ctacctgtgc gacttcaaga ccacctacaa 4921 ggccaagaag gtggtgcagc tgcccgacgc ccacttcgtg gaccaccgca tcgagatcct 4981 gggcaacgac agcgactaca acaaggtgaa gctgtacgag cacgccgtgg cccgctacag 5041 ccccctgccc agccaggtgt ggtaatctag atcataatca gccataccac atttgtagag 5101 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat 5161 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc 5221 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 5281 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt 5341 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggcg gatcctgcgg 5401 ccgcaaggga aaatagaatg aagtgatctc caaaaaataa gtactttttg actgtaaata 5461 aaattgtaag gagtaaaaag tacttttttt tctaaaaaaa tgtaattaag taaaagtaaa 5521 agtattgatt tttaattgta ctcaagtaaa gtaaaaatcc ccaaaaataa tacttaagta 5581 cagtaatcaa gtaaaattac tcaagtactt tacacctctg gttcttgacc ccctaccttc 5641 agcaagccca gcagatccac tagatctttt gttcccttta gtgagggtta attgcgcgct 5701 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 5761 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 5821 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 5881 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 5941 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 6001 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 6061 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 6121 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 6181 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 6241 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 6301 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 6361 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 6421 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 6481 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 6541 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 6601 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 6661 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 6721 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 6781 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 6841 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 6901 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 6961 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 7021 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 7081 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 7141 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 7201 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 7261 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 7321 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 7381 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 7441 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 7501 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 7561 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 7621 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 7681 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 7741 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 7801 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtg //