LOCUS       pENN.AAV.cTNT.PI        5572 bp    DNA     circular     22-OCT-2017
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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FEATURES             Location/Qualifiers
     rep_origin      complement(2928..3383)
                     /vntifkey="33"
                     /label=f1\ori
     misc_marker     3514..4371
                     /vntifkey="22"
                     /label=AP(R)
     rep_origin      4545..5133
                     /vntifkey="33"
                     /label=Origin\of\replication
     repeat_region   complement(2622..2751)
                     /vntifkey="34"
                     /label=3'\ITR
     repeat_region   1..130
                     /vntifkey="34"
                     /label=5'\ITR
     polyA_signal    2407..2533
                     /vntifkey="25"
                     /label=Rabbit\globin\poly\A
     intron          772..904
                     /vntifkey="15"
                     /label=Intron_1
                     /note="chimeric intron"
     CDS             997..1712
                     /vntifkey="4"
                     /label=eGFP
     promoter        214..655
                     /vntifkey="29"
                     /label=CTNT
     misc_marker     1798..2385
                     /vntifkey="22"
                     /label=WPRE
BASE COUNT     1254 a      1540 c      1444 g      1334 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag ggtaatgggg 
      181 atcctctaga actatagcta gaattcgccc ttacgggccc cccctcgagg tcgggataaa 
      241 agcagtctgg gctttcacat gacagcatct ggggctgcgg cagagggtcg ggtccgaagc 
      301 gctgccttat cagcgtcccc agccctggga ggtgacagct ggctggcttg tgtcagcccc 
      361 tcgggcactc acgtatctcc gtccgacggg tttaaaatag caaaactctg aggccacaca 
      421 atagcttggg cttatatggg ctcctgtggg ggaaggggga gcacggaggg ggccggggcc 
      481 gctgctgcca aaatagcagc tcacaagtgt tgcattcctc tctgggcgcc gggcacattc 
      541 ctgctggctc tgcccgcccc ggggtgggcg ccggggggac cttaaagcct ctgcccccca 
      601 aggagccctt cccagacagc cgccggcacc caccgctccg tgggacgatc cccgaagctc 
      661 tagagcttta ttgcggtagt ttatcacagt taaattgcta acgcagtcag tgcttctgac 
      721 acaacagtct cgaacttaag ctgcagaagt tggtcgtgag gcactgggca ggtaagtatc 
      781 aaggttacaa gacaggttta aggagaccaa tagaaactgg gcttgtcgag acagagaaga 
      841 ctcttgcgtt tctgataggc acctattggt cttactgaca tccactttgc ctttctctcc 
      901 acaggtgtcc actcccagtt caattacagc tcttaaggct agagtactta atacgactca 
      961 ctataggcta gcctcgagaa ttcacgcggg ccgccatggt gagcaagggc gaggagctgt 
     1021 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca 
     1081 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 
     1141 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg 
     1201 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca 
     1261 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 
     1321 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 
     1381 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc 
     1441 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc 
     1501 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 
     1561 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga 
     1621 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 
     1681 ggatcactct cggcatggac gagctgtaca agtaataagc tcgcgtggta cctctagagt 
     1741 cgacccgggc ggcctcgagg acggggtgaa ctacgcctga atcaagctta tcgataatca 
     1801 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 
     1861 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 
     1921 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 
     1981 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 
     2041 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 
     2101 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 
     2161 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg 
     2221 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 
     2281 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 
     2341 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgacgtga 
     2401 ggatccgatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat 
     2461 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg 
     2521 tgtctctcac tcggaagcaa ttcgttgatc tgaatttcga ccacccataa tacccattac 
     2581 cctggtagat aagtagcatg gcgggttaat cattaactac aaggaacccc tagtgatgga 
     2641 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc 
     2701 ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gccttaatta 
     2761 acctaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca 
     2821 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 
     2881 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag 
     2941 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 
     3001 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 
     3061 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 
     3121 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 
     3181 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 
     3241 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 
     3301 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa 
     3361 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct 
     3421 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 
     3481 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 
     3541 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 
     3601 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 
     3661 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 
     3721 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc 
     3781 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 
     3841 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 
     3901 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 
     3961 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 
     4021 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc 
     4081 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 
     4141 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 
     4201 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 
     4261 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 
     4321 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 
     4381 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 
     4441 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 
     4501 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 
     4561 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 
     4621 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 
     4681 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 
     4741 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 
     4801 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 
     4861 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 
     4921 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 
     4981 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 
     5041 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 
     5101 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 
     5161 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 
     5221 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 
     5281 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc 
     5341 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg 
     5401 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta 
     5461 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca 
     5521 ggaaacagct atgaccatga ttacgccaga tttaattaag gccttaatta gg 
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