LOCUS       pAAV.GFAP.Cre.WP        7465 bp    DNA     circular     22-OCT-2017
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11995027|
COMMENT     VNTDBDATE|-11995027|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV.GFAP.Cre.WPRE.hGH (p2408) Q|
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FEATURES             Location/Qualifiers
     CDS             5774..6634
                     /vntifkey="4"
                     /label=Amp-R
     LTR             1..130
                     /vntifkey="19"
                     /label=ITR
     LTR             complement(4717..4846)
                     /vntifkey="19"
                     /label=ITR
     insertion_seq   4199..4677
                     /vntifkey="14"
                     /label=hGH\polyA
     misc_marker     3583..4170
                     /vntifkey="22"
                     /label=WPRE
     misc_feature    4949..5255
                     /vntifkey="21"
                     /label=f1_origin
     misc_feature    6789..7408
                     /vntifkey="21"
                     /label=pBR322_origin
     promoter        308..2514
                     /vntifkey="29"
                     /label=GFAP\promoter
     insertion_seq   145..307
                     /vntifkey="14"
                     /label=Insertion
     CDS             2536..3564
                     /vntifkey="4"
                     /label=Cre
BASE COUNT     1710 a      1969 c      2015 g      1771 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct gcggccgcac gcgtttttta aaagaaaagg ggggattggg gggtacagtg 
      181 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac 
      241 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt 
      301 taattaaccc acctccctct ctgtgctggg actcacagag ggagacctca ggaggcagtc 
      361 tgtccatcac atgtccaaat gcagagcata ccctgggctg ggcgcagtgg cgcacaactg 
      421 taattccagc actttgggag gctgatgtgg aaggatcact tgagcccaga agttctagac 
      481 cagcctgggc aacatggcaa gaccctatct ctacaaaaaa agttaaaaaa tcagccacgt 
      541 gtggtgacac acacctgtag tcccagctat tcaggaggct gaggtgaggg gatcacttaa 
      601 ggctgggagg ttgaggctgc agtgagtcgt ggttgcgcca ctgcactcca gcctgggcaa 
      661 cagtgagacc ctgtctcaaa agacaaaaaa aaaaaaaaaa aaaaaaagaa catatcctgg 
      721 tgtggagtag gggacgctgc tctgacagag gctcgggggc ctgagctggc tctgtgagct 
      781 ggggaggagg cagacagcca ggccttgtct gcaagcagac ctggcagcat tgggctggcc 
      841 gccccccagg gcctcctctt catgcccagt gaatgactca ccttggcaca gacacaatgt 
      901 tcggggtggg cacagtgcct gcttcccgcc gcaccccagc ccccctcaaa tgccttccga 
      961 gaagcccatt gagcaggggg cttgcattgc accccagcct gacagcctgg catcttggga 
     1021 taaaagcagc acagccccct aggggctgcc cttgctgtgt ggcgccaccg gcggtggaga 
     1081 acaaggctct attcagcctg tgcccaggaa aggggatcag gggatgccca ggcatggaca 
     1141 gtgggtggca gggggggaga ggagggctgt ctgcttccca gaagtccaag gacacaaatg 
     1201 ggtgagggga ctgggcaggg ttctgaccct gtgggaccag agtggagggc gtagatggac 
     1261 ctgaagtctc cagggacaac agggcccagg tctcaggctc ctagttgggc ccagtggctc 
     1321 cagcgtttcc aaacccatcc atccccagag gttcttccca tctctccagg ctgatgtgtg 
     1381 ggaactcgag gaaataaatc tccagtggga gacggagggg tggccaggga aacggggcgc 
     1441 tgcaggaata aagacgagcc agcacagcca gctcatgtgt aacggctttg tggagctgtc 
     1501 aaggcctggt ctctgggaga gaggcacagg gaggccagac aaggaagggg tgacctggag 
     1561 ggacagatcc aggggctaaa gtcctgataa ggcaagagag tgccggcccc ctcttgccct 
     1621 atcaggacct ccactgccac atagaggcca tgattgaccc ttagacaaag ggctggtgtc 
     1681 caatcccagc ccccagcccc agaactccag ggaatgaatg ggcagagagc aggaatgtgg 
     1741 gacatctgtg ttcaagggaa ggactccagg agtctgctgg gaatgaggcc tagtaggaaa 
     1801 tgaggtggcc cttgagggta cagaacaggt tcattcttcg ccaaattccc agcaccttgc 
     1861 aggcacttac agctgagtga gataatgcct gggttatgaa atcaaaaagt tggaaagcag 
     1921 gtcagaggtc atctggtaca gcccttcctt cccttttttt tttttttttt ttgtgagaca 
     1981 aggtctctct ctgttgccca ggctggagtg gcgcaaacac agctcactgc agcctcaacc 
     2041 tactgggctc aagcaatcct ccagcctcag cctcccaaag tgctgggatt acaagcatga 
     2101 gccaccccac tcagcccttt ccttcctttt taattgatgc ataataattg taagtattca 
     2161 tcatggtcca accaaccctt tcttgaccca ccttcctaga gagagggtcc tcttgcttca 
     2221 gcggtcaggg ccccagaccc atggtctggc tccaggtacc acctgcctca tgcaggagtt 
     2281 ggcgtgccca ggaagctctg cctctgggca cagtgacctc agtggggtga ggggagctct 
     2341 ccccatagct gggctgcggc ccaaccccac cccctcaggc tatgccaggg ggtgttgcca 
     2401 ggggcacccg ggcatcgcca gtctagccca ctccttcata aagccctcgc atcccaggag 
     2461 cgagcagagc cagagcaggt tggagaggag acgcatcacc tccgctgctc gccgggatcg 
     2521 cgggaattcg ccaccatgtc caatttactg accgtacacc aaaatttgcc tgcattaccg 
     2581 gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg acatgttcag ggatcgccag 
     2641 gcgttttctg agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg ggcggcatgg 
     2701 tgcaagttga ataaccggaa atggtttccc gcagaacctg aagatgttcg cgattatctt 
     2761 ctatatcttc aggcgcgcgg tctggcagta aaaactatcc agcaacattt gggccagcta 
     2821 aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc tgtttcactg 
     2881 gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta 
