LOCUS       pENN.AAV.CB7.CI.        6502 bp    DNA     circular     22-OCT-2017
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11994635|
COMMENT     VNTDBDATE|-11994635|
COMMENT     LSOWNER|
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COMMENT                                      #109=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1993 " 1) 
COMMENT                                      #110=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 3576 " 1))))) "MscI: 3 sites") 
COMMENT                            3 3 "TGGCCA" "ACCGGT") 
COMMENT                      #111=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 67747888 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #112=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #113=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 32 " 1) 
COMMENT                                      #114=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 43 " 1) 
COMMENT                                      #115=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 3640 " 1) 
COMMENT                                      #116=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 3651 " 1))))) "SmaI: 4 sites") 
COMMENT                            3 3 "CCCGGG" "GGGCCC") 
COMMENT                      #117=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 67015280 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #118=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #119=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 560 " 1))))) "SnaBI: 1 site") 
COMMENT                            3 3 "TACGTA" "ATGCAT") 
COMMENT                      #120=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 67015632 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #121=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #122=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 186 " 1) 
COMMENT                                      #123=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1833 " 1))))) "XbaI: 2 sites") 
COMMENT                            1 5 "TCTAGA" "AGATCT"))) 
COMMENT                    "Restriction/Methylation Map") 
COMMENT              #124=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 15 (12 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #125=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 0 (13 0 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #126=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 1 (14 1 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #127=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 42014856 (4 #25# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #128=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #129=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  493 - 840" 1))) 
COMMENT                                            "493(d1) (116 codons)") 
COMMENT                                      #130=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 42015024 (4 #28# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #131=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #132=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  2815 - 3330" 
COMMENT                                                    1))) "2815(d1) (172 codons)") 
COMMENT                                      #133=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 42015136 (4 #30# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #134=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #135=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  4444 - 5304" 
COMMENT                                                    1))) "4444(d1) (287 codons)"))) 
COMMENT                                    "Reading Frame 1 (3 ORFs)") 
COMMENT                              #136=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 2 (14 2 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #137=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 42015080 (4 #29# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #138=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #139=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  2828 - 3376" 
COMMENT                                                    1))) "2828(d2) (183 codons)"))) 
COMMENT                                    "Reading Frame 2 (1 ORF)") 
COMMENT                              #140=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 3 (14 3 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #141=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 42014912 (4 #26# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #142=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #143=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  1962 - 2672" 
COMMENT                                                    1))) "1962(d3) (237 codons)"))) 
COMMENT                                    "Reading Frame 3 (1 ORF)"))) 
COMMENT                            "Direct(Forward) strand") 
COMMENT                      #144=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 1 (13 1 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #145=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 1 (14 -1 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #146=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 42014968 (4 #27# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #147=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #148=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  944 - 1552" 
COMMENT                                                    1))) "944(c1) (203 codons)"))) 
COMMENT                                    "Reading Frame -1 (1 ORF)") 
COMMENT                              #149=(CFolderPar 
COMMENT                                    (CGroupPar (CParagraph 2 (0 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList)) "Reading Frame -2 (0 ORFs)") 
