LOCUS       pAAV-Ef1a-fDIO-GCaMP6s        6857 bp    DNA     circular     28-JUL-2017
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-11871063|
COMMENT     VNTDBDATE|-11871063|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV-Ef1a-fDIO-GCaMP6s|
COMMENT     VNTAUTHORNAME|Rylan Larsen|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAAV-Ef1a-fDIO-GCaMP6s" 0 6857 
COMMENT       (CDBMol 0 0 1 4022 1 0 0 0 1 "" "" 0 0 0 0 
COMMENT        (CObList 
COMMENT         #0=(CClstep 
COMMENT             (CStringList "AarI" "AsiSI" "AvrII" "BaeI" "BsaBI" "BsiWI" "BsmI" 
COMMENT              "BstZ17I" "Bsu36I" "FspAI" "M.BaeI" "M.EcoAI" "M.KpnAI" "M.KpnBI" 
COMMENT              "M.NgoAV" "M.PshAI" "M.SnaBI" "M.Tth111I" "M.XcmI" "NdeI" "NruI" 
COMMENT              "PacI" "PmeI" "PpuMI" "PshAI" "PsrI" "SanDI" "SexAI" "SfiI" 
COMMENT              "SgrAI" "SnaBI" "SpeI" "Sse232I" "Sse8647I" "SwaI" "Tth111I" 
COMMENT              "XcmI") 
COMMENT             (CStringList "AscI" "BmgBI" "HpaI" "M.EcoBI" "M.HpaI" "NcoI" "SapI") 
COMMENT             (CStringList "NheI" "AfeI" "AflII" "AgeI" "AlwNI" "ApaBI" "ApaI" 
COMMENT              "AseI" "BamHI" "BfrBI" "BmtI" "BspEI" "BstAPI" "BstEII" "EcoRI" 
COMMENT              "EcoRV" "FseI" "FspI" "M.ApaI" "M.BnaI" "M.BstI" "M.EcaI" 
COMMENT              "M.EcoKI" "M.EcoRI" "M.EcoRV" "M.MunI" "M.NmeSI" "M.PhiBssHII" 
COMMENT              "M.SsoI" "M.StyLTIII" "M.StySPI" "M.VspI" "MfeI" "MluI" "NsiI" 
COMMENT              "PflMI" "Ppu10I" "PsiI" "PspOMI" "PvuI" "RsrII" "ScaI" "SphI") 1 
COMMENT             "pAAV-Ef1a-fDIO-GCaMP6s" 0 "pAAV-Ef1a-fDIO hChR2(H134R)-EYFP" 
COMMENT             "GCaMP6s_RC_insert_forcloning" 0 "AscI" 1 1 0 0 0 0 "NheI" 1 1 0 0 
COMMENT             0 0 "AscI" 0 1 1 0 0 0 0 0 "NheI" 0 1 1 0 0 0 0 0 "" "" 0 "" "" 0 
COMMENT             "" 0 "" 0 0 0 0 0 0 0 2 0 2 "" "" 1 0 0 "" "" 1 1 0 1 0 0 "AscI" 
COMMENT             "NheI" 1 "BclI" "" "" "" "cgcgccttactacttcgctg" "" "")) (CObList) 
COMMENT        (CObList 
COMMENT         #1=(CFragment 3 1 0 1 0 "" 0 "" "" "" "" "" "" 
COMMENT             "pAAV-Ef1a-fDIO hChR2(H134R)-EYFP" "" 0 0 1 5590 2837 1569 3246 
COMMENT             1569 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "NheI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "AscI" "" 1 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #2=(CFragment 3 1 0 1 0 "" 8 "" "" "" "" "" "" 
COMMENT             "GCaMP6s_RC_insert_forcloning" "" 0 0 1 1267 1570 2836 3 1269 0 "" 
COMMENT             "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "AscI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "NheI" "" 1 0 0 0 0 0 0 0 0 0) 1 0)) 
COMMENT        (CObList 
COMMENT         #3=(CFragment 3 2 0 0 0 "" 0 "" "" "" "" "" "" 
COMMENT             "pAAV-Ef1a-fDIO hChR2(H134R)-EYFP" "" 0 0 1 5590 0 0 3246 1569 0 "" 
COMMENT             "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "NheI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "AscI" "" 1 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #4=(CFragment 3 2 0 0 0 "" 0 "" "" "" "" "" "" 
COMMENT             "GCaMP6s_RC_insert_forcloning" "" 0 0 1 0 0 0 0 0 0 "" "" "" "" "" 
COMMENT             "" 
COMMENT             (CObList 
COMMENT              #5=(CFragD 21 "2x stop" 9 14 1 
COMMENT                  "Misc. Feature, Name: 2x stop, From 9 To 14 (Complementary)") 
COMMENT              #6=(CFragD 21 "Kozak" 1263 1268 1 
COMMENT                  "Misc. Feature, Name: Kozak, From 1263 To 1268 (Complementary)")) 
COMMENT             (CTerm 1 0 0 "AscI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "NheI" "" 1 0 0 0 0 0 0 0 0 0) 1 0)) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) (CStringList "SmaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 6857 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #7=(CRSite (CStringList) "SmaI" "cccggg" 4 0 4 43 0 54 0 4208 0 4219 0 0 
COMMENT            "")) 
COMMENT       (CObList 
COMMENT        #8=(CFSignal (CObList) "pBR322ori-F" 28 0 0 6697 6716 1 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "7106..7125") 
COMMENT        #9=(CFSignal (CObList) "ori" 33 0 0 6208 6796 1 (CStringList) 
COMMENT            (CStringList "/direction=RIGHT") 1 1 1 1 "6617..7205") 
COMMENT        #10=(CFSignal (CObList) "Amp-R" 28 0 1 5395 5414 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5804..5823)") 
COMMENT        #11=(CFSignal (CObList) "AmpR" 4 0 0 5177 6037 1 (CStringList) 
COMMENT             (CStringList "/codon_start=1" "/gene=\"bla\"" 
COMMENT              "/product=\"beta-lactamase\"") 1 1 1 1 "5586..6446") 
COMMENT        #12=(CFSignal (CObList) "AmpR promoter" 30 0 0 5072 5176 1 (CStringList) 
COMMENT             (CStringList "/gene=\"bla\"") 1 1 1 1 "5481..5585") 
COMMENT        #13=(CFSignal (CObList) "pBRforEco" 28 0 1 4986 5004 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5395..5413)") 
COMMENT        #14=(CFSignal (CObList) "pGEX 3'" 28 0 0 4926 4948 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5335..5357") 
COMMENT        #15=(CFSignal (CObList) "pRS-marker" 28 0 1 4807 4826 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5216..5235)") 
COMMENT        #16=(CFSignal (CObList) "F1ori-F" 28 0 0 4632 4653 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5041..5062") 
COMMENT        #17=(CFSignal (CObList) "F1ori-R" 28 0 1 4422 4441 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4831..4850)") 
COMMENT        #18=(CFSignal (CObList) "f1 ori" 33 0 0 4335 4790 1 (CStringList) 
COMMENT             (CStringList "/direction=RIGHT") 1 1 1 1 "4744..5199") 
COMMENT        #19=(CFSignal (CObList) "L4440" 28 0 0 4227 4238 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4636..4647") 
COMMENT        #20=(CFSignal (CObList) "L4440" 28 0 1 4166 4177 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4575..4586)") 
COMMENT        #21=(CFSignal (CObList) "AAV2 ITR" 34 0 0 4120 4249 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4529..4658") 
COMMENT        #22=(CFSignal (CObList) "AAV2 ITR" 34 0 0 4120 4260 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4529..4669") 
COMMENT        #23=(CFSignal (CObList) "hGH-PA-R" 28 0 1 3803 3822 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4212..4231)") 
COMMENT        #24=(CFSignal (CObList) "hGH poly(A) signal" 25 0 0 3604 4080 1 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4013..4489") 
COMMENT        #25=(CFSignal (CObList) "Factor Xa site" 4 0 1 3455 3466 1 (CStringList) 
COMMENT             (CStringList "/codon_start=1" 
COMMENT              "/product=\"Factor Xa recognition and cleavage site\"") 1 1 1 1 
COMMENT             "complement(3864..3875)") 
COMMENT        #26=(CFSignal (CObList) "WPRE-R" 28 0 1 3037 3057 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3446..3466)") 
COMMENT        #27=(CFSignal (CObList) "WPRE" 21 0 0 2984 3572 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3393..3981") 
COMMENT        #28=(CFSignal (CObList) "FRT (minimal)" 31 0 1 2842 2875 1 (CStringList) 
COMMENT             (CStringList 
COMMENT              "/bound_moiety=\"FLP recombinase from the Saccharomyces" 
COMMENT              "cerevisiae 2u plasmid\"") 1 1 1 1 "complement(3251..3284)") 
COMMENT        #29=(CFSignal (CObList) "FRT (minimal)" 31 0 0 1450 1483 1 (CStringList) 
COMMENT             (CStringList 
COMMENT              "/bound_moiety=\"FLP recombinase from the Saccharomyces" 
COMMENT              "cerevisiae 2u plasmid\"") 1 1 1 1 "1450..1483") 
COMMENT        #30=(CFSignal (CObList) "EF1a-F" 28 0 0 1367 1387 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1367..1387") 
COMMENT        #31=(CFSignal (CObList) "EF-1-alpha intron A" 15 0 0 473 1410 1 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "473..1410") 
COMMENT        #32=(CFSignal (CObList) "EF-1-alpha promoter" 30 0 0 242 1419 1 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "242..1419") 
COMMENT        #33=(CFSignal (CObList) "L4440" 28 0 0 84 95 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "84..95") 
COMMENT        #34=(CFSignal (CObList) "L4440" 28 0 1 23 34 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(23..34)") 
COMMENT        #35=(CFSignal (CObList) "AAV2 ITR" 34 0 0 12 141 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "12..141") 
COMMENT        #36=(CFSignal (CObList) "AAV2 ITR" 34 0 0 1 141 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1..141") 
COMMENT        #37=(CFSignal (CObList) "Kozak" 21 0 1 2830 2835 1 (CStringList) 
COMMENT             (CStringList "/ApEinfo_fwdcolor=\"#d6b295\"" 
COMMENT              "/ApEinfo_revcolor=\"#d6b295\"" 
COMMENT              "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT              "width 5 offset 0\"") 1 1 1 1 "complement(1263..1268)") 
COMMENT        #38=(CFSignal (CObList) "2x stop" 21 0 1 1576 1581 1 (CStringList) 
COMMENT             (CStringList "/ApEinfo_fwdcolor=\"#ff8000\"" 
COMMENT              "/ApEinfo_revcolor=\"#ff8000\"" 
COMMENT              "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT              "width 5 offset 0\"") 1 1 1 1 "complement(9..14)") 
COMMENT        #39=(CFSignal (CObList) "GCaMP6s" 21 0 1 1582 2829 1 (CStringList) 
COMMENT             (CStringList "/ApEinfo_fwdcolor=\"cyan\"" 
COMMENT              "/ApEinfo_revcolor=\"green\"" 
COMMENT              "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT              "width 5 offset 0\"") 1 1 1 1 "complement(15..1262)")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #40=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #41=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) 
COMMENT                    "pAAV-Ef1a-fDIO-GCaMP6s" 2) 5 "" 0 4) 
COMMENT              #42=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #43=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA 'pAAV-Ef1a-fDIO-GCaMP6s'" 1) 
COMMENT                     #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #45=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 07/28/17 06:18PM" 1) 
COMMENT                     #46=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 07/28/17 06:18PM" 1) 
COMMENT                     #47=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6857 bp" 1) 
COMMENT                     #48=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Designed" 1) 
COMMENT                     #49=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #50=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) (CObjectList)) 
COMMENT                   "Standard Fields") 
COMMENT              #51=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #52=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) 
COMMENT                   "Annotations") 
COMMENT              #53=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 10 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #54=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 0 (5 #1# 0) 1 2 1 0 195) 
COMMENT                           (CObjectList 
COMMENT                            #55=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "parent position: from 3246 to 1569" 1) 
COMMENT                            #56=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "original length: 5590" 1) 
COMMENT                            #57=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "molecule position: from 2837 to 1569" 1) 
COMMENT                            #58=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) 
COMMENT                                  (CObjectList 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  NheI site #1" 1))) "Left Terminus") 
