LOCUS       pAAV.CMV.ffLucif        5889 bp    DNA     circular     20-OCT-2017
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11992038|
COMMENT     VNTDBDATE|-11992038|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV.CMV.ffLuciferase.SV40(p0105)|
COMMENT     VNTAUTHORNAME|Demo User|
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FEATURES             Location/Qualifiers
     CDS             973..2625
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase luc2"
                     /protein_id="AAV52869.1"
                     /db_xref="GI:55535619"
                     /vntifkey="4"
                     /label=Luc2
     repeat_region   1..168
                     /vntifkey="34"
                     /label=ITR
     repeat_region   complement(2901..3068)
                     /vntifkey="34"
                     /label=ITR
     polyA_signal    2635..2873
                     /vntifkey="25"
                     /label=SV40\late\polyadenylation\signal
     CDS             3831..4688
                     /vntifkey="4"
                     /label=Amp-R
     misc_feature    4862..5450
                     /vntifkey="21"
                     /label=Origin
     promoter        170..911
                     /vntifkey="29"
                     /label=CMV
BASE COUNT     1449 a      1537 c      1499 g      1404 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 
      181 aggaagatct tcaatattgg ccattagcca tattattcat tggttatata gcataaatca 
      241 atattggcta ttggccattg catacgttgt atctatatca taatatgtac atttatattg 
      301 gctcatgtcc aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat 
      361 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg 
      421 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 
      481 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 
      541 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg 
      601 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact 
      661 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 
      721 ggcagtacac caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 
      781 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 
      841 gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 
      901 taagcagagc tcgtttagtg aaccgtcaga tcactagaag cttggcaatc cggtactgtt 
      961 ggtaaagcca ccatggaaga tgccaaaaac attaagaagg gcccagcgcc attctaccca 
     1021 ctcgaagacg ggaccgccgg cgagcagctg cacaaagcca tgaagcgcta cgccctggtg 
     1081 cccggcacca tcgcctttac cgacgcacat atcgaggtgg acattaccta cgccgagtac 
     1141 ttcgagatga gcgttcggct ggcagaagct atgaagcgct atgggctgaa tacaaaccat 
     1201 cggatcgtgg tgtgcagcga gaatagcttg cagttcttca tgcccgtgtt gggtgccctg 
     1261 ttcatcggtg tggctgtggc cccagctaac gacatctaca acgagcgcga gctgctgaac 
     1321 agcatgggca tcagccagcc caccgtcgta ttcgtgagca agaaagggct gcaaaagatc 
     1381 ctcaacgtgc aaaagaagct accgatcata caaaagatca tcatcatgga tagcaagacc 
     1441 gactaccagg gcttccaaag catgtacacc ttcgtgactt cccatttgcc acccggcttc 
     1501 aacgagtacg acttcgtgcc cgagagcttc gaccgggaca aaaccatcgc cctgatcatg 
     1561 aacagtagtg gcagtaccgg attgcccaag ggcgtagccc taccgcaccg caccgcttgt 
     1621 gtccgattca gtcatgcccg cgaccccatc ttcggcaacc agatcatccc cgacaccgct 
     1681 atcctcagcg tggtgccatt tcaccacggc ttcggcatgt tcaccacgct gggctacttg 
     1741 atctgcggct ttcgggtcgt gctcatgtac cgcttcgagg aggagctatt cttgcgcagc 
     1801 ttgcaagact ataagattca atctgccctg ctggtgccca cactatttag cttcttcgct 
     1861 aagagcactc tcatcgacaa gtacgaccta agcaacttgc acgagatcgc cagcggcggg 
     1921 gcgccgctca gcaaggaggt aggtgaggcc gtggccaaac gcttccacct accaggcatc 
     1981 cgccagggct acggcctgac agaaacaacc agcgccattc tgatcacccc cgaaggggac 
     2041 gacaagcctg gcgcagtagg caaggtggtg cccttcttcg aggctaaggt ggtggacttg 
     2101 gacaccggta agacactggg tgtgaaccag cgcggcgagc tgtgcgtccg tggccccatg 
     2161 atcatgagcg gctacgttaa caaccccgag gctacaaacg ctctcatcga caaggacggc 
     2221 tggctgcaca gcggcgacat cgcctactgg gacgaggacg agcacttctt catcgtggac 
     2281 cggctgaaga gcctgatcaa atacaagggc taccaggtag ccccagccga actggagagc 
     2341 atcctgctgc aacaccccaa catcttcgac gccggggtcg ccggcctgcc cgacgacgat 
     2401 gccggcgagc tgcccgccgc agtcgtcgtg ctggaacacg gtaaaaccat gaccgagaag 
     2461 gagatcgtgg actatgtggc cagccaggtt acaaccgcca agaagctgcg cggtggtgtt 
     2521 gtgttcgtgg acgaggtgcc taaaggactg accggcaagt tggacgcccg caagatccgc 
     2581 gagattctca ttaaggccaa gaagggcggc aagatcgccg tgtaataatt ctagggccgc 
     2641 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt 
     2701 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa 
     2761 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg 
     2821 agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg 
     2881 atcttcctag agcatggcta cgtagataag tagcatggcg ggttaatcat taactacaag 
     2941 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc 
     3001 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga 
     3061 gcgcgcagcc ttaattaacc taattcactg gccgtcgttt tacaacgtcg tgactgggaa 
     3121 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt 
     3181 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa 
     3241 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 
     3301 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 
     3361 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga 
     3421 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 
     3481 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 
     3541 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 
     3601 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 
     3661 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa 
     3721 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 
     3781 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc 
     3841 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 
     3901 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt 
     3961 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt 
     4021 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg 
     4081 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact 
     4141 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg 
     4201 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 
     4261 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg 
     4321 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa 
     4381 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac 
     4441 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 
     4501 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca 
     4561 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 
     4621 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 
     4681 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 
     4741 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 
     4801 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 
     4861 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 
     4921 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 
     4981 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact 
     5041 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 
     5101 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 
     5161 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 
     5221 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 
     5281 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 
     5341 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 
     5401 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 
     5461 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 
     5521 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc 
     5581 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa 
     5641 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt 
     5701 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt 
     5761 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg 
     5821 gataacaatt tcacacagga aacagctatg accatgatta cgccagattt aattaaggcc 
     5881 ttaattagg 
//