COMMENT                              #150=(CFolderPar 
COMMENT                                    (CGroupPar (CParagraph 3 (0 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList)) "Reading Frame -3 (0 ORFs)"))) 
COMMENT                            "Complementary(Reverse) strand"))) 
COMMENT                    "Open Reading Frames") 
COMMENT              #151=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 20 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #152=(CRefLinePar 
COMMENT                            (CLinePar (CParagraph 0 (0 0) 0 2 1 0 235) 
COMMENT                             " 2883: pENN.AAV.CB7.CI.mCherry.WP.RBG (p1969)-Q: SmaI(3651) - SmaI(32)" 
COMMENT                             1) 4 "" 3651 31) 
COMMENT                      #153=(CRefLinePar 
COMMENT                            (CLinePar (CParagraph 0 (0 0) 0 2 1 0 235) 
COMMENT                             " 1807: pENN.AAV.CB7.CI.mCherry.WP.RBG (p1969)-Q: XbaI(1833) - SmaI(3640)" 
COMMENT                             1) 4 "" 1833 3639) 
COMMENT                      #154=(CRefLinePar 
COMMENT                            (CLinePar (CParagraph 0 (0 0) 0 2 1 0 235) 
COMMENT                             " 1647: pENN.AAV.CB7.CI.mCherry.WP.RBG (p1969)-Q: XbaI(186) - XbaI(1833)" 
COMMENT                             1) 4 "" 186 1832) 
COMMENT                      #155=(CRefLinePar 
COMMENT                            (CLinePar (CParagraph 0 (0 0) 0 2 1 0 235) 
COMMENT                             " 143: pENN.AAV.CB7.CI.mCherry.WP.RBG (p1969)-Q: SmaI(43) - XbaI(186)" 
COMMENT                             1) 4 "" 43 185) 
COMMENT                      #156=(CRefLinePar 
COMMENT                            (CLinePar (CParagraph 0 (0 0) 0 2 1 0 235) 
COMMENT                             " 11: pENN.AAV.CB7.CI.mCherry.WP.RBG (p1969)-Q: SmaI(3640) - SmaI(3651)" 
COMMENT                             1) 4 "" 3640 3650) 
COMMENT                      #157=(CRefLinePar 
COMMENT                            (CLinePar (CParagraph 0 (0 0) 0 2 1 0 235) 
COMMENT                             " 11: pENN.AAV.CB7.CI.mCherry.WP.RBG (p1969)-Q: SmaI(32) - SmaI(43)" 
COMMENT                             1) 4 "" 32 42))) "Restriction Fragments")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #158=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #159=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #160=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #161=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #162=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 
COMMENT                1 65535 "@N (@H)" 0) 
COMMENT          #163=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #164=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #165=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #166=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #167=(CWidgetStyle "Molecule Label 2" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #168=(CWidgetStyle "Molecule Label 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #169=(CWidgetStyle "Shape GAP" 1 (LOGPEN 1 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #170=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #171=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 
COMMENT                (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #172=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #173=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 
COMMENT                (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #174=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) 
COMMENT          #175=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 
COMMENT                (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) 
COMMENT          #176=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 
COMMENT                (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) 
COMMENT          #177=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #178=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #179=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #180=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #181=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #182=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #183=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #184=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #185=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #186=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #187=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #188=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #189=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #190=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #191=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #192=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 65535 "@N" 0)) 0.164644 1.74233 0.164644 2.53336 
COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 58.5 58.5 0.1 
COMMENT         -34817) 1 1 0 1 1 
COMMENT        (mapper: 21.7601 -21.3753 40.0378 40.0378 0.01 10 -1 6502 6502 1 0 0) 
COMMENT        #193=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 0 100) 
COMMENT              (CObjectList 
COMMENT               #194=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -385 100) 
COMMENT                     (CObjectList 
COMMENT                      #195=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #175# 67711508 0) 
COMMENT                             (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 
COMMENT                             6.27515 6.27315 1 0.0214037) 0.0964809) 
COMMENT                      #196=(CLabel (CWidget 1001 (0 0) 1 2 0 0 #168# 460 100) 
COMMENT                            (LOGPEN 456 -823 4294966436) 457 
COMMENT                            (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 
