LOCUS       Exported                6457 bp ds-DNA     circular SYN 22-DEC-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    D435_pAAV-hSyn-SIO-GtACR2-TS-FRed-Kv2.1.dna
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Tivan27
  TITLE     Direct Submission
  JOURNAL   Exported Dec 22, 2017 from SnapGene 4.0.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     promoter        165..633
                     /label=hSyn1
     primer_bind     575..593
                     /label=OS08
                     /note="Sequences forward from CamKII promoter"
     protein_bind    647..680
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="loxP mutant. Cre-mediated recombination occurs in 
                     the 8-bp core sequence (GCATACAT)."
     CDS             complement(687..887)
                     /codon_start=1
                     /label=Kv2.1 targeting domain
                     /note="color: #993366"
                     /translation="QSQPILNTKEMAPQSKPPEELEMSSMPSPVAPLPARTEGVIDMRS
                     MSSIDSFISCATDFPEATRFK"
     primer_bind     complement(860..910)
                     /label=MM101_FRed-Kv2.1-fusion
     primer_bind     complement(860..910)
                     /label=FRed-Kv2.1-fusion
     CDS             complement(888..1583)
                     /codon_start=1
                     /label=FusionRed
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     CDS             complement(1584..1646)
                     /codon_start=1
                     /label=ts
                     /translation="KSRITSEGEYIPLDQIDINVV"
     CDS             complement(1653..2525)
                     /codon_start=1
                     /label=GtACR2
                     /translation="MASQVVYGEWASTHTECYNMSRIDSTFVSLLQLVWAVVSGCQTIF
                     MISRAPKVPWESVYLPFVESITYALASTGNGTLQMRDGRFFPWSRMASWLCTCPIMLGQ
                     ISNMALVKYKSIPLNPIAQAASIIRVVMGITATISPAEYMKWLFFFFGATCLVFEYSVV
                     FTIFQVGLYGFESVGTPLAQKVVVRIKMLRLIFFIAWTMFPIVWLISPTGVCVIHENVS
                     AILYLLADGLCKNTYGVILWSTAWGVLEGKWDPACLPGQEKPEADDPFGLNHEKNAPPN
                     DEVNIRMFGR"
     primer_bind     complement(2513..2537)
                     /label=BamHI_GtACR1_for_(481)
     protein_bind    complement(2538..2571)
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="loxP mutant. Cre-mediated recombination occurs in 
                     the 8-bp core sequence (GCATACAT)."
     misc_feature    2584..3172
                     /label=WPRE
                     /note="woodchuck posttranscriptional regulatory"
     primer_bind     complement(2638..2659)
                     /label=OS07
                     /note="Sequences back from WPRE on Lenti vectors"
     CDS             complement(3055..3066)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3204..3680
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal color: 
                     #a6acb3"
     repeat_region   3720..3860
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3935..4390
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis color: #ffff00"
     promoter        4672..4776
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             4777..5637
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics This feature has 2 segments:    1: 5567
                     ..   12 / #ccffcc / signal sequence    2:   13 ..  804 / 
                     #ccffcc Cleavage site after base 12"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5808..6396
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication color: #ffff00"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc
      181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg
      241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta
      301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca
      361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa
      421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc
      481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
      601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtacctacc gttcgtatag
      661 catacattat acgaagttat gaattcttac ttaaaccgcg tagcctctgg aaaatcggtg
      721 gcgcatgaga taaaagagtc aatagaactc atggacctca tgtcgatcac gccctctgtt
      781 cttgcaggaa gaggtgccac aggggagggc attgaggaca tttccagctc ctcagggggt
      841 ttactctggg gggccatctc tttggtgttc agaataggct gggactgttt acctccatca
      901 ccagcgccac cagtagagta tctggccaca gccacctcgt gctgctcgac gtatgtctca
      961 tcgtcggcct ccttgattct ttccagtctg tggtccacgt tgtagacgcc gggcatcttg
     1021 aggttcgtag cgggtttctt ggatctgtat gttgtctcaa ggttgcagat caggtgtcct
     1081 cctcccacga gcttcagagc catgtcacat gcgccttcca gtcctccgtc agcagggtac
     1141 atcgtctcgg tggaggcctc ccagccgagt gttttcttct gcatcacagg gccgttggct
     1201 gggaagttca cccctctaac cttgacgttg tagatgaggc agccgtcctg gaggctggtg
     1261 tcctgggtag cggtcagcac gcccccgtct tcgtatgtgg tgactctctc ccatgtgaag
     1321 ccctcaggga aggactgctt aaagaagtcg gggatgcccg gagggtgctt gatgaaggtt
     1381 ctgctgccgt acatgaagct ggtagccagg atgtcgaagg cgaaggggag agggccgccc
     1441 tcgacgacct tgattctcat ggtctgggtg ccctcgtagg gcttgccttc gccctcggat
     1501 gtgcacttga agtggtggtt gttcacggtg ccctccatgt acagcttcat gggcatgttc
     1561 tccttaatca gctcgctcac catcaccacg ttgatgtcga tctggtccag ggggatgtac
