LOCUS       Exported                 402 bp ds-DNA     circular SYN 11-NOV-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Can Buldun
  TITLE     Direct Submission
  JOURNAL   Exported Nov 11, 2017 from SnapGene 4.0.6
            http://www.snapgene.com
COMMENT     clone 1 correct
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     4..34
                     /label=CB253_WSUMO_fwd
     CDS             16..33
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis affinity tag
                     /translation="HHHHHH"
     CDS             34..327
                     /codon_start=1
                     /label=SUMO
                     /translation="GSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     primer_bind     complement(312..357)
                     /label=CB098_SUMOSnT2_fwd
     primer_bind     complement(329..377)
                     /label=CB443_N847D_rev
     CDS             331..399
                     /codon_start=1
                     /label=DogTag
                     /translation="DIPATYEFTDGKHYITNEPIPPK"
     primer_bind     complement(331..387)
                     /label=CB097_SnT2_rev
     primer_bind     331..387
                     /label=CB096_SnT2_fwd
     primer_bind     complement(331..377)
                     /label=CB705_N847D_rev
     primer_bind     341..392
                     /label=CB442_N847D_fwd
     primer_bind     complement(361..402)
                     /label=CB121_SnT2+4_rev
ORIGIN
        1 atgggtagct ggagccatca ccatcatcat cacgggagcg atagcgaagt gaaccaggaa
       61 gcgaaaccgg aagttaaacc ggaagtgaaa ccggaaaccc atattaatct gaaagttagc
      121 gacggcagca gcgaaatctt ttttaaaatt aaaaaaacca ccccgctgcg tcgcctgatg
      181 gaagcctttg cgaaacgtca gggtaaagaa atggatagcc tgcgctttct gtatgacggc
      241 atccgtattc aggccgatca gaccccggaa gacctggata tggaagacaa cgatattatt
      301 gaagcgcatc gcgaacagat cggtggtggc gatattccgg ctacatatga atttaccgat
      361 ggtaaacatt atatcaccaa tgaaccgata ccgccgaaat aa
//
