LOCUS       pENN.AAV.hSyn.HI        6733 bp    DNA     circular     21-OCT-2017
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT                                   "{\\i Sma}I (1027)" "@N (@S)" 5 13.962 0 0 
COMMENT                                   -10 4.17106 0.924127 #223#) 
COMMENT                             #225=(CScratch 
COMMENT                                   (CWidget 4 (1 #0# 4) 1 2 0 0 #142# 4063800 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 2.66748 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #226=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #123# 439072852 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Sma}I (3871)" "@N (@S)" 5 -12.0699 
COMMENT                                   -6.96932 0 -10 4.17106 0.924127 #225#) 
COMMENT                             #227=(CScratch 
COMMENT                                   (CWidget 5 (1 #0# 5) 1 2 0 0 #142# 48388468 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 2.65723 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #228=(CLabel (CWidget 0 (0 0) 1 2 0 0 #123# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Sma}I (3882)" "@N (@S)" 5 -13.0476 
COMMENT                                   -5.22699 0 -10 4.22101 0.924127 #227#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #229=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -392 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #230=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -597 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 100 
COMMENT       (CStringList) 0 0 (CObList)))
FEATURES             Location/Qualifiers
     rep_origin      complement(4089..4544)
                     /vntifkey="33"
                     /label=f1\ori
     insertion_seq   3745..3783
                     /vntifkey="14"
                     /label=AAV2\seq\upstream\of\3'\ITR
     insertion_seq   130..168
                     /vntifkey="14"
                     /label=AAV2\seq\upstream\of\3'\ITR
     polyA_signal    3479..3717
                     /vntifkey="25"
                     /label=SV40\polyA
     misc_marker     4675..5532
                     /vntifkey="22"
                     /label=AP(R)
     rep_origin      5706..6294
                     /vntifkey="33"
                     /label=Origin\of\replication
     repeat_region   complement(3783..3912)
                     /vntifkey="34"
                     /label=3'\ITR
     repeat_region   1..130
                     /vntifkey="34"
                     /label=5'\ITR
     intron          737..1008
                     /vntifkey="15"
                     /label=hGHintron1\(AF264722.1)
     CDS             1044..1760
                     /vntifkey="4"
                     /label=GFP
     CDS             1822..2852
                     /vntifkey="4"
                     /label=Cre
     misc_marker     2874..3461
                     /vntifkey="22"
                     /label=WPRE
     promoter        213..678
                     /vntifkey="29"
                     /label=hSynapsin
BASE COUNT     1549 a      1818 c      1796 g      1570 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 
      181 aggaagatcc tctagaacta tagctagcat gcctgcagag ggccctgcgt atgagtgcaa 
      241 gtgggtttta ggaccaggat gaggcggggt gggggtgcct acctgacgac cgaccccgac 
      301 ccactggaca agcacccaac ccccattccc caaattgcgc atcccctatc agagaggggg 
      361 aggggaaaca ggatgcggcg aggcgcgtgc gcactgccag cttcagcacc gcggacagtg 
      421 ccttcgcccc cgcctggcgg cgcgcgccac cgccgcctca gcactgaagg cgcgctgacg 
      481 tcactcgccg gtcccccgca aactcccctt cccggccacc ttggtcgcgt ccgcgccgcc 
      541 gccggcccag ccggaccgca ccacgcgagg cgcgagatag gggggcacgg gcgcgaccat 
      601 ctgcgctgcg gcgccggcga ctcagcgctg cctcagtctg cggtgggcag cggaggagtc 
      661 gtgtcgtgcc tgagagcgca gtcgaattcg cgtggtacct ctagagtcga cgggaccgat 
      721 ccagcctccg taccggttcg aacaggtaag cgcccctaaa atccctttgg gcacaatgtg 
      781 tcctgagggg agaggcagcg acctgtagat gggacggggg cactaaccct caggtttggg 
      841 gcttctgaat gtgagtatcg ccatgtaagc ccagtatttg gccaatctca gaaagctcct 
      901 ggtccctgga gggatggaga gagaaaaaca aacagctcct ggagcaggga gagtgctggc 
      961 ctcttgctct ccggctccct ctgttgccct ctggtttctc cccaggttcg aatctagagg 
     1021 atccccgggt accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg 
     1081 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg 
     1141 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca 
     1201 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca 
     1261 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct 
     1321 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg 
     1381 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg 
     1441 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata 
     1501 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg 
     1561 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc 
     1621 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca 
     1681 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg 
     1741 gcatggacga gctgtacaag tccggagagc aaaagctgat ttctgaggag gatctgggag 
     1801 gacccaagaa gaagaggaag gtgtccaatt tactgaccgt acaccaaaat ttgcctgcat 
     1861 taccggtcga tgcaacgagt gatgaggttc gcaagaacct gatggacatg ttcagggatc 
     1921 gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc cgtttgccgg tcgtgggcgg 