COMMENT                            1.74233 0.833333 0 
COMMENT                            "pENN.AAV.CB7.CI.mCherry.WPRE.rBG (p1969)" "@N" 1 0 
COMMENT                            0.871165 0 -10 25.5758 1.47361 Nil) 
COMMENT                      #197=(CLabel (CWidget 1002 (0 0) 1 2 0 0 #167# 0 100) 
COMMENT                            (LOGPEN 1634300481 108 0) 0 
COMMENT                            (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 
COMMENT                            1.74233 0.555556 0 "6502 bp" "@L bp" 16 0 -0.871165 
COMMENT                            0 -10.8 2.59755 0.924127 Nil)) (CObjectList)) 
COMMENT               #198=(CGroupWidget (CWidget 13 (12 0) 1 2 0 0 Nil -525 100) 
COMMENT                     (CObjectList 
COMMENT                      #199=(CGroupWidget 
COMMENT                            (CWidget 0 (13 0 0) 1 2 0 0 Nil -667 100) 
COMMENT                            (CObjectList 
COMMENT                             #200=(CGroupWidget 
COMMENT                                   (CWidget 1 (14 1 0) 1 2 0 0 Nil -248 100) 
COMMENT                                   (CObjectList 
COMMENT                                    #201=(CORFArrow 
COMMENT                                          (CLine 
COMMENT                                           (CWidget 1 (4 #25# 0) 1 2 0 0 #176# 
COMMENT                                            960 100) (LOGPEN 0 6 16384) 8 
COMMENT                                           1.16464 5.46275 5.7985) 0.2 0.609808 
COMMENT                                          0.082322 3.41182 2.86186 0 0 0) 
COMMENT                                    #202=(CORFArrow 
COMMENT                                          (CLine 
COMMENT                                           (CWidget 1 (4 #28# 0) 1 2 0 0 #176# 
COMMENT                                            601 100) (LOGPEN 0 6 16384) 8 
COMMENT                                           1.16464 3.06037 3.55821) 0.2 0.609808 
COMMENT                                          0.082322 3.41182 2.86186 0 0 0) 
COMMENT                                    #203=(CORFArrow 
COMMENT                                          (CLine 
COMMENT                                           (CWidget 1 (4 #30# 0) 1 2 0 0 #176# 0 
COMMENT                                            100) (LOGPEN 0 6 16384) 8 1.16464 
COMMENT                                           1.15584 1.98654) 0.2 0.609808 
COMMENT                                          0.082322 3.41182 2.86186 0 0 0)) 
COMMENT                                   (CObjectList)) 
COMMENT                             #204=(CGroupWidget 
COMMENT                                   (CWidget 2 (14 2 0) 1 2 0 0 Nil -1295 100) 
COMMENT                                   (CObjectList 
COMMENT                                    #205=(CORFArrow 
COMMENT                                          (CLine 
COMMENT                                           (CWidget 2 (4 #29# 0) 1 2 0 0 #176# 
COMMENT                                            453 100) (LOGPEN 0 6 16384) 8 
COMMENT                                           1.32929 3.01599 3.54567) 0.2 0.609808 
COMMENT                                          0.082322 3.41182 2.86186 0 0 0)) 
COMMENT                                   (CObjectList)) 
COMMENT                             #206=(CGroupWidget 
COMMENT                                   (CWidget 3 (14 3 0) 1 2 0 0 Nil -1504 100) 
COMMENT                                   (CObjectList 
COMMENT                                    #207=(CORFArrow 
COMMENT                                          (CLine 
COMMENT                                           (CWidget 3 (4 #26# 0) 1 2 0 0 #176# 
COMMENT                                            1084 100) (LOGPEN 0 6 16384) 8 
COMMENT                                           1.49393 3.69522 4.3812) 0.2 0.609808 
COMMENT                                          0.082322 3.41182 2.86186 0 0 0)) 
COMMENT                                   (CObjectList))) (CObjectList)) 
COMMENT                      #208=(CGroupWidget 
COMMENT                            (CWidget 1 (13 1 0) 1 2 0 0 Nil -458 100) 
COMMENT                            (CObjectList 
COMMENT                             #209=(CGroupWidget 
COMMENT                                   (CWidget 1 (14 -1 0) 1 2 0 0 Nil -388 100) 
COMMENT                                   (CObjectList 
COMMENT                                    #210=(CORFArrow 
COMMENT                                          (CLine 
COMMENT                                           (CWidget 1 (4 #27# 0) 1 2 0 0 #176# 
COMMENT                                            1139 100) (LOGPEN 0 6 16384) 8 
COMMENT                                           0.835356 4.7758 5.36337) 0.2 0.609808 
COMMENT                                          0.082322 3.41182 2.86186 1 0 0)) 
COMMENT                                   (CObjectList))) (CObjectList))) (CObjectList)) 
COMMENT               #211=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -597 100) 
COMMENT                     (CObjectList 
COMMENT                      #212=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -876 100) 
COMMENT                            (CObjectList 
COMMENT                             #213=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #14# 0) 1 2 0 0 #171# 0 100) 
COMMENT                                    (LOGPEN 0 0 3355545) 1 
COMMENT                                    (LOGBRUSH 0 6724095 0) 1 3.69522 4.3812 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #214=(CLabel (CWidget 0 (0 0) 1 2 0 0 #164# 1 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "mCherry( monomeric red fluorescent protein gene)" 
COMMENT                                   "@N" 1 23.9637 -10.454 0 -10 19.8063 1.07399 
COMMENT                                   #213#)) (CObjectList)) 
