LOCUS       pENN.AAV.CamKII.        7496 bp    DNA     circular     22-OCT-2017
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11995036|
COMMENT     VNTDBDATE|-11995036|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (P2521).|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Mitesh Mehta|
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COMMENT        #263=(CSampFrag "pENN.AAV.CamKII.hGHintron.GFP-Cre.WPRE.SV40 (P2521)" 
COMMENT              4053 341 "BglI" 4394 "BglI" (LOGPEN 0 0 0) 1.06056e-316 
COMMENT              1.71931e-231) 
COMMENT        #264=(CSampFrag "pENN.AAV.CamKII.hGHintron.GFP-Cre.WPRE.SV40 (P2521)" 
COMMENT              1714 4394 "BglI" 6108 "BglI" (LOGPEN 0 0 0) 1.39041e-316 
COMMENT              1.82051e-300) 
COMMENT        #265=(CSampFrag "pENN.AAV.CamKII.hGHintron.GFP-Cre.WPRE.SV40 (P2521)" 
COMMENT              1420 6108 "BglI" 32 "BglI" (LOGPEN 0 0 0) 9.5701e-315 1.46115e-300) 
COMMENT        #266=(CSampFrag "pENN.AAV.CamKII.hGHintron.GFP-Cre.WPRE.SV40 (P2521)" 
COMMENT              309 32 "BglI" 341 "BglI" (LOGPEN 0 0 0) 1.39523e-316 1.9369e-300))))
FEATURES             Location/Qualifiers
     misc_feature    175..1247
                     /vntifkey="21"
                     /label=CaMKIIa\Promoter
     intron          1372..1643
                     /vntifkey="15"
                     /label=hGHintron1\(AF264722.1)
     misc_marker     3510..4097
                     /vntifkey="22"
                     /label=WPRE
     LTR             1..130
                     /vntifkey="19"
                     /label=5'ITR
     polyA_signal    4129..4188
                     /vntifkey="25"
                     /label=SV40\polyA
     CDS             2457..3487
                     /vntifkey="4"
                     /label=Cre
     repeat_region   complement(4292..4421)
                     /vntifkey="34"
                     /label=ITR
     CDS             5438..6295
                     /vntifkey="4"
                     /label=Amp-R
     misc_feature    6469..7057
                     /vntifkey="21"
                     /label=Origin
     CDS             1679..2395
                     /vntifkey="4"
                     /label=GFP
BASE COUNT     1766 a      1970 c      1988 g      1772 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct gcggccgcac gcgtactagg tagccatgct ctaggaagat ctaacattat 
      181 ggccttaggt cacttcatct ccatggggtt cttcttctga ttttctagaa aatgagatgg 
      241 gggtgcagag agcttcctca gtgacctgcc cagggtcaca tcagaaatgt cagagctaga 
      301 acttgaactc agattactaa tcttaaattc catgccttgg gggcatgcaa gtacgatata 
      361 cagaaggagt gaactcatta gggcagatga ccaatgagtt taggaaagaa gagtccaggg 
      421 cagggtacat ctacaccacc cgcccagccc tgggtgagtc cagccacgtt cacctcatta 
      481 tagttgcctc tctccagtcc taccttgacg ggaagcacaa gcagaaactg ggacaggagc 
      541 cccaggagac caaatcttca tggtccctct gggaggatgg gtggggagag ctgtggcaga 
      601 ggcctcagga ggggccctgc tgctcagtgg tgacagatag gggtgagaaa gcagacagag 
      661 tcattccgtc agcattctgg gtctgtttgg tacttcttct cacgctaagg tggcggtgtg 
      721 atatgcacaa tggctaaaaa gcagggagag ctggaaagaa acaaggacag agacagaggc 
      781 caagtcaacc agaccaattc ccagaggaag caaagaaacc attacagaga ctacaagggg 
      841 gaagggaagg agagatgaat tagcttcccc tgtaaacctt agaacccagc tgttgccagg 
      901 gcaacggggc aatacctgtc tcttcagagg agatgaagtt gccagggtaa ctacatcctg 
      961 tctttctcaa ggaccatccc agaatgtggc acccactagc cgttaccata gcaactgcct 
     1021 ctttgcccca cttaatccca tcccgtctgt taaaagggcc ctatagttgg aggtggggga 
     1081 ggtaggaaga gcgatgatca cttgtggact aagtttgttc gcatcccctt ctccaacccc 
     1141 ctcagtacat caccctgggg gaacagggtc cacttgctcc tgggcccaca cagtcctgca 
     1201 gtattgtgta tataaggcca gggcaaagag gagcaggttt taaagtgaaa ggcaggcagg 
     1261 tgttggggag gcagttaccg gggcaacggg aacagggcgt ttcggaggtg gttgccatgg 
     1321 ggacctggat gctgacgaag gctcgcggga ccgatccagc ctccgtaccg gttcgaacag 
     1381 gtaagcgccc ctaaaatccc tttgggcaca atgtgtcctg aggggagagg cagcgacctg 
     1441 tagatgggac gggggcacta accctcaggt ttggggcttc tgaatgtgag tatcgccatg 
     1501 taagcccagt atttggccaa tctcagaaag ctcctggtcc ctggagggat ggagagagaa 
     1561 aaacaaacag ctcctggagc agggagagtg ctggcctctt gctctccggc tccctctgtt 
     1621 gccctctggt ttctccccag gttcgaatct agaggatccc cgggtaccgg tcgccaccat 
     1681 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg 
     1741 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg 
     1801 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct 
     1861 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca 
     1921 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt 
     1981 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt 
     2041 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa 
     2101 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg 
     2161 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga 
     2221 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta 
     2281 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct 
     2341 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtccgg 
     2401 agagcaaaag ctgatttctg aggaggatct gggaggaccc aagaagaaga ggaaggtgtc 
     2461 caatttactg accgtacacc aaaatttgcc tgcattaccg gtcgatgcaa cgagtgatga 
     2521 ggttcgcaag aacctgatgg acatgttcag ggatcgccag gcgttttctg agcatacctg 
     2581 gaaaatgctt ctgtccgttt gccggtcgtg ggcggcatgg tgcaagttga ataaccggaa 
     2641 atggtttccc gcagaacctg aagatgttcg cgattatctt ctatatcttc aggcgcgcgg 