COMMENT                            #60=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) 
COMMENT                                  (CObjectList 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  AscI site #1" 1))) "Right Terminus"))) 
COMMENT                          "#1: FRAGMENT of <a href=\"vnti:dnarna/pAAV-Ef1a-fDIO%20hChR2%28H134R%29-EYFP?sel=3246-1569\">pAAV-Ef1a-fDIO hChR2(H134R)-EYFP</a> ") 
COMMENT                     #62=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 0 (5 #2# 0) 1 2 1 0 195) 
COMMENT                           (CObjectList 
COMMENT                            #63=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "parent position: from 3 to 1269" 1) 
COMMENT                            #64=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "original length: 1267" 1) 
COMMENT                            #65=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "molecule position: from 1570 to 2836" 1) 
COMMENT                            #66=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) 
COMMENT                                  (CObjectList 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  AscI site #1" 1))) "Left Terminus") 
COMMENT                            #68=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) 
COMMENT                                  (CObjectList 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  NheI site #1" 1))) "Right Terminus"))) 
COMMENT                          "#2: FRAGMENT of <a href=\"vnti:dnarna/GCaMP6s_RC_insert_forcloning?sel=3-1269\">GCaMP6s_RC_insert_forcloning</a> "))) 
COMMENT                   "Component Fragments") 
COMMENT              #70=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 11 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #71=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 0 (0 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #72=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Result Molecule: pAAV-Ef1a-fDIO-GCaMP6s" 1) 
COMMENT                            #73=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "" 1) 
COMMENT                            #74=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Recipient: pAAV-Ef1a-fDIO hChR2(H134R)-EYFP" 1) 
COMMENT                            #75=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "  Left Terminus: NheI #1 (total 1)" 1) 
COMMENT                            #76=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "  Right Terminus: AscI #1 (total 1)" 1) 
COMMENT                            #77=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Full digestion" 1) 
COMMENT                            #78=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "" 1) 
COMMENT                            #79=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Donor: GCaMP6s_RC_insert_forcloning " 1) 
COMMENT                            #80=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "  Left Terminus: AscI #1 (total 1)" 1) 
COMMENT                            #81=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "    Flank Region: 0" 1) 
COMMENT                            #82=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "  Right Terminus: NheI #1 (total 1)" 1) 
COMMENT                            #83=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "    Flank Region: 0" 1) 
COMMENT                            #84=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Full digestion" 1) 
COMMENT                            #85=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "" 1) 
COMMENT                            #86=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Ligation: cohesive termini at both junctions" 
COMMENT                                 1) 
COMMENT                            #87=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Required orientation achieved" 1) 
COMMENT                            #88=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "" 1) 
COMMENT                            #89=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Transformation system: Bacteria" 1) 
COMMENT                            #90=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Extra-chromosomal replication" 1) 
COMMENT                            #91=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "" 1) 
COMMENT                            #92=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Recommended method(s) for clone analysis:" 1) 
COMMENT                            #93=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "   Restriction Analysis" 1) 
COMMENT                            #94=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "" 1) 
COMMENT                            #95=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Restriction analysis:" 1) 
COMMENT                            #96=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "   recombinant has new restriction site BclI" 
COMMENT                                 1) 
COMMENT                            #97=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "" 1) 
COMMENT                            #98=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Recommended oligonucleotide for colony hybridization" 
COMMENT                                 1) 
COMMENT                            #99=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "of cloned fragment in resulting molecule:" 1) 
COMMENT                            #100=(COlyLinePar 
COMMENT                                  (CLinePar 
COMMENT                                   (CParagraph 0 
COMMENT                                    (17 
COMMENT                                     #101=(COligo "" "CGCGCCTTACTACTTCGCTG" "" 0 
