LOCUS       pAAV.TBG\PI.Null        4568 bp    DNA     circular     21-OCT-2017
DEFINITION  Construction option #4 of p.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11992880|
COMMENT     VNTDBDATE|-11992880|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV.TBG PI.Null.bGH(p0148)|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Xiangyang Zhou|
COMMENT     VNTOAUTHORTEL|215-898-7117|
COMMENT     VNTOAUTHOREML|zhou3@mail.med.upenn.edu|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "Construction option #4 of p." 1) 
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COMMENT                          "Currently local object. Original author: Xiangyang Zhou" 
COMMENT                          1) 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "additional AAV sequences" "@N" 1 15.5197 
COMMENT                                   -6.96932 0 -10 10.1904 1.07399 #124#)) 
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COMMENT                      #126=(CGroupWidget 
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COMMENT                     (CObjectList) (CObjectList)) 
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COMMENT                     (CObjectList) (CObjectList)) 
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FEATURES             Location/Qualifiers
     repeat_region   1..130
                     /vntifkey="34"
                     /label=5'-ITR
     repeat_region   complement(1628..1757)
                     /vntifkey="34"
                     /label=3'-ITR
     misc_feature    1582..1627
                     /vntifkey="21"
                     /label=additional\AAV\sequences
     intron          939..1071
                     /vntifkey="15"
                     /label=SV40\misc\intron\(Promega)
     polyA_signal    1326..1540
                     /vntifkey="25"
                     /label=BGH-Poly\A
     promoter        205..913
                     /vntifkey="29"
                     /label=TBG
     TATA_signal     875..878
                     /vntifkey="41"
                     /label=TATA
     CDS             2521..3381
                     /vntifkey="4"
                     /label=Amp-R
BASE COUNT     1096 a      1148 c      1133 g      1191 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 
      181 aggaagatcg gaattcgccc ttaagctagc aggttaattt ttaaaaagca gtcaaaagtc 
      241 caagtggccc ttggcagcat ttactctctc tgtttgctct ggttaataat ctcaggagca 
      301 caaacattcc agatccaggt taatttttaa aaagcagtca aaagtccaag tggcccttgg 
      361 cagcatttac tctctctgtt tgctctggtt aataatctca ggagcacaaa cattccagat 
      421 ccggcgcgcc agggctggaa gctacctttg acatcatttc ctctgcgaat gcatgtataa 
      481 tttctacaga acctattaga aaggatcacc cagcctctgc ttttgtacaa ctttccctta 
      541 aaaaactgcc aattccactg ctgtttggcc caatagtgag aactttttcc tgctgcctct 
      601 tggtgctttt gcctatggcc cctattctgc ctgctgaaga cactcttgcc agcatggact 
      661 taaacccctc cagctctgac aatcctcttt ctcttttgtt ttacatgaag ggtctggcag 
      721 ccaaagcaat cactcaaagt tcaaacctta tcattttttg ctttgttcct cttggccttg 
      781 gttttgtaca tcagctttga aaataccatc ccagggttaa tgctggggtt aatttataac 
      841 taagagtgct ctagttttgc aatacaggac atgctataaa aatggaaaga tgttgctttc 
      901 tgagagactg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag gttacaagac 
      961 aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc ttgcgtttct 
     1021 gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca ggtgtccagg 
     1081 cggccaattc gaatggccat gggacgtcga cctgaggtaa ttataacccg ggccctatat 
     1141 atggatccaa ttgcaatgat catcatgaca gatctgcgcg cgatcgattg gccacgtggg 
     1201 ccgtgcacct taagcttatg gggattggtg gcgacgactc ctggagcccg tcagtatcgg 
     1261 cggaattcca gctgagcgcc ggtcgctacc attaccagtt ggtctggtgt caaaaataag 
     1321 gatctgcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc 
     1381 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg 
     1441 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca 
     1501 agggggagga ttgggaagac aatagcaggc atgctgggga ctcgagttaa gggcgaattc 
     1561 ccgattagga tcttcctaga gcatggctac gtagataagt agcatggcgg gttaatcatt 
     1621 aactacaagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc 
     1681 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg 
     1741 agcgagcgag cgcgcagcct taattaacct aattcactgg ccgtcgtttt acaacgtcgt 
     1801 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc 
     1861 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg 
     1921 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 
     1981 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 
     2041 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta 
     2101 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 
     2161 tcacgtagtg ggccatcgcc ccgatagacg gtttttcgcc ctttgacgct ggagttcacg 
     2221 ttcctcaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat 
     2281 tcttttgatt tataagggat ttttccgatt tcggcctatt ggttaaaaaa tgagctgatt 
     2341 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttataatttc aggtggcatc 
     2401 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 
     2461 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 
     2521 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct 
     2581 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca 
     2641 cgagtgggtt acatcgaact ggatctcaat agtggtaaga tccttgagag ttttcgcccc 
     2701 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc 
     2761 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg 
     2821 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta 
     2881 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc 
     2941 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt 
     3001 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg 
     3061 cctgtagtaa tggtaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct 
     3121 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc 
     3181 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct 
     3241 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac 
     3301 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc 
     3361 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat 
     3421 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 
     3481 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 
     3541 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 
     3601 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 
     3661 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta 
     3721 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 
     3781 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 
     3841 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 
     3901 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 
     3961 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 
     4021 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 
     4081 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 
     4141 aacgccagca acgcggcctt tttacggttc ctggcctttt gctgcggttt tgctcacatg 
     4201 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct 
     4261 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa 
     4321 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg 
     4381 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag 
     4441 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga 
     4501 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccagattt 
     4561 aattaagg 
//
