LOCUS       Addgene-pAF256          8076 bp    DNA     circular UNA 01-FEB-2018
DEFINITION  pRPF185 - Ptet - HupA-Smbit - Lgbit.
ACCESSION   urn.local...b7-8m31heu
VERSION     urn.local...b7-8m31heu
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     rep_origin      complement(105..344)
     gene            complement(550..2187)
                     /standard_name="repA"
     CDS             complement(550..2187)
                     /standard_name="repA"
     gene            complement(2438..2974)
                     /standard_name="orfB"
     CDS             complement(2438..2974)
                     /standard_name="orfB"
     terminator      3284..3327
                     /standard_name="fdx terminator"
     misc_feature    3328..3331
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     gene            complement(3336..3959)
                     /standard_name="tetR"
     CDS             complement(3336..3959)
                     /standard_name="tetR"
     RBS             3965..3969
                     /standard_name="Shine Delgarno"
     misc_feature    3981..3999
                     /note="Geneious type: operator"
                     /standard_name="tetO"
     promoter        complement(3981..3986)
                     /standard_name="PtetR -10"
     promoter        complement(4004..4009)
                     /standard_name="PtetR -35"
     promoter        4061..4066
                     /standard_name="Pxyl -35"
     misc_feature    4066..4084
                     /note="Geneious type: operator"
                     /standard_name="tetO"
     promoter        4084..4089
                     /standard_name="Pxyl -10"
     RBS             4129..4150
                     /standard_name="RBS"
     gene            4151..4426
                     /standard_name="hupA*"
     CDS             4151..4516
                     /standard_name="HupA-SmBit"
     misc_feature    4436..4480
                     /standard_name="linker"
     misc_feature    4481..4513
                     /standard_name="SmBit"
     RBS             4521..4546
                     /standard_name="RBS"
     misc_feature    4547..5020
                     /standard_name="LgBit"
     CDS             4547..5023
                     /standard_name="LgBit"
     misc_feature    5028..5031
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     terminator      5060..5090
                     /standard_name="slpA terminator"
     misc_feature    complement(5370..5741)
                     /standard_name="traJ"
     oriT            5774..5843
                     /standard_name="oriT"
     gene            complement(6259..6882)
                     /standard_name="catP"
     CDS             complement(6259..6882)
                     /standard_name="catP"
     misc_feature    complement(7251..7804)
                     /standard_name="ColE1"
ORIGIN      
        1 gtgcaccaat tttttacttt tctattttag aatctagcat ttccaatgct tactaatact
       61 atttttgatt tttaacttgt tttaaaaaag cacctactaa ttaggattct agctctatat
      121 tctagttata cattctctat attctagctc tatattctag ttatacattc tctatattct
      181 agctctatat tctagttata cattctctat attctagctc tatattctag ttatacattc
      241 tctatattct agctctatat tctagttata cattctctat attctagctc tatattctag
