LOCUS       Exported                7211 bp ds-DNA     circular SYN 22-DEC-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    D401_pAAV-CKIIa-GtACR2-ts-FRed-Kv2.1.dna
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   Tivan27
  TITLE     Direct Submission
  JOURNAL   Exported Dec 22, 2017 from SnapGene 4.0.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7211
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     primer_bind     65..82
                     /label=OS22
     primer_bind     114..135
                     /label=OS21
     primer_bind     126..145
                     /label=OS26
     primer_bind     137..151
                     /label=MM154_ITR-MluI_fw_seq
     promoter        156..1448
                     /label=CaMKIIa(1.3)
                     /note="Neocortex pyramidal neuron specific promoter"
     primer_bind     249..268
                     /label=S016
     primer_bind     249..268
                     /label=S16_seq CaMKII 1
     primer_bind     complement(396..417)
                     /label=OS67
     primer_bind     909..928
                     /label=S017
     primer_bind     909..928
                     /label=S17_seq CaMKII 2
     primer_bind     1086..1111
                     /label=C114_CKIIa(0.4)-fw
     primer_bind     1086..1111
                     /label=C114
     primer_bind     1086..1111
                     /label=MM137_MluI-Nhe-CKIIa_fw
     primer_bind     1086..1111
                     /label=MM147_MluI-Nhe-CKIIa_fw
     primer_bind     1313..1331
                     /label=S004
     primer_bind     complement(1431..1478)
                     /label=MM185_AgeI-CKII_mScarlet_rev
     primer_bind     complement(1457..1477)
                     /label=BeGC_trun_rev_(454)
     primer_bind     complement(1457..1477)
                     /label=MM68_Proj2_FS-Fix_Rev
     primer_bind     1462..1487
                     /label=BamHI_GtACR1_for_(481)
     primer_bind     1467..1486
                     /label=NheI_GtACR1_for_(540)
     primer_bind     1473..1498
                     /label=p2a_GtACR2_for_2_(622)
     CDS             1475..2347
                     /codon_start=1
                     /label=GtACR2
                     /translation="MASQVVYGEWASTHTECYNMSRIDSTFVSLLQLVWAVVSGCQTIF
                     MISRAPKVPWESVYLPFVESITYALASTGNGTLQMRDGRFFPWSRMASWLCTCPIMLGQ
                     ISNMALVKYKSIPLNPIAQAASIIRVVMGITATISPAEYMKWLFFFFGATCLVFEYSVV
                     FTIFQVGLYGFESVGTPLAQKVVVRIKMLRLIFFIAWTMFPIVWLISPTGVCVIHENVS
                     AILYLLADGLCKNTYGVILWSTAWGVLEGKWDPACLPGQEKPEADDPFGLNHEKNAPPN
                     DEVNIRMFGR"
     primer_bind     1475..1499
                     /label=GtACR2_o_beta_for_(571)
     primer_bind     complement(1749..1786)
                     /label=GtACR2_C98A_for_(501_1)
     primer_bind     1749..1786
                     /label=GtACR2_C98A_for_(500_1)
     primer_bind     1751..1782
                     /label=QC_GtACR1_C98A_rev_(501)
     primer_bind     complement(1751..1782)
                     /label=QC_GtACR1_C98A_for_(500)
     primer_bind     1754..1795
                     /label=QC_GtACR2_C98A_for_(546)
     primer_bind     1833..1864
                     /label=QC_GtACR1_S126A_rev_(503)
     primer_bind     complement(1833..1864)
                     /label=QC_GtACR1_S126A_for_(502)
     primer_bind     complement(2318..2347)
                     /label=Mfe_GtACR2_rev_(572)
     primer_bind     complement(2320..2374)
                     /label=MM97_GtACR2-ts_fusion
     primer_bind     complement(2332..2348)
                     /label=MM186_GtACR2-FRed_rev
     primer_bind     complement(2334..2348)
                     /label=AgeI_GtACR2_rev_(482)
     primer_bind     2347..2366
                     /label=YP26_TS_fwd
     primer_bind     2348..2375
                     /label=MM98_AA-ts_for
     CDS             2354..2413
                     /codon_start=1
                     /product="membrane trafficking sequence Kir2.1"
                     /label=ts
                     /translation="KSRITSEGEYIPLDQIDINV"
     primer_bind     2354..2370
                     /label=MM167-ts-Fwd
     primer_bind     2356..2374
                     /label=seq_ts_for_(391)
                     /note="0.025,DST,19"
     primer_bind     2365..2374
                     /label=BeGC1_H564R_V568F_for_(475)
                     /note="0.025,DST,"
     primer_bind     2371..2443
                     /label=ts_FR_for_(424)
                     /note="0.05,DST,73"
     primer_bind     complement(2390..2416)
                     /label=P2A_C1V1_rev_(225) (2)
                     /note="93,,"
     primer_bind     complement(2390..2416)
                     /label=P2A_C1V1_rev_(225)
                     /note="93,,"
     primer_bind     complement(2390..2414)
                     /label=OC92
     primer_bind     complement(2393..2416)
                     /label=MM120_ts_rev_seq
     primer_bind     complement(2395..2428)
                     /label=YP27_TS_rev
     primer_bind     complement(2401..2416)
                     /label=ts_rev_(372)
                     /note="0.025,DST,"
     primer_bind     complement(2407..2426)
                     /label=FP635_for_(459)
                     /note="0.025,DST,"
     primer_bind     2416..2437
                     /label=VVD_o_mKate2_for_(31)
                     /note="63,,"
     CDS             2417..3112
                     /codon_start=1
                     /label=FusionRed
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     primer_bind     2417..2443
                     /label=AscI_FR_for_(257)
                     /note="48,,"
     primer_bind     2417..2440
                     /label=MM187_Fred_fw
     primer_bind     2417..2440
                     /label=o_T2A_BeGC1_for_(520)
     primer_bind     2417..2440
                     /label=Nhe_FRed_for_(353)
                     /note="0.025,DST,"
     primer_bind     2417..2440
                     /label=Fred_for_(388)
                     /note="0.025,DST,35"
     primer_bind     2417..2440
                     /label=BamHI_FR_for_(351)
                     /note="0.025,DST,"
     primer_bind     2417..2440
                     /label=Primer 1
     primer_bind     2417..2438
                     /label=a767c_t769g_(335)
     primer_bind     2417..2437
                     /label=PDFG_mKate_for_(18)
                     /note="71,,"
     primer_bind     2417..2428
                     /label=FRed_for_(381)
                     /note="0.025,DST,21"
     primer_bind     2418..2443
                     /label=FRed_o_CoChR2_(574)
     primer_bind     2421..2446
                     /label=MM91_TagBFP-FW
     primer_bind     2422..2438
                     /label=CoChR_STOP290G_rev_(581)
     primer_bind     complement(2464..2479)
                     /label=OS14
     primer_bind     complement(2482..2504)
                     /label=seq_TurboBFP_rev_(346)_(246)
                     /note="0.025,DST,"
     primer_bind     2561..2570
                     /label=EcoRI_RFP_rev_(198)
                     /note="45,DST,"
     primer_bind     2561..2570
                     /label=AscI_mRFP_rev_(251)
                     /note="47,,"
     primer_bind     2575..2587
                     /label=ChR2_o_PR_r_(113)
                     /note="54,,"
     primer_bind     2575..2587
                     /label=ChR2_o_P2A_r_(112)
                     /note="57,,"
     primer_bind     complement(2579..2595)
                     /label=seq_mRuby_rev_(600)
     primer_bind     complement(2731..2750)
                     /label=YP8_seq2
     primer_bind     2918..2935
                     /label=lox_o_mKate(168)_for_(154)
                     /note="51,,"
     primer_bind     complement(3050..3068)
                     /label=seq_Fp635-C_For_(376)
                     /note="0.025,DST,"
     primer_bind     3050..3068
                     /label=seq_Fp635-C_For_(375)
                     /note="0.025,DST,"
     primer_bind     complement(3089..3112)
                     /label=TG_AgeI_FR_rev_(258)
                     /note="63,,"
     primer_bind     3090..3140
                     /label=FRed-Kv2.1-fusion
     primer_bind     complement(3090..3112)
                     /label=FRed_o_beta_rev_(575)
     primer_bind     complement(3092..3112)
                     /label=Fred_rev_(382)
     primer_bind     complement(3092..3112)
                     /label=Mfe_FRed_rev_(389)
     primer_bind     complement(3092..3112)
                     /label=Fragment.REV_(337)
     primer_bind     complement(3092..3112)
                     /label=er_FRed_rev_(478)
     primer_bind     complement(3092..3112)
                     /label=EcoRI_ER_FR_rev_(421)
     primer_bind     complement(3093..3113)
                     /label=BsiWI_FR_rev_(352)
     primer_bind     complement(3093..3112)
                     /label=EcoRI_FRed_rev_(354)
     primer_bind     complement(3093..3112)
                     /label=AscI_FRed_rev_(561)
     primer_bind     complement(3095..3112)
                     /label=FRed_STOP_rev_(583)
     CDS             3113..3307
                     /codon_start=1
                     /product="A Novel Targeting Signal for Proximal Clustering 
                     of the Kv2.1 K+ Channel in Hippocampal Neurons
                     Lim et al., Neuron 2000
                     http://www.sciencedirect.com/science/article/pii/S089662730
                     0809022"
                     /label=Kv2.1 targeting domain
                     /translation="QSQPILNTKEMAPQSKPPEELEMSSMPSPVAPLPARTEGVIDMRS
                     MSSIDSFISCATDFPEATRF"
     primer_bind     3113..3136
                     /label=YP1_CoChR-Kv2.1
     primer_bind     3113..3136
                     /label=YP34_ER-Kv
     primer_bind     3224..3244
                     /label=YP32_Kvmin_fwd
     primer_bind     3224..3244
                     /label=YP3_CoChR-Kv2.1min
     primer_bind     complement(3242..3274)
                     /label=YP5_Kv2.1
     primer_bind     complement(3279..3301)
                     /label=YP33_Kvmin_rev
     primer_bind     complement(3279..3301)
                     /label=YP25_Kvmin
     primer_bind     complement(3279..3301)
                     /label=YP4_p2A-Kv2.1min
     primer_bind     complement(3284..3310)
                     /label=MM165_GCN4_v4_Kv2.1_rev
     primer_bind     complement(3286..3307)
                     /label=YP2_p2A-Kv2.1
     primer_bind     complement(3288..3307)
                     /label=YP22_Kv_rev
     primer_bind     complement(3290..3310)
                     /label=MM158_Eco-Pest-Kv2.1-Rev_1
     primer_bind     complement(3290..3307)
                     /label=YP31_Kv_rev
     primer_bind     3314..3343
                     /label=QC_desEcoRI_rev_(471)
     primer_bind     complement(3319..3350)
                     /label=C101_WPRE-Rv
     primer_bind     complement(3319..3350)
                     /label=C101
     primer_bind     complement(3325..3350)
                     /label=Double LOX 3' SypHy primer creation- R'
     primer_bind     3326..3349
                     /label=pL_hSyn_for_(159)
     primer_bind     3332..3365
                     /label=MM148_MluI-EcoRI-WPRE
     misc_feature    3338..3926
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     3338..3373
                     /label=MM146_WPRE_fw
     primer_bind     complement(3392..3413)
                     /label=OS07
     primer_bind     3411..3432
                     /label=OS68
     primer_bind     complement(3413..3432)
                     /label=G35 Ai32-mut-rev
     primer_bind     complement(3413..3432)
                     /label=G06 Ai9 mut R'
     primer_bind     3605..3624
                     /label=SqP for pCL-CAG-EGFP-WPRE_11
     primer_bind     3745..3764
                     /label=OS46
     primer_bind     3775..3796
                     /label=OS33
     CDS             complement(3809..3820)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3863..3890
                     /label=OC63
     primer_bind     3896..3915
                     /label=SqP for pCL-CAG-EGFP-WPRE_12
     polyA_signal    3958..4434
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3962..3982)
                     /label=OS70(missing)
     primer_bind     complement(4464..4479)
                     /label=MM153_ITR_rev_seq
     primer_bind     complement(4470..4489)
                     /label=OS26
     repeat_region   4474..4614
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     primer_bind     complement(4480..4501)
                     /label=OS21
     primer_bind     complement(4533..4550)
                     /label=OS22
     rep_origin      4689..5144
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4695..4714)
                     /label=MM119_Sequencing_ITR2
     primer_bind     complement(5334..5353)
                     /label=SqP for pRS315 (LEU)_01
     primer_bind     complement(5340..5359)
                     /label=SqP for pRS315 (LEU)_11
     promoter        5426..5530
