LOCUS       Exported                 423 bp ds-DNA     circular SYN 11-NOV-2017
DEFINITION  clone 4 correct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Can Buldun
  TITLE     Direct Submission
  JOURNAL   Exported Nov 11, 2017 from SnapGene 4.0.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis affinity tag
                     /translation="HHHHHH"
     primer_bind     complement(22..75)
                     /label=CB033_AviIns_Rev
     CDS             40..84
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     complement(69..98)
                     /label=CB403_202-2_rev
     primer_bind     complement(81..111)
                     /label=CB491_AP+6SnL_rev
     primer_bind     91..130
                     /label=CB490_AP+6SnL_fwd
     primer_bind     99..136
                     /label=CB413_198_fwd
     primer_bind     106..142
                     /label=CB269_+6SnL_fwd
     CDS             109..420
                     /codon_start=1
                     /label=SnoopLigase
                     /translation="VNKNDKKPLRGAVFSLQKQHPDYPDIYGAIDQNGTYQNVRTGEDG
                     KLTFKNLSDGKYRLFENSEPPGYKPVQNKPIVAFQIVNGEVRDVTSIVPPGVPATYEFT
                     "
     primer_bind     127..142
                     /label=CB102_MBP-SnL_fw
     primer_bind     complement(280..317)
                     /label=CB453_A808P_rev
     primer_bind     297..331
                     /label=CB452_A808P_fwd
     primer_bind     complement(370..394)
                     /label=CB455_Q837P_rev
     primer_bind     375..420
                     /label=CB044
     primer_bind     complement(375..391)
                     /label=CB143_MBP-SnC_rev
     primer_bind     complement(378..407)
                     /label=CB459_PD838G_rev
     primer_bind     complement(381..420)
                     /label=CB479_D838G_I839V_rev
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcg gcctgaacga tatcttcgag
       61 gcccagaaga tcgagtggca cgagggtagc ggtgggtctg gtagtggtgt gaataagaac
      121 gataaaaagc cgctgcgtgg tgccgtgttt agcctgcaga aacagcatcc cgactatccc
      181 gatatctatg gcgcgattga tcagaatggg acctatcaaa atgtgcgtac cggcgaagat
      241 ggtaaactga cctttaagaa tctgagcgat ggcaaatatc gcctgtttga aaatagcgaa
      301 cccccgggct ataaaccggt gcagaataag ccgattgtgg cgtttcagat tgtgaatggc
      361 gaagtgcgtg atgtgaccag cattgtgccg ccgggtgtgc cggctacata tgaatttacc
      421 taa
//
