LOCUS       pAAV.TBG.PI.EGFP        5611 bp    DNA     circular     20-OCT-2017
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDATE|-11992198|
COMMENT     VNTDBDATE|-11992198|
COMMENT     LSOWNER|
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FEATURES             Location/Qualifiers
     CDS             3564..4421
                     /vntifkey="4"
                     /label=Amp-R
     misc_feature    4595..5183
                     /vntifkey="21"
                     /label=Origin
     misc_feature    2626..2671
                     /vntifkey="21"
                     /label=additional\AAV\sequences
     intron          939..1071
                     /vntifkey="15"
                     /label=SV40\misc\intron\(Promega)
     CDS             1090..1805
                     /vntifkey="4"
                     /label=eGFP
     misc_feature    1822..2363
                     /vntifkey="21"
                     /label=WPRE
     polyA_signal    2370..2584
                     /vntifkey="25"
                     /label=BGH\pA
     enhancer        211..310
                     /vntifkey="9"
                     /label=alpha\mic/bik
     enhancer        317..416
                     /vntifkey="9"
                     /label=alpha\mic/bik
     repeat_region   1..168
                     /vntifkey="34"
                     /label=5'\ITR
     repeat_region   complement(2634..2801)
                     /vntifkey="34"
                     /label=3'\ITR
     misc_feature    431..907
                     /vntifkey="21"
                     /label=TBG\promoter
BASE COUNT     1293 a      1502 c      1407 g      1409 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 
      181 aggaagatcg gaattcgccc ttaagctagc aggttaattt ttaaaaagca gtcaaaagtc 
      241 caagtggccc ttggcagcat ttactctctc tgtttgctct ggttaataat ctcaggagca 
      301 caaacattcc agatccaggt taatttttaa aaagcagtca aaagtccaag tggcccttgg 
      361 cagcatttac tctctctgtt tgctctggtt aataatctca ggagcacaaa cattccagat 
      421 ccggcgcgcc agggctggaa gctacctttg acatcatttc ctctgcgaat gcatgtataa 
      481 tttctacaga acctattaga aaggatcacc cagcctctgc ttttgtacaa ctttccctta 
      541 aaaaactgcc aattccactg ctgtttggcc caatagtgag aactttttcc tgctgcctct 
      601 tggtgctttt gcctatggcc cctattctgc ctgctgaaga cactcttgcc agcatggact 
      661 taaacccctc cagctctgac aatcctcttt ctcttttgtt ttacatgaag ggtctggcag 
      721 ccaaagcaat cactcaaagt tcaaacctta tcattttttg ctttgttcct cttggccttg 
      781 gttttgtaca tcagctttga aaataccatc ccagggttaa tgctggggtt aatttataac 
      841 taagagtgct ctagttttgc aatacaggac atgctataaa aatggaaaga tgttgctttc 
      901 tgagagactg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag gttacaagac 
      961 aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc ttgcgtttct 
     1021 gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca ggtgtccagg 
     1081 cggccgccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg 
     1141 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg 
     1201 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct 
     1261 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc 
     1321 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca 
     1381 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg 
     1441 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc 
     1501 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc 
     1561 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc 
     1621 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg 
     1681 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc 
     1741 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt 
     1801 acaagtaata agcttggatc caatcaacct ctggattaca aaatttgtga aagattgact 
     1861 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 
     1921 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg 
     1981 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg 
     2041 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg 
     2101 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc 
     2161 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca 
     2221 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc 
     2281 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct 
     2341 ctgcggcctc ttccgcgtct tcgagatctg cctcgactgt gccttctagt tgccagccat 
     2401 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc 
     2461 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg 
     2521 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg 
     2581 gggactcgag ttaagggcga attcccgata aggatcttcc tagagcatgg ctacgtagat 
     2641 aagtagcatg gcgggttaat cattaactac aaggaacccc tagtgatgga gttggccact 
     2701 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg 
     2761 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gccttaatta acctaattca 
     2821 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 
     2881 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 
     2941 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta 
     3001 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 
     3061 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 
     3121 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 
     3181 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 
     3241 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 
     3301 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 
     3361 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta 
     3421 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 
     3481 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 
     3541 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct 
     3601 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag 
     3661 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta 
     3721 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc 
     3781 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca 
     3841 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg 
     3901 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg 
     3961 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca 
     4021 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa 
     4081 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa 
     4141 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 
     4201 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 
     4261 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc 
     4321 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 
     4381 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 
     4441 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 
     4501 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 
     4561 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 
     4621 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 
     4681 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 
     4741 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 
     4801 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 
     4861 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 
     4921 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 
     4981 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 
     5041 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 
     5101 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 
     5161 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 
     5221 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 
     5281 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 
     5341 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt 
     5401 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc 
     5461 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc 
     5521 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct 
     5581 atgaccatga ttacgccaga tttaattaag g 
//
