LOCUS Exported 5202 bp ds-DNA circular SYN 14-PAŹ-2016 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS pKK-TEV-FLAG SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5202) AUTHORS . TITLE Direct Submission JOURNAL Exported 14 paza 2016 from SnapGene 3.0.3 http://www.snapgene.com COMMENT LSOWNER| FEATURES Location/Qualifiers source 1..5202 /organism="synthetic DNA construct" /mol_type="other DNA" source 1090..1116 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 232..958 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" protein_bind 820..838 /bound_moiety="repressor" /note="tet operator" protein_bind 841..859 /bound_moiety="repressor" /note="tet operator" misc_feature 1002..1022 /note="TEV-L" /note="enocdes peptide cleaved by TEV protease" misc_feature 1063..1083 /note="TEV-R" /note="enocdes peptide cleaved by TEV protease" CDS 1090..1116 /codon_start=1 /note="FLAG" /translation="DYKDDDDK" polyA_signal 1160..1384 /note="BGH poly(A)signal" /note="bovine growth hormone polyadenylation signal" protein_bind 1668..1715 /bound_moiety="FLP recombinase" /note="FRT" CDS 1723..2745 /codon_start=1 /product="aminoglycoside phosphotransferase from E. coli" /note="Hygromycin B" /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE " polyA_signal 2875..3005 /note="SV40 poly(A)signal" /note="SV40 polyadenylation signal" rep_origin complement(3388..4061) /direction=LEFT /note="pUC ori" CDS complement(4206..5066) /codon_start=1 /product="beta-lactamase" /note="AmpR" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" misc_feature complement(5067..5165) /note="Amp promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc 841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga 901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga 961 ctctggcgtt taaacttaag cttggtaccg agctcggatc cgaaaacctg tacttccaag 1021 gaaccggtgg tcacctgtac aatcgatcct gcaggagcta gcgagaattt gtattttcag 1081 ggtgatatcg attacaagga tgacgacgat aagtagctcg agtctagagg gcccgtttaa 1141 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc 1201 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag 1261 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag 1321 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct 1381 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt 1441 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 1501 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 1561 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 1621 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtacctagaa gttcctattc 1681 cgaagttcct attctctaga aagtatagga acttccttgg ccaaaaagcc tgaactcacc 1741 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag 1801 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc 1861 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt 1921 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg 1981 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa 2041 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt 2101 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg 2161 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac 2221 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac 2281 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac 2341 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac 2401 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc 2461 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc 2521 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct 2581 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca 2641 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat 2701 agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagcacgt actacgagat 2761 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc 2821 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg 2881 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 2941 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 3001 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct 3061 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 3121 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 3181 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 3241 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 3301 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 3361 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 3421 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 3481 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 3541 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 3601 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 3661 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 3721 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 3781 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 3841 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 3901 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 3961 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 4021 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 4081 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 4141 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 4201 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 4261 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 4321 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 4381 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 4441 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 4501 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 4561 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 4621 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 4681 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 4741 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 4801 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 4861 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 4921 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 4981 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 5041 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 5101 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 5161 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc //