LOCUS       pENN\AAV.CMV.PI.        5342 bp    DNA     circular     21-OCT-2017
SOURCE      
  ORGANISM  
COMMENT       #1: FRAGMENT of <a href="vnti:dnarna/pENN%20AAV%20CMV%20PI%28p1059%29?sel=1043-1036">pENN AAV CMV PI(p1059)</a> 
                parent position: from 1043 to 1036 (+ fill of 4)
                original length: 4265
                molecule position: from 2109 to 1034
                Left Terminus
                    MluI site #1
                Right Terminus
                    EcoRI site #1
                      Completely filled in
              #2: FRAGMENT of <a href="vnti:dnarna/pBS185%20%28Putative%29?sel=771-1844">pBS185 (Putative)</a> 
                parent position: from 771 to 1844
                original length: 1074
                molecule position: from 1035 to 2108
                Left Terminus
                    BsrGI site #2
                      Completely filled in
                Right Terminus
                    MluI site #1
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11992885|
COMMENT     VNTDBDATE|-11992885|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pENN AAV.CMV.PI.Cre.rBG(p1090-R)|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Xiangyang Zhou|
COMMENT     VNTOAUTHORTEL|215-898-7117|
COMMENT     VNTOAUTHOREML|zhou3@mail.med.upenn.edu|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pENN AAV.CMV.PI.Cre.rBG(p1090-R)" 0 5342 
COMMENT       (CDBMol 0 0 1 3232 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 40 100 0 1 0 
COMMENT        (CHomObj 1 0 0 3 85) (CWordArray) (CWordArray) 
COMMENT        (CStringList "XmaI" "NspI") (CStringList "atg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList #0=(COligo "12F1" "CTTTAATATGTTGTTGTTTTG" "" 0 (CStringList) 0) 
COMMENT         #1=(COligo "AAV2Cap-1F" "GGGAAAGGTGCCAGACGCTT" "" 0 (CStringList) 0) 
COMMENT         #2=(COligo "AAV2Cap-2F" "GCCTCTCGGACAGCCACCAG" "" 0 (CStringList) 0) 
COMMENT         #3=(COligo "AAV2Cap-3F" "GCTGCGTACCGGAAACAACTTTACC" "" 0 (CStringList) 
COMMENT             0) 
COMMENT         #4=(COligo "AAV2Cap-4F" "GCCCATCTGGGCAAAGATTCC" "" 0 (CStringList) 0) 
COMMENT         #5=(COligo "AAV2Rep-1F" "AGCGCCTGTTTGAATCTCACGG" "" 0 (CStringList) 0) 
COMMENT         #6=(COligo "AAV2Rep-2F" "AAGCAAGGTGCGCGTGGACCAG" "" 0 (CStringList) 0) 
COMMENT         #7=(COligo "AAV2Rep-R" "CGCGAATCTGACTCAGGAAACGTCC" "" 0 (CStringList) 0) 
COMMENT         #8=(COligo "AAV7Cap-F" "CTGGGCCAAGATTCCTCACACGG" "" 0 (CStringList) 0) 
COMMENT         #9=(COligo "AAV8 BK-R" "TCAGGGTTATTGTCTCATGAGCGG" "" 0 (CStringList) 0) 
COMMENT         #10=(COligo "AAV8 Cap-1F" "GACGGCAACTTCCACCCGTCTC" "" 0 (CStringList) 0) 
COMMENT         #11=(COligo "AAV8 Cap-2F" "ACCTCTCGGTCTGGTTGAGGAAGGC" "" 0 
COMMENT              (CStringList) 0) 
COMMENT         #12=(COligo "AAV8 Cap-3F" "ACGCAGAATGAAGGCACCAAGAC" "" 0 (CStringList) 
COMMENT              0) 
COMMENT         #13=(COligo "AAV8 Cap-4F" "AGACTCTGGGCTTCAGCCAAGG" "" 0 (CStringList) 0) 
COMMENT         #14=(COligo "AAV8 Rep-1F" "TCCGCGCCCGACTACCTGGTG" "" 0 (CStringList) 0) 
COMMENT         #15=(COligo "AAV8 Rep-1R" "CCGCGGTAGATGGTCTGGACC" "For AAV8 Rep" 0 
COMMENT              (CStringList) 0) 
COMMENT         #16=(COligo "AAV8 Rep-2F" "AAGCGAGCCCAAGCGGGCCT" "" 0 (CStringList) 0) 
COMMENT         #17=(COligo "AAV8 Rep-F" "CGAGAAGGGCGAGAGCTACTTTC" "" 0 (CStringList) 0) 
COMMENT         #18=(COligo "Ad CMV-F" "AACAACTCCGCCCCATTGACGC" "" 0 (CStringList) 0) 
COMMENT         #19=(COligo "Adeasy1-F" "GCGTAACCGAGTAAGATTTGGC" "" 0 (CStringList) 0) 
COMMENT         #20=(COligo "Adeasy2-R" "ATACAAAACTACATAAGACCCCCACC" "" 0 (CStringList) 
COMMENT              0) 
COMMENT         #21=(COligo "Ap2 F1" "TATGGCCAAGGGAGTGCTCG" "For pSK + Ap2 plasmid" 0 
COMMENT              (CStringList) 0) 
COMMENT         #22=(COligo "Ap2 F2" "TCACAGGGTCAGTTGAAAGAAGGC" "for pSK + Ap2 plasmid" 
COMMENT              0 (CStringList) 0) 
COMMENT         #23=(COligo "Ap2 R1" "AACAGCAGCAGTATGCGTGGC" "For pSK + Ap2 plasmid" 0 
COMMENT              (CStringList) 0) 
COMMENT         #24=(COligo "BGH-R" "CAACAGATGGCTGGCAACTAGAAGG" 
COMMENT              "REV primer against the 5' terminus of the BGH polyA" 0 
COMMENT              (CStringList "ANTISENSE_PRIMER" "PCR_PRIMER" "SEQUENCING_PRIMER") 
COMMENT              0) 
COMMENT         #25=(COligo "Bgl2-H1F" "GATCAGATCTATTTGCATGTCGCTATGTGTTCTGG" 
COMMENT              "FOR primer which binds to the 5' terminus of the human H1 promoter" 
COMMENT              0 (CStringList "SENSE_PRIMER") 0) 
COMMENT         #26=(COligo "CAG2-F1" "CGTCAATAATGACGTATGTTCCC" "" 0 (CStringList) 0) 
COMMENT         #27=(COligo "CAG2-F2" "TCCGGGCTGTAATTAGCGCTTGG" "" 0 (CStringList) 0) 
COMMENT         #28=(COligo "canB-Gus 3F2" "GAACATAACAGACAAAATTGATCAGATTATTCATG" 
COMMENT              "This FOR primer binds near the 3' terminus of the Ebola glycoprotein cDNA & reads downstream of the gene" 
COMMENT              0 (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #29=(COligo "canB-Gus 3F3" "GAAGAAGTTCCAGCTAATGAAGGAACATGTC" 
COMMENT              "This FOR primer binds in the viral env gene sequence & reads forward from there." 
COMMENT              0 (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #30=(COligo "canB-Gus-1F" "GCCCTCAGGATGTCTCGGGGGCCGGCGGG" 
COMMENT              "This FOR primer targets the extreme 5' terminus of the canine beta-glucuronidase gene and appends a Bsu36I restriction site upstream of the ATG" 
COMMENT              0 (CStringList "PCR_PRIMER") 0) 
COMMENT         #31=(COligo "canB-Gus-1R" "GCCCTGAGGTCAGAGGGCGAACGGGCTGT" 
COMMENT              "This REV primer targets the extreme 3' terminus of the canine beta-glucuronidase gene and appends a Bsu36I restriction site downstream of the stop codon" 
COMMENT              0 (CStringList "PCR_PRIMER") 0) 
COMMENT         #32=(COligo "canB-Gus-2F" "GATCTCATGAATGTCTCGGGGGCCGGCGGG" 
COMMENT              "This FOR primer binds the extreme 5' terminus of the canine Beta-Gus gene & appends a BspHI site" 
COMMENT              0 (CStringList "SENSE_PRIMER" "PCR_PRIMER") 0) 
COMMENT         #33=(COligo "canB-Gus-2R" "GATCAAGCTTTCAGAGGGCGAACGGGCTGT" 
COMMENT              "This REV primer binds at the 3' terminus of the canine Beta-Glucuronidase gene & appends a HindIII site" 
COMMENT              0 (CStringList "ANTISENSE_PRIMER" "PCR_PRIMER") 0) 
COMMENT         #34=(COligo "canB-GUS-3F" "GATCTCTAGAATGTCTCGGGGGCCGGCGGG" 
COMMENT              "This FOR primer binds the extreme 5' terminus of the canine Beta-glucuronidase gene & appends an XbaI site" 
COMMENT              0 (CStringList "SENSE_PRIMER" "PCR_PRIMER") 0) 
COMMENT         #35=(COligo "canB-GUS-MF" "CACTGGGCTGGTGGATTACCAGATT" 
COMMENT              "This FOR primer binds near the middle of the canine Beta-Glucuronidase gene" 
COMMENT              0 (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #36=(COligo "canGUSB-5R" "TCCCGCGACGGGCTCTCCCG" 
COMMENT              "This REV primer binds near the 5' terminus of the canine Beta-Glucuronidase gene" 
COMMENT              0 (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #37=(COligo "canGUSB-5R2" "AGAGGCCGTCGAGGTCCTTG" 
COMMENT              "This REV primer binds near the 5' terminus of the canine Beta-glucuronidase gene" 
COMMENT              0 (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #38=(COligo "CB-R" "TGCCGCCGCACCTCTCCGCCTCG" "for pAAVEnhCB-eGFP" 0 
COMMENT              (CStringList) 0) 
COMMENT         #39=(COligo "CBA NdeI-F" "ACGGTAAACTGCCCACTTGGCAG" "" 0 (CStringList) 0) 
COMMENT         #40=(COligo "CBAsc MluI-F" "GTAGCCATGCTCTAGGAAGATC" "" 0 (CStringList) 
COMMENT              0) 
COMMENT         #41=(COligo "CBATT-13F" "AACCACCACACCCGCCGCGCTTAAT" 
COMMENT              "FOR primer against the 3' terminus of the f1 origin of replication" 
COMMENT              0 (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #42=(COligo "CBATT-14F" "GGACTGAAGCTGCTGGGGCCATGTT" 
COMMENT              "FOR primer which binds the 3' terminus of the human alpha1-antitrypsin gene" 
COMMENT              0 (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #43=(COligo "CBATT-14R" "CTCTCCCCGCGCGTTGGCCGATTCA" 
COMMENT              "REV primer which binds immediately downstream of the 3' ITR in pAAV-CB-a1AT" 
COMMENT              0 (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #44=(COligo "CBrhCG-2F" "GCTCCTCAGTGGATGTTGCCTTTAC" 
COMMENT              "FOR primer which binds to the 3' terminus of the CMV promoter" 0 
COMMENT              (CStringList "SENSE_PRIMER" "PCR_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #45=(COligo "CBrhCG-2R" "AGCAGCAGCCACAGCAGCAGCCCCT" 
COMMENT              "REV primer which binds to the 5' terminus of the rhesus chorionic gonadotropin gene" 
COMMENT              0 (CStringList) 0) 
COMMENT         #46=(COligo "CBrhCG-3F" "GGCTACTGTCCCACCATGATGCGGG" 
COMMENT              "FOR primer which binds within the rhesus chorionic gonadotropin gene" 
COMMENT              0 (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #47=(COligo "CBrhCG-3R" "GGCAACTAGAAGGCACAGTCGAGGC" 
COMMENT              "REV primer which binds the 5' terminus of the BGH polyA signal" 0 
COMMENT              (CStringList) 0) 
COMMENT         #48=(COligo "CBrhCG-4F" "AGGGGCTGCTGCTGTGGCTGCTGCT" 
COMMENT              "FOR primer which binds to the 5' terminus of the rhesus chorionic gonadotropin gene" 
COMMENT              0 (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #49=(COligo "CBrhCG-4R" "GTCAATAGGGGGCGTACTTGGCATA" 
COMMENT              "REV primer which binds the 5' terminus of the chicken beta-actin promoter (CB) with 1bp mismatch" 
COMMENT              0 (CStringList) 0) 
COMMENT         #50=(COligo "CBrhCG-5F" "GCCTCGACTGTGCCTTCTAGTTGCC" 
COMMENT              "FOR primer which binds just downstream of the rhCG gene in pAAV-EnhCB-rhCG" 
COMMENT              0 (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #51=(COligo "CFTR F" "GCTGGAATGCCAACAATTTTTGG" "" 0 (CStringList) 0) 
COMMENT         #52=(COligo "CFTR R" "CTCAAAATTGGTCTGGTCCAGCTG" "" 0 (CStringList) 0) 
COMMENT         #53=(COligo "CFTR UF BsrGI" "TTTATTGCTCCAAGAGAGTCATACC" "" 0 
COMMENT              (CStringList) 0) 
COMMENT         #54=(COligo "CFTR-3F" "CCCAGATTGCTGCTCTGAAAGAGGA" 
COMMENT              "This FOR primer binds at the 3' terminus of the human cystic fibrosis transmembrane conductance regulator gene & reads forward from there" 
COMMENT              0 (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #55=(COligo "CFTR-3R" "TCCTCTTTCAGAGCAGCAATCTGGG" 
COMMENT              "This REV primer binds at the 3' terminus of the human cystic fibrosis transmembrane conductance regulator gene & reads backwards from there" 
COMMENT              0 (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #56=(COligo "CFTR-5F" "CCCGAGAGACCATGCAGAGGTCGCC" 
