LOCUS       pAAV.CMV.LacZ.bG        7142 bp    DNA     circular     20-OCT-2017
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11129263|
COMMENT     VNTDBDATE|-11991921|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV.CMV.LacZ.bGH(p0102)|
COMMENT     VNTAUTHORNAME|Andres Fernandez|
COMMENT     VNTOAUTHORNAME|System Manager|
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COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 
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COMMENT        #295=(CSampFrag "pAAV.CMV.LacZ1 (p0102) Q" 2514 4437 "PvuII" 6951 
COMMENT              "PvuII" (LOGPEN 0 0 0) 2.94533e-316 5.29372e+180) 
COMMENT        #296=(CSampFrag "pAAV.CMV.LacZ1 (p0102) Q" 1536 1947 "EcoRV" 3483 
COMMENT              "PvuII" (LOGPEN 0 0 0) 2.99183e-316 2.57671e+097) 
COMMENT        #297=(CSampFrag "pAAV.CMV.LacZ1 (p0102) Q" 1117 6951 "PvuII" 926 "PvuII" 
COMMENT              (LOGPEN 0 0 0) 3.18586e-317 1.02895e-314) 
COMMENT        #298=(CSampFrag "pAAV.CMV.LacZ1 (p0102) Q" 1021 926 "PvuII" 1947 "EcoRV" 
COMMENT              (LOGPEN 0 0 0) 2.94533e-316 2.5779e-312) 
COMMENT        #299=(CSampFrag "pAAV.CMV.LacZ1 (p0102) Q" 591 3846 "PvuII" 4437 "PvuII" 
COMMENT              (LOGPEN 0 0 0) 2.94533e-316 2.5779e-312) 
COMMENT        #300=(CSampFrag "pAAV.CMV.LacZ1 (p0102) Q" 363 3483 "PvuII" 3846 "PvuII" 
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FEATURES             Location/Qualifiers
     LTR             1..130
                     /vntifkey="19"
                     /label=5'-ITR
     LTR             complement(4202..4331)
                     /vntifkey="19"
                     /label=3'-ITR
     misc_feature    4156..4201
                     /vntifkey="21"
                     /label=additional\AAV\sequences
     promoter        211..793
                     /vntifkey="29"
                     /label=CMV\promotor
     CDS             819..3893
                     /vntifkey="4"
                     /label=lacZ
     polyA_signal    3900..4120
                     /vntifkey="25"
                     /label=BGH\pA
     misc_feature    6126..6714
                     /vntifkey="21"
                     /label=Origin
     CDS             5095..5952
                     /vntifkey="4"
                     /label=Amp-R
BASE COUNT     1649 a      1875 c      1924 g      1694 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 
      181 aggaagatcg gaattcgccc ttaagctagc tagttattaa tagtaatcaa ttacggggtc 
      241 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc 
      301 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt 
      361 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca 
      421 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg 
      481 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca 
      541 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa 
      601 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa 
      661 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc 
      721 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg 
      781 tttagtgaac cgtcagatcc tgcagataag cggccgccat gaccatgatt acggattcac 
      841 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc 
      901 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc 
      961 cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag 
     1021 aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc 
     1081 cctcaaactg gcagatgcac ggttacgatg cgcccatcta caccaacgtg acctatccca 
     1141 ttacggtcaa tccgccgttt gttcccacgg agaatccgac gggttgttac tcgctcacat 
     1201 ttaatgttga tgaaagctgg ctacaggaag gccagacgcg aattattttt gatggcgtta 
     1261 actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt 
     1321 tgccgtctga atttgacctg agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga 
     1381 tggtgctgcg ctggagtgac ggcagttatc tggaagatca ggatatgtgg cggatgagcg 
     1441 gcattttccg tgacgtctcg ttgctgcata aaccgactac acaaatcagc gatttccatg 
     1501 ttgccactcg ctttaatgat gatttcagcc gcgctgtact ggaggctgaa gttcagatgt 
     1561 gcggcgagtt gcgtgactac ctacgggtaa cagtttcttt atggcagggt gaaacgcagg 
     1621 tcgccagcgg caccgcgcct ttcggcggtg aaattatcga tgagcgtggt ggttatgccg 
     1681 atcgcgtcac actacgtctg aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga 
     1741 atctctatcg tgcggtggtt gaactgcaca ccgccgacgg cacgctgatt gaagcagaag 
     1801 cctgcgatgt cggtttccgc gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca 
     1861 agccgttgct gattcgaggc gttaaccgtc acgagcatca tcctctgcat ggtcaggtca 
     1921 tggatgagca gacgatggtg caggatatcc tgctgatgaa gcagaacaac tttaacgccg 
     1981 tgcgctgttc gcattatccg aaccatccgc tgtggtacac gctgtgcgac cgctacggcc 
     2041 tgtatgtggt ggatgaagcc aatattgaaa cccacggcat ggtgccaatg aatcgtctga 
     2101 ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg 
     2161 atcgtaatca cccgagtgtg atcatctggt cgctggggaa tgaatcaggc cacggcgcta 
     2221 atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg 
     2281 aaggcggcgg agccgacacc acggccaccg atattatttg cccgatgtac gcgcgcgtgg 
     2341 atgaagacca gcccttcccg gctgtgccga aatggtccat caaaaaatgg ctttcgctac 
     2401 ctggagagac gcgcccgctg atcctttgcg aatacgccca cgcgatgggt aacagtcttg 
     2461 gcggtttcgc taaatactgg caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg 
     2521 tctgggactg ggtggatcag tcgctgatta aatatgatga aaacggcaac ccgtggtcgg 