COMMENT                                           (CStringList) 0) 0) 1 2 2 0 181) 
COMMENT                                   " CGCGCCTTACTACTTCGCTG" 1)) 
COMMENT                            #102=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "" 1) 
COMMENT                            #103=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                  "Important Restriction Sites:" 1) 
COMMENT                            #104=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                  "Cloned fragment may be cut by AscI and NheI" 
COMMENT                                  1) 
COMMENT                            #105=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                  "Recombinant lacks sites of:" 1) 
COMMENT                            #106=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                  " AarI AsiSI AvrII BaeI BsaBI BsiWI" 1) 
COMMENT                            #107=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                  " BsmI BstZ17I Bsu36I FspAI M.BaeI M.EcoAI" 1) 
COMMENT                            #108=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                  " M.KpnAI M.KpnBI M.NgoAV M.PshAI M.SnaBI M.Tth111I" 
COMMENT                                  1) 
COMMENT                            #109=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                  " M.XcmI NdeI NruI PacI PmeI PpuMI" 1) 
COMMENT                            #110=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                  " PshAI PsrI SanDI SexAI SfiI SgrAI" 1) 
COMMENT                            #111=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                  " SnaBI SpeI Sse232I Sse8647I SwaI Tth111I" 1) 
COMMENT                            #112=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                  " XcmI" 1) 
COMMENT                            #113=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                  "Unique sites inside cloned fragment:" 1) 
COMMENT                            #114=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                  " AscI BmgBI HpaI M.EcoBI M.HpaI NcoI SapI" 1) 
COMMENT                            #115=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                  "Unique sites outside cloned fragment:" 1) 
COMMENT                            #116=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                  " NheI AfeI AflII AgeI AlwNI ApaBI ApaI AseI" 
COMMENT                                  1) 
COMMENT                            #117=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                  " BamHI BfrBI BmtI BspEI BstAPI BstEII EcoRI EcoRV" 
COMMENT                                  1) 
COMMENT                            #118=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                  " FseI FspI M.ApaI M.BnaI M.BstI M.EcaI M.EcoKI M.EcoRI" 
COMMENT                                  1) 
COMMENT                            #119=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
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COMMENT                                            "Start: 1450  End: 1483" 1) 
COMMENT                                      #271=(CLinePar 
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COMMENT                                            "   1450..1483" 1) 
COMMENT                                      #274=(CLinePar 
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COMMENT                                            "   cerevisiae 2u plasmid\"" 1))) 
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COMMENT                                            "Start: 2842  End: 2875 (Complementary)" 
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COMMENT                                      #279=(CLinePar 
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COMMENT                                            "   complement(3251..3284)" 1) 
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COMMENT                                            "   cerevisiae 2u plasmid\"" 1))) 
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COMMENT                                            "Start: 4335  End: 4790" 1) 
COMMENT                                      #288=(CLinePar 
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COMMENT                                            "Start: 6208  End: 6796" 1) 
COMMENT                                      #295=(CLinePar 
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COMMENT                            "Repeat Region (4 total)"))) "Feature Map") 
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COMMENT          #334=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 
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FEATURES             Location/Qualifiers
     primer_bind     6697..6716
                     /vntifkey="28"
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      6208..6796
                     /direction=RIGHT
                     /vntifkey="33"
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     primer_bind     complement(5395..5414)
                     /vntifkey="28"
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             5177..6037
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /vntifkey="4"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        5072..5176
                     /gene="bla"
                     /vntifkey="30"
                     /label=AmpR\promoter
     primer_bind     complement(4986..5004)
                     /vntifkey="28"
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward primer"
     primer_bind     4926..4948
                     /vntifkey="28"
