LOCUS       pMSCV_puro_N3FLA        8296 bp ds-DNA     circular     18-DEC-2017
DEFINITION  Retroviral plasmid with multiple cloning site and puromycin
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    MSCV Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6295)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY,
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 6295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 18, 2017 from SnapGene Viewer 3.3.4
            http://www.snapgene.com
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    579..920
                     /note="packaging signal of murine embryonic stem cell
                     virus"
                     /label=MESV Psi
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             987..1403
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /label=gag (truncated)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1333..1355
                     /note="Murine stem cell virus, forward primer. Also called
                     MSCV"
                     /label=pLXSN 5'
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1371..1387
                     /note="Psi packaging signal, forward primer for pBABE
                     vectors"
                     /label=pBABE 5'
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        3439..3938
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label=PGK promoter
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(3474..3497)
                     /note="Murine stem cell virus, reverse primer"
                     /label=MSCV-rev
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(3709..3720)
                     /note="T7 terminator, reverse primer"
                     /label=T7 Term
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     3871..3890
                     /note="Mouse PGK promoter, forward primer"
                     /label=mPGK-F
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             3959..4558
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     R
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     A
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     S APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
                     /label=PuroR
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(3959..3978)
                     /note="Puromycin resistance gene, reverse primer. Also
                     called puro-variant-R"
                     /label=Puro-R
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     4455..4475
                     /note="Puromycin resistance gene, forward primer"
                     /label=Puro-F
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     LTR             4658..5172
                     /note="3' long terminal repeat from murine embryonic stem
                     cell virus"
                     /label=3' LTR
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(5341..5357)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label=M13 rev
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(5341..5357)
                     /note="In lacZ gene. Also called M13-rev"
                     /label=M13 Reverse
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(5354..5376)
                     /note="In lacZ gene"
                     /label=M13/pUC Reverse
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    5365..5381
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /label=lac operator
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(5389..5419)
                     /note="promoter for the E. coli lac operon"
                     /label=lac promoter
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    5434..5455
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label=CAP binding site
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(5572..5589)
                     /note="L4440 vector, forward primer"
                     /label=L4440
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(5743..6331)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label=ori
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(5823..5842)
                     /note="pBR322 origin, forward primer"
                     /label=pBR322ori-F
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(6502..7362)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     I
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     S
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     W
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     A
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     S LIKHW"
                     /label=AmpR
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     7125..7144
                     /note="Ampicillin resistance gene, reverse primer"
                     /label=Amp-R
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(7363..7467)
                     /gene="bla"
                     /label=AmpR promoter
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     7535..7553
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
                     /label=pBRforEco
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(7591..7613)
                     /note="pGEX vectors, reverse primer"
                     /label=pGEX 3'
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     7713..7732
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
                     /label=pRS-marker
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     7926..7948
                     /note="In lacZ gene"
                     /label=M13/pUC Forward
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     8076..8095
                     /note="pBR322 vectors, tet region, downstream of BamHI,
                     reverse primer"
                     /label=pBRrevBam
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     LTR             join(8295..8296,1..515)
                     /note="5' long terminal repeat from murine embryonic stem
                     cell virus"
                     /label=5' LTR
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1422..1442
                     /label=NLS
                     /ApEinfo_fwdcolor=#008000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1509..1514
                     /label=Linker
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1419..1421
                     /label=ATG
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1443..1508
                     /label=3*FLAG
                     /ApEinfo_fwdcolor=#66ccff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1515..3428
                     /label=MYB-FL
                     /ApEinfo_fwdcolor=#ff6666
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatctGCCAT GCCAAAGAAG AAGCGTAAGG
     1441 TAGATTACAA AGACCATGAC GGTGATTATA AAGATCATGA TATCGATTAC AAGGATGACG
     1501 ATGACAAGGG AGGCCTCGAG GCCCGGAGAC CCCGACACAG CATCTACAGT AGCGATGAAG
     1561 ATGATGAAGA CATTGAGATG TGTGACCATG ACTACGATGG GCTGCTGCCC AAATCTGGAA
     1621 AGCGTCACTT GGGGAAAACT AGGTGGACAA GGGAAGAGGA TGAGAAGCTG AAGAAGCTGG
     1681 TGGAACAGAA CGGAACAGAC GACTGGAAAG TCATTGCCAA TTATCTGCCC AACCGGACAG
     1741 ATGTGCAGTG CCAACACCGG TGGCAGAAAG TGCTGAACCC TGAACTCATC AAAGGTCCCT