COMMENT              "This FOR primer binds at the 5' terminus of the human cystic fibrosis transmembrane conductance regulator gene & reads forward from there" 
COMMENT              0 (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #57=(COligo "CFTR-5R" "GGCGACCTCTGCATGGTCTCTCGGG" 
COMMENT              "This REV primer binds at the 5' terminus of the human cystic fibrosis transmembrane conductance regulator gene & reads backwards from there" 
COMMENT              0 (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #58=(COligo "CFTR-UF 1" "TATAAAAAGCGAAGCGCGCGG" "" 0 (CStringList) 0) 
COMMENT         #59=(COligo "CFTR508" "CTTCTAATGGTGATGACAGCCTCTTCTTC" "" 0 
COMMENT              (CStringList) 0) 
COMMENT         #60=(COligo "CI intron-F" "TTCGGCTTCTGGCGTGTGACC" 
COMMENT              "For Chicken beta intron 3' end" 0 (CStringList) 0) 
COMMENT         #61=(COligo "cmCG-F" "ATGGAGATGCTCCAGGGGCTGCTG" 
COMMENT              "FOR primer against cynomologous choroionic gonadotropin cDNA sequence" 
COMMENT              0 (CStringList "SENSE_PRIMER" "PCR_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #62=(COligo "cmCG-R1" "TTATTGCGGGAGGAACGGGGTGTC" 
COMMENT              "REV primer against cynomologous chorionic gonadotropin gene" 0 
COMMENT              (CStringList "ANTISENSE_PRIMER" "PCR_PRIMER" "SEQUENCING_PRIMER") 
COMMENT              0) 
COMMENT         #63=(COligo "cmCG-R2" "TTATTGCGGGAGGAACGGGTTGTC" 
COMMENT              "REV primer against cynomologous chorionic gonadotropin gene" 0 
COMMENT              (CStringList "ANTISENSE_PRIMER" "PCR_PRIMER" "SEQUENCING_PRIMER") 
COMMENT              0) 
COMMENT         #64=(COligo "CMV-F" "TGCTAACGCAGTCAGTGCTT" 
COMMENT              "FOR Primer against 3' terminus of CMV promoter" 0 
COMMENT              (CStringList "SENSE_PRIMER" "PCR_PRIMER") 0) 
COMMENT         #65=(COligo "CMV-MR" "GCATCACCATGGTAATAGCGATGAC" 
COMMENT              "This REV primer binds in the middle of the CMV promoter & reads upstream" 
COMMENT              0 (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #66=(COligo "CMV-R" "TGTTGTGTCAGAAGCACTGA" 
COMMENT              "REV Primer against 3' terminus of CMV promoter" 0 
COMMENT              (CStringList "ANTISENSE_PRIMER") 0) 
COMMENT         #67=(COligo "CMVEnh-F" "ATCATATGCCAAGTCCGCCC" 
COMMENT              "This FOR primer binds the 3' terminus of the CMV enhancer" 0 
COMMENT              (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #68=(COligo "CMVEnh-R" "AGGGGGCGTACTTGGCATATGATAC" 
COMMENT              "This REV primer binds near the middle of the CMV enhancer" 0 
COMMENT              (CStringList) 0) 
COMMENT         #69=(COligo "Copy of canB-Gus-2F" "ATGAATGTCTCGGGGGCCGGCGGG" 
COMMENT              "This FOR primer binds the extreme 5' terminus of the canine Beta-Gus gene" 
COMMENT              0 (CStringList) 0) 
COMMENT         #70=(COligo "cPPT-F" "GGTACAGTGCAGGGGAAAGAATAGT" 
COMMENT              "FOR primer which binds to the 3' terminus of the central copy of the polypurine tract (cPPT)" 
COMMENT              0 (CStringList "SENSE_PRIMER" "PCR_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #71=(COligo "cynoA1AT-Rv2" 
COMMENT              "CGGCGCGCCCACCCTTCAGTTAGAATTCTTCTTTCTGGGTGGG" 
COMMENT              "REV Primter against cynomologous alpha1-antitrypsin gene (version 2)" 
COMMENT              0 (CStringList) 0) 
COMMENT         #72=(COligo "EboZ-3F" "TTGGAGTTACAGGCGTTATAATTGC" 
COMMENT              "This FOR primer binds at the 3' terminus of the Ebola glycoprotein gene" 
COMMENT              0 (CStringList) 0) 
COMMENT         #73=(COligo "EboZ-3R" "CTAAAAGACAAATTTGCATATACAGAATAA" 
COMMENT              "This REV primer binds at the 3' terminus of the Ebola glycoprotein gene" 
COMMENT              0 (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #74=(COligo "EboZ-5F" "ATGGGCGTTACAGGAATATTGCAGT" 
COMMENT              "This FOR primer binds to the 5' terminus of the Ebola glycoprotein gene" 
COMMENT              0 (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #75=(COligo "EboZ-5R" "TTACCCAAAGAAAGAATGATGTCCT" 
COMMENT              "This REV primer binds to the 5' terminus of the Ebola glycoprotein gene" 
COMMENT              0 (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #76=(COligo "EboZ-MF" "TTCAGCTCACACCCCTTGAGAGAGC" 
COMMENT              "This FOR primer binds near the middle of the Ebola glycoprotein gene" 
COMMENT              0 (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #77=(COligo "Eco1" "GATCGATATCATTTCCCCGAAAAGTGCCAC" "" 0 (CStringList) 
COMMENT              0) 
COMMENT         #78=(COligo "Eco2" "GATCGATATCTGGCCGATTCATTAATGCAG" "" 0 (CStringList) 
COMMENT              0) 
COMMENT         #79=(COligo "eGFP-3Ra" "GATCCCATGGGCACTAGTCTTGTACAGCTCGTCCATGCCGAG" 
COMMENT              "This REV primer binds at the extreme 3' terminus of the eGFP gene & appends 2bp + an NcoI site immediately upstream of the stop codon." 
COMMENT              0 (CStringList "ANTISENSE_PRIMER" "PCR_PRIMER") 0) 
COMMENT         #80=(COligo "eGFP-5Fa" "GATCAAGCTTATGGTGAGCAAGGGCGAGGAGCTGT" 
COMMENT              "This FOR primer binds at the extreme 5' terminus of the eGFP gene & appends a HindIII site" 
COMMENT              0 (CStringList "SENSE_PRIMER" "PCR_PRIMER") 0) 
COMMENT         #81=(COligo "eGFP-5R" "TTACGTCGCCGTCCAGCTCG" 
COMMENT              "This REV primer binds at the 5' terminus of the enhanced GFP gene" 
COMMENT              0 (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #82=(COligo "eGFP-F" "CATGGTCCTGCTGGAGTTCGTGACC" "" 0 (CStringList) 0) 
COMMENT         #83=(COligo "eGFP-ilnker F" "GTACAAGACTAGTTAATAAGC" 
COMMENT              "FOR primer which forms a LINKER with the eGFP-linker R and bridges the gap between BsrGI-cut eGFP and a downstream MfeI site." 
