LOCUS Exported 1215 bp ds-DNA circular SYN 11-NOV-2017 DEFINITION clone 2 correct. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1215) AUTHORS Can Buldun TITLE Direct Submission JOURNAL Exported Nov 11, 2017 from SnapGene 4.0.6 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1215 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 13..30 /codon_start=1 /product="6xHis affinity tag" /label=6xHis affinity tag /translation="HHHHHH" primer_bind complement(33..63) /label=CB569_328_rev CDS 40..75 /codon_start=1 /label=SnoopTagJr /translation="KLGSIEFIKVNK" primer_bind 43..82 /label=CB568_328_fwd primer_bind 52..116 /label=CB345_171_fwd CDS 103..1200 /codon_start=1 /gene="malE (mutated)" /product="maltose binding protein from E. coli" /label=MBP /note="This version of the gene does not encode a signal sequence, so MBP will remain in the cytosol." /translation="KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE NYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFW YAVRTAVINAASGRQTVDEALKDAQT" ORIGIN 1 atgggcagca gccatcacca ccatcatcac agcagcggca aactgggcag catcgaattc 61 atcaaagtga acaaaggtag tggtgaaagt ggtgggtcag ggaaaatcga agaaggtaaa 121 ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa 181 ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa 241 ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc 301 tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag 361 gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac 421 ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca 481 aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg 541 ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt 601 tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct 661 ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca 721 gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc 781 aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta 841 ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt 901 aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact 961 gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct 1021 tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa 1081 ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg 1141 gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact 1201 aattcgagct cgtaa //