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5426..5445)
                     /label=SqP for pRS315 (LEU)_10
     CDS             5531..6391
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6027..6046)
                     /label=SqP for pRS315 (LEU)_09
     rep_origin      6562..7150
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6610..6624
                     /label=SecSig_o_Bgtx_for_(245)
     primer_bind     complement(6628..6647)
                     /label=SqP for pRS315 (LEU)_08
     primer_bind     7087..7106
                     /label=MM118_Sequencing_ITR1
     primer_bind     7131..7154
                     /label=OC57a
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtttaac attatggcct taggtcactt
      181 catctccatg gggttcttct tctgattttc tagaaaatga gatgggggtg cagagagctt
      241 cctcagtgac ctgcccaggg tcacatcaga aatgtcagag ctagaacttg aactcagatt
      301 actaatctta aattccatgc cttgggggca tgcaagtacg atatacagaa ggagtgaact
      361 cattagggca gatgaccaat gagtttagga aagaagagtc cagggcaggg tacatctaca
      421 ccacccgccc agccctgggt gagtccagcc acgttcacct cattatagtt gcctctctcc
      481 agtcctacct tgacgggaag cacaagcaga aactgggaca ggagccccag gagaccaaat
      541 cttcatggtc cctctgggag gatgggtggg gagagctgtg gcagaggcct caggaggggc
      601 cctgctgctc agtggtgaca gataggggtg agaaagcaga cagagtcatt ccgtcagcat
      661 tctgggtctg tttggtactt cttctcacgc taaggtggcg gtgtgatatg cacaatggct
      721 aaaaagcagg gagagctgga aagaaacaag gacagagaca gaggccaagt caaccagacc
      781 aattcccaga ggaagcaaag aaaccattac agagactaca agggggaagg gaaggagaga
      841 tgaattagct tcccctgtaa accttagaac ccagctgttg ccagggcaac ggggcaatac
      901 ctgtctcttc agaggagatg aagttgccag ggtaactaca tcctgtcttt ctcaaggacc
      961 atcccagaat gtggcaccca ctagccgtta ccatagcaac tgcctctttg ccccacttaa
     1021 tcccatcccg tctgttaaaa gggccctata gttggaggtg ggggaggtag gaagagcgat
     1081 gatcacttgt ggactaagtt tgttcgcatc cccttctcca accccctcag tacatcaccc
     1141 tgggggaaca gggtccactt gctcctgggc ccacacagtc ctgcagtatt gtgtatataa
     1201 ggccagggca aagaggagca ggttttaaag tgaaaggcag gcaggtgttg gggaggcagt
     1261 taccggggca acgggaacag ggcgtttcgg aggtggttgc catggggacc tggatgctga
     1321 cgaaggctcg cgaggctgtg agcagccaca gtgccctgct cagaagcccc aagctcgtca
     1381 gtcaagccgg ttctccgttt gcactcagga gcacgggcag gcgagtggcc cctagttctg
     1441 ggggcagctc tagagcggta ccggatccgc caccatggca tcacaggtcg tctatgggga
     1501 atgggcatca actcacaccg agtgctacaa tatgtccaga atcgactcca ccttcgtctc
     1561 actgctgcag ctggtgtggg ccgtggtctc aggctgccag acaatcttca tgattagcag
     1621 ggctcccaag gtcccttggg agtccgtgta cctgcccttt gtcgaatcta tcacttatgc
     1681 actggccagt actggcaacg gaaccctgca gatgagggac ggcagattct ttccttggag
     1741 ccgaatggcc tcctggctgt gcacctgtcc aatcatgctg ggacagattt ctaacatggc
     1801 tctggtgaag tacaaaagta tcccactgaa tcccattgca caggccgctt ccatcattag
     1861 agtggtcatg ggaatcaccg ctacaatttc tcccgcagag tatatgaagt ggctgttctt
     1921 tttctttggg gccacctgcc tggtgttcga gtacagtgtg gtcttcacaa tctttcaagt
     1981 ggggctgtat ggctttgaat ctgtcgggac acctctggca cagaaggtgg tggtgaggat
     2041 caagatgctg cgcctgatct tctttattgc ttggactatg ttcccaatcg tgtggctgat
     2101 ttcacccacc ggagtctgcg tgatccacga gaacgtgagc gccattctgt acctgctggc
     2161 tgacggcctg tgcaaaaata catatggagt gatcctgtgg agcactgcct ggggagtgct
     2221 ggaagggaag tgggaccccg catgtctgcc agggcaggag aaacccgaag ccgacgatcc
     2281 ttttggcctg aatcacgaga agaatgcccc tcctaacgac gaagtcaata tcagaatgtt
     2341 tggaagggcc gccaagagca ggatcaccag cgagggcgag tacatccccc tggaccagat
     2401 cgacatcaac gtggtgatgg tgagcgagct gattaaggag aacatgccca tgaagctgta
     2461 catggagggc accgtgaaca accaccactt caagtgcaca tccgagggcg aaggcaagcc
     2521 ctacgagggc acccagacca tgagaatcaa ggtcgtcgag ggcggccctc tccccttcgc
     2581 cttcgacatc ctggctacca gcttcatgta cggcagcaga accttcatca agcaccctcc
     2641 gggcatcccc gacttcttta agcagtcctt ccctgagggc ttcacatggg agagagtcac
     2701 cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg
     2761 cctcatctac aacgtcaagg ttagaggggt gaacttccca gccaacggcc ctgtgatgca
     2821 gaagaaaaca ctcggctggg aggcctccac cgagacgatg taccctgctg acggaggact
     2881 ggaaggcgca tgtgacatgg ctctgaagct cgtgggagga ggacacctga tctgcaacct
     2941 tgagacaaca tacagatcca agaaacccgc tacgaacctc aagatgcccg gcgtctacaa
     3001 cgtggaccac agactggaaa gaatcaagga ggccgacgat gagacatacg tcgagcagca