COMMENT              0 (CStringList "SENSE_PRIMER") 0) 
COMMENT         #84=(COligo "eGFP-linker R" "AATTGCTTATTAACTAGTCTT" 
COMMENT              "REV primer which forms a LINKER with the eGFP-linker F oligo and bridges the gap between BsrGI-cut eGFP and a downstream MfeI site." 
COMMENT              0 (CStringList) 0) 
COMMENT         #85=(COligo "eGFP-R" "TCAGCTTGCCGTAGGTGGCATC" "" 0 (CStringList) 0) 
COMMENT         #86=(COligo "Env-MF" "GCAGAACCGAGCGGCTATTGACTTC" 
COMMENT              "This FOR primer binds near the middle of the viral env gene" 0 
COMMENT              (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #87=(COligo "ENV-MF2" "GGGGTGGAGGGGGGTGGTATGGAGC" 
COMMENT              "This FOR primer binds within the viral env gene sequence" 0 
COMMENT              (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #88=(COligo "gt 10 Forward" "CCTTTTGAGCAAGTTCAGCCTGG" 
COMMENT              "Upenn cell center has it" 0 (CStringList) 0) 
COMMENT         #89=(COligo "gt 10 Reverse" "GGTGGCTTATGAGTATTTCTTCC" 
COMMENT              "Upenn cell center has it" 0 (CStringList) 0) 
COMMENT         #90=(COligo "gt 11 Forward" "ATTGGTGGCGACGACTCCTGGAG" 
COMMENT              "Upenn cell center has it" 0 (CStringList) 0) 
COMMENT         #91=(COligo "gt 11 Reverse" "CAGACCAACTGGTAATGGTAGC" 
COMMENT              "Upenn cell center has it" 0 (CStringList) 0) 
COMMENT         #92=(COligo "H1R-BamHI" "GATGAATTCGGATCCGAGTGGTCTCATACAGAACTTATAA" 
COMMENT              "REV primer which binds the 3' terminus of the human H1 promoter and also introduced a downstream BamHI site" 
COMMENT              0 (CStringList "ANTISENSE_PRIMER") 0) 
COMMENT         #93=(COligo "H5-F" "CTGTAAACGCCACCGTCTTCACC" "For pH5' 000-Spe I, " 0 
COMMENT              (CStringList) 0) 
COMMENT         #94=(COligo "H5-R" "GCGCAAGCATGCCATTGGAGG" 
COMMENT              "For pH5' 000-Spe I, hybrid H5 fiber" 0 (CStringList) 0) 
COMMENT         #95=(COligo "hApoA1-3R1" "CCCACTTTGGAAACGTTTAT" 
COMMENT              "This REV primer binds the 3' terminus of the human Apoliproprotein A1 gene" 
COMMENT              0 (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #96=(COligo "hApoA1-5F1" "CCTCCCAGCCCAGACCCTGG" 
COMMENT              "This FOR primer binds at the 5' terminus of one of the mRNA's for the human Apolipoprotein A1 gene" 
COMMENT              0 (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #97=(COligo "hApoA1-5F2" "ATGAAAGCTGCGGTGCTGAC" 
COMMENT              "This FOR primer binds the 5' terminus of the human Apolipoprotein A1 gene" 
COMMENT              0 (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #98=(COligo "hApoA1-MF1" "ATGCGCTGGAGGCATAGAAG" 
COMMENT              "This FOR primer binds at the 5' terminus of one of the mRNA's for the human Apolipoprotein A1 gene" 
COMMENT              0 (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #99=(COligo "hApoA1-MR1" "CTTCTATGCCTCCAGCGCAT" 
COMMENT              "This REV primer binds in the middle of the human Apolipoprotein A1 gene" 
COMMENT              0 (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #100=(COligo "HIVgag-5F" "TCGAGATCAAGGACACCAAGGAAGC" 
COMMENT               "This FOR primer binds (kind of) near the 5' end of the HIV gag 55 gene." 
COMMENT               0 (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #101=(COligo "HIVgag-F" "ACCATCCTGAAGGCTCTCGG" 
COMMENT               "This FOR primer binds the 3' terminus of the HIV gag gene" 0 
COMMENT               (CStringList) 0) 
COMMENT         #102=(COligo "HRcPPT-19F" "CTGCTGACATCGAGCTTGCTACAAG" 
COMMENT               "FOR primer binding within the HIV 3' LTR of pHRcPPT-WP" 0 
COMMENT               (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #103=(COligo "HRcPPT-20R" "ATCACAATTTGTCAAAACAAGTTTG" 
COMMENT               "FOR primer which binds just downstream of the 3' LTR in pHRcPPT-WP" 
COMMENT               0 (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #104=(COligo "hRep-1F" "GCGCTAGCATGGCGGATACTCTCCCTTC" 
COMMENT               "This FOR primer targets the extreme 5' terminus of the human Rep-1 gene & appends an NheI recognition site upstream of the ATG" 
COMMENT               0 (CStringList "PCR_PRIMER" "SENSE_PRIMER") 0) 
COMMENT         #105=(COligo "hRep-1R" "AGCGGCCGCTTATTCAGAGGACTCCTCTA" 
COMMENT               "This REV primer targets the extreme 3' terminus of the human Rep-1 gene & appends a NotI recognition site downstream of the stop codon" 
COMMENT               0 (CStringList "PCR_PRIMER" "ANTISENSE_PRIMER") 0) 
COMMENT         #106=(COligo "ICeuI-F" "GGGGTTCCGCGCACATTTCC" 
COMMENT               "This FOR primer binds 21bp upstream of the I-CeuI restriction site in pShuttle (Clontech)" 
COMMENT               0 (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #107=(COligo "iNOS Bsu36I-R" "ATGGAGTCTGCAGCACTTGG" "" 0 (CStringList) 
COMMENT               0) 
COMMENT         #108=(COligo "IRES-R" "CCAAGCGGCTTCGGCCAGTAACG" "" 0 (CStringList) 0) 
COMMENT         #109=(COligo "ITR3F" 
COMMENT               "GGGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGTAGCCATGCTCTAGGAA" 
COMMENT               "1 of 4 oligo's designed to form an entire AAV ITR, with the terminal resolution site (TRS) deleted, to induce a self-complimentary form following second-strand synthesis, in vivo." 
COMMENT               0 (CStringList "SENSE_PRIMER") 0) 
COMMENT         #110=(COligo "ITR3R'" 
COMMENT               "GATCTTCCTAGAGCATGGCTACACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCA" 
COMMENT               "1 of 4 oligo's designed to form an entire AAV ITR, with the terminal resolution site (TRS) deleted, to induce a self-complimentary form following second-strand synthesis, in vivo." 
COMMENT               0 (CStringList "ANTISENSE_PRIMER") 0) 
COMMENT         #111=(COligo "ITR5F'" 
COMMENT               "ATTAGGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTC" 
COMMENT               "1 of 4 oligo's designed to form an entire AAV ITR, with the terminal resolution site (TRS) deleted, to induce a self-complimentary form following second-strand synthesis, in vivo." 
