LOCUS       pENN.AAV.CB7.CI.        6548 bp    DNA     circular     22-OCT-2017
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11994329|
COMMENT     VNTDBDATE|-11994329|
COMMENT     LSOWNER|
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COMMENT                                   (CWidget 2 (1 #3# 2) 1 2 0 0 #146# 996 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 5.00667 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #226=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #127# 1993691104 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Bsg}I (1323)" "@N (@S)" 5 13.8487 0 0 
COMMENT                                   -10 3.87134 0.924127 #225#) 
COMMENT                             #227=(CScratch 
COMMENT                                   (CWidget 3 (1 #3# 3) 1 2 0 0 #146# 939 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 4.25653 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #228=(CLabel (CWidget 0 (0 0) 1 2 0 0 #127# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Bsg}I (2106)" "@N (@S)" 5 12.8734 
COMMENT                                   -5.22699 0 -10 3.87134 0.924127 #227#) 
COMMENT                             #229=(CScratch 
COMMENT                                   (CWidget 4 (1 #3# 4) 1 2 0 0 #146# 37461552 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 4.1636 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #230=(CLabel (CWidget 0 (0 0) 1 2 0 0 #127# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Bsg}I (2203)" "@N (@S)" 5 12.4959 
COMMENT                                   -6.96932 0 -10 3.92129 0.924127 #229#) 
COMMENT                             #231=(CScratch 
COMMENT                                   (CWidget 5 (1 #3# 5) 1 2 0 0 #146# 544042866 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 3.8532 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #232=(CLabel (CWidget 0 (0 0) 1 2 0 0 #127# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Bsg}I (2527)" "@N (@S)" 5 10.3511 
COMMENT                                   -10.454 0 -10 3.92129 0.924127 #231#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #233=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -598 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #234=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1295 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 25552360 
COMMENT       (CStringList) 12 12 
COMMENT       (CObList 
COMMENT        #235=(CSampFrag "pENN.AAV.CB7.CI.EGFP.WPRE.RBG (P1963)-Q" 2883 3697 
COMMENT              "SmaI" 32 "SmaI" (LOGPEN 0 0 0) 1.25928e-316 3.07874e-289) 
COMMENT        #236=(CSampFrag "pENN.AAV.CB7.CI.EGFP.WPRE.RBG (P1963)-Q" 1647 186 
COMMENT              "XbaI" 1833 "XbaI" (LOGPEN 0 0 0) 1.23543e-316 1.02894e-314) 
COMMENT        #237=(CSampFrag "pENN.AAV.CB7.CI.EGFP.WPRE.RBG (P1963)-Q" 962 2724 
COMMENT              "XbaI" 3686 "SmaI" (LOGPEN 0 0 0) 2.19265e-316 2.5779e-312) 
COMMENT        #238=(CSampFrag "pENN.AAV.CB7.CI.EGFP.WPRE.RBG (P1963)-Q" 891 1833 
COMMENT              "XbaI" 2724 "XbaI" (LOGPEN 0 0 0) 1.2279e-316 1.02894e-314) 
COMMENT        #239=(CSampFrag "pENN.AAV.CB7.CI.EGFP.WPRE.RBG (P1963)-Q" 143 43 "SmaI" 
COMMENT              186 "XbaI" (LOGPEN 0 0 0) 1.24399e-316 2.58411e-302) 
COMMENT        #240=(CSampFrag "pENN.AAV.CB7.CI.EGFP.WPRE.RBG (P1963)-Q" 11 3686 "SmaI" 
COMMENT              3697 "SmaI" (LOGPEN 0 0 0) 1.05378e-316 1.66942e-307) 
COMMENT        #241=(CSampFrag "pENN.AAV.CB7.CI.EGFP.WPRE.RBG (P1963)-Q" 11 32 "SmaI" 
COMMENT              43 "SmaI" (LOGPEN 0 0 0) 1.05783e-316 100))))
FEATURES             Location/Qualifiers
     CDS             1977..2696
                     /vntifkey="4"
                     /label=eGFP
     repeat_region   198..579
                     /vntifkey="34"
                     /label=CMV\IE\promoter
     rep_origin      complement(3904..4359)
                     /vntifkey="33"
                     /label=f1\ori
     misc_marker     4490..5347
                     /vntifkey="22"
                     /label=AP(R)
     rep_origin      5521..6109
                     /vntifkey="33"
                     /label=Origin\of\replication
     repeat_region   complement(3598..3727)
                     /vntifkey="34"
                     /label=3'\ITR
     repeat_region   1..130
                     /vntifkey="34"
                     /label=5'\ITR
     polyA_signal    3383..3509
                     /vntifkey="25"
                     /label=Rabbit\globin\poly\A
     TATA_signal     836..839
                     /vntifkey="41"
                     /label=TATA
     intron          956..1928
                     /vntifkey="15"
                     /label=chicken\beta-actin\intron
     promoter        582..863
                     /vntifkey="29"
                     /label=CB\promoter
     misc_marker     2773..3360
                     /vntifkey="22"
                     /label=WPRE
BASE COUNT     1338 a      1821 c      1866 g      1523 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag ggtaatgggg 
      181 atcctctaga actatagcta gtcgacattg attattgact agttattaat agtaatcaat 
      241 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 
      301 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 
      361 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 
      421 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 
      481 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 
      541 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac 
      601 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat 
      661 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg 
      721 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca 
      781 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa 
      841 aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc 
      901 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag 
      961 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt 
     1021 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg 
     1081 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc 
