LOCUS       pENN.AAV.CB7.CI.        6479 bp    DNA     circular     23-OCT-2017
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11996029|
COMMENT     VNTDBDATE|-11996029|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pENN.AAV.CB7.CI.mCerulean.WPRE.RBG (p3248)|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Ruben Hovhannisyan|
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COMMENT                          "Currently local object. Original author: Ruben Hovhannisyan" 
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FEATURES             Location/Qualifiers
     promoter        582..863
                     /vntifkey="29"
                     /label=CB\promoter
     intron          956..1928
                     /vntifkey="15"
                     /label=chicken\beta-actin\intron
     TATA_signal     836..839
                     /vntifkey="41"
                     /label=TATA
     polyA_signal    3314..3440
                     /vntifkey="25"
                     /label=Rabbit\globin\poly\A
     repeat_region   1..130
                     /vntifkey="34"
                     /label=5'\ITR
     repeat_region   complement(3529..3658)
                     /vntifkey="34"
                     /label=3'\ITR
     rep_origin      5452..6040
                     /vntifkey="33"
                     /label=Origin\of\replication
     misc_marker     4421..5278
                     /vntifkey="22"
                     /label=AP(R)
     rep_origin      complement(3835..4290)
                     /vntifkey="33"
                     /label=f1\ori
     repeat_region   198..579
                     /vntifkey="34"
                     /label=CMV\IE\enhancer
     misc_marker     2698..3293
                     /vntifkey="22"
                     /label=WPRE
     CDS             1960..2679
                     /vntifkey="4"
                     /label=mCerulean
BASE COUNT     1312 a      1812 c      1853 g      1502 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag ggtaatgggg 
      181 atcctctaga actatagcta gtcgacattg attattgact agttattaat agtaatcaat 
      241 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 
      301 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 
      361 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 
      421 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 
      481 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 
      541 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac 
      601 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat 
      661 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg 
      721 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca 
      781 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa 
      841 aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc 
      901 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag 
      961 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt 
     1021 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg 
     1081 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc 
     1141 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt 
     1201 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa 
     1261 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt 
     1321 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg 
     1381 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca 
     1441 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg 
     1501 cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt 
     1561 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa 
     1621 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg 
     1681 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc 
     1741 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc 
     1801 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc 
     1861 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt 
     1921 tggcaaagaa ttcacgcgct agcgctaccg gtcgccacca tggtgagcaa gggcgaggag 
     1981 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag 
     2041 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc 
     2101 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg 
     2161 ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc 
     2221 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac 
     2281 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag 
     2341 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caacgccatc 
     2401 agcgacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag 
     2461 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc 
     2521 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag 
     2581 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc 
     2641 gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccatca agcttatcga 
     2701 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 
     2761 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 
     2821 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 
     2881 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 
     2941 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 
     3001 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 
     3061 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct 
     3121 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 
     3181 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 
     3241 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg 
     3301 tcgatgagga tccgatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct 
     3361 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa 
     3421 ttttttgtgt ctctcactcg gaagcaattc gttgatctga atttcgacca cccataatac 
     3481 ccattaccct ggtagataag tagcatggcg ggttaatcat taactacaag gaacccctag 
     3541 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa 
     3601 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagcc 
     3661 ttaattaacc taattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg 
     3721 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag 
     3781 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc 
     3841 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 
     3901 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 
     3961 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 
     4021 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 
     4081 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 
     4141 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 
     4201 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 
     4261 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg 
     4321 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 
     4381 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg 
     4441 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac 
     4501 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact 
     4561 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat 
     4621 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga 
     4681 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac 
     4741 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat 
     4801 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac 
     4861 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct 
     4921 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac 
     4981 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga 
     5041 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg 
     5101 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact 
     5161 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac 
     5221 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta 
     5281 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt 
     5341 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 
     5401 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 
     5461 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 
     5521 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 
     5581 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc 
     5641 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 
     5701 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 
     5761 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 
     5821 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 
     5881 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 
     5941 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 
     6001 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 
     6061 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc 
     6121 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg 
     6181 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc 
     6241 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 
     6301 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca 
     6361 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt 
     6421 tcacacagga aacagctatg accatgatta cgccagattt aattaaggcc ttaattagg 
//