COMMENT               0 (CStringList "SENSE_PRIMER") 0) 
COMMENT         #112=(COligo "ITR5R" 
COMMENT               "AAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCAGCCTAA" 
COMMENT               "1 of 4 oligo's designed to form an entire AAV ITR, with the terminal resolution site (TRS) deleted, to induce a self-complimentary form following second-strand synthesis, in vivo." 
COMMENT               0 (CStringList "ANTISENSE_PRIMER") 0) 
COMMENT         #113=(COligo "LacZ-BsiWI" "AAGAAAACTATCCCGACCGCC" "" 0 (CStringList) 0) 
COMMENT         #114=(COligo "M13 forward" "CCCAGTCACGACGTTGTAAAACG" "" 0 (CStringList) 
COMMENT               0) 
COMMENT         #115=(COligo "M13 Reverse" "AGCGGATAACAATTTCACACAGG" "" 0 (CStringList) 
COMMENT               0) 
COMMENT         #116=(COligo "mEpo-F" "GTCGCCTCCAGATACCACCCCACC" "" 0 (CStringList) 0) 
COMMENT         #117=(COligo "mOPN-R1" "TCTGAGCTGCCAGAATCAGTCAC" "" 0 (CStringList) 0) 
COMMENT         #118=(COligo "mPTP 1B-F" "TGGAGGACAGCTCTTCCTCGG" 
COMMENT               "for mouse PTP-1B sequence" 0 (CStringList) 0) 
COMMENT         #119=(COligo "mRep-1F" "GCGCTAGCATGGCGGATAATCTCCCTTC" 
COMMENT               "This FOR primer targets the extreme 5' terminus of the mouse Rep-1 gene & appends an NheI site upstream of the ATG" 
COMMENT               0 (CStringList "PCR_PRIMER") 0) 
COMMENT         #120=(COligo "mRep-1R" "AGCGGCCGCCTATTCAGAGGGCTCTTCTG" 
COMMENT               "This REV primer targets the extreme 3' terminus of the mouse Rep-1 gene & appends a NotI restriction site" 
COMMENT               0 (CStringList) 0) 
COMMENT         #121=(COligo "Nef1" "ATGGGCGGCGCAATTTCCATGAGG" 
COMMENT               "FOR Primer against 5' terminus of HIV nef cDNA" 0 
COMMENT               (CStringList "SENSE_PRIMER" "PCR_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #122=(COligo "Nef2" "TCATCTGGTCTCTTTCTTATCGGC" 
COMMENT               "REV Primer against 3' terminus of HIV nef cDNA" 0 
COMMENT               (CStringList "ANTISENSE_PRIMER" "PCR_PRIMER" "SEQUENCING_PRIMER") 
COMMENT               0) 
COMMENT         #123=(COligo "pAAVCBa1AT-10F" "TCCCTTAACGTGAGTTTTCG" 
COMMENT               "REV primer binds downstream of the pUC19 origin of replication in pAAV-CB-a1AT" 
COMMENT               0 (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #124=(COligo "pAAVCBa1AT-11F" "GTCGATTTTTGTGATGCTCG" 
COMMENT               "REV primer which binds at the 3' terminus of the pUC19 origin of replication" 
COMMENT               0 (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #125=(COligo "pAAVCBa1AT-12F" "TGTAGTTAATGATTAACCCGCC" 
COMMENT               "REV primer binds between the SV40 polyA signal and the 3' ITR in pAAV-CB-a1AT" 
COMMENT               0 (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #126=(COligo "pAAVCBa1AT-1F" "CGCGTTGGCCGATTCATTAA" 
COMMENT               "REV primer immediately downstream of the 3' ITR of pAAV-CB-a1AT" 
COMMENT               0 (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #127=(COligo "pAAVCBa1AT-2F" "ACCACTTTTCCCATGAAGAG" 
COMMENT               "REV primer which binds near the 3' terminus of the human alpha1-antitrypsin gene" 
COMMENT               0 (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #128=(COligo "pAAVCBa1AT-3F" "TGTAATTCACCAGAGCAAAA" 
COMMENT               "REV primer which binds near the middle of the human alpha1-antitrypsin gene" 
COMMENT               0 (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #129=(COligo "pAAVCBa1AT-4F" "GGATGCCCCACGAGACAGAA" 
COMMENT               "REV primer which binds near the 5' terminus of the human alpha1-antitrypsin gene" 
COMMENT               0 (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #130=(COligo "pAAVCBa1AT-5F" "ATTGACGTCAATGGGCGGGGTC" 
COMMENT               "REV primer which binds upstream of the CB promoter of pAAV-CB-a1AT" 
COMMENT               0 (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #131=(COligo "pAAVCBa1AT-6F" "CCTCCTGTTTAGCTCCCGCT" 
COMMENT               "REV primer binding between the f1 origin of replication & the 5' ITR of pAAV-CB-a1AT" 
COMMENT               0 (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #132=(COligo "pAAVCBa1AT-7F" "AGTTTTACGATTACCGTTCA" 
COMMENT               "REV primer binding upstream of the f1 origin of replication in pAAV-CB-a1AT" 
COMMENT               0 (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #133=(COligo "pAAVCBa1AT-8F" "GGTTTTCACCGTCATCACCG" 
COMMENT               "REV primer binding downstream of the Amp promoter of pAAV-CB-a1AT" 
COMMENT               0 (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #134=(COligo "pAAVCBa1AT-9F" "GCCATAACCATGAGTGATAA" 
COMMENT               "REV primer which binds in the middle of the beta-lactamase (Amp res) gene" 
COMMENT               0 (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #135=(COligo "pGEM4-F1" "TGCGGGACGGCGTTACCAG" "" 0 (CStringList) 0) 
COMMENT         #136=(COligo "PGK2-F" "TCCACTACATGACCCTCTGCCC" 
COMMENT               "For PGK2 promoter, the hybridizition site in the middle of PGK2" 
COMMENT               0 (CStringList) 0) 
COMMENT         #137=(COligo "PGK2-R" "TCCTGGGCAGAGGGTCATGTAGTG" 
COMMENT               "The hybridizition site is in the middle of PGK2" 0 (CStringList) 
COMMENT               0) 
COMMENT         #138=(COligo "pHRcPPT-10F" "AAGTTGCAGGACCACTTCTG" 
COMMENT               "FOR primer binding to the pHRcPPT-WP plasmid backbone." 0 
COMMENT               (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #139=(COligo "pHRcPPT-11F" "GCTCTGCTAATCCTGTTACC" 
COMMENT               "FOR primer binding to the pHRcPPT-WP plasmid backbone" 0 
COMMENT               (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #140=(COligo "pHRcPPT-12F" "TAATGCAGCTGTGGAATGTG" 
COMMENT               "FOR primer binding to the pHRcPPT-WP plasmid backbone" 0 
COMMENT               (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #141=(COligo "pHRcPPT-13F" "ACTCGCAAGCCGACTGATGC" 
COMMENT               "FOR primer binding to the pHRcPPT-WP plasmid backbone" 0 
COMMENT               (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #142=(COligo "pHRcPPT-14F" "CAGTCGGCCCTTGATGGTAA" 
COMMENT               "FOR primer binding to the pHRcPPT-WP plasmid backbone" 0 
COMMENT               (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #143=(COligo "pHRcPPT-15F" "GCTGCACTGCTATACAAGAA" 
COMMENT               "FOR primer binding to the pHRcPPT-WP plasmid backbone" 0 
COMMENT               (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #144=(COligo "pHRcPPT-16R" "TCCCACTGCTCTTTTTTCTCTCTGC" 
COMMENT               "REV primer binding between the packaging signal & cPPT of pHRcPPT-WP" 
COMMENT               0 (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #145=(COligo "pHRcPPT-17R" "TGTCGTTAGAACGCGGCTACAATTA" 
COMMENT               "REV primer binding downstream of the 3' LTR in pHRcPPT-WP" 0 