      301 ttatacattc tctatattct agctctatat tctagttata cattctctaa aaaacatata
      361 tatatatact acatatatat gtacaattat tttaccaaaa gaaattttta aaacggctta
      421 atttcaatac tttgatgcca tttttttttt gcaattctcg tacaattatt ttaccaaaag
      481 aaatttttat taattagttt ttttgcataa aaataggact agaaaatcaa ttctaatcct
      541 atcaaaaatc tacttgttta atctatatgt atagtacata aatcctctat catttatttt
      601 agtttcccat acatctaaaa ttttagtatc taataaagat tctatcatat ttgaaattct
      661 ttgctgtgtt actcctaaat cttttgctaa ttcctcttga ttaattctta atatatttcc
      721 ttttatttta tcatctttga ccaataaatc ttgcttatat ctcatatgag tatataatct
      781 taattcttca gttgaaatta ttccccaaat tactgccatg gtaacaaagt aactaatagt
      841 atattgttta tctatattac atctaacttc ttttacattg tatgtatctt ttattcctag
      901 ctttgtaaca ccttttgttt ttgtaatata atcattatgt tgaagttctt ttaatgtttc
      961 tcttaatgtc ttttcactca taacaatgtt tttaactttc ttcttacgtt tataggttat
     1021 cagctccatt atatcgtcta tgtttaattc tctatcttta ttgttaagta acacattata
     1081 gataaacaat tgattaccag tcattatttt aacccctttc ctattcttat atttcaaatc
     1141 ctttgagtgc ttatgtggca tattgaacaa agtatcttca tcactatata taaaatctga
     1201 ttctatttta ttccaacaat cactattaaa ctcacaacct gtacaaccaa aattaaccgt
     1261 tttaagcttc tcatacatat acttgacttg atattctagc tcactatttg aaagactatt
     1321 ttgtgatata tagttccatt ctctagcaac ggatattact tgacttaaac ttttattcct
     1381 caatcttaaa gtcacaacta ttttttgcaa atctaggttt ttatgtccat ctttactacc
     1441 attttttaaa atatctacta tacaatttgg tagcttacta tctaatatat attttgtatt
     1501 agtattacct actgttttta catctttgca atatttttta gctaactttt ctatgtcata
     1561 tctatatata tttttgtctt ggtgtattat ttttacttgt ttagtagtat ttttaatatt
     1621 atatgtataa ggtactctta atacttgagt agataaattt gcttgtttat ctgcacccaa
     1681 tttatttatt aatgtatttg taacttctga tactaactta atgttattag ttttattaat
     1741 tagcacataa acatggaatc catttccact atcaactagg atatgataat gtaatcctat
     1801 cttagtaaat aaattaatta tatctttata attaaaacct tgtcctaatt cttttttgtc
     1861 aaaatcaaat cctaatgcat atctatttat taagttttca ttggttcctg cttttccatc
     1921 agttgtacat aggttaaaaa atgtattttc ataataaata ttttttgcga aggtaaattc
     1981 tactatatca tctatatttt taaaaaaagt tgttttacta tattctttaa attcttctcc
     2041 gagttctaat tgtttactat tacttttatt attttgaaat actctaacat attcattatc
     2101 aataagatta tttccttgtc ctataccatc tctaaatact gccattagaa attttttcaa
     2161 cttatatttt gaatctaatt gttccatata tttattatct ccttatctag tttttttaag
     2221 agataacact acattaaata tatacttatt gactaatcaa cttttacaac tattgaataa
     2281 ttttagctta gtatgtataa taatattgtt aagatgctaa aattgatagc aatattatta
     2341 tataactaca gatacttaat cagttagacc gatattggag ttggtagctc cataaactca
     2401 attggtagtt gagttgtcat tgtctagtag tattatctca ttttgttgtt atcttaaaat
     2461 attattttaa aaatactctg cttggtcggc ttagggtatt ttttttgttt aattccaata
     2521 ttggttcaaa ctcctgtgta atacttttga cacttccatc tgtattgaat tgacagttga
     2581 tatggtatct cattattggc ttgttcttat gtttttctat gtatttattt tttactttca
     2641 aataatttgt atttccattt ttagttatgt ttaacacact atccttatta ctcaataaaa
     2701 ttattttttg ttttaacata tgatttatag aatctaaaaa ttgatattga cttataatag
     2761 tactttctac ctctctagct ttttctagtg tttcaaattc ttcaaactct gctatattgc
