LOCUS       Exported                4153 bp ds-DNA     circular SYN 12-JAN-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 12, 2018 from SnapGene Viewer 4.1.2
            http://www.snapgene.com
COMMENT     GenBank                4153 bp ds-DNA     linear       15-DEC-2017
            ACCESSION  
            VERSION
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    664..680
                     /gene="T7 promotor"
                     /label=T7 promotor
     misc_feature    682..772
                     /gene="5'UTR"
                     /label=5'UTR
     CDS             806..1156
                     /codon_start=1
                     /gene="VHHGFP4"
                     /label=VHHGFP4
                     /translation="MDQVQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGK
                     EREWVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGF
                     EYWGQGTQVTVSS"
     CDS             1163..1858
                     /codon_start=1
                     /gene="hIgG1-Fc1"
                     /label=hIgG1-Fc1
                     /translation="MVRSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTC
                     VVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYK
                     CKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAV
                     EWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQ
                     KSLSLSPGK"
     misc_feature    1896
                     /gene="Start 3' UTR"
                     /label=Start 3' UTR
     CDS             complement(3357..4153)
                     /gene="AmpR"
                     /label=AmpR
ORIGIN
        1 aaacaggaag gcaaatgccg aaaaaaggga ataagggcga cacggaaatg ttgaatactc
       61 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
      121 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
      181 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
      241 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
      301 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
      361 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
      421 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
      481 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
      541 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      601 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
      661 ttgtaatacg actcactata gggcgaatta attcgagctc ggtacccagc ttgcttgttc
      721 tttttgcaga agctcagaat aaacgctcaa ctttggcaga tcaattcccc ggtgctagcg
      781 ctaccggtct cagatctcga gctcaatgga tcaagtccaa ctggtggagt ctggtggcgc
      841 tttggtgcag ccaggtggct ctctgcgttt gtcctgtgcc gcttctggct tcccagtgaa
      901 ccgctattcc atgcgctggt atcgccaggc tccaggcaaa gagcgtgagt gggtagccgg
      961 tatgtccagc gcgggtgatc gtagctccta tgaagactcc gtgaagggcc gtttcaccat
     1021 cagccgtgac gatgcccgta acacggtgta tctgcaaatg aacagcttga aacctgaaga
     1081 tacggccgtg tattactgta atgtgaacgt gggcttcgag tattggggcc aaggcaccca
     1141 ggtcaccgtc tccagcctgc agatggttag atctgacaaa actcacacat gcccaccgtg
     1201 cccagcacct gaactcctgg ggggaccgtc agtcttcctc ttccccccaa aacccaagga
     1261 caccctcatg atctcccgga cccctgaggt cacatgcgtg gtggtggacg tgagccacga
     1321 agaccctgag gtcaagttca actggtacgt ggacggcgtg gaggtgcata atgccaagac
     1381 aaagccgcgg gaggagcagt acaacagcac gtaccgtgtg gtcagcgtcc tcaccgtcct
     1441 gcaccaggac tggctgaatg gcaaggagta caagtgcaag gtctccaaca aagccctccc
     1501 agcccccatc gagaaaacca tctccaaagc caaagggcag ccccgagaac cacaggtgta
     1561 caccctgccc ccatcccggg aggagatgac caagaaccag gtcagcctga cctgcctggt
     1621 caaaggcttc tatcccagcg acatcgccgt ggagtgggag agcaatgggc agccggagaa
     1681 caactacaag accacgcctc ccgtgctgga ctccgacggc tccttcttcc tctacagcaa
     1741 gctcaccgtg gacaagagca ggtggcagca ggggaacgtc ttctcatgct ccgtgatgca
     1801 cgaggctctg cacaaccact acacgcagaa gagcctctcc ctgtctccgg gtaaatgagc
     1861 gggcccggga tcctgtacaa gtaaagcggc cgctctagat aaatgatagc ttgatctggt
     1921 taccactaaa ccagcctcaa gaacacccga atggagtctc taagctacat aataccaact
     1981 tacactttac aaaatgttgt cccccaaaat gtagccattc gtatctgctc ctaataaaaa
     2041 gaaagtttct tcacattcta aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaccccccc
     2101 cccccccccc tgcaggcgcg ccgccggcga tttaaattaa ttaatctagc ttgagtattc
     2161 tatagtgtca cctaaatagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     2221 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     2281 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     2341 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     2401 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     2461 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     2521 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     2581 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2641 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2701 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2761 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     2821 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2881 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac cttatcgcca
     2941 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3001 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     3061 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3121 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3181 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3241 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3301 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     3361 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     3421 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3481 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     3541 ccagcccgga agggccgagc gcagaagtgg tcctgcaaac tttatccgcc tccatccagt
     3601 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     3661 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     3721 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     3781 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     3841 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     3901 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     3961 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     4021 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     4081 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     4141 tttctgggtg agc
//