     2581 cttacggcgg tgattttggc gatacgccga acgatcgcca gttctgtatg aacggtctgg 
     2641 tctttgccga ccgcacgccg catccagcgc tgacggaagc aaaacaccag cagcagtttt 
     2701 tccagttccg tttatccggg caaaccatcg aagtgaccag cgaatacctg ttccgtcata 
     2761 gcgataacga gctcctgcac tggatggtgg cgctggatgg taagccgctg gcaagcggtg 
     2821 aagtgcctct ggatgtcgct ccacaaggta aacagttgat tgaactgcct gaactaccgc 
     2881 agccggagag cgccgggcaa ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg 
     2941 catggtcaga agccgggcac atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca 
     3001 gtgtgacgct ccccgccgcg tcccacgcca tcccgcatct gaccaccagc gaaatggatt 
     3061 tttgcatcga gctgggtaat aagcgttggc aatttaaccg ccagtcaggc tttctttcac 
     3121 agatgtggat tggcgataaa aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg 
     3181 caccgctgga taacgacatt ggcgtaagtg aagcgacccg cattgaccct aacgcctggg 
     3241 tcgaacgctg gaaggcggcg ggccattacc aggccgaagc agcgttgttg cagtgcacgg 
     3301 cagatacact tgctgatgcg gtgctgatta cgaccgctca cgcgtggcag catcagggga 
     3361 aaaccttatt tatcagccgg aaaacctacc ggattgatgg tagtggtcaa atggcgatta 
     3421 ccgttgatgt tgaagtggcg agcgatacac cgcatccggc gcggattggc ctgaactgcc 
     3481 agctggcgca ggtagcagag cgggtaaact ggctcggatt agggccgcaa gaaaactatc 
     3541 ccgaccgcct tactgccgcc tgttttgacc gctgggatct gccattgtca gacatgtata 
     3601 ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg 
     3661 gcccacacca gtggcgcggc gacttccagt tcaacatcag ccgctacagt caacagcaac 
     3721 tgatggaaac cagccatcgc catctgctgc acgcggaaga aggcacatgg ctgaatatcg 
     3781 acggtttcca tatggggatt ggtggcgacg actcctggag cccgtcagta tcggcggaat 
     3841 tccagctgag cgccggtcgc taccattacc agttggtctg gtgtcaaaaa taaggatctg 
     3901 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct 
     3961 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc 
     4021 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg 
     4081 aggattggga agacaatagc aggcatgctg gggactcgag ttaagggcga attcccgata 
     4141 aggatcttcc tagagcatgg ctacgtagat aagtagcatg gcgggttaat cattaactac 
     4201 aaggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag 
     4261 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag 
     4321 cgagcgcgca gccttaatta acctaattca ctggccgtcg ttttacaacg tcgtgactgg 
     4381 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg 
     4441 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc 
     4501 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc 
     4561 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt 
     4621 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc 
     4681 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt 
     4741 agtgggccat cgccccgata gacggttttt cgccctttga cgctggagtt cacgttcctc 
     4801 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt 
     4861 gatttataag ggatttttcc gatttcggcc tattggttaa aaaatgagct gatttaacaa 
     4921 aaatttaacg cgaattttaa caaaatatta acgtttataa tttcaggtgg catctttcgg 
     4981 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 
     5041 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 
     5101 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 
     5161 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 
     5221 ggttacatcg aactggatct caatagtggt aagatccttg agagttttcg ccccgaagaa 
     5281 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 
     5341 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 
     5401 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 
     5461 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 
     5521 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 
     5581 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 
     5641 gtaatggtaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 
     5701 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 
     5761 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 
     5821 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 
     5881 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 
     5941 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 
     6001 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 
     6061 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 
     6121 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 
     6181 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 
     6241 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 
     6301 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 
     6361 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 
     6421 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 
     6481 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 
     6541 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 
     6601 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 
     6661 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 
     6721 agcaacgcgg cctttttacg gttcctggcc ttttgctgcg gttttgctca catgttcttt 
     6781 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 
     6841 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 
     6901 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 
     6961 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 
     7021 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 
     7081 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccag atttaattaa 
     7141 gg 
//