COMMENT               (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #146=(COligo "pHRcPPT-18F" "GCGCCCTGACGGGCTTGTCTGCTCC" 
COMMENT               "FOR primer binding to the pHRcPPT-WP plasmid backbone" 0 
COMMENT               (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #147=(COligo "pHRcPPT-18R" "AGGATCTTACCGCTGTTGAGATCCA" 
COMMENT               "REV primer binding to the pHRcPPT-WP plasmid backbone" 0 
COMMENT               (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #148=(COligo "pHRcPPT-1F" "TTGTATTTGTTAAATATGTACTACA" 
COMMENT               "FOR primer binding immediately upstream of the 5' LTR in pHRcPPT-WP" 
COMMENT               0 (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #149=(COligo "pHRcPPT-2F" "TGGTAACTAGAGATCCCTCA" 
COMMENT               "FOR primer binding at the 3' terminus of the 5' HIV LTR in pHRcPPT-WP" 
COMMENT               0 (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #150=(COligo "pHRCPPT-3F" "GAGGGACAATTGGAGAAGTG" 
COMMENT               "FOR primer binding between the packaging signal & cPPT in pHRcPPT-WP" 
COMMENT               0 (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #151=(COligo "pHRcPPT-4F" "AACAAATTGGCTGTGGTATA" 
COMMENT               "FOR primer binding upstream of cPPT in pHRcPPT-WP" 0 
COMMENT               (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #152=(COligo "pHRcPPT-5F" "AACAAATTGGCTGTGGTATA" 
COMMENT               "FOR primer binding in the middle of the WPRE (woodchuck regulatory element)" 
COMMENT               0 (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #153=(COligo "pHRcPPT-6F" "ACCAGGGCCAGGGATCAGAT" 
COMMENT               "FOR primer which binds near the 5' terminus of the HIV 3' LTR" 0 
COMMENT               (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #154=(COligo "pHRcPPT-7F" "GCATCTAGAATTAATTCCGTGT" 
COMMENT               "FOR primer binding to the pHRcPPT-WP plasmid backbone" 0 
COMMENT               (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #155=(COligo "pHRcPPT-8F" "AAGATCGGGCTCGCCACTTC" 
COMMENT               "FOR primer binding to the pHRcPPT-WP plasmid backbone" 0 
COMMENT               (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #156=(COligo "pHRcPPT-9F" "GCGGCATTTTGCCTTCCTGT" 
COMMENT               "FOR primer binding to the pHRcPPT-WP plasmid backbone" 0 
COMMENT               (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #157=(COligo "PI-F" "TGCGTTTCTGATAGGCACCTATTG" 
COMMENT               "stick to promega intron" 0 (CStringList) 0) 
COMMENT         #158=(COligo "PI-SceI-R" "CCCATAATGCCATTTCATTACCTCTTTCTC" "" 0 
COMMENT               (CStringList) 0) 
COMMENT         #159=(COligo "pIRESpuro-F" "CCCACTGCTTACTGGCTTATCGAAA" 
COMMENT               "FOR primer binding between CMV promoter and EF1-alpha promoter of pCMV-EF1-hGDNF-IRESpuro3" 
COMMENT               0 (CStringList "SENSE_PRIMER") 0) 
COMMENT         #160=(COligo "pIRESpuro-R" "ATCCGAATTCGAATCCGGAGACGTA" 
COMMENT               "REV primer binding between the hGDNF gene & the IVS intron of pCMV-EF1-hGDNF-IRESpuro3" 
COMMENT               0 (CStringList "ANTISENSE_PRIMER") 0) 
COMMENT         #161=(COligo "PRM1" "AACGCAGTCAGGCACCGTGT" 
COMMENT               "Sense primer for PCR amplification of 86 - 1286 region of PBR322" 
COMMENT               0 (CStringList "SENSE_PRIMER" "PCR_PRIMER") 0) 
COMMENT         #162=(COligo "PRM2" "ATCCGTTAGCGAGGTGCCGC" 
COMMENT               "Antisense primer for PCR amplification of 86 - 1286 region of PBR322" 
COMMENT               0 (CStringList "PCR_PRIMER" "ANTISENSE_PRIMER") 0) 
COMMENT         #163=(COligo "RFP-F" "CTGCACTGCTCCTTCAAGACCAC" "for turbo RFP" 0 
COMMENT               (CStringList) 0) 
COMMENT         #164=(COligo "RGP-R" "TGCAATGAAAATAAATTTCCTTTATTAGCC" 
COMMENT               "Reverse reading for Rabbit globin poly A" 0 (CStringList) 0) 
COMMENT         #165=(COligo "rhAFP-F1" "TCGCTATGACAAAATAATTCCATC" "" 0 (CStringList) 0) 
COMMENT         #166=(COligo "rhAFP-F2" "TTACAGAAATACATCCAGGAGAGCC" "" 0 (CStringList) 
COMMENT               0) 
COMMENT         #167=(COligo "rhAFP-F3" "AGTCTGCTTTGCTGAAGAGGG" "" 0 (CStringList) 0) 
COMMENT         #168=(COligo "rhCG-F" "CTCCCCCCAGCCCTCCAAGTCCATC" 
COMMENT               "FOR primer which binds to the 3' terminus of the rhesus chorionic gonadotropin gene" 
COMMENT               0 (CStringList "SENSE_PRIMER" "PCR_PRIMER") 0) 
COMMENT         #169=(COligo "Sp6" "ATTTAGGTGACACTATAGAA" "Upenn cell center has it" 0 
COMMENT               (CStringList) 0) 
COMMENT         #170=(COligo "SV40-F" "TTCAGGGGGAGATGTGGGAGGT" 
COMMENT               "FOR Primer against 3' terminus of SV40 polyA signal" 0 
COMMENT               (CStringList "SENSE_PRIMER" "PCR_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #171=(COligo "SV40-R" "TCATGTCTGCTCGAAGCGGC" 
COMMENT               "REV Primer against 5' terminus of SV40 polyA signal" 0 
COMMENT               (CStringList "ANTISENSE_PRIMER" "PCR_PRIMER" "SEQUENCING_PRIMER") 
COMMENT               0) 
COMMENT         #172=(COligo "Syn-3R" "GATCGAATTCTCTAGAGGATCCCCGTCCTCGAGTG" 
COMMENT               "This REV primer binds downstream of the SYN promoter & appends an EcoRI site to the PCR fragment" 
COMMENT               0 (CStringList "ANTISENSE_PRIMER" "PCR_PRIMER") 0) 
COMMENT         #173=(COligo "T3 promoter/primer" "ATTAACCCTCACTAAAGGGA" 
COMMENT               "Upenn cell center has it" 0 (CStringList) 0) 
COMMENT         #174=(COligo "T7 promoter/primer" "TAATACGACTCACTATAGGG" 
COMMENT               "Upenn cell center has it" 0 (CStringList) 0) 
COMMENT         #175=(COligo "T7 terminator" "GCTAGTTATTGCTCAGCGG" 
COMMENT               "Upenn cell center has it" 0 (CStringList) 0) 
COMMENT         #176=(COligo "TBG-F 2" "CTGAAGACACTCTTGCCAGCA" 
COMMENT               "FOR Primer against the 3' terminus of the TBG promoter" 0 
COMMENT               (CStringList "SENSE_PRIMER" "PCR_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #177=(COligo "TBG-MR" "AAAGAGGATTGTCAGAGCTGGAGGG" 
COMMENT               "This REV primer binds near the middle of the thyroxin-binding globulin (TBG) promoter" 
COMMENT               0 (CStringList "ANTISENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #178=(COligo "temp" "GAAGAAGTTCCAGCTAATGAAGGAACATGTC" "" 0 
COMMENT               (CStringList) 0) 
COMMENT         #179=(COligo "U6-F" "TAGCCATGCTCTAGGAAGATCTGGG" 
COMMENT               "FOR primer which binds the 3' terminus of the human U6 promoter" 
COMMENT               0 (CStringList "SENSE_PRIMER" "SEQUENCING_PRIMER") 0) 
COMMENT         #180=(COligo "U6-F1" "ACGTGACGTAGAAAGTAATAATTTCTTGGG" "" 0 
COMMENT               (CStringList) 0) 
COMMENT         #181=(COligo "U6-R1" "TACGTCATTATTGACGTCAATGGGC" "" 0 (CStringList) 0) 
COMMENT         #182=(COligo "VMD F1" "TGTCAAAGACCCCAGGGCCTAG" "" 0 (CStringList) 0) 
COMMENT         #183=(COligo "VMD F2" "TTTGAGGCCTTCAGGGTTGG" "" 0 (CStringList) 0) 
COMMENT         #184=(COligo "VMD F3" "CAGGTTGGATGGTTGGGAACATCC" "" 0 (CStringList) 0) 