     1621 tcgccctcgc tggtgatcct gctcttggcg gcccttccaa acattctgat attgacttcg
     1681 tcgttaggag gggcattctt ctcgtgattc aggccaaaag gatcgtcggc ttcgggtttc
     1741 tcctgccctg gcagacatgc ggggtcccac ttcccttcca gcactcccca ggcagtgctc
     1801 cacaggatca ctccatatgt atttttgcac aggccgtcag ccagcaggta cagaatggcg
     1861 ctcacgttct cgtggatcac gcagactccg gtgggtgaaa tcagccacac gattgggaac
     1921 atagtccaag caataaagaa gatcaggcgc agcatcttga tcctcaccac caccttctgt
     1981 gccagaggtg tcccgacaga ttcaaagcca tacagcccca cttgaaagat tgtgaagacc
     2041 acactgtact cgaacaccag gcaggtggcc ccaaagaaaa agaacagcca cttcatatac
     2101 tctgcgggag aaattgtagc ggtgattccc atgaccactc taatgatgga agcggcctgt
     2161 gcaatgggat tcagtgggat acttttgtac ttcaccagag ccatgttaga aatctgtccc
     2221 agcatgattg gacaggtgca cagccaggag gccattcggc tccaaggaaa gaatctgccg
     2281 tccctcatct gcagggttcc gttgccagta ctggccagtg cataagtgat agattcgaca
     2341 aagggcaggt acacggactc ccaagggacc ttgggagccc tgctaatcat gaagattgtc
     2401 tggcagcctg agaccacggc ccacaccagc tgcagcagtg agacgaaggt ggagtcgatt
     2461 ctggacatat tgtagcactc ggtgtgagtt gatgcccatt ccccatagac gacctgtgat
     2521 gccatggtgg cggatcctac cgttcgtata atgtatgcta tacgaagtta taagcttatc
     2581 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     2641 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     2701 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     2761 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     2821 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     2881 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     2941 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     3001 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     3061 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     3121 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     3181 cgagcgctgc tcgagagatc tacgggtggc atccctgtga cccctcccca gtgcctctcc
     3241 tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa ttaagttgca
     3301 tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg gtggtatgga
     3361 gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg gaaccaagct
     3421 ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt caagcgattc
     3481 tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg ctcagctaat
     3541 ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc tccaactcct
     3601 aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag gcgtgaacca
     3661 ctgctccctt ccctgtcctt ctgattttgt aggtaaccac gtgcggaccg agcggccgca
     3721 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc
     3781 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
     3841 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     3901 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     3961 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4021 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4081 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4141 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4201 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     4261 ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4321 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     4381 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     4441 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     4501 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     4561 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     4621 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4681 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4741 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     4801 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     4861 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     4921 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     4981 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5041 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5101 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5161 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5221 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5281 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5341 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5401 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5461 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5521 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5581 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5641 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     5701 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     5761 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     5821 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     5881 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     5941 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6001 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6061 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6121 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6181 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6241 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6301 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6361 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6421 cggttcctgg ccttttgctg gccttttgct cacatgt
//