     2941 gcgttcgaac gcactgattt cgaccaggtt cgttcactca tggaaaatag cgatcgctgc 
     3001 caggatatac gtaatctggc atttctgggg attgcttata acaccctgtt acgtatagcc 
     3061 gaaattgcca ggatcagggt taaagatatc tcacgtactg acggtgggag aatgttaatc 
     3121 catattggca gaacgaaaac gctggttagc accgcaggtg tagagaaggc acttagcctg 
     3181 ggggtaacta aactggtcga gcgatggatt tccgtctctg gtgtagctga tgatccgaat 
     3241 aactacctgt tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc caccagccag 
     3301 ctatcaactc gcgccctgga agggattttt gaagcaactc atcgattgat ttacggcgct 
     3361 aaggatgact ctggtcagag atacctggcc tggtctggac acagtgcccg tgtcggagcc 
     3421 gcgcgagata tggcccgcgc tggagtttca ataccggaga tcatgcaagc tggtggctgg 
     3481 accaatgtaa atattgtcat gaactatatc cgtaacctgg atagtgaaac aggggcaatg 
     3541 gtgcgcctgc tggaagatgg cgattagcca gcttatcgat aatcaacctc tggattacaa 
     3601 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 
     3661 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 
     3721 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 
     3781 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 
     3841 ctgtcagctc ctttccggaa ctttcgcttt ccccctccct attgccacgg cggaactcat 
     3901 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 
     3961 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctatgttg ccacctggat 
     4021 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 
     4081 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag 
     4141 tcggatctcc ctttgggccg cctccccgca tcgataccga gcgctgctcg agagatctac 
     4201 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca 
     4261 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc 
     4321 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca 
     4381 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg 
     4441 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg 
     4501 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg 
     4561 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct 
     4621 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg 
     4681 attttgtagg taaccacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg 
     4741 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac 
     4801 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc 
     4861 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa 
     4921 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 
     4981 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 
     5041 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 
     5101 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc 
     5161 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 
     5221 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc 
     5281 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 
     5341 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct 
     5401 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc 
     5461 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt 
     5521 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa 
     5581 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac 
     5641 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 
     5701 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 
     5761 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 
     5821 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 
     5881 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 
     5941 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg 
     6001 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc 
     6061 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 
     6121 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact 
     6181 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca 
     6241 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 
     6301 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 
     6361 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 
     6421 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 
     6481 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 
     6541 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 
     6601 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 
     6661 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 
     6721 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 
     6781 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 
     6841 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 
     6901 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt 
     6961 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 
     7021 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 
     7081 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 
     7141 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 
     7201 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 
     7261 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 
     7321 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 
     7381 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 
     7441 ttttgctcac atgtcctgca ggcag 
//