COMMENT         #185=(COligo "VMD F4" "GATTACAGGCACCCGCACCA" "" 0 (CStringList) 0) 
COMMENT         #186=(COligo "VMD R" "GTTCTGACCCCTGAGGCCTTGCA" "" 0 (CStringList) 0) 
COMMENT         #187=(COligo "WPRE-F" "TCCTTTCCTTGGCTGCTCGC" "" 0 (CStringList) 0) 
COMMENT         #188=(COligo "WPRE-R" "GTTGGGAGTGAATTAGCCCTTCCAG" 
COMMENT               "REV primer which binds to the 5' terminus of the Woodchuck hepatitis Post-transcriptional Regulatory Element" 
COMMENT               0 
COMMENT               (CStringList "ANTISENSE_PRIMER" "PCR_PRIMER" "SEQUENCING_PRIMER") 
COMMENT               0)) 1 "{(0,1),2}" 0 0 "" 0 4294967295 1 0 0 0 0 0 1 "MAIN" 0 0 30 
COMMENT        0 (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5342 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #189=(CRSite (CStringList) "XmaI" "cccggg" 2 0 5 30 0 41 0 2135 0 2478 0 
COMMENT              2489 0 0 "") 
COMMENT        #190=(CRSite (CStringList) "NspI" "rcatgy" 6 0 4 206 0 1160 0 1361 0 
COMMENT              4964 0 0 "")) 
COMMENT       (CObList 
COMMENT        #191=(CFSignal (CObList) "Enhancer" 9 0 0 212 472 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "212..472") 
COMMENT        #192=(CFSignal (CObList) "TATA" 41 0 0 674 677 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "674..677") 
COMMENT        #193=(CFSignal (CObList) "CMV promoter" 29 0 0 473 700 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "473..700") 
COMMENT        #194=(CFSignal (CObList) "f1 ori" 33 0 1 2698 3153 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "complement(2698..3153)") 
COMMENT        #195=(CFSignal (CObList) "AP(R)" 22 0 0 3284 4141 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "3284..4141") 
COMMENT        #196=(CFSignal (CObList) "Origin of replication" 33 0 0 4315 4903 0 
COMMENT              (CStringList) (CStringList) 1 1 1 1 "4315..4903") 
COMMENT        #197=(CFSignal (CObList) "3' ITR" 34 0 1 2392 2521 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "complement(2392..2521)") 
COMMENT        #198=(CFSignal (CObList) "5' ITR" 34 0 0 1 130 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "1..130") 
COMMENT        #199=(CFSignal (CObList) "Rabbit globin poly A" 25 0 0 2177 2303 0 
COMMENT              (CStringList) (CStringList) 1 1 1 1 "2177..2303") 
COMMENT        #200=(CFSignal (CObList) "Intron_1" 15 0 0 824 956 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "824..956") 
COMMENT        #201=(CFSignal (CObList) "Cre" 4 0 0 1070 2101 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "1070..2101")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #202=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT              (CObjectList 
COMMENT               #203=(CRefLinePar 
COMMENT                     (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) 
COMMENT                      "pENN AAV.CMV.PI.Cre.rBG(p1090-R)" 2) 5 "" 0 4) 
COMMENT               #204=(CFolderPar 
COMMENT                     (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                      (CObjectList 
COMMENT                       #205=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "DNA 'pENN AAV.CMV.PI.Cre.rBG(p1090-R)'" 1) 
COMMENT                       #206=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Currently local object. Original author: Xiangyang Zhou" 
COMMENT                             1) 
COMMENT                       #207=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Created: 10/21/17 08:40AM" 1) 
COMMENT                       #208=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Last Modified: 10/21/17 08:40AM" 1) 
COMMENT                       #209=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "length: 5342 bp" 1) 
COMMENT                       #210=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "storage type: Basic" 1) 
COMMENT                       #211=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "form: Circular" 1))) "General Description") 
COMMENT               #212=(CFolderPar 
COMMENT                     (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                      (CObjectList 
COMMENT                       #213=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Original Source Database: GenBank" 1) 
COMMENT                       #214=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Modification Date in the Original DB: 27-JUN-2011" 
COMMENT                             1))) "Standard Fields") 
COMMENT               #215=(CFolderPar 
COMMENT                     (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) 
COMMENT                      (CObjectList 
COMMENT                       #216=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Xiangyang Zhou" 1) 
COMMENT                       #217=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "TEL: 215-898-7117  " 1) 
COMMENT                       #218=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "EMAIL: <a href=\"mailto:zhou3@mail.med.upenn.edu\">zhou3@mail.med.upenn.edu</a>" 
COMMENT                             1))) "Original Author") 
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COMMENT               #220=(CFolderPar 
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COMMENT                     "Annotations") 
COMMENT               #221=(CFolderPar 
COMMENT                     (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                      (CObjectList 
COMMENT                       #222=(CFolderPar 
COMMENT                             (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #223=(CFolderPar 
COMMENT                                     (CGroupPar 
COMMENT                                      (CParagraph 1070 (3 #201# 0) 1 2 2 0 327) 
COMMENT                                      (CObjectList 
COMMENT                                       #224=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Start: 1070  End: 2101" 1) 
COMMENT                                       #225=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Original Location Description:" 1) 
COMMENT                                       #226=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "   1070..2101" 1))) "Cre"))) 
COMMENT                             "CDS (1 total)") 
COMMENT                       #227=(CFolderPar 
COMMENT                             (CGroupPar (CParagraph 9 (7 9 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
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FEATURES             Location/Qualifiers
     enhancer        212..472
                     /vntifkey="9"
                     /label=Enhancer
     TATA_signal     674..677
                     /vntifkey="41"
                     /label=TATA
     promoter        473..700
                     /vntifkey="29"
                     /label=CMV\promoter
     rep_origin      complement(2698..3153)
                     /vntifkey="33"
                     /label=f1\ori