                     /label=pGEX\3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4807..4826)
                     /vntifkey="28"
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable marker"
     primer_bind     4632..4653
                     /vntifkey="28"
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4422..4441)
                     /vntifkey="28"
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      4335..4790
                     /direction=RIGHT
                     /vntifkey="33"
                     /label=f1\ori
                     /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
     primer_bind     4227..4238
                     /vntifkey="28"
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4166..4177)
                     /vntifkey="28"
                     /label=L4440
                     /note="L4440 vector, forward primer"
     repeat_region   4120..4249
                     /vntifkey="34"
                     /label=AAV2\ITR
     repeat_region   4120..4260
                     /vntifkey="34"
                     /label=AAV2\ITR
                     /note="inverted terminal repeat of adeno-associated virus serotype 2"
     primer_bind     complement(3803..3822)
                     /vntifkey="28"
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     polyA_signal    3604..4080
                     /vntifkey="25"
                     /label=hGH\poly(A)\signal
                     /note="human growth hormone polyadenylation signal"
     CDS             complement(3455..3466)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /vntifkey="4"
                     /label=Factor\Xa\site
     primer_bind     complement(3037..3057)
                     /vntifkey="28"
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     misc_feature    2984..3572
                     /vntifkey="21"
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional regulatory element"
     protein_bind    complement(2842..2875)
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /vntifkey="31"
                     /label=FRT\(minimal)
                     /note="supports FLP-mediated excision but not integration (Turan and Bode, 2011)"
     protein_bind    1450..1483
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /vntifkey="31"
                     /label=FRT\(minimal)
                     /note="supports FLP-mediated excision but not integration (Turan and Bode, 2011)"
     primer_bind     1367..1387
                     /vntifkey="28"
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     intron          473..1410
                     /vntifkey="15"
                     /label=EF-1-alpha\intron\A
                     /note="intron upstream of the start codon of human EF-1-alpha"
     promoter        242..1419
                     /vntifkey="30"
                     /label=EF-1-alpha\promoter
                     /note="strong constitutive promoter for human elongation factor EF-1-alpha"
     primer_bind     84..95
                     /vntifkey="28"
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(23..34)
                     /vntifkey="28"
                     /label=L4440
                     /note="L4440 vector, forward primer"
     repeat_region   12..141
                     /vntifkey="34"
                     /label=AAV2\ITR
     repeat_region   1..141
                     /vntifkey="34"
                     /label=AAV2\ITR
                     /note="inverted terminal repeat of adeno-associated virus serotype 2"
     misc_feature    complement(2830..2835)
                     /ApEinfo_fwdcolor="#d6b295"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=Kozak
     misc_feature    complement(1576..1581)
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=2x\stop
     misc_feature    complement(1582..2829)
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=GCaMP6s
BASE COUNT     1371 a      1837 c      1853 g      1796 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt 
      181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat 
      241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg 
      301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag 
      361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc 
      421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc 
      481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat 
      541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 
      601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 
      661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 
      721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 
      781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 
      841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 
      901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 
      961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 
     1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 
     1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 
     1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 
     1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 
     1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 
     1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 
     1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gtaccggatc ctctagagtc 
     1441 gactccggag aagttcctat tctctagaaa gtataggaac ttcgcagaat ggtagctgga 
     1501 ttgtagctgc tattagcaat atgaaacctc ttagaagttc ctattcttca aaaggtatag 
     1561 gaacttcggc gcgccttact acttcgctgt catcatttgt acaaactctt cgtagtttac 
     1621 ctgaccatcc ccatcgatgt ctgcttccct gatcatttca tcaacctctt catctgttaa 
     1681 cttctctcca aggtttgtca tcacgtggcg aagctctgct gcactgatgt agccattgcc 
     1741 atccttatca aacacaccga acgcttctct aatttcttct tccgtgtccc tgtatttcat 
