LOCUS       Exported                3898 bp ds-DNA     circular SYN 08-DEC-2017
DEFINITION  Ligation of NheI-NLS(NP)-mEGFP-XhoI into NheI/XhoI cut pGEMHE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 12, 2018 from SnapGene Viewer 4.1.2
            http://www.snapgene.com
COMMENT     Vector molecule:  NheI/XhoI cut pGEMHE
               Fragment ends: XhoI and NheI
               Fragment size: 3097
            Insert molecule:  NheI-NLS(NP)-mEGFP-XhoI
               Fragment ends: NheI and XhoI
               Fragment size: 801
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    664..680
                     /label=T7 promoter
     misc_feature    780..1643
                     /label=NLS(NP)-mEGFP
     misc_feature    849..1565
                     /label=mEGFP
                     /note="monomeric EGFP"
     misc_feature    complement(3102..3898)
                     /label=AmpR
ORIGIN
        1 aaacaggaag gcaaatgccg aaaaaaggga ataagggcga cacggaaatg ttgaatactc
       61 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
      121 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
      181 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
      241 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
      301 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
      361 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
      421 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
      481 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
      541 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      601 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
      661 ttgtaatacg actcactata gggcgaatta attcgagctc ggtacccagc ttgcttgttc
      721 tttttgcaga agctcagaat aaacgctcaa ctttggcaga tcaattcccc ggtgctagca
      781 tgaagcggcc cgctgctact aagaaggctg gtcaggctaa gaagaagaag cttccaccgg
      841 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      901 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      961 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     1021 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     1081 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1141 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     1201 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     1261 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     1321 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     1381 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     1441 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     1501 acatggtcct taaggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     1561 acaagtactc agatctcgag ctcaagcttc gaattctgca gtcgacggcc cgggatcctg
     1621 tacaagtaaa gcggccgctc tagataaatg atagcttgat ctggttacca ctaaaccagc
     1681 ctcaagaaca cccgaatgga gtctctaagc tacataatac caacttacac tttacaaaat
     1741 gttgtccccc aaaatgtagc cattcgtatc tgctcctaat aaaaagaaag tttcttcaca
     1801 ttctaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaacc cccccccccc ccccctgcag
     1861 gcgcgccgcc ggcgatttaa attaattaat ctagcttgag tattctatag tgtcacctaa
     1921 atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     1981 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     2041 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     2101 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     2161 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     2221 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2281 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2341 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2401 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2461 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2521 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2581 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2641 actatcgtct tgagtccaac ccggtaagac acgaccttat cgccactggc agcagccact
     2701 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     2761 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     2821 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     2881 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     2941 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     3001 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     3061 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     3121 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     3181 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     3241 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc ccggaagggc
     3301 cgagcgcaga agtggtcctg caaactttat ccgcctccat ccagtctatt aattgttgcc
     3361 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     3421 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     3481 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     3541 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     3601 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     3661 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     3721 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     3781 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     3841 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagc
//
