LOCUS       pAAV.EF1a.eGFP.W        6308 bp    DNA     circular     22-OCT-2017
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|583597815|
COMMENT     VNTDBDATE|-11995011|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV.EF1a.eGFP.WPRE.RBG (p2213)|
COMMENT     VNTAUTHORNAME|Demo User|
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FEATURES             Location/Qualifiers
     CDS             1751..2466
                     /vntifkey="4"
                     /label=eGFP
     intron          1518..1650
                     /vntifkey="15"
                     /label=Intron_1
                     /note="chimeric intron"
     polyA_signal    3143..3269
                     /vntifkey="25"
                     /label=Rabbit\globin\poly\A
     repeat_region   1..130
                     /vntifkey="34"
                     /label=5'\ITR
     repeat_region   complement(3358..3487)
                     /vntifkey="34"
                     /label=3'\ITR
     rep_origin      5281..5869
                     /vntifkey="33"
                     /label=Origin\of\replication
     misc_marker     4250..5107
                     /vntifkey="22"
                     /label=AP(R)
     rep_origin      complement(3664..4119)
                     /vntifkey="33"
                     /label=f1\ori
     misc_feature    226..1404
                     /vntifkey="21"
                     /label=EF1alpha
     misc_marker     2493..3088
                     /vntifkey="22"
                     /label=WPRE
BASE COUNT     1365 a      1707 c      1693 g      1543 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag ggtaatgggg 
      181 atcctctaga actatagcta gaattctagt ggatcccccc gcgccggctc cggtgcccgt 
      241 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat 
      301 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg 
      361 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac 
      421 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc 
      481 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg 
      541 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct 
      601 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc 
      661 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta 
      721 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta 
      781 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg 
      841 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga 
      901 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg 
      961 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa 
     1021 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga 
     1081 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct 
     1141 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt 
     1201 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact 
     1261 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt 
     1321 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt 
     1381 tttcttccat ttcaggtgtc gtgagctaga gctttattgc ggtagtttat cacagttaaa 
     1441 ttgctaacgc agtcagtgct tctgacacaa cagtctcgaa cttaagctgc agaagttggt 
     1501 cgtgaggcac tgggcaggta agtatcaagg ttacaagaca ggtttaagga gaccaataga 
     1561 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta 
     1621 ctgacatcca ctttgccttt ctctccacag gtgtccactc ccagttcaat tacagctctt 
     1681 aaggctagag tacttaatac gactcactat aggctagcct cgagaattgt acaattcacg 
     1741 cgggccgcca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 
     1801 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat 
     1861 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc 
     1921 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac 
     1981 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc 
     2041 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc 
     2101 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 
     2161 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag 
     2221 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg 
     2281 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc 
     2341 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat 
     2401 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg 
     2461 tacaagtaat aagctcgcgt gatcaagctt atcgataatc aacctctgga ttacaaaatt 
     2521 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 
     2581 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 
     2641 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 
     2701 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 
     2761 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 
     2821 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 
     2881 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg 
     2941 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 
     3001 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg 
     3061 atctcccttt gggccgcctc cccgcatcga taccgtcgac ccgggcggcc tcgaggacgg 
     3121 ggtgaactac gcctgaggat ccgatctttt tccctctgcc aaaaattatg gggacatcat 
     3181 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt 
     3241 gtgttggaat tttttgtgtc tctcactcgg aagcaattcg ttgatctgaa tttcgaccac 
     3301 ccataatacc cattaccctg gtagataagt agcatggcgg gttaatcatt aactacaagg 
     3361 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg 
     3421 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag 
     3481 cgcgcagcct taattaacct aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa 
     3541 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta 
     3601 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat 
     3661 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 
     3721 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 
     3781 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 
     3841 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 
     3901 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 
     3961 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 
     4021 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 
     4081 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa 
     4141 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 
     4201 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca 
     4261 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca 
     4321 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta 
     4381 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt 
     4441 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc 
     4501 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc 
     4561 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc 
     4621 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa 
     4681 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga 
     4741 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat 
     4801 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca 
     4861 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc 
     4921 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat 
     4981 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag 
     5041 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa 
     5101 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca 
     5161 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 
     5221 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 
     5281 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 
     5341 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 
     5401 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 
     5461 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 
     5521 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 
     5581 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 
     5641 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 
     5701 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 
     5761 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 
     5821 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 
     5881 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 
     5941 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 
     6001 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat 
     6061 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 
     6121 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 
     6181 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 
     6241 ataacaattt cacacaggaa acagctatga ccatgattac gccagattta attaaggcct 
     6301 taattagg 
//