COMMENT                      #215=(CGroupWidget 
COMMENT                            (CWidget 15 (7 15 0) 1 2 0 0 Nil -849 100) 
COMMENT                            (CObjectList 
COMMENT                             #216=(CLine 
COMMENT                                   (CWidget 0 (3 #23# 0) 1 2 0 0 #180# 1330 100) 
COMMENT                                   (LOGPEN 0 0 16711680) 1 0.670712 4.41303 
COMMENT                                   5.35179) 
COMMENT                             #217=(CLabel (CWidget 0 (0 0) 1 2 0 0 #164# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "chicken beta-actin intron" "@N" 1 21.6647 
COMMENT                                   3.48466 0 -10 9.69084 1.07399 #216#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #218=(CGroupWidget 
COMMENT                            (CWidget 22 (7 22 0) 1 2 0 0 Nil -1644 100) 
COMMENT                            (CObjectList 
COMMENT                             #219=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #13# 0) 1 2 0 0 #172# 
COMMENT                                     1667321128 100) (LOGPEN 0 0 0) 1 
COMMENT                                    (LOGBRUSH 0 128 0) 1 3.07581 3.64312 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #220=(CLabel (CWidget 0 (0 0) 1 2 0 0 #164# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "WPRE" "@N" 1 
COMMENT                                   5.76421 -17.4233 0 -10 2.54759 1.07399 #219#) 
COMMENT                             #221=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #17# 0) 1 2 0 0 #172# 613 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 1 
COMMENT                                    1.15874 1.98654 1 0.082322) 0.8 1.8 0) 
COMMENT                             #222=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #164# 12632256 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "AP{\\super r}" 
COMMENT                                   "@N" 1 -17.5693 0 0 -10 1.52356 1.07399 #221#)) 
COMMENT                            (CObjectList)) 
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COMMENT       (CStringList) 12 12 
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COMMENT        #276=(CSampFrag "pENN.AAV.CB7.CI.mCherry.WP.RBG (p1969)-Q" 2883 3651 
COMMENT              "SmaI" 32 "SmaI" (LOGPEN 0 0 0) 1.97626e-323 2.58883e-312) 
COMMENT        #277=(CSampFrag "pENN.AAV.CB7.CI.mCherry.WP.RBG (p1969)-Q" 1807 1833 
COMMENT              "XbaI" 3640 "SmaI" (LOGPEN 0 0 0) 2.00305e-316 2.5779e-312) 
COMMENT        #278=(CSampFrag "pENN.AAV.CB7.CI.mCherry.WP.RBG (p1969)-Q" 1647 186 
COMMENT              "XbaI" 1833 "XbaI" (LOGPEN 0 0 0) 1.6976e-313 6.20801e-295) 
COMMENT        #279=(CSampFrag "pENN.AAV.CB7.CI.mCherry.WP.RBG (p1969)-Q" 143 43 "SmaI" 
COMMENT              186 "XbaI" (LOGPEN 0 0 0) 3.87206e+228 6.4166e-296) 
COMMENT        #280=(CSampFrag "pENN.AAV.CB7.CI.mCherry.WP.RBG (p1969)-Q" 11 3640 
COMMENT              "SmaI" 3651 "SmaI" (LOGPEN 0 0 0) 1.51489e-301 132396) 
COMMENT        #281=(CSampFrag "pENN.AAV.CB7.CI.mCherry.WP.RBG (p1969)-Q" 11 32 "SmaI" 
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FEATURES             Location/Qualifiers
     misc_marker     2727..3314
                     /vntifkey="22"
                     /label=WPRE
     CDS             1962..2672
                     /vntifkey="4"
                     /label=mCherry(\monomeric\red\fluorescent\protein\gene)
     repeat_region   198..579
                     /vntifkey="34"
                     /label=CMV\IE\enhancer
     rep_origin      complement(3858..4313)
                     /vntifkey="33"
                     /label=f1\ori
     misc_marker     4444..5301
                     /vntifkey="22"
                     /label=AP(R)
     rep_origin      5475..6063
                     /vntifkey="33"
                     /label=Origin\of\replication
     repeat_region   complement(3552..3681)
                     /vntifkey="34"
                     /label=3'\ITR
     repeat_region   1..130
                     /vntifkey="34"
                     /label=5'\ITR
     polyA_signal    3337..3463
                     /vntifkey="25"
                     /label=Rabbit\globin\poly\A
     TATA_signal     836..839
                     /vntifkey="41"
                     /label=TATA
     intron          956..1928
                     /vntifkey="15"
                     /label=chicken\beta-actin\intron
     promoter        582..863
                     /vntifkey="29"
                     /label=CB\promoter
BASE COUNT     1321 a      1792 c      1877 g      1512 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag ggtaatgggg 
      181 atcctctaga actatagcta gtcgacattg attattgact agttattaat agtaatcaat 
      241 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 
      301 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 
      361 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 
      421 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 
      481 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 
      541 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac 
      601 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat 
      661 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg 
      721 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca 
      781 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa 
      841 aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc 
      901 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag 
      961 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt 
     1021 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg 
     1081 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc 
     1141 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt 
     1201 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa 