     1801 GGACCAAAGA AGAAGATCAG AGAGTCATAG AGCTTGTCCA GAAATATGGT CCGAAGCGTT
     1861 GGTCTGTTAT TGCCAAGCAC TTAAAAGGGA GAATTGGAAA GCAGTGTCGG GAGAGGTGGC
     1921 ACAACCATTT GAATCCAGAA GTTAAGAAAA CCTCCTGGAC AGAAGAGGAG GACAGAATCA
     1981 TTTACCAGGC ACACAAGCGT CTGGGGAACA GATGGGCAGA GATCGCAAAG CTGCTGCCCG
     2041 GACGGACTGA TAATGCTATC AAGAACCACT GGAATTCCAC CATGCGTCGC AAGGTGGAAC
     2101 AGGAAGGCTA CCTGCAGGAG CCTTCCAAAG CCAGCCAGAC GCCAGTGGCC ACGAGCTTCC
     2161 AGAAGAACAA TCATTTGATG GGGTTTGGGC ATGCCTCACC TCCATCTCAG CTCTCTCCAA
     2221 GTGGCCAGTC CTCCGTCAAC AGCGAATATC CCTATTACCA CATCGCCGAA GCACAAAACA
     2281 TCTCCAGTCA CGTTCCCTAT CCTGTCGCAT TGCATGTTAA TATAGTCAAC GTCCCTCAGC
     2341 CGGCTGCGGC AGCCATCCAG AGACACTATA ACGACGAAGA CCCTGAGAAG GAAAAGCGAA
     2401 TAAAGGAGCT GGAGTTGCTC CTGATGTCAA CAGAGAACGA GCTGAAGGGA CAGCAGGCAT
     2461 TACCAACACA GAACCACACT TGCAGCTACC CCGGGTGGCA CAGCACCTCC ATTGTGGACC
     2521 AGACCAGACC TCATGGGGAT AGTGCACCTG TTTCCTGTTT GGGAGAACAC CATGCCACCC
     2581 CATCTCTGCC TGCAGATCCC GGCTCCCTAC CTGAAGAAAG TGCCTCACCA GCAAGGTGCA
     2641 TGATCGTCCA CCAGGGCACC ATTCTGGACA ATGTTAAGAA CCTCTTAGAA TTTGCAGAAA
     2701 CACTCCAGTT TATAGATTCT TTCTTGAACA CTTCCAGCAA CCATGAAAGC TCGGGCTTAG
     2761 ATGCACCTAC CTTACCCTCC ACTCCTCTCA TTGGTCACAA ACTGACACCA TGTCGAGACC
     2821 AGACTGTGAA AACCCAGAAG GAAAATTCCA TCTTTAGAAC TCCAGCTATC AAAAGGTCAA
     2881 TCCTCGAAAG CTCTCCTCGA ACTCCCACAC CATTCAAACA TGCCCTTGCA GCTCAAGAAA
     2941 TTAAATACGG TCCCCTGAAG ATGCTACCTC AGACCCCCTC CCATGCAGTG GAGGACCTAC
     3001 AAGATGTGAT TAAGCAGGAA TCGGATGAAT CTGGAATTGT GGCTGAGTTT CAAGAGAGTG
     3061 GACCACCGTT ACTGAAAAAA ATCAAGCAGG AGGTGGAGTC GCCAACTGAG AAATCGGGAA
     3121 ACTTCTTCTG CTCAAACCAC TGGGCAGAGA ACAGCCTGAG CACCCAGCTG TTCTCGCAGG
     3181 CGTCTCCTGT GGCAGATGCC CCAAATATTC TTACAAGCTC TGTTTTAATG ACACCTGTAT
     3241 CAGAAGATGA AGACAATGTC CTCAAAGCCT TTACCGTACC TAAGAACAGG CCCCTGGTGG
     3301 GTCCCTTGCA GCCATGCAGT GGTGCCTGGG AGCCAGCATC CTGTGGGAAG ACAGAGGACC
     3361 AGATGACGGC CTCCGGTCCG GCTCGGAAAT ACGTGAACGC GTTCTCAGCT CGAACTCTGG
     3421 TCATGtgaga attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     3481 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     3541 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     3601 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     3661 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     3721 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     3781 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     3841 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     3901 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     3961 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     4021 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     4081 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     4141 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     4201 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     4261 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     4321 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     4381 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     4441 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     4501 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg
     4561 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcgataaa
     4621 ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt
     4681 tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaatacata actgagaata
     4741 gagaagttca gatcaaggtt aggaacagag agacagcaga atatgggcca aacaggatat
     4801 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt
     4861 cccgccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga
     4921 aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
     4981 cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcctc
     5041 cgatagactg cgtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg
     5101 acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg
     5161 ggggtctttc atgggtaaca gtttcttgaa gttggagaac aacattctga gggtaggagt
     5221 cgaatattaa gtaatcctga ctcaattagc cactgttttg aatccacata ctccaatact
     5281 cctgaaatag ttcattatgg acagcgcaga agagctgggg agaattaatt cgtaatcatg
     5341 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     5401 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     5461 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     5521 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5581 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5641 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5701 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5761 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5821 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5881 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     5941 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6001 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6061 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6121 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6181 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6241 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     6301 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     6361 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     6421 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6481 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6541 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     6601 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     6661 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     6721 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     6781 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     6841 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     6901 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     6961 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     7021 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     7081 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     7141 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     7201 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     7261 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     7321 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     7381 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     7441 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     7501 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     7561 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     7621 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     7681 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     7741 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     7801 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     7861 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     7921 gttttcccag tcacgacgtt gtaaaacgac ggcgcaagga atggtgcatg caaggagatg
     7981 gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc
     8041 atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca
     8101 gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggcgatta
     8161 gtccaatttg ttaaagacag gatatcagtg gtccaggctc tagttttgac tcaacaatat
     8221 caccagctga agcctataga gtacgagcca tagataaaat aaaagatttt atttagtctc
     8281 cagaaaaagg ggggaa    
//