     1981 catggtgcaa gttgaataac cggaaatggt ttcccgcaga acctgaagat gttcgcgatt 
     2041 atcttctata tcttcaggcg cgcggtctgg cagtaaaaac tatccagcaa catttgggcc 
     2101 agctaaacat gcttcatcgt cggtccgggc tgccacgacc aagtgacagc aatgctgttt 
     2161 cactggttat gcggcggatc cgaaaagaaa acgttgatgc cggtgaacgt gcaaaacagg 
     2221 ctctagcgtt cgaacgcact gatttcgacc aggttcgttc actcatggaa aatagcgatc 
     2281 gctgccagga tatacgtaat ctggcatttc tggggattgc ttataacacc ctgttacgta 
     2341 tagccgaaat tgccaggatc agggttaaag atatctcacg tactgacggt gggagaatgt 
     2401 taatccatat tggcagaacg aaaacgctgg ttagcaccgc aggtgtagag aaggcactta 
     2461 gcctgggggt aactaaactg gtcgagcgat ggatttccgt ctctggtgta gctgatgatc 
     2521 cgaataacta cctgttttgc cgggtcagaa aaaatggtgt tgccgcgcca tctgccacca 
     2581 gccagctatc aactcgcgcc ctggaaggga tttttgaagc aactcatcga ttgatttacg 
     2641 gcgctaagga tgactctggt cagagatacc tggcctggtc tggacacagt gcccgtgtcg 
     2701 gagccgcgcg agatatggcc cgcgctggag tttcaatacc ggagatcatg caagctggtg 
     2761 gctggaccaa tgtaaatatt gtcatgaact atatccgtaa cctggatagt gaaacagggg 
     2821 caatggtgcg cctgctggaa gatggcgatt agctcgatca agcttatcga taatcaacct 
     2881 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 
     2941 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 
     3001 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 
     3061 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 
     3121 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 
     3181 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 
     3241 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt 
     3301 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 
     3361 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc 
     3421 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgatcgagg 
     3481 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg 
     3541 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt 
     3601 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag 
     3661 ggggagatgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat 
     3721 aaggatcttc ctagagcatg gctacgtaga taagtagcat ggcgggttaa tcattaacta 
     3781 caaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga 
     3841 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga 
     3901 gcgagcgcgc agccttaatt aacctaattc actggccgtc gttttacaac gtcgtgactg 
     3961 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg 
     4021 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg 
     4081 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 
     4141 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt 
     4201 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 
     4261 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg 
     4321 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 
     4381 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 
     4441 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 
     4501 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg 
     4561 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 
     4621 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 
     4681 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 
     4741 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 
     4801 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 
     4861 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 
     4921 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 
     4981 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 
     5041 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 
     5101 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 
     5161 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 
     5221 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 
     5281 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 
     5341 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 
     5401 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 
     5461 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 
     5521 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 
     5581 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 
     5641 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 
     5701 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 
     5761 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 
     5821 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 
     5881 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 
     5941 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 
     6001 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 
     6061 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 
     6121 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 
     6181 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 
     6241 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 
     6301 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 
     6361 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 
     6421 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 
     6481 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 
     6541 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 
     6601 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 
     6661 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccag atttaattaa 
     6721 ggccttaatt agg 
//