     1261 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt 
     1321 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg 
     1381 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca 
     1441 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg 
     1501 cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt 
     1561 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa 
     1621 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg 
     1681 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc 
     1741 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc 
     1801 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc 
     1861 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt 
     1921 tggcaaagaa ttcacgctag atttaagaag gagatataca tatggtgagc aagggcgagg 
     1981 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg 
     2041 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc 
     2101 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt 
     2161 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact 
     2221 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg 
     2281 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg 
     2341 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg 
     2401 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca 
     2461 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca 
     2521 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca 
     2581 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact 
     2641 ccaccggcgg catggacgag ctgtacaagt aagcatgcaa gcttcgagga cggggtgaac 
     2701 tacgcctgaa tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg 
     2761 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 
     2821 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 
     2881 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 
     2941 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 
     3001 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 
     3061 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa 
     3121 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 
     3181 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 
     3241 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg 
     3301 gccgcctccc cgcatcgata ccgtcgatga ggatccgatc tttttccctc tgccaaaaat 
     3361 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt 
     3421 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaagcaa ttcgttgatc 
     3481 tgaatttcga ccacccataa tacccattac cctggtagat aagtagcatg gcgggttaat 
     3541 cattaactac aaggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc 
     3601 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc 
     3661 agtgagcgag cgagcgcgca gccttaatta acctaattca ctggccgtcg ttttacaacg 
     3721 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt 
     3781 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag 
     3841 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 
     3901 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 
     3961 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc 
     4021 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga 
     4081 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 
     4141 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt 
     4201 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct 
     4261 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc 
     4321 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat 
     4381 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 
     4441 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt 
     4501 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt 
     4561 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc 
     4621 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta 
     4681 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac 
     4741 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa 
     4801 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg 
     4861 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc 
     4921 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg 
     4981 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta 
     5041 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg 
     5101 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg 
     5161 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc 
     5221 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt 
     5281 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt 
     5341 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 
     5401 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 
     5461 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 
     5521 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 
     5581 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag 
     5641 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 
     5701 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 
     5761 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 
     5821 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 
     5881 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 
     5941 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 
     6001 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 
     6061 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 
     6121 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga 
     6181 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg 
     6241 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 
     6301 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt 
     6361 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt 
     6421 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaga 
     6481 tttaattaag gccttaatta gg 
//