     2701 tctggcagta aaaactatcc agcaacattt gggccagcta aacatgcttc atcgtcggtc 
     2761 cgggctgcca cgaccaagtg acagcaatgc tgtttcactg gttatgcggc ggatccgaaa 
     2821 agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta gcgttcgaac gcactgattt 
     2881 cgaccaggtt cgttcactca tggaaaatag cgatcgctgc caggatatac gtaatctggc 
     2941 atttctgggg attgcttata acaccctgtt acgtatagcc gaaattgcca ggatcagggt 
     3001 taaagatatc tcacgtactg acggtgggag aatgttaatc catattggca gaacgaaaac 
     3061 gctggttagc accgcaggtg tagagaaggc acttagcctg ggggtaacta aactggtcga 
     3121 gcgatggatt tccgtctctg gtgtagctga tgatccgaat aactacctgt tttgccgggt 
     3181 cagaaaaaat ggtgttgccg cgccatctgc caccagccag ctatcaactc gcgccctgga 
     3241 agggattttt gaagcaactc atcgattgat ttacggcgct aaggatgact ctggtcagag 
     3301 atacctggcc tggtctggac acagtgcccg tgtcggagcc gcgcgagata tggcccgcgc 
     3361 tggagtttca ataccggaga tcatgcaagc tggtggctgg accaatgtaa atattgtcat 
     3421 gaactatatc cgtaacctgg atagtgaaac aggggcaatg gtgcgcctgc tggaagatgg 
     3481 cgattagctc gaatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga 
     3541 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 
     3601 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 
     3661 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 
     3721 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 
     3781 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 
     3841 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 
     3901 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 
     3961 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 
     4021 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt 
     4081 tgggccgcct ccccgcatcg ataccgtcga tcgagaaatc gatcgcccaa cttgtttatt 
     4141 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 
     4201 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 
     4261 atctccggac acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact 
     4321 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg 
     4381 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gccttaatta acctaattca 
     4441 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 
     4501 cttgcagcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg 
     4561 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag 
     4621 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc 
     4681 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc 
     4741 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa 
     4801 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg 
     4861 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac 
     4921 actcaactct atctcgggct attcttttga tttataaggg attttgccga tttcggtcta 
     4981 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac 
     5041 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca 
     5101 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc 
     5161 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc 
     5221 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt 
     5281 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac 
     5341 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 
     5401 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 
     5461 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 
     5521 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 
     5581 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag 
     5641 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca 
     5701 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 
     5761 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 
     5821 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 
     5881 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 
     5941 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 
     6001 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 
     6061 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 
     6121 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 
     6181 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 
     6241 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 
     6301 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 
     6361 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 
     6421 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 
     6481 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 
     6541 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 
     6601 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt 
     6661 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 
     6721 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 
     6781 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 
     6841 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 
     6901 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 
     6961 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 
     7021 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 
     7081 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 
     7141 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac 
     7201 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc 
     7261 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 
     7321 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc 
     7381 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca 
     7441 cacaggaaac agctatgacc atgattacgc cagatttaat taaggcctta attagg 
//