     2821 tatagtatag cttatcatta ataatattat ctgacaactt taattccatt ttataaaaca
     2881 attccaagtt atgtataaaa tttttaatgt tacaacatat agacttatta tttgttctta
     2941 tatggtatct ccatttacta cttctagctc tcatgttata tgcacaacta ctcccaacat
     3001 aaacatatat tgcttgttct aactcatatt tttgtaaaat ttcaacatca tatccagtca
     3061 aatcgtactc tctcttatat acaaatggtt tttctaattt tttgttcaat aaatctcctc
     3121 ctatttctta cttaatttag ctaaaaatct atttatattt cacctccttt ttgactttaa
     3181 gcctacgaat accatattta tgattataac atcttctatt tttcccaaat ccttacatct
     3241 cccctaattt ttagacttaa gggcgaattc tgcatcaagc tagcataaaa ataagaagcc
     3301 tgcatttgca ggcttcttat ttttatggta ccttaagacc cactttcaca tttaagttgt
     3361 ttttctaatc cgcatatgat caattcaagg ccgaataaga aggctggctc tgcaccttgg
     3421 tgatcaaata attcgatagc ttgtcgtaat aatggcggca tactatcagt agtaggtgtt
     3481 tccctttctt ctttagcgac ttgatgctct tgatcttcca atacgcaacc taaagtaaaa
     3541 tgccccacag cgctgagtgc atataatgca ttctctagtg aaaaaccttg ttggcataaa
     3601 aaggctaatt gattttcgag agtttcatac tgtttttctg taggccgtgt acctaaatgt
     3661 acttttgctc catcgcgatg acttagtaaa gcacatctaa aacttttagc gttattacgt
     3721 aaaaaatctt gccagctttc cccttctaaa gggcaaaagt gagtatggtg cctatctaac
     3781 atctcaatgg ctaaggcgtc gagcaaagcc cgcttatttt ttacatgcca atacaatgta
     3841 ggctgctcta cacctagctt ctgggcgagt ttacgggttg ttaaaccttc gattccgacc
     3901 tcattaagca gctctaatgc gctgttaatc actttacttt tatctaatct agacatcatt
     3961 aattcctcct ttttgttgac attatatcat tgatagagtt atttgtcaaa ctagtttttt
     4021 atttcgatgc cctggacttc atgaaaaact aaaaaaaata ttgacactct atcattgata
     4081 gagtataatt aaaataagct tgatcgtagc gttaacagat ctgagctcct gcagtaaagg
     4141 agaaaatttt gtgaataaag ctgaattagt atcaaagatg gcagaaaaaa gtggattaac
     4201 aaagaaggaa gcagaagctg cgttaaacgc atttatgagt tctgttcaag atgcactagt
     4261 aaataatgaa aaagttcaat tagttggatt tggaacattt gagacaagag aaagagctgc
     4321 tagacaagga agaaatccaa gagatccaga gcaagttata gatataccag cttctaaagc
     4381 accagttttc aaagctggaa aaggattaaa ggatataata aatggatctc gagggggttc
     4441 tagtggtggt ggtggttctg gtggtggtgg ttctagtggt gttacaggtt atagactttt
     4501 tgaagaaatt ctatagctcg atcgctgcag taaaggagaa aattttgtgt ttacacttga
     4561 agattttgtg ggtgattggg aacaaactgc tgcatataat ttagatcaag ttttagaaca
     4621 gggtggagtg agttctcttt tacaaaatct tgcagtctca gttacaccaa tacaaagaat
     4681 agttagaagt ggagaaaatg cattaaagat cgatatacat gtaataattc cttatgaggg
     4741 actatcagca gaccaaatgg cacaaattga ggaagtattc aaagttgtat atccagtaga
     4801 cgatcatcac tttaaagtaa tattacctta tggaacttta gtaattgatg gtgtaacacc
     4861 aaatatgtta aattattttg gtagaccata cgaagggatt gcagtttttg atggaaagaa
     4921 aataaccgta acaggcactt tgtggaatgg aaataaaata atagatgaaa ggttaattac
     4981 acctgatgga tctatgttgt ttagagtaac tataaacagt taggctggat cctataagtt
     5041 ttaataaaac tttaaataga aaaaggcttc tctcatgaga agtctttttt atttaaaata
     5101 aatataaaat aaaatagagg ctataaatag cctctatttt atgtgagaaa tccctaaata
     5161 aaaagatgcc agtgtgctgg aattcgccct taggctgcgc tagggacctc tttagctcct
     5221 tggaagctgt cagtagtagg ggatcggtct tgccttgctc gtcggtgatg tacttcacca
     5281 gctccgcgaa gtcgctcttc ttgatggagc gcatggggac gtgcttggca atcacgcgca
     5341 ccccccggcc gttttagcgg ctaaaaaagt catggctctg ccctcgggcg gaccacgccc
