LOCUS       Exported                1263 bp ds-DNA     circular SYN 11-NOV-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Can Buldun
  TITLE     Direct Submission
  JOURNAL   Exported Nov 11, 2017 from SnapGene 4.0.6
            http://www.snapgene.com
COMMENT     clone 1 correct
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis affinity tag
                     /translation="HHHHHH"
     primer_bind     complement(19..47)
                     /label=CB599_343-1_rev
     primer_bind     complement(22..40)
                     /label=CB033_AviIns_Rev
     primer_bind     30..64
                     /label=CB598_343-1_fwd
     CDS             40..921
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag7
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEIS"
     primer_bind     complement(901..935)
                     /label=CB601_343-2_rev
     primer_bind     920..970
                     /label=CB600_343-2_fwd
     primer_bind     931..970
                     /label=CB490_AP+6SnL_fwd
     primer_bind     939..976
                     /label=CB413_198_fwd
     primer_bind     946..982
                     /label=CB269_+6SnL_fwd
     CDS             949..1260
                     /codon_start=1
                     /label=SnoopLigase
                     /translation="VNKNDKKPLRGAVFSLQKQHPDYPDIYGAIDQNGTYQNVRTGEDG
                     KLTFKNLSDGKYRLFENSEPPGYKPVQNKPIVAFQIVNGEVRDVTSIVPPGVPATYEFT
                     "
     primer_bind     967..982
                     /label=CB102_MBP-SnL_fw
     primer_bind     complement(1120..1157)
                     /label=CB453_A808P_rev
     primer_bind     1137..1171
                     /label=CB452_A808P_fwd
     primer_bind     complement(1210..1234)
                     /label=CB455_Q837P_rev
     primer_bind     1215..1260
                     /label=CB044
     primer_bind     complement(1215..1231)
                     /label=CB143_MBP-SnC_rev
     primer_bind     complement(1218..1247)
                     /label=CB459_PD838G_rev
     primer_bind     complement(1221..1260)
                     /label=CB479_D838G_I839V_rev
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcg aaatcggtac tggctttcca
       61 ttcgaccccc attatgtgga agtcctgggc gagcgcatgc actacgtcga tgttggtccg
      121 cgcgatggca cccctgtgct gttcctgcac ggtaacccga cctcctccta cgtgtggcgc
      181 aacatcatcc cgcatgttgc accgacccat cgctgcattg ctccagacct gatcggtatg
      241 ggcaaatccg acaaaccaga cctgggttat ttcttcgacg accacgtccg cttcatggat
      301 gccttcatcg aagccctggg tctggaagag gtcgtcctgg tcattcacga ctggggctcc
      361 gctctgggtt tccactgggc caagcgcaat ccagagcgcg tcaaaggtat tgcatttatg
      421 gagttcatcc gccctatccc gacctgggac gaatggccag aatttgcccg cgagaccttc
      481 caggccttcc gcaccaccga cgtcggccgc aagctgatca tcgatcagaa cgtttttatc
      541 gagggtacgc tgccgatggg tgtcgtccgc ccgctgactg aagtcgagat ggaccattac
      601 cgcgagccgt tcctgaatcc tgttgaccgc gagccactgt ggcgcttccc aaacgagctg
      661 ccaatcgccg gtgagccagc gaacatcgtc gcgctggtcg aagaatacat ggactggctg
      721 caccagtccc ctgtcccgaa gctgctgttc tggggcaccc caggcgttct gatcccaccg
      781 gccgaagccg ctcgcctggc caaaagcctg cctaactgca aggctgtgga catcggcccg
      841 ggtctgaatc tgctgcaaga agacaacccg gacctgatcg gcagcgagat cgcgcgctgg
      901 ctgtctactc tggagatttc cggtggtagc ggtgggtctg gtagtggtgt gaataagaac
      961 gataaaaagc cgctgcgtgg tgccgtgttt agcctgcaga aacagcatcc cgactatccc
     1021 gatatctatg gcgcgattga tcagaatggg acctatcaaa atgtgcgtac cggcgaagat
     1081 ggtaaactga cctttaagaa tctgagcgat ggcaaatatc gcctgtttga aaatagcgaa
     1141 cccccgggct ataaaccggt gcagaataag ccgattgtgg cgtttcagat tgtgaatggc
     1201 gaagtgcgtg atgtgaccag cattgtgccg ccgggtgtgc cggctacata tgaatttacc
     1261 taa
//
