LOCUS       pAAV.GFAP.EGFP.W        7147 bp    DNA     circular     23-OCT-2017
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11996091|
COMMENT     VNTDBDATE|-11996091|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV.GFAP.EGFP.WPRE.hGH(p2407)|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Lynne Gallery|
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COMMENT                          "Currently local object. Original author: Lynne Gallery" 
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FEATURES             Location/Qualifiers
     CDS             5456..6316
                     /vntifkey="4"
                     /label=Amp-R
     LTR             1..130
                     /vntifkey="19"
                     /label=ITR
     LTR             complement(4399..4528)
                     /vntifkey="19"
                     /label=ITR
     insertion_seq   3881..4359
                     /vntifkey="14"
                     /label=hGH\polyA
     misc_marker     3265..3852
                     /vntifkey="22"
                     /label=WPRE
     misc_feature    4631..4937
                     /vntifkey="21"
                     /label=f1_origin
     misc_feature    6471..7090
                     /vntifkey="21"
                     /label=pBR322_origin
     promoter        308..2514
                     /vntifkey="29"
                     /label=GFAP\promoter
     insertion_seq   145..307
                     /vntifkey="14"
                     /label=Insertion
     CDS             2527..3242
                     /vntifkey="4"
                     /label=eGFP
BASE COUNT     1623 a      1968 c      1933 g      1623 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct gcggccgcac gcgtttttta aaagaaaagg ggggattggg gggtacagtg 
      181 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac 
      241 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt 
      301 taattaaccc acctccctct ctgtgctggg actcacagag ggagacctca ggaggcagtc 
      361 tgtccatcac atgtccaaat gcagagcata ccctgggctg ggcgcagtgg cgcacaactg 
      421 taattccagc actttgggag gctgatgtgg aaggatcact tgagcccaga agttctagac 
      481 cagcctgggc aacatggcaa gaccctatct ctacaaaaaa agttaaaaaa tcagccacgt 
      541 gtggtgacac acacctgtag tcccagctat tcaggaggct gaggtgaggg gatcacttaa 
      601 ggctgggagg ttgaggctgc agtgagtcgt ggttgcgcca ctgcactcca gcctgggcaa 
      661 cagtgagacc ctgtctcaaa agacaaaaaa aaaaaaaaaa aaaaaaagaa catatcctgg 
      721 tgtggagtag gggacgctgc tctgacagag gctcgggggc ctgagctggc tctgtgagct 
      781 ggggaggagg cagacagcca ggccttgtct gcaagcagac ctggcagcat tgggctggcc 
      841 gccccccagg gcctcctctt catgcccagt gaatgactca ccttggcaca gacacaatgt 
      901 tcggggtggg cacagtgcct gcttcccgcc gcaccccagc ccccctcaaa tgccttccga 
      961 gaagcccatt gagcaggggg cttgcattgc accccagcct gacagcctgg catcttggga 
     1021 taaaagcagc acagccccct aggggctgcc cttgctgtgt ggcgccaccg gcggtggaga 
     1081 acaaggctct attcagcctg tgcccaggaa aggggatcag gggatgccca ggcatggaca 
     1141 gtgggtggca gggggggaga ggagggctgt ctgcttccca gaagtccaag gacacaaatg 
     1201 ggtgagggga ctgggcaggg ttctgaccct gtgggaccag agtggagggc gtagatggac 
     1261 ctgaagtctc cagggacaac agggcccagg tctcaggctc ctagttgggc ccagtggctc 
     1321 cagcgtttcc aaacccatcc atccccagag gttcttccca tctctccagg ctgatgtgtg 
     1381 ggaactcgag gaaataaatc tccagtggga gacggagggg tggccaggga aacggggcgc 
     1441 tgcaggaata aagacgagcc agcacagcca gctcatgtgt aacggctttg tggagctgtc 
     1501 aaggcctggt ctctgggaga gaggcacagg gaggccagac aaggaagggg tgacctggag 
     1561 ggacagatcc aggggctaaa gtcctgataa ggcaagagag tgccggcccc ctcttgccct 
     1621 atcaggacct ccactgccac atagaggcca tgattgaccc ttagacaaag ggctggtgtc 
     1681 caatcccagc ccccagcccc agaactccag ggaatgaatg ggcagagagc aggaatgtgg 
     1741 gacatctgtg ttcaagggaa ggactccagg agtctgctgg gaatgaggcc tagtaggaaa 
     1801 tgaggtggcc cttgagggta cagaacaggt tcattcttcg ccaaattccc agcaccttgc 
     1861 aggcacttac agctgagtga gataatgcct gggttatgaa atcaaaaagt tggaaagcag 
     1921 gtcagaggtc atctggtaca gcccttcctt cccttttttt tttttttttt ttgtgagaca 
     1981 aggtctctct ctgttgccca ggctggagtg gcgcaaacac agctcactgc agcctcaacc 
     2041 tactgggctc aagcaatcct ccagcctcag cctcccaaag tgctgggatt acaagcatga 
     2101 gccaccccac tcagcccttt ccttcctttt taattgatgc ataataattg taagtattca 
     2161 tcatggtcca accaaccctt tcttgaccca ccttcctaga gagagggtcc tcttgcttca 
     2221 gcggtcaggg ccccagaccc atggtctggc tccaggtacc acctgcctca tgcaggagtt 
     2281 ggcgtgccca ggaagctctg cctctgggca cagtgacctc agtggggtga ggggagctct 
     2341 ccccatagct gggctgcggc ccaaccccac cccctcaggc tatgccaggg ggtgttgcca 
     2401 ggggcacccg ggcatcgcca gtctagccca ctccttcata aagccctcgc atcccaggag 
     2461 cgagcagagc cagagcaggt tggagaggag acgcatcacc tccgctgctc gccgggatgg 
     2521 ccgccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc 
     2581 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca 
     2641 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc 
     2701 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca 
     2761 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca 
     2821 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca 
     2881 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg 
     2941 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga 
     3001 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc 
     3061 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca 
     3121 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca 
     3181 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca 
     3241 agtaataagc tagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac 
     3301 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt 
     3361 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt 
     3421 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt 
     3481 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg 
     3541 aactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg 
     3601 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc 
     3661 atcgtccttt ccttggctgc tcgcctatgt tgccacctgg attctgcgcg ggacgtcctt 
     3721 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc 
     3781 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc 
     3841 cgcctccccg catcgatacc gagcgctgct cgagagatct acgggtggca tccctgtgac 
     3901 ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc 
     3961 ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg 
     4021 tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg 
     4081 gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc 
     4141 tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc 
     4201 atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc 
     4261 aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg 
     4321 ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttgta ggtaaccacg 
     4381 tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg 
     4441 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg 
     4501 cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc 
     4561 tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc 
     4621 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact 
     4681 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc 
     4741 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt 
     4801 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc 
     4861 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt 
     4921 gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat 
     4981 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 
     5041 ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga 
     5101 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc 
     5161 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg 
     5221 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct 
     5281 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg 
     5341 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc 
     5401 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag 
     5461 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt 
     5521 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt 
     5581 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga 
     5641 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat 
     5701 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga 
     5761 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag 
     5821 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg 
     5881 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg 
     5941 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt 
     6001 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg 
     6061 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc 
     6121 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg 
     6181 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac 
     6241 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact 
     6301 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa 
     6361 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa 
     6421 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg 
     6481 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc 
     6541 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac 
     6601 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca 
     6661 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt 
     6721 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc 
     6781 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg 
     6841 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc 
     6901 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac 
     6961 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct 
     7021 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc 
     7081 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtcctg 
     7141 caggcag 
//