     misc_marker     3284..4141
                     /vntifkey="22"
                     /label=AP(R)
     rep_origin      4315..4903
                     /vntifkey="33"
                     /label=Origin\of\replication
     repeat_region   complement(2392..2521)
                     /vntifkey="34"
                     /label=3'\ITR
     repeat_region   1..130
                     /vntifkey="34"
                     /label=5'\ITR
     polyA_signal    2177..2303
                     /vntifkey="25"
                     /label=Rabbit\globin\poly\A
     intron          824..956
                     /vntifkey="15"
                     /label=Intron_1
                     /note="chimeric intron"
     CDS             1070..2101
                     /vntifkey="4"
                     /label=Cre
BASE COUNT     1310 a      1327 c      1353 g      1352 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag ggtaatgggg 
      181 atcctctaga actatagcta gcatgcctgc aggctgaccg cccaacgacc cccgcccatt 
      241 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      301 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      361 aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      421 catgacctta cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      481 catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg actcacgggg 
      541 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      601 ggactttcca aaatgtcgta ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt 
      661 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatca ctagaagctt 
      721 tattgcggta gtttatcaca gttaaattgc taacgcagtc agtgcttctg acacaacagt 
      781 ctcgaactta agctgcagaa gttggtcgtg aggcactggg caggtaagta tcaaggttac 
      841 aagacaggtt taaggagacc aatagaaact gggcttgtcg agacagagaa gactcttgcg 
      901 tttctgatag gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgt 
      961 ccactcccag ttcaattaca gctcttaagg ctagagtact taatacgact cactataggc 
     1021 tagcctcgag aattgtacac tttacttaaa accattatct gagtgtgaaa tgtccaattt 
     1081 actgaccgta caccaaaatt tgcctgcatt accggtcgat gcaacgagtg atgaggttcg 
     1141 caagaacctg atggacatgt tcagggatcg ccaggcgttt tctgagcata cctggaaaat 
     1201 gcttctgtcc gtttgccggt cgtgggcggc atggtgcaag ttgaataacc ggaaatggtt 
     1261 tcccgcagaa cctgaagatg ttcgcgatta tcttctatat cttcaggcgc gcggtctggc 
     1321 agtaaaaact atccagcaac atttgggcca gctaaacatg cttcatcgtc ggtccgggct 
     1381 gccacgacca agtgacagca atgctgtttc actggttatg cggcggatcc gaaaagaaaa 
     1441 cgttgatgcc ggtgaacgtg caaaacaggc tctagcgttc gaacgcactg atttcgacca 
     1501 ggttcgttca ctcatggaaa atagcgatcg ctgccaggat atacgtaatc tggcatttct 
     1561 ggggattgct tataacaccc tgttacgtat agccgaaatt gccaggatca gggttaaaga 
     1621 tatctcacgt actgacggtg ggagaatgtt aatccatatt ggcagaacga aaacgctggt 
     1681 tagcaccgca ggtgtagaga aggcacttag cctgggggta actaaactgg tcgagcgatg 
     1741 gatttccgtc tctggtgtag ctgatgatcc gaataactac ctgttttgcc gggtcagaaa 
     1801 aaatggtgtt gccgcgccat ctgccaccag ccagctatca actcgcgccc tggaagggat 
     1861 ttttgaagca actcatcgat tgatttacgg cgctaaggat gactctggtc agagatacct 
     1921 ggcctggtct ggacacagtg cccgtgtcgg agccgcgcga gatatggccc gcgctggagt 
     1981 ttcaataccg gagatcatgc aagctggtgg ctggaccaat gtaaatattg tcatgaacta 
     2041 tatccgtaac ctggatagtg aaacaggggc aatggtgcgc ctgctggaag atggcgatta 
     2101 gccattaacg cggcgtggta cctctagagt cgacccgggc ggcctcgagg acggggtgaa 
     2161 ctacgcctga ggatccgatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc 
     2221 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg 
     2281 gaattttttg tgtctctcac tcggaagcaa ttcgttgatc tgaatttcga ccacccataa 
     2341 tacccattac cctggtagat aagtagcatg gcgggttaat cattaactac aaggaacccc 
     2401 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac 
     2461 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca 
     2521 gccttaatta acctaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg 
     2581 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg 
     2641 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg 
     2701 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 
     2761 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 
     2821 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg 
     2881 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 
     2941 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 
     3001 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 
     3061 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 
     3121 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg 
     3181 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 
     3241 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 
     3301 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 
     3361 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 
     3421 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 
     3481 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca 
     3541 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 
     3601 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 
     3661 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 
     3721 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 
     3781 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 
     3841 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 
     3901 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 
     3961 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 
     4021 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 
     4081 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 
     4141 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 
     4201 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 
     4261 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 
     4321 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 
     4381 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 
     4441 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa 
     4501 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 
     4561 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 
     4621 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 
     4681 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 
     4741 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 
     4801 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 
     4861 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 
     4921 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 
     4981 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag 
     5041 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa 
     5101 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga 
     5161 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc 
     5221 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca 
     5281 atttcacaca ggaaacagct atgaccatga ttacgccaga tttaattaag gccttaatta 
     5341 gg 
//
