LOCUS       pAAV.CMV.HI.GFP-        6931 bp    DNA     circular     22-OCT-2017
SOURCE      
  ORGANISM  
COMMENT     GenBank: AF264722.1 for intron
            
            GenBank: J03071.1 human growth horomone intron-1
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDATE|-11994648|
COMMENT     VNTDBDATE|-11994648|
COMMENT     LSOWNER|
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COMMENT              3826 "SmaI" 32 "SmaI" (LOGPEN 0 0 0) 2.32835e-301 2.98177e-306) 
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FEATURES             Location/Qualifiers
     misc_feature    5904..6492
                     /vntifkey="21"
                     /label=Origin
     CDS             4873..5730
                     /vntifkey="4"
                     /label=Amp-R
     repeat_region   complement(3727..3856)
                     /vntifkey="34"
                     /label=ITR
     misc_feature    145..714
                     /vntifkey="21"
                     /label=CMV\promoter\+Enhancer
     intron          807..1078
                     /vntifkey="15"
                     /label=hGHintron1\(AF264722.1)
     CDS             1114..1830
                     /vntifkey="4"
                     /label=GFP
     CDS             1892..2922
                     /vntifkey="4"
                     /label=Cre
     polyA_signal    3564..3623
                     /vntifkey="25"
                     /label=SV40\polyA
     LTR             1..130
                     /vntifkey="19"
                     /label=5'ITR
     misc_marker     2945..3532
                     /vntifkey="22"
                     /label=WPRE
BASE COUNT     1608 a      1846 c      1794 g      1683 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct gcggccgcac gcgtactagt tattaatagt aatcaattac ggggtcatta 
      181 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc 
      241 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg 
      301 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg 
      361 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa 
      421 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac 
      481 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 
      541 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 
      601 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 
      661 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta 
      721 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac 
      781 cgggaccgat ccagcctccg taccggttcg aacaggtaag cgcccctaaa atccctttgg 
      841 gcacaatgtg tcctgagggg agaggcagcg acctgtagat gggacggggg cactaaccct 
      901 caggtttggg gcttctgaat gtgagtatcg ccatgtaagc ccagtatttg gccaatctca 
      961 gaaagctcct ggtccctgga gggatggaga gagaaaaaca aacagctcct ggagcaggga 
     1021 gagtgctggc ctcttgctct ccggctccct ctgttgccct ctggtttctc cccaggttcg 
     1081 aatctagagg atccccgggt accggtcgcc accatggtga gcaagggcga ggagctgttc 
     1141 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 
     1201 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 
     1261 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg 
     1321 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 
     1381 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 
     1441 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 
     1501 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 
     1561 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 
     1621 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 
     1681 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 
     1741 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 
     1801 atcactctcg gcatggacga gctgtacaag tccggagagc aaaagctgat ttctgaggag 
     1861 gatctgggag gacccaagaa gaagaggaag gtgtccaatt tactgaccgt acaccaaaat 
     1921 ttgcctgcat taccggtcga tgcaacgagt gatgaggttc gcaagaacct gatggacatg 
     1981 ttcagggatc gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc cgtttgccgg 
     2041 tcgtgggcgg catggtgcaa gttgaataac cggaaatggt ttcccgcaga acctgaagat 
     2101 gttcgcgatt atcttctata tcttcaggcg cgcggtctgg cagtaaaaac tatccagcaa 
     2161 catttgggcc agctaaacat gcttcatcgt cggtccgggc tgccacgacc aagtgacagc 
     2221 aatgctgttt cactggttat gcggcggatc cgaaaagaaa acgttgatgc cggtgaacgt 
     2281 gcaaaacagg ctctagcgtt cgaacgcact gatttcgacc aggttcgttc actcatggaa 
     2341 aatagcgatc gctgccagga tatacgtaat ctggcatttc tggggattgc ttataacacc 
     2401 ctgttacgta tagccgaaat tgccaggatc agggttaaag atatctcacg tactgacggt 
     2461 gggagaatgt taatccatat tggcagaacg aaaacgctgg ttagcaccgc aggtgtagag 
     2521 aaggcactta gcctgggggt aactaaactg gtcgagcgat ggatttccgt ctctggtgta 
     2581 gctgatgatc cgaataacta cctgttttgc cgggtcagaa aaaatggtgt tgccgcgcca 
     2641 tctgccacca gccagctatc aactcgcgcc ctggaaggga tttttgaagc aactcatcga 
     2701 ttgatttacg gcgctaagga tgactctggt cagagatacc tggcctggtc tggacacagt 
     2761 gcccgtgtcg gagccgcgcg agatatggcc cgcgctggag tttcaatacc ggagatcatg 
     2821 caagctggtg gctggaccaa tgtaaatatt gtcatgaact atatccgtaa cctggatagt 
     2881 gaaacagggg caatggtgcg cctgctggaa gatggcgatt agctcgaatc aagcttatcg 
     2941 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 
     3001 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 
     3061 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 
     3121 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 
     3181 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 
     3241 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 
     3301 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc 
     3361 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 
     3421 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 
     3481 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc 
     3541 gtcgatcgag aaatcgatcg cccaacttgt ttattgcagc ttataatggt tacaaataaa 
     3601 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 
     3661 tgtccaaact catcaatgta tcttatcatg tctggatctc cggacacgtg cggaccgagc 
     3721 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca 
     3781 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga 
     3841 gcgagcgagc gcgcagcctt aattaaccta attcactggc cgtcgtttta caacgtcgtg 
     3901 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcagctgcc tgcaggggcg 
     3961 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag 
     4021 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 
     4081 agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc 
     4141 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 
     4201 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca 
     4261 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 
     4321 tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc gggctattct 
     4381 tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa 
     4441 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc 
     4501 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct 
     4561 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc 
     4621 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag 
     4681 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg 
     4741 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 
     4801 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 
     4861 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca 
     4921 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat 
     4981 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 
     5041 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 
     5101 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct 
     5161 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 
     5221 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 
     5281 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 
     5341 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 
     5401 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta 
     5461 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 
     5521 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 
     5581 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 
     5641 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 
     5701 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata 
     5761 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 
     5821 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 
     5881 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 
     5941 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 
     6001 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 
     6061 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 
     6121 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 
     6181 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 
     6241 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 
     6301 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 
     6361 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 
     6421 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 
     6481 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 
     6541 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 
     6601 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc 
     6661 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 
     6721 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt 
     6781 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt 
     6841 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat 
     6901 tacgccagat ttaattaagg ccttaattag g 
//