     1801 ttttcttgcc atcattgtca ggaactcagg gaagtcgatt gtgccgtcac cgtcggcatc 
     1861 tacttcattg atcatgtcct gcagctctgc ttctgtgggg ttctgcccca gagaccgcat 
     1921 caccgtcccc agctccttgg ttgttattgt cccatccccg tccttgtcaa atagggagaa 
     1981 agcctcttta aattctgcga tctgctcttc agtcagttgg tccggcaggt tgtactccag 
     2041 cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt 
     2101 caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt 
     2161 gaagaagatg gtgcgctcct ggatgtagcc ttcgggcatg gcggacttga agaagtcgtg 
     2221 ctgcttcatg tggtcggggt agcggctgaa gcactgcacg ccgtaggtca gggtggtcac 
     2281 gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt 
     2341 gccgtaggtg gcatcgccct caccctcgcc ggacacgctg aacttgtggc cgtttacgtc 
     2401 gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac 
     2461 catgctccct ccggtaccgc ccttgtacag ctcgtccatg ccgagagtga tcccggcggc 
     2521 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tcgaaagttt 
     2581 ggactgcacg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt 
     2641 gttctgctgg tagtggtagg cgagctgcac gccgccgtcc tcgatgttgt ggcggatgtg 
     2701 gaagttcgcc ttgatgccgt tcttctgctt gtcggccttg atatagacgt tctcgagtga 
     2761 gctcagccga cctatagctc tgactgcgtg acctgtctta ttccacttac gacgtgatga 
     2821 gtcgaccatg gtggcgctag cgaagttcct atactttcta gagaatagga acttcttgcc 
     2881 ttaacccaga aattatcact gttattcttt agaatggtgc aaagagaagt tcctatacct 
     2941 tttgaagaat aggaacttcg aattcgatat caagcttatc gataatcaac ctctggatta 
     3001 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 
     3061 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc 
     3121 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca 
     3181 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac 
     3241 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact 
     3301 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc 
     3361 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctatg ttgccacctg 
     3421 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc 
     3481 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac 
     3541 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc 
     3601 tacgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact 
     3661 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg 
     3721 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag 
     3781 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct 
     3841 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag 
     3901 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga 
     3961 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca 
     4021 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt 
     4081 ctgattttgt aggtaaccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt 
     4141 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc 
     4201 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg 
     4261 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc 
     4321 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 
     4381 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 
     4441 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 
     4501 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg 
     4561 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 
     4621 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta 
     4681 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 
     4741 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac 
     4801 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc 
     4861 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac 
     4921 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg 
     4981 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta 
     5041 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 
     5101 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 
     5161 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 
     5221 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 
     5281 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 
     5341 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 
     5401 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 
     5461 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 
     5521 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 
     5581 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 
     5641 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 
     5701 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 
     5761 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 
     5821 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 
     5881 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 
     5941 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 
     6001 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 
     6061 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 
     6121 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 
     6181 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 
     6241 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 
     6301 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 
     6361 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 
     6421 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 
     6481 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 
     6541 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 
     6601 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 
     6661 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 
     6721 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 
     6781 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 
     6841 gccttttgct cacatgt 
//