     3061 cgaggtggct gtggccagat actctactgg tggcgctggt gatggaggta aacagtccca
     3121 gcctattctg aacaccaaag agatggcccc ccagagtaaa ccccctgagg agctggaaat
     3181 gtcctcaatg ccctcccctg tggcacctct tcctgcaaga acagagggcg tgatcgacat
     3241 gaggtccatg agttctattg actcttttat ctcatgcgcc accgattttc cagaggctac
     3301 gcggtttaag taagaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     3361 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     3421 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     3481 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     3541 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     3601 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     3661 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     3721 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     3781 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     3841 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     3901 gatctccctt tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga gatctacggg
     3961 tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg
     4021 cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc
     4081 tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc
     4141 tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc
     4201 actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg
     4261 ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt
     4321 ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg
     4381 cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt
     4441 ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca
     4501 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
     4561 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
     4621 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
     4681 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4741 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4801 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4861 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
     4921 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4981 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt
     5041 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     5101 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
     5161 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     5221 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     5281 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     5341 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     5401 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5461 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5521 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5581 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5641 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     5701 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     5761 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     5821 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     5881 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5941 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     6001 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     6061 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     6121 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     6181 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     6241 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     6301 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     6361 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     6421 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6481 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6541 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6601 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6661 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     6721 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6781 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6841 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6901 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6961 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     7021 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7081 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     7141 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     7201 tgctcacatg t
//