     1141 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt 
     1201 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa 
     1261 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt 
     1321 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg 
     1381 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca 
     1441 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg 
     1501 cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt 
     1561 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa 
     1621 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg 
     1681 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc 
     1741 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc 
     1801 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc 
     1861 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt 
     1921 tggcaaagaa ttacttaata cgactcacta taggctagta atacgactca ctatagatgg 
     1981 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg 
     2041 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca 
     2101 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg 
     2161 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc 
     2221 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca 
     2281 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga 
     2341 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc 
     2401 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca 
     2461 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc 
     2521 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc 
     2581 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc 
     2641 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg 
     2701 gccctagcgt ttaaacgggc cctctagact cgaggacggg gtgaactacg cctgaatcaa 
     2761 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa 
     2821 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat 
     2881 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta 
     2941 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc 
     3001 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt 
     3061 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg 
     3121 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc 
     3181 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc 
     3241 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct 
     3301 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca 
     3361 tcgataccgt cgatgaggat ccgatctttt tccctctgcc aaaaattatg gggacatcat 
     3421 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt 
     3481 gtgttggaat tttttgtgtc tctcactcgg aagcaattcg ttgatctgaa tttcgaccac 
     3541 ccataatacc cattaccctg gtagataagt agcatggcgg gttaatcatt aactacaagg 
     3601 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg 
     3661 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag 
     3721 cgcgcagcct taattaacct aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa 
     3781 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta 
     3841 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat 
     3901 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 
     3961 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 
     4021 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 
     4081 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 
     4141 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 
     4201 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 
     4261 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 
     4321 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa 
     4381 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 
     4441 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca 
     4501 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca 
     4561 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta 
     4621 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt 
     4681 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc 
     4741 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc 
     4801 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc 
     4861 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa 
     4921 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga 
     4981 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat 
     5041 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca 
     5101 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc 
     5161 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat 
     5221 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag 
     5281 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa 
     5341 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca 
     5401 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 
     5461 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 
     5521 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 
     5581 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 
     5641 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 
     5701 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 
     5761 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 
     5821 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 
     5881 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 
     5941 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 
     6001 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 
     6061 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 
     6121 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 
     6181 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 
     6241 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat 
     6301 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 
     6361 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 
     6421 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 
     6481 ataacaattt cacacaggaa acagctatga ccatgattac gccagattta attaaggcct 
     6541 taattagg 
//
