LOCUS       pENN.AAV.CAG.tdT        6146 bp    DNA     circular     23-OCT-2017
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11995778|
COMMENT     VNTDBDATE|-11995778|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pENN.AAV.CAG.tdTomato.WPRE.SV40 (p3365) Q|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Ruben Hovhannisyan|
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FEATURES             Location/Qualifiers
     polyA_signal    3200..3438
                     /vntifkey="25"
                     /label=SV40
     misc_marker     2588..3181
                     /vntifkey="22"
                     /label=WPRE
     LTR             1..130
                     /vntifkey="19"
                     /label=5'\ITR
     LTR             complement(3504..3633)
                     /vntifkey="19"
                     /label=3'\ITR
     misc_feature    438..719
                     /vntifkey="21"
                     /label=chicken\beta-actin\promoter
     enhancer        193..435
                     /vntifkey="9"
                     /label=CMV\enhancer
     intron          719..949
                     /vntifkey="15"
                     /label=chicken\beta-actin\intron
     misc_feature    5620..6075
                     /vntifkey="21"
                     /label=f1\ori
     CDS             4629..5489
                     /vntifkey="4"
                     /label=Amp-R
     misc_feature    complement(3870..4458)
                     /vntifkey="21"
                     /label=ori
     CDS             1140..2570
                     /vntifkey="4"
                     /label=tdTomato
BASE COUNT     1358 a      1722 c      1689 g      1377 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 
      181 aggaagagta ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 
      241 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 
      301 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 
      361 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt 
      421 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc 
      481 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat 
      541 gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg 
      601 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc 
      661 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg 
      721 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc 
      781 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 
      841 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag 
      901 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggc tgtccgcggg 
      961 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg 
     1021 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg 
     1081 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattggat cccgccacca 
     1141 tggtgagcaa gggcgaggag gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg 
     1201 gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg 
     1261 gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca 
     1321 tcctgtcccc ccagttcatg tacggctcca aggcgtacgt gaagcacccc gccgacatcc 
     1381 ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg 
     1441 aggacggcgg tctggtgacc gtgacccagg actcctccct gcaggacggc acgctgatct 
     1501 acaaggtgaa gatgcgcggc accaacttcc cccccgacgg ccccgtaatg cagaagaaga 
     1561 ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg 
     1621 agatccacca ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagacca 
     1681 tctacatggc caagaagccc gtgcaactgc ccggctacta ctacgtggac accaagctgg 
     1741 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc tccgagggcc 
     1801 gccaccacct gttcctgggg catggcaccg gcagcaccgg cagcggcagc tccggcaccg 
     1861 cctcctccga ggacaacaac atggccgtca tcaaagagtt catgcgcttc aaggtgcgca 
     1921 tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct 
     1981 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct 
     2041 gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag caccccgccg 
     2101 acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga 
     2161 acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag gacggcacgc 
     2221 tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc gtaatgcaga 
     2281 agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga 
     2341 agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccgctacctg gtggagttca 
     2401 agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac gtggacacca 
     2461 agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgctccg 
     2521 agggccgcca ccacctgttc ctgtacggca tggacgagct gtacaagtaa gaattcgaag 
     2581 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac 
     2641 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt 
     2701 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat 
     2761 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca 
     2821 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc 
     2881 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg 
     2941 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct 
     3001 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct 
     3061 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt 
     3121 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat 
     3181 cgataccgtc gacccgggcg gccgcttcga gcagacatga taagatacat tgatgagttt 
     3241 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct 
     3301 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt 
     3361 cattttatgt ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc 
     3421 tacaaatgtg gtaaaatcga taaggatctt cctagagcat ggctacgtag ataagtagca 
     3481 tggcgggtta atcattaact acaaggaacc cctagtgatg gagttggcca ctccctctct 
     3541 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc 
     3601 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcatt aatgaatcgg ccaacgcgcg 
     3661 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 
     3721 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 
     3781 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 
     3841 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 
     3901 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 
     3961 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 
     4021 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 
     4081 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 
     4141 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 
     4201 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 
     4261 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 
     4321 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 
     4381 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 
     4441 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 
     4501 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 
     4561 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 
     4621 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 
     4681 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 
     4741 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 
     4801 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 
     4861 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 
     4921 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 
     4981 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 
     5041 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 
     5101 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 
     5161 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 
     5221 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 
     5281 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 
     5341 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 
     5401 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 
     5461 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 
     5521 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 
     5581 ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt 
     5641 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa 
     5701 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag 
     5761 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg 
     5821 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga 
     5881 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg 
     5941 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg 
     6001 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc 
     6061 cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg 
     6121 tgcgggcctc ttcgctatta cgccag 
//
