LOCUS Exported 423 bp ds-DNA circular SYN 11-NOV-2017 DEFINITION clone 4 correct. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 423) AUTHORS Can Buldun TITLE Direct Submission JOURNAL Exported Nov 11, 2017 from SnapGene 4.0.6 http://www.snapgene.com FEATURES Location/Qualifiers source 1..423 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 13..30 /codon_start=1 /product="6xHis affinity tag" /label=6xHis affinity tag /translation="HHHHHH" primer_bind complement(22..75) /label=CB033_AviIns_Rev CDS 40..84 /codon_start=1 /product="peptide tag that allows for enzymatic biotinylation" /label=AviTag /translation="GLNDIFEAQKIEWHE" primer_bind complement(69..98) /label=CB403_202-2_rev primer_bind complement(81..111) /label=CB491_AP+6SnL_rev primer_bind 91..130 /label=CB490_AP+6SnL_fwd primer_bind 99..136 /label=CB413_198_fwd primer_bind 106..142 /label=CB269_+6SnL_fwd CDS 109..420 /codon_start=1 /label=SnoopLigase /translation="VNKNDKKPLRGAVFSLQKQHPDYPDIYGAIDQNGTYQNVRTGEDG KLTFKNLSDGKYRLFENSEPPGYKPVQNKPIVAFQIVNGEVRDVTSIVPPGVPATYEFT " primer_bind 127..142 /label=CB102_MBP-SnL_fw primer_bind complement(280..317) /label=CB453_A808P_rev primer_bind 297..331 /label=CB452_A808P_fwd primer_bind complement(370..394) /label=CB455_Q837P_rev primer_bind 375..420 /label=CB044 primer_bind complement(375..391) /label=CB143_MBP-SnC_rev primer_bind complement(378..407) /label=CB459_PD838G_rev primer_bind complement(381..420) /label=CB479_D838G_I839V_rev ORIGIN 1 atgggcagca gccatcatca tcatcatcac agcagcggcg gcctgaacga tatcttcgag 61 gcccagaaga tcgagtggca cgagggtagc ggtgggtctg gtagtggtgt gaataagaac 121 gataaaaagc cgctgcgtgg tgccgtgttt agcctgcaga aacagcatcc cgactatccc 181 gatatctatg gcgcgattga tcagaatggg acctatcaaa atgtgcgtac cggcgaagat 241 ggtaaactga cctttaagaa tctgagcgat ggcaaatatc gcctgtttga aaatagcgaa 301 cccccgggct ataaaccggt gcagaataag ccgattgtgg cgtttcagat tgtgaatggc 361 gaagtgcgtg atgtgaccag cattgtgccg ccgggtgtgc cggctacata tgaatttacc 421 taa //