LOCUS       pAAV.CMV.PI.EGFP        5504 bp    DNA     circular     20-OCT-2017
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|652270879|
COMMENT     VNTDBDATE|-11991895|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV.CMV.PI.EGFP.WPRE.bGH (p0101)|
COMMENT     VNTAUTHORNAME|Shu-Jen Chen|
COMMENT     VNTOAUTHORNAME|Michael Yang|
COMMENT     VNTOAUTHORTEL|8-7117|
COMMENT     VNTOAUTHOREML|miyang@mail.med.upenn.edu|
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COMMENT                          "Foreign object. Author: Shu-Jen Chen. Original author: Michael Yang" 
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FEATURES             Location/Qualifiers
     repeat_region   1..130
                     /vntifkey="34"
                     /label=5'-ITR
     repeat_region   complement(2564..2693)
                     /vntifkey="34"
                     /label=3'-ITR
     promoter        211..793
                     /vntifkey="29"
                     /label=CMV\promotor
     intron          831..963
                     /vntifkey="15"
                     /label=PI
     CDS             981..1697
                     /vntifkey="4"
                     /label=eGFP
     misc_feature    1714..2255
                     /vntifkey="21"
                     /label=WPRE
     polyA_signal    2262..2476
                     /vntifkey="25"
                     /label=BGH\pA
     CDS             3457..4317
                     /vntifkey="4"
                     /label=Amp-R
BASE COUNT     1261 a      1476 c      1421 g      1346 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 
      181 aggaagatcg gaattcgccc ttaagctagc tagttattaa tagtaatcaa ttacggggtc 
      241 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc 
      301 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt 
      361 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca 
      421 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg 
      481 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca 
      541 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa 
      601 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa 
      661 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc 
      721 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg 
      781 tttagtgaac cgtcagatcc tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca 
      841 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac 
      901 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca 
      961 caggtgtcca ggcggccgcc atggtgagca agggcgagga gctgttcacc ggggtggtgc 
     1021 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg 
     1081 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 
     1141 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc 
     1201 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg 
     1261 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 
     1321 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 
     1381 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca 
     1441 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg 
     1501 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 
     1561 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg 
     1621 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 
     1681 tggacgagct gtacaagtaa taagcttgga tccaatcaac ctctggatta caaaatttgt 
     1741 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 
     1801 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 
     1861 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 
     1921 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 
     1981 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 
     2041 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 
     2101 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc 
     2161 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 
     2221 ctgctgccgg ctctgcggcc tcttccgcgt cttcgagatc tgcctcgact gtgccttcta 
     2281 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca 
     2341 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc 
     2401 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata 
     2461 gcaggcatgc tggggactcg agttaagggc gaattcccga ttaggatctt cctagagcat 
     2521 ggctacgtag ataagtagca tggcgggtta atcattaact acaaggaacc cctagtgatg 
     2581 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc 
     2641 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagccttaat 
     2701 taacctaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc 
     2761 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc 
     2821 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt 
     2881 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 
     2941 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 
     3001 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 
     3061 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccccga 
     3121 tagacggttt ttcgcccttt gacgctggag ttcacgttcc tcaatagtgg actcttgttc 
     3181 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttt 
     3241 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt 
     3301 aacaaaatat taacgtttat aatttcaggt ggcatctttc ggggaaatgt gcgcggaacc 
     3361 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 
     3421 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc 
     3481 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg 
     3541 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat 
     3601 ctcaatagtg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc 
     3661 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa 
     3721 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa 
     3781 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt 
     3841 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct 
     3901 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 
     3961 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagtaatggt aacaacgttg 
     4021 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 
     4081 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 
     4141 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 
     4201 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 
     4261 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 
     4321 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 
     4381 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 
     4441 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 
     4501 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 
     4561 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 
     4621 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 
     4681 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 
     4741 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 
     4801 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 
     4861 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 
     4921 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 
     4981 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 
     5041 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 
     5101 cggttcctgg ccttttgctg cggttttgct cacatgttct ttcctgcgtt atcccctgat 
     5161 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 
     5221 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct 
     5281 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa 
     5341 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct 
     5401 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac 
     5461 acaggaaaca gctatgacca tgattacgcc agatttaatt aagg 
//
