LOCUS       Exported                7905 bp ds-DNA     circular SYN 05-DEC-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Tol2-lyzC-miR-27b-dendra
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 5, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
COMMENT     [DEFINITION]: [LOCUS]: Construct_1 [ACCESSION]: pVec1
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(1693..1712)
                     /note="T3"
     primer_bind     complement(1730..1750)
                     /note="M13-rev"
     misc_binding    complement(1756..1778)
                     /note="LacO"
     promoter        complement(1783..1812)
                     /note="lac"
     rep_origin      complement(2118..2746)
                     /direction=LEFT
                     /note="ColE1 origin"
     CDS             complement(2898..3557)
                     /note="AmpR"
     promoter        complement(3797..3825)
                     /note="Amp prom"
ORIGIN
        1 agccaactgc tgttactctc gcccagaaat ctgcgccaac atggacacac ccagtggagc
       61 aagccatctg attttcatgg tcagattgta aagaagcaat tactcttgga cagtttaagc
      121 agaaaagcca ccaagtcaag tcactggtca tatgatgaac tcgaagatct aataaaagtt
      181 aatttaagcg ttcattacaa taaattcaag tgaatctgtt ttaagctagc ctttaacgtt
      241 tttaaattcc ttcaaacaaa ttggatcctc tttgcagttc ctcatttctc ggtgagctgg
      301 tgctttaatc cctttacttt ttcttcctct agggagtgat ggttggcatg ggacgaatta
      361 attcctgcag cccgggggta ccggtcgcca ccatgaacac cccgggaatt aacctgatca
      421 aggaggacat gcgcgtgaag gtgcacatgg agggcaacgt gaacggccac gccttcgtga
      481 tcgagggcga gggcaagggc aagccctacg agggcaccca gaccgccaac ctgaccgtga
      541 aggagggcgc ccccctgccc ttcagctacg acatcctgac caccgccgtg cactacggca
      601 accgggtgtt caccaagtac cccgaggaca tccccgacta cttcaagcag agcttccccg
      661 agggctacag ctgggagcgc accatgacct tcgaggacaa gggcatctgc accatccgca
      721 gcgacatcag cctggagggc gactgcttct tccagaacgt gcgcttcaag ggcaccaact
      781 tcccccccaa cggccccgtg atgcagaaga aaaccctgaa gtgggagccc agcaccgaga
      841 agctgcacgt gcgcgacggc ctgctggtgg gcaacatcaa catggccctg ctgctggagg
      901 gcggcggcca ctacctgtgc gacttcaaga ccacctacaa ggccaagaag gtggtgcagc
      961 tgcccgacgc ccacttcgtg gaccaccgca tcgagatcct gggcaacgac agcgactaca
     1021 acaaggtgaa gctgtacgag cacgccgtgg cccgctacag ccccctgccc agccaggtgt
     1081 ggtaatctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     1141 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     1201 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     1261 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg
     1321 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     1381 cattttttaa ccaataggcc gaaatcggcg gatcctgcgg ccgcaaggga aaatagaatg
     1441 aagtgatctc caaaaaataa gtactttttg actgtaaata aaattgtaag gagtaaaaag
     1501 tacttttttt tctaaaaaaa tgtaattaag taaaagtaaa agtattgatt tttaattgta
     1561 ctcaagtaaa gtaaaaatcc ccaaaaataa tacttaagta cagtaatcaa gtaaaattac
     1621 tcaagtactt tacacctctg gttcttgacc ccctaccttc agcaagccca gcagatccac
     1681 tagatctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
     1741 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     1801 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     1861 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     1921 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     1981 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2041 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     2101 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     2161 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2221 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2281 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     2341 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     2401 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     2461 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     2521 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     2581 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     2641 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     2701 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     2761 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     2821 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     2881 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     2941 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     3001 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     3061 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     3121 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     3181 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     3241 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     3301 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     3361 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     3421 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     3481 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     3541 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     3601 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     3661 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     3721 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     3781 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     3841 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta
     3901 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     3961 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     4021 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     4081 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     4141 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     4201 gacggggaaa gccggccaca atgacggcta ctacatggtg ccattccttc ctctttatag
     4261 gaatggagac tacctcctgt ccaacaaggc tcttggatac gagtacgcct acctgttgga
     4321 cccaggtcat tgcacaacac cagaaatgcc ctctgatctg caaaagacgt gaatatctgt
     4381 tcagacaccc atatccactc tgttccacac aggtcagagg tttgtccagg agttcttgac
     4441 agaggtgtaa aaagtactca aaaattttac tcaagtgaaa gtacaagtac ttagggaaaa
     4501 ttttactcaa ttaaaagtaa aagtatctgg ctagaatctt acttgagtaa aagtaaaaaa
     4561 gtactccatt aaaattgtac ttgagtatta aggaagtaaa aggctagcaa gatctgctcg
     4621 agagtttgac ttgggttgat tatagcaaat agtggaacac aaacagattt tgaaatattc
     4681 ttgcaaattt atttattaaa tccattcaaa tattgtcatt ttttgtactg tgtaatttat
     4741 attgatttgc tcatacaata tttacatttc gcttaaacaa tacactatta tttatattta
     4801 aaaatagcta tatataaatc aaatgtatag ctctttttta cacaattaat caaagctaaa
     4861 atatttgtca cacaaataca gtaagccact tcacaatgat atcttcaaaa tctgtgcttg
     4921 actgaatcat ttcatttatt gttttgtaat tcacattata ccttacgttc tccaggcaca
     4981 gtttgaatta ttgataaatg aagataatac atctgattta attagtctcc tttgtttata
     5041 ataactttaa tctaatttga tagtaataat tccagcaata catccattac aataaaaatc
     5101 gctttttaat tttgcacatt agtttgccgg tatttgttta aagtttcacc atacttttaa
     5161 atcatgtgct atttgaaaag ggctgttatg actggaatta aatgtttttt tttttatatc
     5221 agtgttttag attgaagtac aatatttata ggtaaaattc accagcatat gcttaaagat
     5281 agccatgctc caggtccttg tgtccttcta aggtctgttt gtggtttaag ctgtttgttg
     5341 acacacgctt gtcatctcca tttttctgtg cagttattgt cagtttattg cacactgggt
     5401 tgcattaagc tgcagttttt tcacaacagt tttccacatt gtaacagtca gtctccaaat
     5461 tattccatca gtcctcaaat ttagtcattt gaggccatga aaacacttaa aactacataa
     5521 aggtagtttg gcattaggtt ttagacttgt tcttgtttgg caatattgca acctgtgtaa
     5581 aataatttga ttcttttttt tttttttttt tttttaattt tcagaaagaa atggtccaaa
     5641 aggagctatg tgaacattta tccatgtgtt taatgttttt ttttttatta ttaaaccact
     5701 tctgacttaa ctttttttag ttattctatt ttgtgacgag tctgaatggc caaagtactt
     5761 ttgaatccaa aatcttacta gtaaaatgtt aaatcttact agcagtgttg ggtataattt
     5821 gtaacaaagt taaattccat atctgctaag tgaaaaagta aagtaagaga ttgctgtaaa
     5881 tatttaggta atttgtttta ttaatacaat atagaaagta acaaaacaca atgtaaaaaa
     5941 atcaataaag aaagtaaata ataaaagtaa tgataaacta atgatctaaa caaaattagt
     6001 aataaaattt atacaattca gggagggtaa agaatttcac ctaatagtca aactctgtat
     6061 taaataatat aaaatatttg ttcttagtct gaagaggaga ctgtgagaga aagcttactg
     6121 gttattaaat tagaaaattt gtaacttgca gattaaagct gtgtgccttg cagattaaaa
     6181 taatttaaat aatttaataa aggccatgtt tgagacaaat gtaaaaacat aataaatgtt
     6241 aagaatttca gatattgaga ttttacaatt taaaaaaagc aataaagtaa tagcagagac
     6301 ctacactctt aaagtaaatt actcaacact gcttactagt aacttttgga atagttacta
     6361 gtatccattg aacaaatact agaatctaat taaattgtgt gagtgtccaa tgaaaagatt
     6421 cttgtattta aaacagtacc agtatctata tatgtacaaa atttagtgct agcaaaatgc
     6481 aaataaatac tactgcatat aatggattgc attgtaaatc gacttgccat aatacattta
     6541 tttcctattt ttacttttaa caaaaaataa gttcactgag tatttcatgt cattaaatcg
     6601 cattaaacct cttgcatgct ttatgtacac tagtgcagtt cgctaaatga ccaccggtgt
     6661 cactaataac acgatcgttt ctgaattctg cattaaacgc catttatacg ttgctcttat
     6721 tagtaaacaa tttctccatc atgagctcga ccagtattgc atatttcatc ctcttaaatc
     6781 cagagttgtt tcttttaatc actttctctt catgataaaa gcagtttttg gttttcagaa
     6841 gtttacaaca aacccagtca gttattgcct cagcaaacac tggagcaact gcacacttgt
     6901 gaaaagcttc ttgcgacaca aacctttctc tggacttata aataaaggca ttcagcagct
     6961 ccaccataca cctccactgg tcttgataaa gcagatatca gcagtgatac aggtaccgcc
     7021 accatggatg aggaaatcgc tgccctggtc gttgacaacg gctccggtat gtgcaaagcc
     7081 ggttttgctg gagatgatgc ccctcgtgct gttttcccct ccattgttgg acgacccaga
     7141 catcaggtac atttaataaa ccatcctaat aacatgttaa tcatattgaa ccacattaaa
     7201 ctctcatttg tgcattttac atcttttaaa accgatttat attcgttttt agataatgaa
     7261 gtttattaaa gtgttaattc acagctttat tgcctgtttt gagaatctgc ttgcataagt
     7321 gatttttaaa cttgatctga ttctcgtttg tttcgaagtt cttcagcttt gaaagtcaga
     7381 tcttcattca cagccatgcc atagttttag gatacagtat aagtagacga attgggatgc
     7441 atcgtagatt ttatgctgtg tttgttatgg tttttcagtg gtagagattt tcagagtatt
     7501 attagggtta tagtcagtgt caggtttatt tatcgtcagc gtgatgggca aaaaacagct
     7561 ggcagtacgg gctcgcactt cacatctgta gccatttgaa gaatgaagag cagaaaaaac
     7621 tcatcccctc gagggtcttc ctcagtctgt catcctctcc tcagatgcag aacttagcca
     7681 ctgtgaacaa agagttcaat cactgttcac caatcagcta agctctgcac ctgctagaaa
     7741 agagttctcg gcctgcagag aaaagcctgc tcttcttcta ccttcatgat tttctggagc
     7801 tgcattaaga cggagcctct ggttggcata gcagccgtcc gggtggcggt tgtgtggtaa
     7861 tccctggcaa tgtgattttt atcttaagtc acaaatcagc acgcc
//
