LOCUS       Exported                7859 bp ds-DNA     circular SYN 05-DEC-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Tol2-lyzC-miR-23a-2-3p-dendra
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 5, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
COMMENT     [DEFINITION]: [LOCUS]: Construct_1 [ACCESSION]: pVec1
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    3536..3565
                     /note="intron primer"
     misc_feature    3680..3714
                     /note="miR-23a+"
     misc_feature    3958..3995
                     /note="miR-23a-"
     misc_feature    4373..5071
                     /note="dendra2"
     misc_feature    5045..5062
                     /note="dendra-primer"
     primer_bind     complement(5673..5692)
                     /note="T3"
     primer_bind     complement(5710..5730)
                     /note="M13-rev"
     misc_binding    complement(5736..5758)
                     /note="LacO"
     promoter        complement(5763..5792)
                     /note="lac"
     rep_origin      complement(6098..6726)
                     /direction=LEFT
                     /note="ColE1 origin"
     CDS             complement(6878..7537)
                     /note="AmpR"
     promoter        complement(7777..7805)
                     /note="Amp prom"
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggccac aatgacggct actacatggt
      361 gccattcctt cctctttata ggaatggaga ctacctcctg tccaacaagg ctcttggata
      421 cgagtacgcc tacctgttgg acccaggtca ttgcacaaca ccagaaatgc cctctgatct
      481 gcaaaagacg tgaatatctg ttcagacacc catatccact ctgttccaca caggtcagag
      541 gtttgtccag gagttcttga cagaggtgta aaaagtactc aaaaatttta ctcaagtgaa
      601 agtacaagta cttagggaaa attttactca attaaaagta aaagtatctg gctagaatct
      661 tacttgagta aaagtaaaaa agtactccat taaaattgta cttgagtatt aaggaagtaa
      721 aaggctagca agatctgctc gagagtttga cttgggttga ttatagcaaa tagtggaaca
      781 caaacagatt ttgaaatatt cttgcaaatt tatttattaa atccattcaa atattgtcat
      841 tttttgtact gtgtaattta tattgatttg ctcatacaat atttacattt cgcttaaaca
      901 atacactatt atttatattt aaaaatagct atatataaat caaatgtata gctctttttt
      961 acacaattaa tcaaagctaa aatatttgtc acacaaatac agtaagccac ttcacaatga
     1021 tatcttcaaa atctgtgctt gactgaatca tttcatttat tgttttgtaa ttcacattat
     1081 accttacgtt ctccaggcac agtttgaatt attgataaat gaagataata catctgattt
     1141 aattagtctc ctttgtttat aataacttta atctaatttg atagtaataa ttccagcaat
     1201 acatccatta caataaaaat cgctttttaa ttttgcacat tagtttgccg gtatttgttt
     1261 aaagtttcac catactttta aatcatgtgc tatttgaaaa gggctgttat gactggaatt
     1321 aaatgttttt ttttttatat cagtgtttta gattgaagta caatatttat aggtaaaatt
     1381 caccagcata tgcttaaaga tagccatgct ccaggtcctt gtgtccttct aaggtctgtt
     1441 tgtggtttaa gctgtttgtt gacacacgct tgtcatctcc atttttctgt gcagttattg
     1501 tcagtttatt gcacactggg ttgcattaag ctgcagtttt ttcacaacag ttttccacat
     1561 tgtaacagtc agtctccaaa ttattccatc agtcctcaaa tttagtcatt tgaggccatg
     1621 aaaacactta aaactacata aaggtagttt ggcattaggt tttagacttg ttcttgtttg
     1681 gcaatattgc aacctgtgta aaataatttg attctttttt tttttttttt ttttttaatt
     1741 ttcagaaaga aatggtccaa aaggagctat gtgaacattt atccatgtgt ttaatgtttt
     1801 tttttttatt attaaaccac ttctgactta acttttttta gttattctat tttgtgacga
     1861 gtctgaatgg ccaaagtact tttgaatcca aaatcttact agtaaaatgt taaatcttac
     1921 tagcagtgtt gggtataatt tgtaacaaag ttaaattcca tatctgctaa gtgaaaaagt
     1981 aaagtaagag attgctgtaa atatttaggt aatttgtttt attaatacaa tatagaaagt
     2041 aacaaaacac aatgtaaaaa aatcaataaa gaaagtaaat aataaaagta atgataaact
     2101 aatgatctaa acaaaattag taataaaatt tatacaattc agggagggta aagaatttca
     2161 cctaatagtc aaactctgta ttaaataata taaaatattt gttcttagtc tgaagaggag
     2221 actgtgagag aaagcttact ggttattaaa ttagaaaatt tgtaacttgc agattaaagc
     2281 tgtgtgcctt gcagattaaa ataatttaaa taatttaata aaggccatgt ttgagacaaa
     2341 tgtaaaaaca taataaatgt taagaatttc agatattgag attttacaat ttaaaaaaag
     2401 caataaagta atagcagaga cctacactct taaagtaaat tactcaacac tgcttactag
     2461 taacttttgg aatagttact agtatccatt gaacaaatac tagaatctaa ttaaattgtg
     2521 tgagtgtcca atgaaaagat tcttgtattt aaaacagtac cagtatctat atatgtacaa
     2581 aatttagtgc tagcaaaatg caaataaata ctactgcata taatggattg cattgtaaat
     2641 cgacttgcca taatacattt atttcctatt tttactttta acaaaaaata agttcactga
     2701 gtatttcatg tcattaaatc gcattaaacc tcttgcatgc tttatgtaca ctagtgcagt
     2761 tcgctaaatg accaccggtg tcactaataa cacgatcgtt tctgaattct gcattaaacg
     2821 ccatttatac gttgctctta ttagtaaaca atttctccat catgagctcg accagtattg
     2881 catatttcat cctcttaaat ccagagttgt ttcttttaat cactttctct tcatgataaa
     2941 agcagttttt ggttttcaga agtttacaac aaacccagtc agttattgcc tcagcaaaca
     3001 ctggagcaac tgcacacttg tgaaaagctt cttgcgacac aaacctttct ctggacttat
     3061 aaataaaggc attcagcagc tccaccatac acctccactg gtcttgataa agcagatatc
     3121 agcagtgata caggtaccgc caccatggat gaggaaatcg ctgccctggt cgttgacaac
     3181 ggctccggta tgtgcaaagc cggttttgct ggagatgatg cccctcgtgc tgttttcccc
     3241 tccattgttg gacgacccag acatcaggta catttaataa accatcctaa taacatgtta
     3301 atcatattga accacattaa actctcattt gtgcatttta catcttttaa aaccgattta
     3361 tattcgtttt tagataatga agtttattaa agtgttaatt cacagcttta ttgcctgttt
     3421 tgagaatctg cttgcataag tgatttttaa acttgatctg attctcgttt gtttcgaagt
     3481 tcttcagctt tgaaagtcag atcttcattc acagccatgc catagtttta ggatacagta
     3541 taagtagacg aattgggatg catcgtagat tttatgctgt gtttgttatg gtttttcagt
     3601 ggtagagatt ttcagagtat tattagggtt atagtcagtg tcaggtttat ttatcgtcag
     3661 cgtgatgggc aaaaaacagc tggcagtacg ggctatatag ccgtgtcggt ccctcagaca
     3721 cagcgactta aatccagacc ctcctcttct gccggccagg ggaattcctg gcagagtgat
     3781 ttttaaacct aatgactgaa tcacattgcc agggatttcc aatggctcgt gtgactcaga
     3841 gacacgccag atataaatac tcggcaaaca cacacacacc tgaacacatg agaagctaat
     3901 cctaaaggaa tgccgactgt ctcgcacact agttagcggt gtcaggaatt catgtgcact
     3961 tctatgcggg ttgtgtggtg agccaactgc tgttactctc gcccagaaat ctgcgccaac
     4021 atggacacac ccagtggagc aagccatctg attttcatgg tcagattgta aagaagcaat
     4081 tactcttgga cagtttaagc agaaaagcca ccaagtcaag tcactggtca tatgatgaac
     4141 tcgaagatct aataaaagtt aatttaagcg ttcattacaa taaattcaag tgaatctgtt
     4201 ttaagctagc ctttaacgtt tttaaattcc ttcaaacaaa ttggatcctc tttgcagttc
     4261 ctcatttctc ggtgagctgg tgctttaatc cctttacttt ttcttcctct agggagtgat
     4321 ggttggcatg ggacgaatta attcctgcag cccgggggta ccggtcgcca ccatgaacac
     4381 cccgggaatt aacctgatca aggaggacat gcgcgtgaag gtgcacatgg agggcaacgt
     4441 gaacggccac gccttcgtga tcgagggcga gggcaagggc aagccctacg agggcaccca
     4501 gaccgccaac ctgaccgtga aggagggcgc ccccctgccc ttcagctacg acatcctgac
     4561 caccgccgtg cactacggca accgggtgtt caccaagtac cccgaggaca tccccgacta
     4621 cttcaagcag agcttccccg agggctacag ctgggagcgc accatgacct tcgaggacaa
     4681 gggcatctgc accatccgca gcgacatcag cctggagggc gactgcttct tccagaacgt
     4741 gcgcttcaag ggcaccaact tcccccccaa cggccccgtg atgcagaaga aaaccctgaa
     4801 gtgggagccc agcaccgaga agctgcacgt gcgcgacggc ctgctggtgg gcaacatcaa
     4861 catggccctg ctgctggagg gcggcggcca ctacctgtgc gacttcaaga ccacctacaa
     4921 ggccaagaag gtggtgcagc tgcccgacgc ccacttcgtg gaccaccgca tcgagatcct
     4981 gggcaacgac agcgactaca acaaggtgaa gctgtacgag cacgccgtgg cccgctacag
     5041 ccccctgccc agccaggtgt ggtaatctag atcataatca gccataccac atttgtagag
     5101 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     5161 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     5221 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5281 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     5341 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggcg gatcctgcgg
     5401 ccgcaaggga aaatagaatg aagtgatctc caaaaaataa gtactttttg actgtaaata
     5461 aaattgtaag gagtaaaaag tacttttttt tctaaaaaaa tgtaattaag taaaagtaaa
     5521 agtattgatt tttaattgta ctcaagtaaa gtaaaaatcc ccaaaaataa tacttaagta
     5581 cagtaatcaa gtaaaattac tcaagtactt tacacctctg gttcttgacc ccctaccttc
     5641 agcaagccca gcagatccac tagatctttt gttcccttta gtgagggtta attgcgcgct
     5701 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     5761 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     5821 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     5881 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     5941 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     6001 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6061 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6121 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6181 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6241 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6301 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6361 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6421 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6481 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6541 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6601 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6661 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6721 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6781 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     6841 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     6901 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6961 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7021 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7081 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7141 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7201 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7261 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7321 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7381 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7441 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7501 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7561 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7621 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7681 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     7741 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     7801 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtg
//