     5401 atcatgacct tgccaagctc gtcctgcttc tcttcgatct tcgccagcag ggcgaggatc
     5461 gtggcatcac cgaaccgcgc cgtgcgcggg tcgtcggtga gccagagttt cagcaggccg
     5521 cccaggcggc ccaggtcgcc attgatgcgg gccagctcgc ggacgtgctc atagtccacg
     5581 acgcccgtga ttttgtagcc ctggccgacg gccagcaggt aggccgacag gctcatgccg
     5641 gccgccgccg ccttttcctc aatcgctctt cgttcgtctg gaaggcagta caccttgata
     5701 ggtgggctgc ccttcctggt tggcttggtt tcatcagcca tccgcttgcc ctcatctgtt
     5761 acgccggcgg tagccggcca gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc
     5821 gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg ggcctacttc acctatcctg
     5881 cccggctgac gccgttggat acaccaagga aagtctacac gaaccctttg gcaaaatcct
     5941 gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg ctataatgac cccgaagcag
     6001 gaagggcgaa ttctgcagat ctgaccggtc tctgaaaata taaaaaccac agattgatac
     6061 taaaaccttg gttgtgttgc ttttcggggc ttaaatcaag gaaaaatcct tgttttaagc
     6121 ctttcaaaaa gaaacacaag gtctttgtac taacctgtgg ttatgtataa aattgtagat
     6181 tttagggtaa caaaaaacac cgtatttcta cgatgttttt gcttaaatac ttgtttttag
     6241 ttacagacaa acctgaagtt aactatttat caattcctgc aattcgttta caaaacggca
     6301 aatgtgaaat ccgtcacata ctgcgtgatg aacttgaatt gccaaaggaa gtataatttt
     6361 gttatcttct ttataatatt tccccatagt aaaaatagga atcaaataat catatccttt
     6421 ctgcaaattc agattaaagc catcgaaggt tgaccacggt atcatagata cattaaaaat
     6481 gttttccgga gcatttggct ttccttccat tctatgattg tttccatacc gttgcgtatc
     6541 actttcataa tctgctaaaa atgatttaaa gtcagactta cactcagtcc aaaggctgga
     6601 aaatgtttca gtatcattgt gaaatattgt atagcttggt atcatctcat catatatccc
     6661 caattcacca tcttgattga ttgccgtcct aaactctgaa tggcggttta caatcattgc
     6721 aatataataa agcattgcag gatatagttt cattcccttt tcctttattt gtgtgatatc
     6781 cactttaacg gtcatgctgt aggtacaagg tacacttgca aagtagtggt caaaatactc
     6841 ttttctgttc caactatttt tatcaatttt ttcaaatacc atctaagttc cctctcaaat
     6901 tcaagtttat cgctctaatg aacaaagata ttataccaca tttttgtgaa tttttcaact
     6961 tgcccacttc gactgcactc ccgacttaat aacttcttga acacttgccg aaaaagaaaa
     7021 actgccgggt acgtacccgg ggatcgatcc ccggccgagc gctcttccgc ttcctcgctc
     7081 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7141 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7201 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7261 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7321 ctataaagat accaggcgtt tcccccttga agctccctcg tgcgctctcc tgttccgacc
     7381 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7441 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7501 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7561 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7621 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7681 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7741 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7801 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7861 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgaa atgcaagttt
     7921 ctaactaaca tacatcatag ttactaaact atggtaacta taattttatt aactatatta
     7981 agcactgatt agtactataa ctcaatataa gcatatcccc tgtatcgtaa ctagagaacc
     8041 aaacgacgga aaaagcgata tagataagtt tagata
//
